In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1519-6984.205893 http://hdl.handle.net/11449/200666 |
Resumo: | Although the potential of surrogate propagation technology for aquaculture and conservation of Neotropical fish, the poor understanding of the host immune system may results in rejection and destruction of the donor material. Thus, it is necessary to study and to develop methods to evaluate the effects of immunosuppressive drugs employment and to evaluate the immunocompatibility between donor and receptor. Thus, the present study aimed to optimize a methodology to assess in vivo phagocytosis in Astyanax altiparanae using Saccharomyces cerevisiae and to evaluate their hematological response resultant from the inflammatory induction. To this, S. cerevisiae were labeled with Congo red and injected in the coelomic cavity of A. altiparanae at the concentration of 2.5 x 106 cells mL-1. A PBS solution and a non-injected group were kept as control. Fish blood was sampled and the phagocytic capacity and index were determined at 1, 2, 3 and 6 h post-injection (hpi). The yeast injection successfully stimulated phagocytosis, with the best result for phagocytosis assessment after 2 hpi. Moreover, it was achieved a high traceability of phagocytized and non-phagocytized yeast under optic microscopy analysis due to the Congo red labeling. The hematological profile was similar to usually observed in early infections, indicating lymphocyte migration to inflammatory site and increase in number of circulating phagocytes due to natural response to inflammatory stimulus. In conclusion, our method was efficient to assess in vivo phagocytosis in A. altiparanae and will be an important tool to evaluate the efficacy of immunosuppressive drugs in this species. Additionally, these results may serve as support for further studies in fish immunocompetence, both in laboratory and in field conditions. |
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In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technologyFagocitose in vivo e hematologia em astyanax altiparanae, um potencial modelo para tecnologia de propagação mediadaHematologyInnate immune responseNeotropical fishSurrogate technology hostAlthough the potential of surrogate propagation technology for aquaculture and conservation of Neotropical fish, the poor understanding of the host immune system may results in rejection and destruction of the donor material. Thus, it is necessary to study and to develop methods to evaluate the effects of immunosuppressive drugs employment and to evaluate the immunocompatibility between donor and receptor. Thus, the present study aimed to optimize a methodology to assess in vivo phagocytosis in Astyanax altiparanae using Saccharomyces cerevisiae and to evaluate their hematological response resultant from the inflammatory induction. To this, S. cerevisiae were labeled with Congo red and injected in the coelomic cavity of A. altiparanae at the concentration of 2.5 x 106 cells mL-1. A PBS solution and a non-injected group were kept as control. Fish blood was sampled and the phagocytic capacity and index were determined at 1, 2, 3 and 6 h post-injection (hpi). The yeast injection successfully stimulated phagocytosis, with the best result for phagocytosis assessment after 2 hpi. Moreover, it was achieved a high traceability of phagocytized and non-phagocytized yeast under optic microscopy analysis due to the Congo red labeling. The hematological profile was similar to usually observed in early infections, indicating lymphocyte migration to inflammatory site and increase in number of circulating phagocytes due to natural response to inflammatory stimulus. In conclusion, our method was efficient to assess in vivo phagocytosis in A. altiparanae and will be an important tool to evaluate the efficacy of immunosuppressive drugs in this species. Additionally, these results may serve as support for further studies in fish immunocompetence, both in laboratory and in field conditions.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Instituto Chico Mendes de Conservação da BiodiversidadeLaboratório de Microbiologia e Parasitologia de Organismos Aquáticos LAPOA Centro de Aquicultura da UNESP – CAUNESP Universidade Estadual Paulista – UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/nLaboratório de Biotecnologia de Peixes Instituto Chico Mendes de Conservação da Biodiversidade – ICMBio, Rodovia Pref. Euberto Nemésio Pereira de GodoyInstituto de Biociências – IBB Universidade Estadual Paulista – UNESP, Rua Prof. Doutor Antonio Celso Wagner Zanin, s/nDepartamento de Medicina Veterinária Universidade de São Paulo – USP, Av. Duque de Caxias Norte, 225, Zona RuralInstituto de Saúde e Estudos Biológicos – IESB Universidade Federal do Sul e Sudeste do Pará – UNIFESSPA, Folha 31, Quadra 7, Lote Especial, s/nLaboratório de Microbiologia e Parasitologia de Organismos Aquáticos LAPOA Centro de Aquicultura da UNESP – CAUNESP Universidade Estadual Paulista – UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/nInstituto de Biociências – IBB Universidade Estadual Paulista – UNESP, Rua Prof. Doutor Antonio Celso Wagner Zanin, s/nCAPES: 1.186.792Instituto Chico Mendes de Conservação da Biodiversidade: 4690000174Universidade Estadual Paulista (Unesp)Instituto Chico Mendes de Conservação da Biodiversidade – ICMBioUniversidade de São Paulo (USP)Universidade Federal do Sul e Sudeste do Pará – UNIFESSPALevy-Pereira, N. [UNESP]Yasui, G. S. [UNESP]Evangelista, M. M. [UNESP]Nascimento, N. F. [UNESP]Santos, M. P. [UNESP]Siqueira-Silva, D. H.Monzani, P. S.Senhorini, J. A. [UNESP]Pilarski, F. [UNESP]2020-12-12T02:12:49Z2020-12-12T02:12:49Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article336-344application/pdfhttp://dx.doi.org/10.1590/1519-6984.205893Brazilian Journal of Biology, v. 80, n. 2, p. 336-344, 2020.1678-43751519-6984http://hdl.handle.net/11449/20066610.1590/1519-6984.205893S1519-698420200002003362-s2.0-85087111379S1519-69842020000200336.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Biologyinfo:eu-repo/semantics/openAccess2024-04-09T15:43:44Zoai:repositorio.unesp.br:11449/200666Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:58:03.161551Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology Fagocitose in vivo e hematologia em astyanax altiparanae, um potencial modelo para tecnologia de propagação mediada |
title |
In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology |
spellingShingle |
In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology Levy-Pereira, N. [UNESP] Hematology Innate immune response Neotropical fish Surrogate technology host |
title_short |
In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology |
title_full |
In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology |
title_fullStr |
In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology |
title_full_unstemmed |
In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology |
title_sort |
In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology |
author |
Levy-Pereira, N. [UNESP] |
author_facet |
Levy-Pereira, N. [UNESP] Yasui, G. S. [UNESP] Evangelista, M. M. [UNESP] Nascimento, N. F. [UNESP] Santos, M. P. [UNESP] Siqueira-Silva, D. H. Monzani, P. S. Senhorini, J. A. [UNESP] Pilarski, F. [UNESP] |
author_role |
author |
author2 |
Yasui, G. S. [UNESP] Evangelista, M. M. [UNESP] Nascimento, N. F. [UNESP] Santos, M. P. [UNESP] Siqueira-Silva, D. H. Monzani, P. S. Senhorini, J. A. [UNESP] Pilarski, F. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Instituto Chico Mendes de Conservação da Biodiversidade – ICMBio Universidade de São Paulo (USP) Universidade Federal do Sul e Sudeste do Pará – UNIFESSPA |
dc.contributor.author.fl_str_mv |
Levy-Pereira, N. [UNESP] Yasui, G. S. [UNESP] Evangelista, M. M. [UNESP] Nascimento, N. F. [UNESP] Santos, M. P. [UNESP] Siqueira-Silva, D. H. Monzani, P. S. Senhorini, J. A. [UNESP] Pilarski, F. [UNESP] |
dc.subject.por.fl_str_mv |
Hematology Innate immune response Neotropical fish Surrogate technology host |
topic |
Hematology Innate immune response Neotropical fish Surrogate technology host |
description |
Although the potential of surrogate propagation technology for aquaculture and conservation of Neotropical fish, the poor understanding of the host immune system may results in rejection and destruction of the donor material. Thus, it is necessary to study and to develop methods to evaluate the effects of immunosuppressive drugs employment and to evaluate the immunocompatibility between donor and receptor. Thus, the present study aimed to optimize a methodology to assess in vivo phagocytosis in Astyanax altiparanae using Saccharomyces cerevisiae and to evaluate their hematological response resultant from the inflammatory induction. To this, S. cerevisiae were labeled with Congo red and injected in the coelomic cavity of A. altiparanae at the concentration of 2.5 x 106 cells mL-1. A PBS solution and a non-injected group were kept as control. Fish blood was sampled and the phagocytic capacity and index were determined at 1, 2, 3 and 6 h post-injection (hpi). The yeast injection successfully stimulated phagocytosis, with the best result for phagocytosis assessment after 2 hpi. Moreover, it was achieved a high traceability of phagocytized and non-phagocytized yeast under optic microscopy analysis due to the Congo red labeling. The hematological profile was similar to usually observed in early infections, indicating lymphocyte migration to inflammatory site and increase in number of circulating phagocytes due to natural response to inflammatory stimulus. In conclusion, our method was efficient to assess in vivo phagocytosis in A. altiparanae and will be an important tool to evaluate the efficacy of immunosuppressive drugs in this species. Additionally, these results may serve as support for further studies in fish immunocompetence, both in laboratory and in field conditions. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:12:49Z 2020-12-12T02:12:49Z 2020-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1519-6984.205893 Brazilian Journal of Biology, v. 80, n. 2, p. 336-344, 2020. 1678-4375 1519-6984 http://hdl.handle.net/11449/200666 10.1590/1519-6984.205893 S1519-69842020000200336 2-s2.0-85087111379 S1519-69842020000200336.pdf |
url |
http://dx.doi.org/10.1590/1519-6984.205893 http://hdl.handle.net/11449/200666 |
identifier_str_mv |
Brazilian Journal of Biology, v. 80, n. 2, p. 336-344, 2020. 1678-4375 1519-6984 10.1590/1519-6984.205893 S1519-69842020000200336 2-s2.0-85087111379 S1519-69842020000200336.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
336-344 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129567521505280 |