Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells

Detalhes bibliográficos
Autor(a) principal: Leite, Fábio Renato Manzolli [UNESP]
Data de Publicação: 2015
Outros Autores: de Aquino, Sabrina Garcia [UNESP], Guimarães, Morgana Rodrigues [UNESP], Cirelli, Joni Augusto [UNESP], Zamboni, Dario S., Silva, João S., Junior, Carlos Rossa [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10753-014-0001-4
http://hdl.handle.net/11449/206484
Resumo: The myeloid differentiation factor 88 (MyD88) plays a pivotal role in Toll-like receptor (TLR)- and interleukin-1 receptor (IL-1R)-induced osteoclastogenesis. We examined the role of MyD88 on p38 mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) activation and nucleotide-binding oligomerization domain (Nod) induction by lipopolysaccharide (LPS) and IL-1 beta, and their effect on receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) production in bone marrow stromal cell (BMSC). RANKL, Nod1, Nod2, NF-κB, and p38 protein levels were determined by Western blot. Nod2 was stimulated with muramyl dipeptide (MDP) prior to TLR4 stimulation with LPS. MyD88 deficiency markedly inhibited RANKL expression after LPS stimulation and increased OPG messenger RNA (mRNA) production. Also, MyD88 was necessary for NF-κB and p38 MAPK activation. MDP alone did not induce RANKL and OPG expressions; however, when combined with LPS, their expressions were significantly increased (p < 0.05). Our results support that MyD88 signaling has a pivotal role in osteoclastogenesis thought NF-κB and p38 activation. Nod2 and especially Nod1 levels were influenced by MyD88.
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spelling Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cellslipopolysaccharidemitogen-activated protein kinasesnuclear factor kappa-light-chain-enhancer of activated B cellsosteoprotegerinreceptor activator for nuclear factor κ B ligandsignaling pathwayThe myeloid differentiation factor 88 (MyD88) plays a pivotal role in Toll-like receptor (TLR)- and interleukin-1 receptor (IL-1R)-induced osteoclastogenesis. We examined the role of MyD88 on p38 mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) activation and nucleotide-binding oligomerization domain (Nod) induction by lipopolysaccharide (LPS) and IL-1 beta, and their effect on receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) production in bone marrow stromal cell (BMSC). RANKL, Nod1, Nod2, NF-κB, and p38 protein levels were determined by Western blot. Nod2 was stimulated with muramyl dipeptide (MDP) prior to TLR4 stimulation with LPS. MyD88 deficiency markedly inhibited RANKL expression after LPS stimulation and increased OPG messenger RNA (mRNA) production. Also, MyD88 was necessary for NF-κB and p38 MAPK activation. MDP alone did not induce RANKL and OPG expressions; however, when combined with LPS, their expressions were significantly increased (p < 0.05). Our results support that MyD88 signaling has a pivotal role in osteoclastogenesis thought NF-κB and p38 activation. Nod2 and especially Nod1 levels were influenced by MyD88.School of Dentistry Federal University at Pelotas (UFPel)Department of Diagnosis and Surgery School of Dentistry at Araraquara State University of Sao Paulo (UNESP), Rua Humaitá, 1680, CentroDepartment of Cell Biology and Microbial Pathogenesis School of Medicine USPDepartment of Biochemistry and Immunology School of Medicine USPDepartment of Diagnosis and Surgery School of Dentistry at Araraquara State University of Sao Paulo (UNESP), Rua Humaitá, 1680, CentroUniversidade Federal de Pernambuco (UFPE)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Leite, Fábio Renato Manzolli [UNESP]de Aquino, Sabrina Garcia [UNESP]Guimarães, Morgana Rodrigues [UNESP]Cirelli, Joni Augusto [UNESP]Zamboni, Dario S.Silva, João S.Junior, Carlos Rossa [UNESP]2021-06-25T10:32:46Z2021-06-25T10:32:46Z2015-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10753-014-0001-4Inflammation, v. 38, n. 1, 2015.1573-25760360-3997http://hdl.handle.net/11449/20648410.1007/s10753-014-0001-42-s2.0-84939882277Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInflammationinfo:eu-repo/semantics/openAccess2024-09-26T15:21:38Zoai:repositorio.unesp.br:11449/206484Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T15:21:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells
title Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells
spellingShingle Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells
Leite, Fábio Renato Manzolli [UNESP]
lipopolysaccharide
mitogen-activated protein kinases
nuclear factor kappa-light-chain-enhancer of activated B cells
osteoprotegerin
receptor activator for nuclear factor κ B ligand
signaling pathway
title_short Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells
title_full Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells
title_fullStr Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells
title_full_unstemmed Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells
title_sort Relevance of the Myeloid Differentiation Factor 88 (MyD88) on RANKL, OPG, and Nod Expressions Induced by TLR and IL-1R Signaling in Bone Marrow Stromal Cells
author Leite, Fábio Renato Manzolli [UNESP]
author_facet Leite, Fábio Renato Manzolli [UNESP]
de Aquino, Sabrina Garcia [UNESP]
Guimarães, Morgana Rodrigues [UNESP]
Cirelli, Joni Augusto [UNESP]
Zamboni, Dario S.
Silva, João S.
Junior, Carlos Rossa [UNESP]
author_role author
author2 de Aquino, Sabrina Garcia [UNESP]
Guimarães, Morgana Rodrigues [UNESP]
Cirelli, Joni Augusto [UNESP]
Zamboni, Dario S.
Silva, João S.
Junior, Carlos Rossa [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Pernambuco (UFPE)
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Leite, Fábio Renato Manzolli [UNESP]
de Aquino, Sabrina Garcia [UNESP]
Guimarães, Morgana Rodrigues [UNESP]
Cirelli, Joni Augusto [UNESP]
Zamboni, Dario S.
Silva, João S.
Junior, Carlos Rossa [UNESP]
dc.subject.por.fl_str_mv lipopolysaccharide
mitogen-activated protein kinases
nuclear factor kappa-light-chain-enhancer of activated B cells
osteoprotegerin
receptor activator for nuclear factor κ B ligand
signaling pathway
topic lipopolysaccharide
mitogen-activated protein kinases
nuclear factor kappa-light-chain-enhancer of activated B cells
osteoprotegerin
receptor activator for nuclear factor κ B ligand
signaling pathway
description The myeloid differentiation factor 88 (MyD88) plays a pivotal role in Toll-like receptor (TLR)- and interleukin-1 receptor (IL-1R)-induced osteoclastogenesis. We examined the role of MyD88 on p38 mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) activation and nucleotide-binding oligomerization domain (Nod) induction by lipopolysaccharide (LPS) and IL-1 beta, and their effect on receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) production in bone marrow stromal cell (BMSC). RANKL, Nod1, Nod2, NF-κB, and p38 protein levels were determined by Western blot. Nod2 was stimulated with muramyl dipeptide (MDP) prior to TLR4 stimulation with LPS. MyD88 deficiency markedly inhibited RANKL expression after LPS stimulation and increased OPG messenger RNA (mRNA) production. Also, MyD88 was necessary for NF-κB and p38 MAPK activation. MDP alone did not induce RANKL and OPG expressions; however, when combined with LPS, their expressions were significantly increased (p < 0.05). Our results support that MyD88 signaling has a pivotal role in osteoclastogenesis thought NF-κB and p38 activation. Nod2 and especially Nod1 levels were influenced by MyD88.
publishDate 2015
dc.date.none.fl_str_mv 2015-02-01
2021-06-25T10:32:46Z
2021-06-25T10:32:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10753-014-0001-4
Inflammation, v. 38, n. 1, 2015.
1573-2576
0360-3997
http://hdl.handle.net/11449/206484
10.1007/s10753-014-0001-4
2-s2.0-84939882277
url http://dx.doi.org/10.1007/s10753-014-0001-4
http://hdl.handle.net/11449/206484
identifier_str_mv Inflammation, v. 38, n. 1, 2015.
1573-2576
0360-3997
10.1007/s10753-014-0001-4
2-s2.0-84939882277
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Inflammation
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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