Eficiência do protocolo superestimulatório P-36, associado à administração de eCG ou LH, em animais da raça Angus
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/91628 |
Resumo: | The protocol called P-36 has been broad/y used to induce multiple ovulations, due to the fact that it allows fixed-time artificial insemination (FTAI), and facilitates embryo donors handling. Recent studies with P-36 protocol, have shownthat replacement of the last two doses of FSH by eCG improves embryo yield. However, after consecutive use, eCG may induce antibody and decrease bovine embryo production. The objective of the present study is to verify the efficiency of protocol P-36/eCG in Angus breed, and to test the replacement of eCG by LH in the last day of superestimulatory treatment. In experiment 1, 22 Angus cows were randomly allotted to 4 groups: LH60 (Control), LH60/eCG, LH60/LH and LH60/FSH+LH. Each donor was superovulated 3 times, in such a way that each animal received 3 of 4 treatments, totaling 17 cows in the first two groups and 16 in the others (incomplete block design). At a random stage of the estrous cycle (DO), the embryo donors received an intravaginal device (lVO) containing 1.0 9 of progesterone and estradiol benzoate (3.0 mg, i.m.). In the control group the animais were superestimulated with pFSH (i.m., total dose = 200 mg) twice a day in decreasing doses from 04 to 7, whereas in groups LH60/eCG and LH60/LH the last two doses of FSH were replaced by eCG (i.m, total dose = 400 lU) or LH (i.m., total dose = 2.0 mg), respectively. Finally, the cows trom group LH60/FSH+LH received two doses of 1.0 mg of LH simultaneously with the last two doses of FSH. Ali animais were treated with dc1oprostenol (150 mg, i.m.) on day 6, and the IVD was removed 36 h after PGF2a administration. Ovulation was induced with 12.5 mg of pLH (i.m.), on day 8, and the animais were FTAI 12 and 24 h after pLH. In experiment 2, 17 cows were randomly allocated in 3 groups: LH48, LH60 and LH48/FSH+LH. The difference between the first and the second experiment is that in groups LH48 and LH48/FSH+LH ... (Complete abstract click electronic access below) |
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Eficiência do protocolo superestimulatório P-36, associado à administração de eCG ou LH, em animais da raça AngusBovino - Reprodução - Aspectos farmacológicos - Estudos experimentaisInseminação artificialTransferência de embriõesFarmacologia veterinariaBos taurusEmbryo transferSuperovulationThe protocol called P-36 has been broad/y used to induce multiple ovulations, due to the fact that it allows fixed-time artificial insemination (FTAI), and facilitates embryo donors handling. Recent studies with P-36 protocol, have shownthat replacement of the last two doses of FSH by eCG improves embryo yield. However, after consecutive use, eCG may induce antibody and decrease bovine embryo production. The objective of the present study is to verify the efficiency of protocol P-36/eCG in Angus breed, and to test the replacement of eCG by LH in the last day of superestimulatory treatment. In experiment 1, 22 Angus cows were randomly allotted to 4 groups: LH60 (Control), LH60/eCG, LH60/LH and LH60/FSH+LH. Each donor was superovulated 3 times, in such a way that each animal received 3 of 4 treatments, totaling 17 cows in the first two groups and 16 in the others (incomplete block design). At a random stage of the estrous cycle (DO), the embryo donors received an intravaginal device (lVO) containing 1.0 9 of progesterone and estradiol benzoate (3.0 mg, i.m.). In the control group the animais were superestimulated with pFSH (i.m., total dose = 200 mg) twice a day in decreasing doses from 04 to 7, whereas in groups LH60/eCG and LH60/LH the last two doses of FSH were replaced by eCG (i.m, total dose = 400 lU) or LH (i.m., total dose = 2.0 mg), respectively. Finally, the cows trom group LH60/FSH+LH received two doses of 1.0 mg of LH simultaneously with the last two doses of FSH. Ali animais were treated with dc1oprostenol (150 mg, i.m.) on day 6, and the IVD was removed 36 h after PGF2a administration. Ovulation was induced with 12.5 mg of pLH (i.m.), on day 8, and the animais were FTAI 12 and 24 h after pLH. In experiment 2, 17 cows were randomly allocated in 3 groups: LH48, LH60 and LH48/FSH+LH. The difference between the first and the second experiment is that in groups LH48 and LH48/FSH+LH ... (Complete abstract click electronic access below)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista (Unesp)Barros, Ciro Moraes [UNESP]Universidade Estadual Paulista (Unesp)Rosa, Fernanda de Souza [UNESP]2014-06-11T19:25:25Z2014-06-11T19:25:25Z2010-02-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis63 f. : il., tabs.application/pdfROSA, Fernanda de Souza. Eficiência do protocolo superestimulatório P-36, associado à administração de eCG ou LH, em animais da raça Angus. 2010. 63 f. Dissertação (mestrado) - Universidade Estadual Paulista, Instituto de Biociências de Botucatu, 2010.http://hdl.handle.net/11449/91628000610860rosa_fs_me_botib.pdf33004064052P07795883009987806Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-01-25T06:30:23Zoai:repositorio.unesp.br:11449/91628Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:54:42.807917Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Eficiência do protocolo superestimulatório P-36, associado à administração de eCG ou LH, em animais da raça Angus |
title |
Eficiência do protocolo superestimulatório P-36, associado à administração de eCG ou LH, em animais da raça Angus |
spellingShingle |
Eficiência do protocolo superestimulatório P-36, associado à administração de eCG ou LH, em animais da raça Angus Rosa, Fernanda de Souza [UNESP] Bovino - Reprodução - Aspectos farmacológicos - Estudos experimentais Inseminação artificial Transferência de embriões Farmacologia veterinaria Bos taurus Embryo transfer Superovulation |
title_short |
Eficiência do protocolo superestimulatório P-36, associado à administração de eCG ou LH, em animais da raça Angus |
title_full |
Eficiência do protocolo superestimulatório P-36, associado à administração de eCG ou LH, em animais da raça Angus |
title_fullStr |
Eficiência do protocolo superestimulatório P-36, associado à administração de eCG ou LH, em animais da raça Angus |
title_full_unstemmed |
Eficiência do protocolo superestimulatório P-36, associado à administração de eCG ou LH, em animais da raça Angus |
title_sort |
Eficiência do protocolo superestimulatório P-36, associado à administração de eCG ou LH, em animais da raça Angus |
author |
Rosa, Fernanda de Souza [UNESP] |
author_facet |
Rosa, Fernanda de Souza [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Barros, Ciro Moraes [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rosa, Fernanda de Souza [UNESP] |
dc.subject.por.fl_str_mv |
Bovino - Reprodução - Aspectos farmacológicos - Estudos experimentais Inseminação artificial Transferência de embriões Farmacologia veterinaria Bos taurus Embryo transfer Superovulation |
topic |
Bovino - Reprodução - Aspectos farmacológicos - Estudos experimentais Inseminação artificial Transferência de embriões Farmacologia veterinaria Bos taurus Embryo transfer Superovulation |
description |
The protocol called P-36 has been broad/y used to induce multiple ovulations, due to the fact that it allows fixed-time artificial insemination (FTAI), and facilitates embryo donors handling. Recent studies with P-36 protocol, have shownthat replacement of the last two doses of FSH by eCG improves embryo yield. However, after consecutive use, eCG may induce antibody and decrease bovine embryo production. The objective of the present study is to verify the efficiency of protocol P-36/eCG in Angus breed, and to test the replacement of eCG by LH in the last day of superestimulatory treatment. In experiment 1, 22 Angus cows were randomly allotted to 4 groups: LH60 (Control), LH60/eCG, LH60/LH and LH60/FSH+LH. Each donor was superovulated 3 times, in such a way that each animal received 3 of 4 treatments, totaling 17 cows in the first two groups and 16 in the others (incomplete block design). At a random stage of the estrous cycle (DO), the embryo donors received an intravaginal device (lVO) containing 1.0 9 of progesterone and estradiol benzoate (3.0 mg, i.m.). In the control group the animais were superestimulated with pFSH (i.m., total dose = 200 mg) twice a day in decreasing doses from 04 to 7, whereas in groups LH60/eCG and LH60/LH the last two doses of FSH were replaced by eCG (i.m, total dose = 400 lU) or LH (i.m., total dose = 2.0 mg), respectively. Finally, the cows trom group LH60/FSH+LH received two doses of 1.0 mg of LH simultaneously with the last two doses of FSH. Ali animais were treated with dc1oprostenol (150 mg, i.m.) on day 6, and the IVD was removed 36 h after PGF2a administration. Ovulation was induced with 12.5 mg of pLH (i.m.), on day 8, and the animais were FTAI 12 and 24 h after pLH. In experiment 2, 17 cows were randomly allocated in 3 groups: LH48, LH60 and LH48/FSH+LH. The difference between the first and the second experiment is that in groups LH48 and LH48/FSH+LH ... (Complete abstract click electronic access below) |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-02-25 2014-06-11T19:25:25Z 2014-06-11T19:25:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ROSA, Fernanda de Souza. Eficiência do protocolo superestimulatório P-36, associado à administração de eCG ou LH, em animais da raça Angus. 2010. 63 f. Dissertação (mestrado) - Universidade Estadual Paulista, Instituto de Biociências de Botucatu, 2010. http://hdl.handle.net/11449/91628 000610860 rosa_fs_me_botib.pdf 33004064052P0 7795883009987806 |
identifier_str_mv |
ROSA, Fernanda de Souza. Eficiência do protocolo superestimulatório P-36, associado à administração de eCG ou LH, em animais da raça Angus. 2010. 63 f. Dissertação (mestrado) - Universidade Estadual Paulista, Instituto de Biociências de Botucatu, 2010. 000610860 rosa_fs_me_botib.pdf 33004064052P0 7795883009987806 |
url |
http://hdl.handle.net/11449/91628 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
63 f. : il., tabs. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129563759214592 |