Use of weathered diesel oil as a low-cost raw material to biosurfactant production
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/228237 |
Resumo: | This work aimed to investigate the capability of biosurfactant production by bacteria cultures of Staphylococcus hominis, Kocuria palustris and Pseudomonas aeruginosa LBI, isolated from hydrocarbon contaminated areas, using weathered diesel oil from a long-standing spillage as raw material. It was evaluated the influence of the culture media (Robert or Bushnell-Haas) and of the carbon source (spilled diesel oil or commercial diesel oil) in the biosurfactant production. Erlenmeyer flasks (250 mL) containing the cell broth were agitated (240 rpm) for 144 h. The monitoring of the biosurfactant production was carried out measuring the surface tension of the cell free culture, according to the De Nöuy ring method using the Krüss K6 tensiometer. Considering the possibility of intracellular storage of biosurfactant in the cell wall of the cultures S. hominis and K. palustris, tests were also done applying ultrasound as a way to rupture the cells. For the studied conditions, the cultures did not indicate biosurfactants production. Results obtained with a hydrocarbon biodegradability test employing the redox indicator 2,6 - dichlorophenol indophenol showed that only the commercial diesel is biodegraded by the cultures. |
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Use of weathered diesel oil as a low-cost raw material to biosurfactant productionBiosurfactantRaw materialWeathered diesel oilThis work aimed to investigate the capability of biosurfactant production by bacteria cultures of Staphylococcus hominis, Kocuria palustris and Pseudomonas aeruginosa LBI, isolated from hydrocarbon contaminated areas, using weathered diesel oil from a long-standing spillage as raw material. It was evaluated the influence of the culture media (Robert or Bushnell-Haas) and of the carbon source (spilled diesel oil or commercial diesel oil) in the biosurfactant production. Erlenmeyer flasks (250 mL) containing the cell broth were agitated (240 rpm) for 144 h. The monitoring of the biosurfactant production was carried out measuring the surface tension of the cell free culture, according to the De Nöuy ring method using the Krüss K6 tensiometer. Considering the possibility of intracellular storage of biosurfactant in the cell wall of the cultures S. hominis and K. palustris, tests were also done applying ultrasound as a way to rupture the cells. For the studied conditions, the cultures did not indicate biosurfactants production. Results obtained with a hydrocarbon biodegradability test employing the redox indicator 2,6 - dichlorophenol indophenol showed that only the commercial diesel is biodegraded by the cultures.Instituto de Geociências e Ciências Exatas (IGCE), Av. 24-A, 1515 - CP 178Departamento de Bioquímica e Microbiologia Instituto de Biociências (IB), Av. 24-A, 1515 - CP 178Programa de Recursos Humanos (PRH 05) Agência Nacional do Petróleo Gás Natural e Biocombustíveis (ANP) Universidade Estadual Paulista (Unesp), Av. 24-A, 1515 - CP 178Programa de Recursos Humanos (PRH 05) Agência Nacional do Petróleo Gás Natural e Biocombustíveis (ANP) Universidade Estadual Paulista (Unesp), Av. 24-A, 1515 - CP 178Instituto de Geociências e Ciências Exatas (IGCE)Instituto de Biociências (IB)Universidade Estadual Paulista (UNESP)Mariano, Adriano Pinto [UNESP]Bonotto, Daniel MarcosDe Franceschi De Angelis, DejaniraPirôllo, Maria Paula Santos [UNESP]Contiero, Jonas2022-04-29T07:53:46Z2022-04-29T07:53:46Z2006-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectXXII Interamerican Congress of Chemical Engineering, CIIQ 2006 and V Argentinian Congress of Chemical Engineering, CAIQ 2006 - Innovation and Management for Sustainable Development.http://hdl.handle.net/11449/2282372-s2.0-84992390344Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengXXII Interamerican Congress of Chemical Engineering, CIIQ 2006 and V Argentinian Congress of Chemical Engineering, CAIQ 2006 - Innovation and Management for Sustainable Developmentinfo:eu-repo/semantics/openAccess2022-04-29T07:53:46Zoai:repositorio.unesp.br:11449/228237Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T07:53:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Use of weathered diesel oil as a low-cost raw material to biosurfactant production |
title |
Use of weathered diesel oil as a low-cost raw material to biosurfactant production |
spellingShingle |
Use of weathered diesel oil as a low-cost raw material to biosurfactant production Mariano, Adriano Pinto [UNESP] Biosurfactant Raw material Weathered diesel oil |
title_short |
Use of weathered diesel oil as a low-cost raw material to biosurfactant production |
title_full |
Use of weathered diesel oil as a low-cost raw material to biosurfactant production |
title_fullStr |
Use of weathered diesel oil as a low-cost raw material to biosurfactant production |
title_full_unstemmed |
Use of weathered diesel oil as a low-cost raw material to biosurfactant production |
title_sort |
Use of weathered diesel oil as a low-cost raw material to biosurfactant production |
author |
Mariano, Adriano Pinto [UNESP] |
author_facet |
Mariano, Adriano Pinto [UNESP] Bonotto, Daniel Marcos De Franceschi De Angelis, Dejanira Pirôllo, Maria Paula Santos [UNESP] Contiero, Jonas |
author_role |
author |
author2 |
Bonotto, Daniel Marcos De Franceschi De Angelis, Dejanira Pirôllo, Maria Paula Santos [UNESP] Contiero, Jonas |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Instituto de Geociências e Ciências Exatas (IGCE) Instituto de Biociências (IB) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Mariano, Adriano Pinto [UNESP] Bonotto, Daniel Marcos De Franceschi De Angelis, Dejanira Pirôllo, Maria Paula Santos [UNESP] Contiero, Jonas |
dc.subject.por.fl_str_mv |
Biosurfactant Raw material Weathered diesel oil |
topic |
Biosurfactant Raw material Weathered diesel oil |
description |
This work aimed to investigate the capability of biosurfactant production by bacteria cultures of Staphylococcus hominis, Kocuria palustris and Pseudomonas aeruginosa LBI, isolated from hydrocarbon contaminated areas, using weathered diesel oil from a long-standing spillage as raw material. It was evaluated the influence of the culture media (Robert or Bushnell-Haas) and of the carbon source (spilled diesel oil or commercial diesel oil) in the biosurfactant production. Erlenmeyer flasks (250 mL) containing the cell broth were agitated (240 rpm) for 144 h. The monitoring of the biosurfactant production was carried out measuring the surface tension of the cell free culture, according to the De Nöuy ring method using the Krüss K6 tensiometer. Considering the possibility of intracellular storage of biosurfactant in the cell wall of the cultures S. hominis and K. palustris, tests were also done applying ultrasound as a way to rupture the cells. For the studied conditions, the cultures did not indicate biosurfactants production. Results obtained with a hydrocarbon biodegradability test employing the redox indicator 2,6 - dichlorophenol indophenol showed that only the commercial diesel is biodegraded by the cultures. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 2022-04-29T07:53:46Z 2022-04-29T07:53:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
XXII Interamerican Congress of Chemical Engineering, CIIQ 2006 and V Argentinian Congress of Chemical Engineering, CAIQ 2006 - Innovation and Management for Sustainable Development. http://hdl.handle.net/11449/228237 2-s2.0-84992390344 |
identifier_str_mv |
XXII Interamerican Congress of Chemical Engineering, CIIQ 2006 and V Argentinian Congress of Chemical Engineering, CAIQ 2006 - Innovation and Management for Sustainable Development. 2-s2.0-84992390344 |
url |
http://hdl.handle.net/11449/228237 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
XXII Interamerican Congress of Chemical Engineering, CIIQ 2006 and V Argentinian Congress of Chemical Engineering, CAIQ 2006 - Innovation and Management for Sustainable Development |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046835483312128 |