Uncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae)
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0150573 http://hdl.handle.net/11449/161297 |
Resumo: | B chromosomes constitute a heterogeneous mixture of genomic parasites that are sometimes derived intraspecifically from the standard genome of the host species, but result from interspecific hybridization in other cases. The mode of origin determines the DNA content, with the B chromosomes showing high similarity with the A genome in the first case, but presenting higher similarity with a different species in the second. The characid fish Moenkhausia sanctaefilomenae harbours highly invasive B chromosomes, which are present in all populations analyzed to date in the Parana and Tiete rivers. To investigate the origin of these B chromosomes, we analyzed two natural populations: one carrying B chromosomes and the other lacking them, using a combination of molecular cytogenetic techniques, nucleotide sequence analysis and high-throughput sequencing (Illumina HiSeq2000). Our results showed that i) B chromosomes have not yet reached the Paranapanema River basin; ii) B chromosomes are mitotically unstable; iii) there are two types of B chromosomes, the most frequent of which is lightly C-banded (similar to euchromatin in A chromosomes) (B-1), while the other is darkly C-banded (heterochromatin-like) (B-2); iv) the two B types contain the same tandem repeat DNA sequences (18S ribosomal DNA, H3 histone genes, MS3 and MS7 satellite DNA), with a higher content of 18S rDNA in the heterochromatic variant; v) all of these repetitive DNAs are present together only in the paracentromeric region of autosome pair no. 6, suggesting that the B chromosomes are derived from this A chromosome; vi) the two B chromosome variants show MS3 sequences that are highly divergent from each other and from the 0B genome, although the B-2-derived sequences exhibit higher similarity with the 0B genome (this suggests an independent origin of the two B variants, with the less frequent, B-2 type presumably being younger); and vii) the dN/dS ratio for the H3.2 histone gene is almost 4-6 times higher for B chromosomes than for A chromosome sequences, suggesting that purifying selection is relaxed for the DNA sequences located on the B chromosomes, presumably because they are mostly inactive. |
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Uncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae)B chromosomes constitute a heterogeneous mixture of genomic parasites that are sometimes derived intraspecifically from the standard genome of the host species, but result from interspecific hybridization in other cases. The mode of origin determines the DNA content, with the B chromosomes showing high similarity with the A genome in the first case, but presenting higher similarity with a different species in the second. The characid fish Moenkhausia sanctaefilomenae harbours highly invasive B chromosomes, which are present in all populations analyzed to date in the Parana and Tiete rivers. To investigate the origin of these B chromosomes, we analyzed two natural populations: one carrying B chromosomes and the other lacking them, using a combination of molecular cytogenetic techniques, nucleotide sequence analysis and high-throughput sequencing (Illumina HiSeq2000). Our results showed that i) B chromosomes have not yet reached the Paranapanema River basin; ii) B chromosomes are mitotically unstable; iii) there are two types of B chromosomes, the most frequent of which is lightly C-banded (similar to euchromatin in A chromosomes) (B-1), while the other is darkly C-banded (heterochromatin-like) (B-2); iv) the two B types contain the same tandem repeat DNA sequences (18S ribosomal DNA, H3 histone genes, MS3 and MS7 satellite DNA), with a higher content of 18S rDNA in the heterochromatic variant; v) all of these repetitive DNAs are present together only in the paracentromeric region of autosome pair no. 6, suggesting that the B chromosomes are derived from this A chromosome; vi) the two B chromosome variants show MS3 sequences that are highly divergent from each other and from the 0B genome, although the B-2-derived sequences exhibit higher similarity with the 0B genome (this suggests an independent origin of the two B variants, with the less frequent, B-2 type presumably being younger); and vii) the dN/dS ratio for the H3.2 histone gene is almost 4-6 times higher for B chromosomes than for A chromosome sequences, suggesting that purifying selection is relaxed for the DNA sequences located on the B chromosomes, presumably because they are mostly inactive.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, Inst Biociencias Botucatu, Dept Morfol, Botucatu, SP, BrazilUniv Granada, Dept Genet, Granada, SpainUniv Estadual Paulista, CAUNESP, Sao Paulo, BrazilInst Mol & Cellular Biol SB RAS, Novosibirsk, RussiaUniv Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, Sao Paulo, BrazilUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Morfol, Botucatu, SP, BrazilUniv Estadual Paulista, CAUNESP, Sao Paulo, BrazilUniv Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, Sao Paulo, BrazilFAPESP: 13/00953-8Public Library ScienceUniversidade Estadual Paulista (Unesp)Univ GranadaInst Mol & Cellular Biol SB RASUtsunomia, Ricardo [UNESP]Zerbinato de Andrade Silva, Duilio Mazzoni [UNESP]Ruiz-Ruano, Francisco J.Araya-Jaime, Cristian [UNESP]Pansonato-Alves, Jose Carlos [UNESP]Scacchetti, Priscilla Cardim [UNESP]Hashimoto, Diogo Teruo [UNESP]Oliveira, Claudio [UNESP]Trifonov, Vladmir A.Porto-Foresti, Fabio [UNESP]Camacho, Juan Pedro M.Foresti, Fausto [UNESP]2018-11-26T16:27:56Z2018-11-26T16:27:56Z2016-03-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20application/pdfhttp://dx.doi.org/10.1371/journal.pone.0150573Plos One. San Francisco: Public Library Science, v. 11, n. 3, 20 p., 2016.1932-6203http://hdl.handle.net/11449/16129710.1371/journal.pone.0150573WOS:000371724200103WOS000371724200103.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One1,164info:eu-repo/semantics/openAccess2023-12-19T06:25:46Zoai:repositorio.unesp.br:11449/161297Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-19T06:25:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Uncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae) |
title |
Uncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae) |
spellingShingle |
Uncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae) Utsunomia, Ricardo [UNESP] |
title_short |
Uncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae) |
title_full |
Uncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae) |
title_fullStr |
Uncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae) |
title_full_unstemmed |
Uncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae) |
title_sort |
Uncovering the Ancestry of B Chromosomes in Moenkhausia sanctaefilomenae (Teleostei, Characidae) |
author |
Utsunomia, Ricardo [UNESP] |
author_facet |
Utsunomia, Ricardo [UNESP] Zerbinato de Andrade Silva, Duilio Mazzoni [UNESP] Ruiz-Ruano, Francisco J. Araya-Jaime, Cristian [UNESP] Pansonato-Alves, Jose Carlos [UNESP] Scacchetti, Priscilla Cardim [UNESP] Hashimoto, Diogo Teruo [UNESP] Oliveira, Claudio [UNESP] Trifonov, Vladmir A. Porto-Foresti, Fabio [UNESP] Camacho, Juan Pedro M. Foresti, Fausto [UNESP] |
author_role |
author |
author2 |
Zerbinato de Andrade Silva, Duilio Mazzoni [UNESP] Ruiz-Ruano, Francisco J. Araya-Jaime, Cristian [UNESP] Pansonato-Alves, Jose Carlos [UNESP] Scacchetti, Priscilla Cardim [UNESP] Hashimoto, Diogo Teruo [UNESP] Oliveira, Claudio [UNESP] Trifonov, Vladmir A. Porto-Foresti, Fabio [UNESP] Camacho, Juan Pedro M. Foresti, Fausto [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Granada Inst Mol & Cellular Biol SB RAS |
dc.contributor.author.fl_str_mv |
Utsunomia, Ricardo [UNESP] Zerbinato de Andrade Silva, Duilio Mazzoni [UNESP] Ruiz-Ruano, Francisco J. Araya-Jaime, Cristian [UNESP] Pansonato-Alves, Jose Carlos [UNESP] Scacchetti, Priscilla Cardim [UNESP] Hashimoto, Diogo Teruo [UNESP] Oliveira, Claudio [UNESP] Trifonov, Vladmir A. Porto-Foresti, Fabio [UNESP] Camacho, Juan Pedro M. Foresti, Fausto [UNESP] |
description |
B chromosomes constitute a heterogeneous mixture of genomic parasites that are sometimes derived intraspecifically from the standard genome of the host species, but result from interspecific hybridization in other cases. The mode of origin determines the DNA content, with the B chromosomes showing high similarity with the A genome in the first case, but presenting higher similarity with a different species in the second. The characid fish Moenkhausia sanctaefilomenae harbours highly invasive B chromosomes, which are present in all populations analyzed to date in the Parana and Tiete rivers. To investigate the origin of these B chromosomes, we analyzed two natural populations: one carrying B chromosomes and the other lacking them, using a combination of molecular cytogenetic techniques, nucleotide sequence analysis and high-throughput sequencing (Illumina HiSeq2000). Our results showed that i) B chromosomes have not yet reached the Paranapanema River basin; ii) B chromosomes are mitotically unstable; iii) there are two types of B chromosomes, the most frequent of which is lightly C-banded (similar to euchromatin in A chromosomes) (B-1), while the other is darkly C-banded (heterochromatin-like) (B-2); iv) the two B types contain the same tandem repeat DNA sequences (18S ribosomal DNA, H3 histone genes, MS3 and MS7 satellite DNA), with a higher content of 18S rDNA in the heterochromatic variant; v) all of these repetitive DNAs are present together only in the paracentromeric region of autosome pair no. 6, suggesting that the B chromosomes are derived from this A chromosome; vi) the two B chromosome variants show MS3 sequences that are highly divergent from each other and from the 0B genome, although the B-2-derived sequences exhibit higher similarity with the 0B genome (this suggests an independent origin of the two B variants, with the less frequent, B-2 type presumably being younger); and vii) the dN/dS ratio for the H3.2 histone gene is almost 4-6 times higher for B chromosomes than for A chromosome sequences, suggesting that purifying selection is relaxed for the DNA sequences located on the B chromosomes, presumably because they are mostly inactive. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-02 2018-11-26T16:27:56Z 2018-11-26T16:27:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0150573 Plos One. San Francisco: Public Library Science, v. 11, n. 3, 20 p., 2016. 1932-6203 http://hdl.handle.net/11449/161297 10.1371/journal.pone.0150573 WOS:000371724200103 WOS000371724200103.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0150573 http://hdl.handle.net/11449/161297 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 11, n. 3, 20 p., 2016. 1932-6203 10.1371/journal.pone.0150573 WOS:000371724200103 WOS000371724200103.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Plos One 1,164 |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
20 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
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Public Library Science |
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Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1803047023652372480 |