Transcriptoma e peptidoma de tentáculos de Bunodosoma caissarum CORRÊA, 1964

• Autor(a) principal: Mazzi, Maria Eduarda Esquinca
• Data de Publicação: 2022
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/216048
• topic: Anêmonas; Transcriptoma; Peptidoma; Espectrometria de massas

The marine environment is extremely diverse and has about 50% of the planet's species, which developed adaptive mechanisms that ensure their survival in the seas and oceans. These mechanisms occur at a morphological, physiological, behavioral and/or biochemical level, where one of the strategies used by animals is the production of poison that helps in defense or obtaining food. This secretion is loaded with biologically active molecules, such as proteins, peptides, salts and polyamines, which may have therapeutic potential. Among these molecules are peptides, amino acid sequences of up to 10 kDa that have a variety of functions and can act as regulatory molecules in cardiovascular, neurodegenerative, inflammatory and immunological diseases. Among the marine groups that produce venom are the cnidarians, releasing it through structures known as cnidocytes. In this context, this project used next generation sequencing and mass spectrometry to characterize transcripts and peptides from a marine anemone species widely distributed along the Brazilian coast: Bunodosoma caissarum (B. caissarum). The transcriptome analysis resulted in the annotation of 23,444 genes, of which 48 toxins produced by species belonging to the cnidarians were identified. Additionally, 15 of these toxins were confirmed by mucus proteomics experiments, three of which were previously described in B. caissarum. The transcriptome integrated to mass spectrometry data resulted in the identification of 2102 peptide sequences. Therefore, this study enriched the database of species, an initial step for future investigation of molecules with pharmacological potential.