Electrical signalling on Bt and non-Bt cotton plants under stress by Aphis gossypii
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0249699 http://hdl.handle.net/11449/206194 |
Resumo: | Plants have developed various mechanisms to respond specifically to each biotrophic attack. It has been shown that the electrical signals emitted by plants are associated with herbivory stress responses and can lead to the activation of multiple defences. Bt cotton is a genetically modified pest-resistant plant that produces an insecticide from Bacillus thuringiensis (Bt) to control Lepidopteran species. Surprisingly, there is no study–yet, that characterizes the signalling mechanisms in transgenic cotton plants attacked by non-target insects, such as aphids. In this study, we characterized the production of electrical signals on Bt and non-Bt cotton plants infested with Aphis gossypii and, in addition, we characterized the dispersal behaviour of aphids to correlate this behaviour to plant signalling responses. Electrical signalling of the plants was recorded with an extracellular measurement technique. Impressively, our results showed that both Bt and non-Bt cotton varieties, when attacked by A. gossypii, emitted potential variation-type electrical signals and clearly showed the presence of distinct responses regarding their perception and the behaviour of aphids, with evidence of delay, in terms of signal amount, and almost twice the amount of Cry1F protein was observed on Bt cotton plants at the highest density of insects/plant. We present in our article some hypotheses that are based on plant physiology and insect behaviour to explain the responses found on Bt cotton plants under aphid stress. |
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Electrical signalling on Bt and non-Bt cotton plants under stress by Aphis gossypiiPlants have developed various mechanisms to respond specifically to each biotrophic attack. It has been shown that the electrical signals emitted by plants are associated with herbivory stress responses and can lead to the activation of multiple defences. Bt cotton is a genetically modified pest-resistant plant that produces an insecticide from Bacillus thuringiensis (Bt) to control Lepidopteran species. Surprisingly, there is no study–yet, that characterizes the signalling mechanisms in transgenic cotton plants attacked by non-target insects, such as aphids. In this study, we characterized the production of electrical signals on Bt and non-Bt cotton plants infested with Aphis gossypii and, in addition, we characterized the dispersal behaviour of aphids to correlate this behaviour to plant signalling responses. Electrical signalling of the plants was recorded with an extracellular measurement technique. Impressively, our results showed that both Bt and non-Bt cotton varieties, when attacked by A. gossypii, emitted potential variation-type electrical signals and clearly showed the presence of distinct responses regarding their perception and the behaviour of aphids, with evidence of delay, in terms of signal amount, and almost twice the amount of Cry1F protein was observed on Bt cotton plants at the highest density of insects/plant. We present in our article some hypotheses that are based on plant physiology and insect behaviour to explain the responses found on Bt cotton plants under aphid stress.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Entomology and Acarology Luiz de Queiroz College of Agriculture (ESALQ) University of São Paulo (USP)Department of Biological Sciences Luiz de Queiroz College of Agriculture (ESALQ) University of São Paulo (USP)Department of Biostatistics São Paulo State University (UNESP)Biological Control Unit Embrapa AlgodãoDepartment of Biostatistics São Paulo State University (UNESP)FAPESP: 2015/20380–8FAPESP: 2017/05953–7FAPESP: 2018/ 20435–5Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Pachú, Jéssica K.S.Macedo, Francynes C.O.Malaquias, José B. [UNESP]Ramalho, Francisco S.Oliveira, Ricardo F.Franco, Flávia PereiraGodoy, Wesley A.C.2021-06-25T10:28:04Z2021-06-25T10:28:04Z2021-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0249699PLoS ONE, v. 16, n. 4 April, 2021.1932-6203http://hdl.handle.net/11449/20619410.1371/journal.pone.02496992-s2.0-85104162779Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2021-10-22T22:17:21Zoai:repositorio.unesp.br:11449/206194Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:52:32.665087Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Electrical signalling on Bt and non-Bt cotton plants under stress by Aphis gossypii |
title |
Electrical signalling on Bt and non-Bt cotton plants under stress by Aphis gossypii |
spellingShingle |
Electrical signalling on Bt and non-Bt cotton plants under stress by Aphis gossypii Pachú, Jéssica K.S. |
title_short |
Electrical signalling on Bt and non-Bt cotton plants under stress by Aphis gossypii |
title_full |
Electrical signalling on Bt and non-Bt cotton plants under stress by Aphis gossypii |
title_fullStr |
Electrical signalling on Bt and non-Bt cotton plants under stress by Aphis gossypii |
title_full_unstemmed |
Electrical signalling on Bt and non-Bt cotton plants under stress by Aphis gossypii |
title_sort |
Electrical signalling on Bt and non-Bt cotton plants under stress by Aphis gossypii |
author |
Pachú, Jéssica K.S. |
author_facet |
Pachú, Jéssica K.S. Macedo, Francynes C.O. Malaquias, José B. [UNESP] Ramalho, Francisco S. Oliveira, Ricardo F. Franco, Flávia Pereira Godoy, Wesley A.C. |
author_role |
author |
author2 |
Macedo, Francynes C.O. Malaquias, José B. [UNESP] Ramalho, Francisco S. Oliveira, Ricardo F. Franco, Flávia Pereira Godoy, Wesley A.C. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Pachú, Jéssica K.S. Macedo, Francynes C.O. Malaquias, José B. [UNESP] Ramalho, Francisco S. Oliveira, Ricardo F. Franco, Flávia Pereira Godoy, Wesley A.C. |
description |
Plants have developed various mechanisms to respond specifically to each biotrophic attack. It has been shown that the electrical signals emitted by plants are associated with herbivory stress responses and can lead to the activation of multiple defences. Bt cotton is a genetically modified pest-resistant plant that produces an insecticide from Bacillus thuringiensis (Bt) to control Lepidopteran species. Surprisingly, there is no study–yet, that characterizes the signalling mechanisms in transgenic cotton plants attacked by non-target insects, such as aphids. In this study, we characterized the production of electrical signals on Bt and non-Bt cotton plants infested with Aphis gossypii and, in addition, we characterized the dispersal behaviour of aphids to correlate this behaviour to plant signalling responses. Electrical signalling of the plants was recorded with an extracellular measurement technique. Impressively, our results showed that both Bt and non-Bt cotton varieties, when attacked by A. gossypii, emitted potential variation-type electrical signals and clearly showed the presence of distinct responses regarding their perception and the behaviour of aphids, with evidence of delay, in terms of signal amount, and almost twice the amount of Cry1F protein was observed on Bt cotton plants at the highest density of insects/plant. We present in our article some hypotheses that are based on plant physiology and insect behaviour to explain the responses found on Bt cotton plants under aphid stress. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:28:04Z 2021-06-25T10:28:04Z 2021-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0249699 PLoS ONE, v. 16, n. 4 April, 2021. 1932-6203 http://hdl.handle.net/11449/206194 10.1371/journal.pone.0249699 2-s2.0-85104162779 |
url |
http://dx.doi.org/10.1371/journal.pone.0249699 http://hdl.handle.net/11449/206194 |
identifier_str_mv |
PLoS ONE, v. 16, n. 4 April, 2021. 1932-6203 10.1371/journal.pone.0249699 2-s2.0-85104162779 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129560851513344 |