SEED LONGEVITY AND CRYOBIOTECHNOLOGY IN THE ORCHID GENUS Cattleya
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/218742 |
Resumo: | BACKGROUND: Orchid seeds are reputed to be relatively short-lived, although comparative studies across a wide range of storage temperatures and moisture contents are few. OBJECTIVE: To explore how a wide range of temperature and moisture contents affects seed longevity in the orchid genus Cattleya. MATERIALS AND METHODS: Seeds of three Brazilian orchid species in the genus Cattleya (C. amethystoglossa, C. kautskyana and C. tigrina) were subjected to controlled deterioration (CD) and seeds at three moisture levels were stored for up to 270 days at temperatures as low as -196 degrees C. Seed ageing curves were constructed and seed quality assessed as germination level and speed index in vitro, and by tetrazolium staining. Seed morphometry, lipid content and composition were also determined. RESULTS: Seeds were found to be lipid rich (54-70% DW) and short-lived under CD, with P(50)s varying less than two-fold (42 to 69 h) at 41 degrees C. At cold (5 degrees C) to ultracold (-196 degrees C) temperatures longevity was greatest after pre-drying seeds to 15% RH, with germination varying by c. 6% after 270 days storage at these temperature limits. However, storage at -20 degrees C resulted in a 5-46% reduction in germination, indicating a dry-seed sensitivity to conventional seed bank temperature. Ultra-dry (silica gel) and high humidity (82% RH) storage, in combination with various temperatures, compromised viability in one or more species. Overall, seed longevity in C. kautskyana > C. tigrina > C. amethystoglossa, which also reflected the pattern of embryo volume. The prospect that the generally shorter lifespans in orchid seeds may relate to stochastic processes the negative impact of which reach criticality sooner in smaller embryos requires further testing. CONCLUSION: Further evidence is provided that cryobiotechnology provides a biobanking option for orchid seeds with relatively short lifespans. |
id |
UNSP_996ff7fcdcf3cd70ada2cc4516b5c92b |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/218742 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
SEED LONGEVITY AND CRYOBIOTECHNOLOGY IN THE ORCHID GENUS CattleyaconservationcryopreservationlongevityOrchidaceaeseed bankingseed lipid contentstorageBACKGROUND: Orchid seeds are reputed to be relatively short-lived, although comparative studies across a wide range of storage temperatures and moisture contents are few. OBJECTIVE: To explore how a wide range of temperature and moisture contents affects seed longevity in the orchid genus Cattleya. MATERIALS AND METHODS: Seeds of three Brazilian orchid species in the genus Cattleya (C. amethystoglossa, C. kautskyana and C. tigrina) were subjected to controlled deterioration (CD) and seeds at three moisture levels were stored for up to 270 days at temperatures as low as -196 degrees C. Seed ageing curves were constructed and seed quality assessed as germination level and speed index in vitro, and by tetrazolium staining. Seed morphometry, lipid content and composition were also determined. RESULTS: Seeds were found to be lipid rich (54-70% DW) and short-lived under CD, with P(50)s varying less than two-fold (42 to 69 h) at 41 degrees C. At cold (5 degrees C) to ultracold (-196 degrees C) temperatures longevity was greatest after pre-drying seeds to 15% RH, with germination varying by c. 6% after 270 days storage at these temperature limits. However, storage at -20 degrees C resulted in a 5-46% reduction in germination, indicating a dry-seed sensitivity to conventional seed bank temperature. Ultra-dry (silica gel) and high humidity (82% RH) storage, in combination with various temperatures, compromised viability in one or more species. Overall, seed longevity in C. kautskyana > C. tigrina > C. amethystoglossa, which also reflected the pattern of embryo volume. The prospect that the generally shorter lifespans in orchid seeds may relate to stochastic processes the negative impact of which reach criticality sooner in smaller embryos requires further testing. CONCLUSION: Further evidence is provided that cryobiotechnology provides a biobanking option for orchid seeds with relatively short lifespans.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Biol Coll, Unoeste Campus 2,Rod Raposo Tavares Km 572, BR-19067175 Presidente Prudente, SP, BrazilUNESP Inst Quim Araraquara IQAr, Dept Quim Analit, Rodovia Araraquara Jau,Km 1, BR-14800901 Araraquara, SP, BrazilRoyal Bot Gardens, Ardingly RH17 6TN, W Sussex, EnglandChinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R ChinaAgron Coll, Unoeste Campus 2,Rodovia Raposo Tavares,Km 572, BR-19067175 Presidente Prudente, SP, BrazilUNESP Inst Quim Araraquara IQAr, Dept Quim Analit, Rodovia Araraquara Jau,Km 1, BR-14800901 Araraquara, SP, BrazilCryo LettersBiol CollUniversidade Estadual Paulista (UNESP)Royal Bot GardensChinese Acad SciAgron CollFileti, Jessica FontesHengling, Mariana M.Gianeti, Thiago M. R. [UNESP]Pritchard, Hugh W.Hosomi, Silverio T.Machado-Neto, Nelson B.Custodio, Ceci C.2022-04-28T17:22:44Z2022-04-28T17:22:44Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article353-365Cryoletters. London: Cryo Letters, v. 42, n. 6, p. 353-365, 2021.0143-2044http://hdl.handle.net/11449/218742WOS:000732574400006Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCryolettersinfo:eu-repo/semantics/openAccess2022-04-28T17:22:44Zoai:repositorio.unesp.br:11449/218742Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:00:48.307239Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
SEED LONGEVITY AND CRYOBIOTECHNOLOGY IN THE ORCHID GENUS Cattleya |
title |
SEED LONGEVITY AND CRYOBIOTECHNOLOGY IN THE ORCHID GENUS Cattleya |
spellingShingle |
SEED LONGEVITY AND CRYOBIOTECHNOLOGY IN THE ORCHID GENUS Cattleya Fileti, Jessica Fontes conservation cryopreservation longevity Orchidaceae seed banking seed lipid content storage |
title_short |
SEED LONGEVITY AND CRYOBIOTECHNOLOGY IN THE ORCHID GENUS Cattleya |
title_full |
SEED LONGEVITY AND CRYOBIOTECHNOLOGY IN THE ORCHID GENUS Cattleya |
title_fullStr |
SEED LONGEVITY AND CRYOBIOTECHNOLOGY IN THE ORCHID GENUS Cattleya |
title_full_unstemmed |
SEED LONGEVITY AND CRYOBIOTECHNOLOGY IN THE ORCHID GENUS Cattleya |
title_sort |
SEED LONGEVITY AND CRYOBIOTECHNOLOGY IN THE ORCHID GENUS Cattleya |
author |
Fileti, Jessica Fontes |
author_facet |
Fileti, Jessica Fontes Hengling, Mariana M. Gianeti, Thiago M. R. [UNESP] Pritchard, Hugh W. Hosomi, Silverio T. Machado-Neto, Nelson B. Custodio, Ceci C. |
author_role |
author |
author2 |
Hengling, Mariana M. Gianeti, Thiago M. R. [UNESP] Pritchard, Hugh W. Hosomi, Silverio T. Machado-Neto, Nelson B. Custodio, Ceci C. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Biol Coll Universidade Estadual Paulista (UNESP) Royal Bot Gardens Chinese Acad Sci Agron Coll |
dc.contributor.author.fl_str_mv |
Fileti, Jessica Fontes Hengling, Mariana M. Gianeti, Thiago M. R. [UNESP] Pritchard, Hugh W. Hosomi, Silverio T. Machado-Neto, Nelson B. Custodio, Ceci C. |
dc.subject.por.fl_str_mv |
conservation cryopreservation longevity Orchidaceae seed banking seed lipid content storage |
topic |
conservation cryopreservation longevity Orchidaceae seed banking seed lipid content storage |
description |
BACKGROUND: Orchid seeds are reputed to be relatively short-lived, although comparative studies across a wide range of storage temperatures and moisture contents are few. OBJECTIVE: To explore how a wide range of temperature and moisture contents affects seed longevity in the orchid genus Cattleya. MATERIALS AND METHODS: Seeds of three Brazilian orchid species in the genus Cattleya (C. amethystoglossa, C. kautskyana and C. tigrina) were subjected to controlled deterioration (CD) and seeds at three moisture levels were stored for up to 270 days at temperatures as low as -196 degrees C. Seed ageing curves were constructed and seed quality assessed as germination level and speed index in vitro, and by tetrazolium staining. Seed morphometry, lipid content and composition were also determined. RESULTS: Seeds were found to be lipid rich (54-70% DW) and short-lived under CD, with P(50)s varying less than two-fold (42 to 69 h) at 41 degrees C. At cold (5 degrees C) to ultracold (-196 degrees C) temperatures longevity was greatest after pre-drying seeds to 15% RH, with germination varying by c. 6% after 270 days storage at these temperature limits. However, storage at -20 degrees C resulted in a 5-46% reduction in germination, indicating a dry-seed sensitivity to conventional seed bank temperature. Ultra-dry (silica gel) and high humidity (82% RH) storage, in combination with various temperatures, compromised viability in one or more species. Overall, seed longevity in C. kautskyana > C. tigrina > C. amethystoglossa, which also reflected the pattern of embryo volume. The prospect that the generally shorter lifespans in orchid seeds may relate to stochastic processes the negative impact of which reach criticality sooner in smaller embryos requires further testing. CONCLUSION: Further evidence is provided that cryobiotechnology provides a biobanking option for orchid seeds with relatively short lifespans. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-01 2022-04-28T17:22:44Z 2022-04-28T17:22:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Cryoletters. London: Cryo Letters, v. 42, n. 6, p. 353-365, 2021. 0143-2044 http://hdl.handle.net/11449/218742 WOS:000732574400006 |
identifier_str_mv |
Cryoletters. London: Cryo Letters, v. 42, n. 6, p. 353-365, 2021. 0143-2044 WOS:000732574400006 |
url |
http://hdl.handle.net/11449/218742 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cryoletters |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
353-365 |
dc.publisher.none.fl_str_mv |
Cryo Letters |
publisher.none.fl_str_mv |
Cryo Letters |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129572721393664 |