Different doses of exogenous surfactant for treatment of meconium aspiration syndrome in newborn rabbits.
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0041-87812004000300003 http://hdl.handle.net/11449/67749 |
Resumo: | OBJECTIVE: To evaluate the effects of 2 different doses of exogenous surfactant on pulmonary mechanics and on the regularity of pulmonary parenchyma inflation in newborn rabbits. METHOD: Newborn rabbits were submitted to tracheostomy and randomized into 4 study groups: the Control group did not receive any material inside the trachea; the MEC group was instilled with meconium, without surfactant treatment; the S100 and S200 groups were instilled with meconium and were treated with 100 and 200 mg/kg of exogenous surfactant (produced by Instituto Butantan) respectively. Animals from the 4 groups were mechanically ventilated during a 25-minute period. Dynamic compliance, ventilatory pressure, tidal volume, and maximum lung volume (P-V curve) were evaluated. Histological analysis was conducted using the mean linear intercept (Lm), and the lung tissue distortion index (SDI) was derived from the standard deviation of the means of the Lm. One-way analysis of variance was used with a = 0.05. RESULTS: After 25 minutes of ventilation, dynamic compliance (mL/cm H2O.kg) was 0.87 +/- 0.07 (Control); 0.49 +/- 0.04 (MEC*); 0.67 +/- 0.06 (S100); and 0.67 +/- 0.08 (S200), and ventilatory pressure (cm H2O) was 9.0 +/- 0.9 (Control); 16.5 +/- 1.7 (MEC*); 12.4 +/- 1.1 (S100); and 12.1 +/- 1.5 (S200). Both treated groups had lower Lm values and more homogeneity in the lung parenchyma compared to the MEC group: SDI = 7.5 +/- 1.9 (Control); 11.3 +/- 2.5 (MEC*), 5.8 +/- 1.9 (S100); and 6.7 +/- 1.7 (S200) (*P < 0.05 versus all the other groups). CONCLUSIONS: Animals treated with surfactant showed significant improvement in pulmonary mechanics and more regularity of the lung parenchyma in comparison to untreated animals. There was no difference in results after treatment with either of the doses used. |
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Different doses of exogenous surfactant for treatment of meconium aspiration syndrome in newborn rabbits.lung surfactantanimalartificial ventilationbreathing mechanicscomparative studydisease modeldrug effectfemalehumanlung compliancelung gas exchangemalemeconium aspirationnewbornrabbittimeAnimalsAnimals, NewbornDisease Models, AnimalFemaleHumansInfant, NewbornLung ComplianceMaleMeconium Aspiration SyndromePulmonary Gas ExchangePulmonary SurfactantsRabbitsRespiration, ArtificialRespiratory MechanicsTime FactorsOBJECTIVE: To evaluate the effects of 2 different doses of exogenous surfactant on pulmonary mechanics and on the regularity of pulmonary parenchyma inflation in newborn rabbits. METHOD: Newborn rabbits were submitted to tracheostomy and randomized into 4 study groups: the Control group did not receive any material inside the trachea; the MEC group was instilled with meconium, without surfactant treatment; the S100 and S200 groups were instilled with meconium and were treated with 100 and 200 mg/kg of exogenous surfactant (produced by Instituto Butantan) respectively. Animals from the 4 groups were mechanically ventilated during a 25-minute period. Dynamic compliance, ventilatory pressure, tidal volume, and maximum lung volume (P-V curve) were evaluated. Histological analysis was conducted using the mean linear intercept (Lm), and the lung tissue distortion index (SDI) was derived from the standard deviation of the means of the Lm. One-way analysis of variance was used with a = 0.05. RESULTS: After 25 minutes of ventilation, dynamic compliance (mL/cm H2O.kg) was 0.87 +/- 0.07 (Control); 0.49 +/- 0.04 (MEC*); 0.67 +/- 0.06 (S100); and 0.67 +/- 0.08 (S200), and ventilatory pressure (cm H2O) was 9.0 +/- 0.9 (Control); 16.5 +/- 1.7 (MEC*); 12.4 +/- 1.1 (S100); and 12.1 +/- 1.5 (S200). Both treated groups had lower Lm values and more homogeneity in the lung parenchyma compared to the MEC group: SDI = 7.5 +/- 1.9 (Control); 11.3 +/- 2.5 (MEC*), 5.8 +/- 1.9 (S100); and 6.7 +/- 1.7 (S200) (*P < 0.05 versus all the other groups). CONCLUSIONS: Animals treated with surfactant showed significant improvement in pulmonary mechanics and more regularity of the lung parenchyma in comparison to untreated animals. There was no difference in results after treatment with either of the doses used.Universidade Estadual Paulista (Unesp)Lyra, João CesarMascaretti, Renata SPrecioso, Alexander RobertoChang, Yin ChiaCosta, Maria Tereza Zulini daVaz, Flávio Adolfo CostaOkay, YassuhikoRebello, Celso Moura2014-05-27T11:21:05Z2014-05-27T11:21:05Z2004-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article104-112application/pdfhttp://dx.doi.org/10.1590/S0041-87812004000300003Revista do Hospital das Clinicas, v. 59, n. 3, p. 104-112, 2004.0041-8781http://hdl.handle.net/11449/6774910.1590/S0041-87812004000300003S0041-878120040003000032-s2.0-287444560672-s2.0-28744456067.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista do Hospital das Clínicasinfo:eu-repo/semantics/openAccess2023-11-04T06:07:50Zoai:repositorio.unesp.br:11449/67749Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-04T06:07:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Different doses of exogenous surfactant for treatment of meconium aspiration syndrome in newborn rabbits. |
title |
Different doses of exogenous surfactant for treatment of meconium aspiration syndrome in newborn rabbits. |
spellingShingle |
Different doses of exogenous surfactant for treatment of meconium aspiration syndrome in newborn rabbits. Lyra, João Cesar lung surfactant animal artificial ventilation breathing mechanics comparative study disease model drug effect female human lung compliance lung gas exchange male meconium aspiration newborn rabbit time Animals Animals, Newborn Disease Models, Animal Female Humans Infant, Newborn Lung Compliance Male Meconium Aspiration Syndrome Pulmonary Gas Exchange Pulmonary Surfactants Rabbits Respiration, Artificial Respiratory Mechanics Time Factors |
title_short |
Different doses of exogenous surfactant for treatment of meconium aspiration syndrome in newborn rabbits. |
title_full |
Different doses of exogenous surfactant for treatment of meconium aspiration syndrome in newborn rabbits. |
title_fullStr |
Different doses of exogenous surfactant for treatment of meconium aspiration syndrome in newborn rabbits. |
title_full_unstemmed |
Different doses of exogenous surfactant for treatment of meconium aspiration syndrome in newborn rabbits. |
title_sort |
Different doses of exogenous surfactant for treatment of meconium aspiration syndrome in newborn rabbits. |
author |
Lyra, João Cesar |
author_facet |
Lyra, João Cesar Mascaretti, Renata S Precioso, Alexander Roberto Chang, Yin Chia Costa, Maria Tereza Zulini da Vaz, Flávio Adolfo Costa Okay, Yassuhiko Rebello, Celso Moura |
author_role |
author |
author2 |
Mascaretti, Renata S Precioso, Alexander Roberto Chang, Yin Chia Costa, Maria Tereza Zulini da Vaz, Flávio Adolfo Costa Okay, Yassuhiko Rebello, Celso Moura |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Lyra, João Cesar Mascaretti, Renata S Precioso, Alexander Roberto Chang, Yin Chia Costa, Maria Tereza Zulini da Vaz, Flávio Adolfo Costa Okay, Yassuhiko Rebello, Celso Moura |
dc.subject.por.fl_str_mv |
lung surfactant animal artificial ventilation breathing mechanics comparative study disease model drug effect female human lung compliance lung gas exchange male meconium aspiration newborn rabbit time Animals Animals, Newborn Disease Models, Animal Female Humans Infant, Newborn Lung Compliance Male Meconium Aspiration Syndrome Pulmonary Gas Exchange Pulmonary Surfactants Rabbits Respiration, Artificial Respiratory Mechanics Time Factors |
topic |
lung surfactant animal artificial ventilation breathing mechanics comparative study disease model drug effect female human lung compliance lung gas exchange male meconium aspiration newborn rabbit time Animals Animals, Newborn Disease Models, Animal Female Humans Infant, Newborn Lung Compliance Male Meconium Aspiration Syndrome Pulmonary Gas Exchange Pulmonary Surfactants Rabbits Respiration, Artificial Respiratory Mechanics Time Factors |
description |
OBJECTIVE: To evaluate the effects of 2 different doses of exogenous surfactant on pulmonary mechanics and on the regularity of pulmonary parenchyma inflation in newborn rabbits. METHOD: Newborn rabbits were submitted to tracheostomy and randomized into 4 study groups: the Control group did not receive any material inside the trachea; the MEC group was instilled with meconium, without surfactant treatment; the S100 and S200 groups were instilled with meconium and were treated with 100 and 200 mg/kg of exogenous surfactant (produced by Instituto Butantan) respectively. Animals from the 4 groups were mechanically ventilated during a 25-minute period. Dynamic compliance, ventilatory pressure, tidal volume, and maximum lung volume (P-V curve) were evaluated. Histological analysis was conducted using the mean linear intercept (Lm), and the lung tissue distortion index (SDI) was derived from the standard deviation of the means of the Lm. One-way analysis of variance was used with a = 0.05. RESULTS: After 25 minutes of ventilation, dynamic compliance (mL/cm H2O.kg) was 0.87 +/- 0.07 (Control); 0.49 +/- 0.04 (MEC*); 0.67 +/- 0.06 (S100); and 0.67 +/- 0.08 (S200), and ventilatory pressure (cm H2O) was 9.0 +/- 0.9 (Control); 16.5 +/- 1.7 (MEC*); 12.4 +/- 1.1 (S100); and 12.1 +/- 1.5 (S200). Both treated groups had lower Lm values and more homogeneity in the lung parenchyma compared to the MEC group: SDI = 7.5 +/- 1.9 (Control); 11.3 +/- 2.5 (MEC*), 5.8 +/- 1.9 (S100); and 6.7 +/- 1.7 (S200) (*P < 0.05 versus all the other groups). CONCLUSIONS: Animals treated with surfactant showed significant improvement in pulmonary mechanics and more regularity of the lung parenchyma in comparison to untreated animals. There was no difference in results after treatment with either of the doses used. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-06-01 2014-05-27T11:21:05Z 2014-05-27T11:21:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0041-87812004000300003 Revista do Hospital das Clinicas, v. 59, n. 3, p. 104-112, 2004. 0041-8781 http://hdl.handle.net/11449/67749 10.1590/S0041-87812004000300003 S0041-87812004000300003 2-s2.0-28744456067 2-s2.0-28744456067.pdf |
url |
http://dx.doi.org/10.1590/S0041-87812004000300003 http://hdl.handle.net/11449/67749 |
identifier_str_mv |
Revista do Hospital das Clinicas, v. 59, n. 3, p. 104-112, 2004. 0041-8781 10.1590/S0041-87812004000300003 S0041-87812004000300003 2-s2.0-28744456067 2-s2.0-28744456067.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista do Hospital das Clínicas |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
104-112 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964812745113600 |