Leptospira spp. of the Urinary Tract of Female Carrier Goats in Semi-Arid Conditions
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.22456/1679-9216.124079 http://hdl.handle.net/11449/237573 |
Resumo: | Background: Leptospirosis is an important infectious disease in goat farming, with a worldwide distribution. It is usually transmitted by rodents and the genital route, may cause reproductive losses, negatively impacting goat fanning. The diagnosis lies on serological, molecular and isolation techniques. Considering the importance of this disease for small ruminants, this work aimed to evaluate the serological, molecular findings and isolation of pathogenic leptospires in the urinary tract (kidney and bladder tissues) of goats. Materials, Methods & Results: Thirty-four adult goats were used for slaughter. Renal samples (n = 34), bladder (n = 34), were collected for isolation of the agent and molecular detection of Leptospira sp. and blood samples (n = 34) for serological testing. The polymerase chain reaction (PCR) was used as a molecular test and the microscopic serum agglutination test (MAT) was used as a serological test. Samples with DNA amplification were subjected to genetic sequencing. The presence of Leptospira DNA was found in the tissues of 8 (23.4%) goats, and of these, only 2 were positive in PCR and MAL There was a slight agreement between the PCR and MAT techniques (k = 0.150; P = 0.436). In 6 (17.6%) samples of renal tissue and 2 (5.8%) bladder samples, Leptospira DNA was detected. The genes in a kidney tissue sample were sequenced and demonstrated 99% similarity to Leptospira interrogans. Anti-Leptospira sp. were detected in 6 (17.6%) of the animals tested. Discussion: Serology identified 3 predominant serogroups: Icterohaemorrhagiae, Tarassovi and Autumnalis, serogroups that are related to the presence of rodents that coexist in rural environments. Autumnalis has been reported in small ruminants, raising the hypothesis that goats are adapted, becoming chronic carriers and possible maintenance hosts. The frequency obtained (17.6%) may be the result of the mixed breed pattern and rustic characteristics inherent to the goat species. Given the characteristics of the semi-arid region, such as low rainfall and high solar incidence, it is essential to use an adapted methodology, with a lower cut-off point (1:50), as the serological titer is an established relationship between the animal species, the level of exposure throughout its evolution and the region studied. Molecular findings and bacterial isolation reveal the agent's ability to colonize the urinary tract of goats. These data show the importance that urine has in the epidemiological chain, being able to transmit the agent through direct contact with this product or through contamination of soil and water. There was no statistical agreement between the diagnostic techniques used in this study, in this case, an association between PCR and MAT is recommended to obtain data with high sensitivity and specificity. A bladder sample was sequenced and showed 99% similarity to Leptospira interrogans. In the semiarid region, the most common form of leptospirosis spread is through the sale of animals in business fairs for breeding, rearing or slaughter, as well as sharing the same property with several breeders. The introduction of chronic and asymptomatic carriers on the properties represents a serious risk for the spread of the disease. The results show the presence of Leptospira spp. in semi-arid goat herds, having as risk factors the presence of rodents and intercropping. The association of MAT and PCR is necessary for a better diagnosis of the disease. |
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Leptospira spp. of the Urinary Tract of Female Carrier Goats in Semi-Arid ConditionsLeptospirosisLeptospiresZoonosisSerologyMolecular detectionEpidemiologysemiaridBackground: Leptospirosis is an important infectious disease in goat farming, with a worldwide distribution. It is usually transmitted by rodents and the genital route, may cause reproductive losses, negatively impacting goat fanning. The diagnosis lies on serological, molecular and isolation techniques. Considering the importance of this disease for small ruminants, this work aimed to evaluate the serological, molecular findings and isolation of pathogenic leptospires in the urinary tract (kidney and bladder tissues) of goats. Materials, Methods & Results: Thirty-four adult goats were used for slaughter. Renal samples (n = 34), bladder (n = 34), were collected for isolation of the agent and molecular detection of Leptospira sp. and blood samples (n = 34) for serological testing. The polymerase chain reaction (PCR) was used as a molecular test and the microscopic serum agglutination test (MAT) was used as a serological test. Samples with DNA amplification were subjected to genetic sequencing. The presence of Leptospira DNA was found in the tissues of 8 (23.4%) goats, and of these, only 2 were positive in PCR and MAL There was a slight agreement between the PCR and MAT techniques (k = 0.150; P = 0.436). In 6 (17.6%) samples of renal tissue and 2 (5.8%) bladder samples, Leptospira DNA was detected. The genes in a kidney tissue sample were sequenced and demonstrated 99% similarity to Leptospira interrogans. Anti-Leptospira sp. were detected in 6 (17.6%) of the animals tested. Discussion: Serology identified 3 predominant serogroups: Icterohaemorrhagiae, Tarassovi and Autumnalis, serogroups that are related to the presence of rodents that coexist in rural environments. Autumnalis has been reported in small ruminants, raising the hypothesis that goats are adapted, becoming chronic carriers and possible maintenance hosts. The frequency obtained (17.6%) may be the result of the mixed breed pattern and rustic characteristics inherent to the goat species. Given the characteristics of the semi-arid region, such as low rainfall and high solar incidence, it is essential to use an adapted methodology, with a lower cut-off point (1:50), as the serological titer is an established relationship between the animal species, the level of exposure throughout its evolution and the region studied. Molecular findings and bacterial isolation reveal the agent's ability to colonize the urinary tract of goats. These data show the importance that urine has in the epidemiological chain, being able to transmit the agent through direct contact with this product or through contamination of soil and water. There was no statistical agreement between the diagnostic techniques used in this study, in this case, an association between PCR and MAT is recommended to obtain data with high sensitivity and specificity. A bladder sample was sequenced and showed 99% similarity to Leptospira interrogans. In the semiarid region, the most common form of leptospirosis spread is through the sale of animals in business fairs for breeding, rearing or slaughter, as well as sharing the same property with several breeders. The introduction of chronic and asymptomatic carriers on the properties represents a serious risk for the spread of the disease. The results show the presence of Leptospira spp. in semi-arid goat herds, having as risk factors the presence of rodents and intercropping. The association of MAT and PCR is necessary for a better diagnosis of the disease.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fed Univ Campina Grande UFCG, Palos, PB, BrazilUniv Estadual Paulista Unesp, Botucatu, SP, BrazilUniv Estadual Paulista Unesp, Botucatu, SP, BrazilCNPq: N 301029/2018-0Univ Fed Rio Grande Do SulFed Univ Campina Grande UFCGUniversidade Estadual Paulista (UNESP)Rocha, Laysa Mayara Soares BritoFaria, Pedro Jorge Alvares deSoares, Rafael RodriguesAraujo Junior, Joao Pessoa [UNESP]Malossi, Camila Dantas [UNESP]Ullmann, Leila Sabrina [UNESP]Silva, Maria Luana Cristiny RodriguesHigino, Severino Silvano dos SantosAzevedo, Sergio Santos deAlves, Clebert Jose2022-11-30T13:38:57Z2022-11-30T13:38:57Z2022-06-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8http://dx.doi.org/10.22456/1679-9216.124079Acta Scientiae Veterinariae. Porto Alegre Rs: Univ Fed Rio Grande Do Sul, v. 50, 8 p., 2022.1678-0345http://hdl.handle.net/11449/23757310.22456/1679-9216.124079WOS:000804934900001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Scientiae Veterinariaeinfo:eu-repo/semantics/openAccess2022-11-30T13:38:57Zoai:repositorio.unesp.br:11449/237573Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-05-23T11:30:10.252200Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Leptospira spp. of the Urinary Tract of Female Carrier Goats in Semi-Arid Conditions |
title |
Leptospira spp. of the Urinary Tract of Female Carrier Goats in Semi-Arid Conditions |
spellingShingle |
Leptospira spp. of the Urinary Tract of Female Carrier Goats in Semi-Arid Conditions Rocha, Laysa Mayara Soares Brito Leptospirosis Leptospires Zoonosis Serology Molecular detection Epidemiology semiarid |
title_short |
Leptospira spp. of the Urinary Tract of Female Carrier Goats in Semi-Arid Conditions |
title_full |
Leptospira spp. of the Urinary Tract of Female Carrier Goats in Semi-Arid Conditions |
title_fullStr |
Leptospira spp. of the Urinary Tract of Female Carrier Goats in Semi-Arid Conditions |
title_full_unstemmed |
Leptospira spp. of the Urinary Tract of Female Carrier Goats in Semi-Arid Conditions |
title_sort |
Leptospira spp. of the Urinary Tract of Female Carrier Goats in Semi-Arid Conditions |
author |
Rocha, Laysa Mayara Soares Brito |
author_facet |
Rocha, Laysa Mayara Soares Brito Faria, Pedro Jorge Alvares de Soares, Rafael Rodrigues Araujo Junior, Joao Pessoa [UNESP] Malossi, Camila Dantas [UNESP] Ullmann, Leila Sabrina [UNESP] Silva, Maria Luana Cristiny Rodrigues Higino, Severino Silvano dos Santos Azevedo, Sergio Santos de Alves, Clebert Jose |
author_role |
author |
author2 |
Faria, Pedro Jorge Alvares de Soares, Rafael Rodrigues Araujo Junior, Joao Pessoa [UNESP] Malossi, Camila Dantas [UNESP] Ullmann, Leila Sabrina [UNESP] Silva, Maria Luana Cristiny Rodrigues Higino, Severino Silvano dos Santos Azevedo, Sergio Santos de Alves, Clebert Jose |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Fed Univ Campina Grande UFCG Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Rocha, Laysa Mayara Soares Brito Faria, Pedro Jorge Alvares de Soares, Rafael Rodrigues Araujo Junior, Joao Pessoa [UNESP] Malossi, Camila Dantas [UNESP] Ullmann, Leila Sabrina [UNESP] Silva, Maria Luana Cristiny Rodrigues Higino, Severino Silvano dos Santos Azevedo, Sergio Santos de Alves, Clebert Jose |
dc.subject.por.fl_str_mv |
Leptospirosis Leptospires Zoonosis Serology Molecular detection Epidemiology semiarid |
topic |
Leptospirosis Leptospires Zoonosis Serology Molecular detection Epidemiology semiarid |
description |
Background: Leptospirosis is an important infectious disease in goat farming, with a worldwide distribution. It is usually transmitted by rodents and the genital route, may cause reproductive losses, negatively impacting goat fanning. The diagnosis lies on serological, molecular and isolation techniques. Considering the importance of this disease for small ruminants, this work aimed to evaluate the serological, molecular findings and isolation of pathogenic leptospires in the urinary tract (kidney and bladder tissues) of goats. Materials, Methods & Results: Thirty-four adult goats were used for slaughter. Renal samples (n = 34), bladder (n = 34), were collected for isolation of the agent and molecular detection of Leptospira sp. and blood samples (n = 34) for serological testing. The polymerase chain reaction (PCR) was used as a molecular test and the microscopic serum agglutination test (MAT) was used as a serological test. Samples with DNA amplification were subjected to genetic sequencing. The presence of Leptospira DNA was found in the tissues of 8 (23.4%) goats, and of these, only 2 were positive in PCR and MAL There was a slight agreement between the PCR and MAT techniques (k = 0.150; P = 0.436). In 6 (17.6%) samples of renal tissue and 2 (5.8%) bladder samples, Leptospira DNA was detected. The genes in a kidney tissue sample were sequenced and demonstrated 99% similarity to Leptospira interrogans. Anti-Leptospira sp. were detected in 6 (17.6%) of the animals tested. Discussion: Serology identified 3 predominant serogroups: Icterohaemorrhagiae, Tarassovi and Autumnalis, serogroups that are related to the presence of rodents that coexist in rural environments. Autumnalis has been reported in small ruminants, raising the hypothesis that goats are adapted, becoming chronic carriers and possible maintenance hosts. The frequency obtained (17.6%) may be the result of the mixed breed pattern and rustic characteristics inherent to the goat species. Given the characteristics of the semi-arid region, such as low rainfall and high solar incidence, it is essential to use an adapted methodology, with a lower cut-off point (1:50), as the serological titer is an established relationship between the animal species, the level of exposure throughout its evolution and the region studied. Molecular findings and bacterial isolation reveal the agent's ability to colonize the urinary tract of goats. These data show the importance that urine has in the epidemiological chain, being able to transmit the agent through direct contact with this product or through contamination of soil and water. There was no statistical agreement between the diagnostic techniques used in this study, in this case, an association between PCR and MAT is recommended to obtain data with high sensitivity and specificity. A bladder sample was sequenced and showed 99% similarity to Leptospira interrogans. In the semiarid region, the most common form of leptospirosis spread is through the sale of animals in business fairs for breeding, rearing or slaughter, as well as sharing the same property with several breeders. The introduction of chronic and asymptomatic carriers on the properties represents a serious risk for the spread of the disease. The results show the presence of Leptospira spp. in semi-arid goat herds, having as risk factors the presence of rodents and intercropping. The association of MAT and PCR is necessary for a better diagnosis of the disease. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-30T13:38:57Z 2022-11-30T13:38:57Z 2022-06-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.22456/1679-9216.124079 Acta Scientiae Veterinariae. Porto Alegre Rs: Univ Fed Rio Grande Do Sul, v. 50, 8 p., 2022. 1678-0345 http://hdl.handle.net/11449/237573 10.22456/1679-9216.124079 WOS:000804934900001 |
url |
http://dx.doi.org/10.22456/1679-9216.124079 http://hdl.handle.net/11449/237573 |
identifier_str_mv |
Acta Scientiae Veterinariae. Porto Alegre Rs: Univ Fed Rio Grande Do Sul, v. 50, 8 p., 2022. 1678-0345 10.22456/1679-9216.124079 WOS:000804934900001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Scientiae Veterinariae |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 |
dc.publisher.none.fl_str_mv |
Univ Fed Rio Grande Do Sul |
publisher.none.fl_str_mv |
Univ Fed Rio Grande Do Sul |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045833810116608 |