In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology

Detalhes bibliográficos
Autor(a) principal: Levy-Pereira, N. [UNESP]
Data de Publicação: 2020
Outros Autores: Yasui, G. S. [UNESP], Evangelista, M. M. [UNESP], Nascimento, N. F. [UNESP], Santos, M. P. [UNESP], Siqueira-Silva, D. H., Monzani, P. S., Senhorini, J. A. [UNESP], Pilarski, F. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1519-6984.205893
http://hdl.handle.net/11449/200666
Resumo: Although the potential of surrogate propagation technology for aquaculture and conservation of Neotropical fish, the poor understanding of the host immune system may results in rejection and destruction of the donor material. Thus, it is necessary to study and to develop methods to evaluate the effects of immunosuppressive drugs employment and to evaluate the immunocompatibility between donor and receptor. Thus, the present study aimed to optimize a methodology to assess in vivo phagocytosis in Astyanax altiparanae using Saccharomyces cerevisiae and to evaluate their hematological response resultant from the inflammatory induction. To this, S. cerevisiae were labeled with Congo red and injected in the coelomic cavity of A. altiparanae at the concentration of 2.5 x 106 cells mL-1. A PBS solution and a non-injected group were kept as control. Fish blood was sampled and the phagocytic capacity and index were determined at 1, 2, 3 and 6 h post-injection (hpi). The yeast injection successfully stimulated phagocytosis, with the best result for phagocytosis assessment after 2 hpi. Moreover, it was achieved a high traceability of phagocytized and non-phagocytized yeast under optic microscopy analysis due to the Congo red labeling. The hematological profile was similar to usually observed in early infections, indicating lymphocyte migration to inflammatory site and increase in number of circulating phagocytes due to natural response to inflammatory stimulus. In conclusion, our method was efficient to assess in vivo phagocytosis in A. altiparanae and will be an important tool to evaluate the efficacy of immunosuppressive drugs in this species. Additionally, these results may serve as support for further studies in fish immunocompetence, both in laboratory and in field conditions.
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spelling In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technologyFagocitose in vivo e hematologia em astyanax altiparanae, um potencial modelo para tecnologia de propagação mediadaHematologyInnate immune responseNeotropical fishSurrogate technology hostAlthough the potential of surrogate propagation technology for aquaculture and conservation of Neotropical fish, the poor understanding of the host immune system may results in rejection and destruction of the donor material. Thus, it is necessary to study and to develop methods to evaluate the effects of immunosuppressive drugs employment and to evaluate the immunocompatibility between donor and receptor. Thus, the present study aimed to optimize a methodology to assess in vivo phagocytosis in Astyanax altiparanae using Saccharomyces cerevisiae and to evaluate their hematological response resultant from the inflammatory induction. To this, S. cerevisiae were labeled with Congo red and injected in the coelomic cavity of A. altiparanae at the concentration of 2.5 x 106 cells mL-1. A PBS solution and a non-injected group were kept as control. Fish blood was sampled and the phagocytic capacity and index were determined at 1, 2, 3 and 6 h post-injection (hpi). The yeast injection successfully stimulated phagocytosis, with the best result for phagocytosis assessment after 2 hpi. Moreover, it was achieved a high traceability of phagocytized and non-phagocytized yeast under optic microscopy analysis due to the Congo red labeling. The hematological profile was similar to usually observed in early infections, indicating lymphocyte migration to inflammatory site and increase in number of circulating phagocytes due to natural response to inflammatory stimulus. In conclusion, our method was efficient to assess in vivo phagocytosis in A. altiparanae and will be an important tool to evaluate the efficacy of immunosuppressive drugs in this species. Additionally, these results may serve as support for further studies in fish immunocompetence, both in laboratory and in field conditions.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Instituto Chico Mendes de Conservação da BiodiversidadeLaboratório de Microbiologia e Parasitologia de Organismos Aquáticos LAPOA Centro de Aquicultura da UNESP – CAUNESP Universidade Estadual Paulista – UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/nLaboratório de Biotecnologia de Peixes Instituto Chico Mendes de Conservação da Biodiversidade – ICMBio, Rodovia Pref. Euberto Nemésio Pereira de GodoyInstituto de Biociências – IBB Universidade Estadual Paulista – UNESP, Rua Prof. Doutor Antonio Celso Wagner Zanin, s/nDepartamento de Medicina Veterinária Universidade de São Paulo – USP, Av. Duque de Caxias Norte, 225, Zona RuralInstituto de Saúde e Estudos Biológicos – IESB Universidade Federal do Sul e Sudeste do Pará – UNIFESSPA, Folha 31, Quadra 7, Lote Especial, s/nLaboratório de Microbiologia e Parasitologia de Organismos Aquáticos LAPOA Centro de Aquicultura da UNESP – CAUNESP Universidade Estadual Paulista – UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/nInstituto de Biociências – IBB Universidade Estadual Paulista – UNESP, Rua Prof. Doutor Antonio Celso Wagner Zanin, s/nCAPES: 1.186.792Instituto Chico Mendes de Conservação da Biodiversidade: 4690000174Universidade Estadual Paulista (Unesp)Instituto Chico Mendes de Conservação da Biodiversidade – ICMBioUniversidade de São Paulo (USP)Universidade Federal do Sul e Sudeste do Pará – UNIFESSPALevy-Pereira, N. [UNESP]Yasui, G. S. [UNESP]Evangelista, M. M. [UNESP]Nascimento, N. F. [UNESP]Santos, M. P. [UNESP]Siqueira-Silva, D. H.Monzani, P. S.Senhorini, J. A. [UNESP]Pilarski, F. [UNESP]2020-12-12T02:12:49Z2020-12-12T02:12:49Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article336-344application/pdfhttp://dx.doi.org/10.1590/1519-6984.205893Brazilian Journal of Biology, v. 80, n. 2, p. 336-344, 2020.1678-43751519-6984http://hdl.handle.net/11449/20066610.1590/1519-6984.205893S1519-698420200002003362-s2.0-85087111379S1519-69842020000200336.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Biologyinfo:eu-repo/semantics/openAccess2024-04-09T15:43:44Zoai:repositorio.unesp.br:11449/200666Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:58:03.161551Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology
Fagocitose in vivo e hematologia em astyanax altiparanae, um potencial modelo para tecnologia de propagação mediada
title In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology
spellingShingle In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology
Levy-Pereira, N. [UNESP]
Hematology
Innate immune response
Neotropical fish
Surrogate technology host
title_short In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology
title_full In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology
title_fullStr In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology
title_full_unstemmed In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology
title_sort In vivo phagocytosis and hematology in astyanax altiparanae, a potential model for surrogate technology
author Levy-Pereira, N. [UNESP]
author_facet Levy-Pereira, N. [UNESP]
Yasui, G. S. [UNESP]
Evangelista, M. M. [UNESP]
Nascimento, N. F. [UNESP]
Santos, M. P. [UNESP]
Siqueira-Silva, D. H.
Monzani, P. S.
Senhorini, J. A. [UNESP]
Pilarski, F. [UNESP]
author_role author
author2 Yasui, G. S. [UNESP]
Evangelista, M. M. [UNESP]
Nascimento, N. F. [UNESP]
Santos, M. P. [UNESP]
Siqueira-Silva, D. H.
Monzani, P. S.
Senhorini, J. A. [UNESP]
Pilarski, F. [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto Chico Mendes de Conservação da Biodiversidade – ICMBio
Universidade de São Paulo (USP)
Universidade Federal do Sul e Sudeste do Pará – UNIFESSPA
dc.contributor.author.fl_str_mv Levy-Pereira, N. [UNESP]
Yasui, G. S. [UNESP]
Evangelista, M. M. [UNESP]
Nascimento, N. F. [UNESP]
Santos, M. P. [UNESP]
Siqueira-Silva, D. H.
Monzani, P. S.
Senhorini, J. A. [UNESP]
Pilarski, F. [UNESP]
dc.subject.por.fl_str_mv Hematology
Innate immune response
Neotropical fish
Surrogate technology host
topic Hematology
Innate immune response
Neotropical fish
Surrogate technology host
description Although the potential of surrogate propagation technology for aquaculture and conservation of Neotropical fish, the poor understanding of the host immune system may results in rejection and destruction of the donor material. Thus, it is necessary to study and to develop methods to evaluate the effects of immunosuppressive drugs employment and to evaluate the immunocompatibility between donor and receptor. Thus, the present study aimed to optimize a methodology to assess in vivo phagocytosis in Astyanax altiparanae using Saccharomyces cerevisiae and to evaluate their hematological response resultant from the inflammatory induction. To this, S. cerevisiae were labeled with Congo red and injected in the coelomic cavity of A. altiparanae at the concentration of 2.5 x 106 cells mL-1. A PBS solution and a non-injected group were kept as control. Fish blood was sampled and the phagocytic capacity and index were determined at 1, 2, 3 and 6 h post-injection (hpi). The yeast injection successfully stimulated phagocytosis, with the best result for phagocytosis assessment after 2 hpi. Moreover, it was achieved a high traceability of phagocytized and non-phagocytized yeast under optic microscopy analysis due to the Congo red labeling. The hematological profile was similar to usually observed in early infections, indicating lymphocyte migration to inflammatory site and increase in number of circulating phagocytes due to natural response to inflammatory stimulus. In conclusion, our method was efficient to assess in vivo phagocytosis in A. altiparanae and will be an important tool to evaluate the efficacy of immunosuppressive drugs in this species. Additionally, these results may serve as support for further studies in fish immunocompetence, both in laboratory and in field conditions.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:12:49Z
2020-12-12T02:12:49Z
2020-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1519-6984.205893
Brazilian Journal of Biology, v. 80, n. 2, p. 336-344, 2020.
1678-4375
1519-6984
http://hdl.handle.net/11449/200666
10.1590/1519-6984.205893
S1519-69842020000200336
2-s2.0-85087111379
S1519-69842020000200336.pdf
url http://dx.doi.org/10.1590/1519-6984.205893
http://hdl.handle.net/11449/200666
identifier_str_mv Brazilian Journal of Biology, v. 80, n. 2, p. 336-344, 2020.
1678-4375
1519-6984
10.1590/1519-6984.205893
S1519-69842020000200336
2-s2.0-85087111379
S1519-69842020000200336.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 336-344
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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