Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10753-014-0001-4 http://hdl.handle.net/11449/206484 |
Resumo: | The myeloid differentiation factor 88 (MyD88) plays a pivotal role in Toll-like receptor (TLR)- and interleukin-1 receptor (IL-1R)-induced osteoclastogenesis. We examined the role of MyD88 on p38 mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) activation and nucleotide-binding oligomerization domain (Nod) induction by lipopolysaccharide (LPS) and IL-1 beta, and their effect on receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) production in bone marrow stromal cell (BMSC). RANKL, Nod1, Nod2, NF-κB, and p38 protein levels were determined by Western blot. Nod2 was stimulated with muramyl dipeptide (MDP) prior to TLR4 stimulation with LPS. MyD88 deficiency markedly inhibited RANKL expression after LPS stimulation and increased OPG messenger RNA (mRNA) production. Also, MyD88 was necessary for NF-κB and p38 MAPK activation. MDP alone did not induce RANKL and OPG expressions; however, when combined with LPS, their expressions were significantly increased (p < 0.05). Our results support that MyD88 signaling has a pivotal role in osteoclastogenesis thought NF-κB and p38 activation. Nod2 and especially Nod1 levels were influenced by MyD88. |
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Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cellslipopolysaccharidemitogen-activated protein kinasesnuclear factor kappa-light-chain-enhancer of activated B cellsosteoprotegerinreceptor activator for nuclear factor κ B ligandsignaling pathwayThe myeloid differentiation factor 88 (MyD88) plays a pivotal role in Toll-like receptor (TLR)- and interleukin-1 receptor (IL-1R)-induced osteoclastogenesis. We examined the role of MyD88 on p38 mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) activation and nucleotide-binding oligomerization domain (Nod) induction by lipopolysaccharide (LPS) and IL-1 beta, and their effect on receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) production in bone marrow stromal cell (BMSC). RANKL, Nod1, Nod2, NF-κB, and p38 protein levels were determined by Western blot. Nod2 was stimulated with muramyl dipeptide (MDP) prior to TLR4 stimulation with LPS. MyD88 deficiency markedly inhibited RANKL expression after LPS stimulation and increased OPG messenger RNA (mRNA) production. Also, MyD88 was necessary for NF-κB and p38 MAPK activation. MDP alone did not induce RANKL and OPG expressions; however, when combined with LPS, their expressions were significantly increased (p < 0.05). Our results support that MyD88 signaling has a pivotal role in osteoclastogenesis thought NF-κB and p38 activation. Nod2 and especially Nod1 levels were influenced by MyD88.School of Dentistry Federal University at Pelotas (UFPel)Department of Diagnosis and Surgery School of Dentistry at Araraquara State University of Sao Paulo (UNESP), Rua Humaitá, 1680, CentroDepartment of Cell Biology and Microbial Pathogenesis School of Medicine USPDepartment of Biochemistry and Immunology School of Medicine USPDepartment of Diagnosis and Surgery School of Dentistry at Araraquara State University of Sao Paulo (UNESP), Rua Humaitá, 1680, CentroUniversidade Federal de Pernambuco (UFPE)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Leite, Fábio Renato Manzolli [UNESP]de Aquino, Sabrina Garcia [UNESP]Guimarães, Morgana Rodrigues [UNESP]Cirelli, Joni Augusto [UNESP]Zamboni, Dario S.Silva, João S.Junior, Carlos Rossa [UNESP]2021-06-25T10:32:46Z2021-06-25T10:32:46Z2015-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10753-014-0001-4Inflammation, v. 38, n. 1, 2015.1573-25760360-3997http://hdl.handle.net/11449/20648410.1007/s10753-014-0001-42-s2.0-84939882277Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInflammationinfo:eu-repo/semantics/openAccess2024-09-26T15:21:38Zoai:repositorio.unesp.br:11449/206484Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T15:21:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells |
title |
Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells |
spellingShingle |
Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells Leite, Fábio Renato Manzolli [UNESP] lipopolysaccharide mitogen-activated protein kinases nuclear factor kappa-light-chain-enhancer of activated B cells osteoprotegerin receptor activator for nuclear factor κ B ligand signaling pathway |
title_short |
Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells |
title_full |
Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells |
title_fullStr |
Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells |
title_full_unstemmed |
Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells |
title_sort |
Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells |
author |
Leite, Fábio Renato Manzolli [UNESP] |
author_facet |
Leite, Fábio Renato Manzolli [UNESP] de Aquino, Sabrina Garcia [UNESP] Guimarães, Morgana Rodrigues [UNESP] Cirelli, Joni Augusto [UNESP] Zamboni, Dario S. Silva, João S. Junior, Carlos Rossa [UNESP] |
author_role |
author |
author2 |
de Aquino, Sabrina Garcia [UNESP] Guimarães, Morgana Rodrigues [UNESP] Cirelli, Joni Augusto [UNESP] Zamboni, Dario S. Silva, João S. Junior, Carlos Rossa [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Pernambuco (UFPE) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Leite, Fábio Renato Manzolli [UNESP] de Aquino, Sabrina Garcia [UNESP] Guimarães, Morgana Rodrigues [UNESP] Cirelli, Joni Augusto [UNESP] Zamboni, Dario S. Silva, João S. Junior, Carlos Rossa [UNESP] |
dc.subject.por.fl_str_mv |
lipopolysaccharide mitogen-activated protein kinases nuclear factor kappa-light-chain-enhancer of activated B cells osteoprotegerin receptor activator for nuclear factor κ B ligand signaling pathway |
topic |
lipopolysaccharide mitogen-activated protein kinases nuclear factor kappa-light-chain-enhancer of activated B cells osteoprotegerin receptor activator for nuclear factor κ B ligand signaling pathway |
description |
The myeloid differentiation factor 88 (MyD88) plays a pivotal role in Toll-like receptor (TLR)- and interleukin-1 receptor (IL-1R)-induced osteoclastogenesis. We examined the role of MyD88 on p38 mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) activation and nucleotide-binding oligomerization domain (Nod) induction by lipopolysaccharide (LPS) and IL-1 beta, and their effect on receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) production in bone marrow stromal cell (BMSC). RANKL, Nod1, Nod2, NF-κB, and p38 protein levels were determined by Western blot. Nod2 was stimulated with muramyl dipeptide (MDP) prior to TLR4 stimulation with LPS. MyD88 deficiency markedly inhibited RANKL expression after LPS stimulation and increased OPG messenger RNA (mRNA) production. Also, MyD88 was necessary for NF-κB and p38 MAPK activation. MDP alone did not induce RANKL and OPG expressions; however, when combined with LPS, their expressions were significantly increased (p < 0.05). Our results support that MyD88 signaling has a pivotal role in osteoclastogenesis thought NF-κB and p38 activation. Nod2 and especially Nod1 levels were influenced by MyD88. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-02-01 2021-06-25T10:32:46Z 2021-06-25T10:32:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10753-014-0001-4 Inflammation, v. 38, n. 1, 2015. 1573-2576 0360-3997 http://hdl.handle.net/11449/206484 10.1007/s10753-014-0001-4 2-s2.0-84939882277 |
url |
http://dx.doi.org/10.1007/s10753-014-0001-4 http://hdl.handle.net/11449/206484 |
identifier_str_mv |
Inflammation, v. 38, n. 1, 2015. 1573-2576 0360-3997 10.1007/s10753-014-0001-4 2-s2.0-84939882277 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Inflammation |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546452457095168 |