Stimulation of morphofunctional repair of the facial nerve with photobiomodulation, using the end-to-side technique or a new heterologous fibrin sealant
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jphotobiol.2017.08.023 http://hdl.handle.net/11449/175079 |
Resumo: | This research evaluated the influence of Photobiomodulation Therapy (PBMT) on lesions of the facial nerve repaired with the end-to-side technique or coaptation with a new heterologous fibrin sealant. Thirty-two Wistar rats were separated into 5 groups: Control group (CG), where the buccal branch of the facial nerve was collected; Experimental Suture Group (ESG) and Experimental Fibrin Group (EFG), in which the buccal branch was end-to-side sutured to the zygomatic branch on the right side of the face or coaptated with fibrin sealant on the left side; Experimental Suture Laser Group (ESLG) and Experimental Fibrin Laser Group (EFLG), in which the same procedures were performed as the ESG and EFG, associated with PBMT (wavelength of 830 nm, energy density 6.2 J/cm2, power output 30 mW, beam area of 0.116 cm2, power density 0.26 W/cm2, total energy per session 2.16 J, cumulative dose of 34.56 J). The laser was applied for 24 s/site at 3 points on the skin's surface, for a total application time of 72 s, performed immediately after surgery and 3 times a week for 5 weeks. A statistically significant difference was observed in the fiber nerve area between the EFG and EFLG (57.49 ± 3.13 and 62.52 ± 3.56 μm2, respectively). For the area of the axon, fiber diameter, axon diameter, myelin sheath area and myelin sheath thickness no statistically significant differences were found (p < 0.05). The functional recovery of whisker movement occurred faster in the ESLG and EFLG, which were associated with PBMT, with results closer to the CG. Therefore, PBMT accelerated morphological and functional nerve repair in both techniques. |
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Stimulation of morphofunctional repair of the facial nerve with photobiomodulation, using the end-to-side technique or a new heterologous fibrin sealantFacial nerveFibrin tissue adhesiveLow-level laser therapyNerve regenerationThis research evaluated the influence of Photobiomodulation Therapy (PBMT) on lesions of the facial nerve repaired with the end-to-side technique or coaptation with a new heterologous fibrin sealant. Thirty-two Wistar rats were separated into 5 groups: Control group (CG), where the buccal branch of the facial nerve was collected; Experimental Suture Group (ESG) and Experimental Fibrin Group (EFG), in which the buccal branch was end-to-side sutured to the zygomatic branch on the right side of the face or coaptated with fibrin sealant on the left side; Experimental Suture Laser Group (ESLG) and Experimental Fibrin Laser Group (EFLG), in which the same procedures were performed as the ESG and EFG, associated with PBMT (wavelength of 830 nm, energy density 6.2 J/cm2, power output 30 mW, beam area of 0.116 cm2, power density 0.26 W/cm2, total energy per session 2.16 J, cumulative dose of 34.56 J). The laser was applied for 24 s/site at 3 points on the skin's surface, for a total application time of 72 s, performed immediately after surgery and 3 times a week for 5 weeks. A statistically significant difference was observed in the fiber nerve area between the EFG and EFLG (57.49 ± 3.13 and 62.52 ± 3.56 μm2, respectively). For the area of the axon, fiber diameter, axon diameter, myelin sheath area and myelin sheath thickness no statistically significant differences were found (p < 0.05). The functional recovery of whisker movement occurred faster in the ESLG and EFLG, which were associated with PBMT, with results closer to the CG. Therefore, PBMT accelerated morphological and functional nerve repair in both techniques.Department of Biological Sciences (Anatomy) Bauru School of Dentistry University of São Paulo (USP)Health Sciences Center University of the Sacred Heart (USC)Human Morphophysiology (Anatomy) University of Marilia (UNIMAR)Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (Univ. Estadual Paulista UNESP)Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (Univ. Estadual Paulista UNESP)Universidade de São Paulo (USP)University of the Sacred Heart (USC)University of Marilia (UNIMAR)Universidade Estadual Paulista (Unesp)Rosso, Marcelie Priscila de OliveiraRosa Júnior, Geraldo MarcoBuchaim, Daniela VieiraGerman, Iris Jasmin SantosPomini, Karina Torresde Souza, Rafael GomesPereira, MizaelFavaretto Júnior, Idvaldo AparecidoBueno, Cleuber Rodrigo de SouzaGonçalves, Jéssica Barbosa de OliveiraFerreira Júnior, Rui Seabra [UNESP]Barraviera, Benedito [UNESP]Andreo, Jesus CarlosBuchaim, Rogério Leone2018-12-11T17:14:16Z2018-12-11T17:14:16Z2017-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20-28application/pdfhttp://dx.doi.org/10.1016/j.jphotobiol.2017.08.023Journal of Photochemistry and Photobiology B: Biology, v. 175, p. 20-28.1873-26821011-1344http://hdl.handle.net/11449/17507910.1016/j.jphotobiol.2017.08.0232-s2.0-850280541362-s2.0-85028054136.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Photochemistry and Photobiology B: Biology0,698info:eu-repo/semantics/openAccess2024-04-11T15:28:17Zoai:repositorio.unesp.br:11449/175079Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:59:07.642431Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Stimulation of morphofunctional repair of the facial nerve with photobiomodulation, using the end-to-side technique or a new heterologous fibrin sealant |
title |
Stimulation of morphofunctional repair of the facial nerve with photobiomodulation, using the end-to-side technique or a new heterologous fibrin sealant |
spellingShingle |
Stimulation of morphofunctional repair of the facial nerve with photobiomodulation, using the end-to-side technique or a new heterologous fibrin sealant Rosso, Marcelie Priscila de Oliveira Facial nerve Fibrin tissue adhesive Low-level laser therapy Nerve regeneration |
title_short |
Stimulation of morphofunctional repair of the facial nerve with photobiomodulation, using the end-to-side technique or a new heterologous fibrin sealant |
title_full |
Stimulation of morphofunctional repair of the facial nerve with photobiomodulation, using the end-to-side technique or a new heterologous fibrin sealant |
title_fullStr |
Stimulation of morphofunctional repair of the facial nerve with photobiomodulation, using the end-to-side technique or a new heterologous fibrin sealant |
title_full_unstemmed |
Stimulation of morphofunctional repair of the facial nerve with photobiomodulation, using the end-to-side technique or a new heterologous fibrin sealant |
title_sort |
Stimulation of morphofunctional repair of the facial nerve with photobiomodulation, using the end-to-side technique or a new heterologous fibrin sealant |
author |
Rosso, Marcelie Priscila de Oliveira |
author_facet |
Rosso, Marcelie Priscila de Oliveira Rosa Júnior, Geraldo Marco Buchaim, Daniela Vieira German, Iris Jasmin Santos Pomini, Karina Torres de Souza, Rafael Gomes Pereira, Mizael Favaretto Júnior, Idvaldo Aparecido Bueno, Cleuber Rodrigo de Souza Gonçalves, Jéssica Barbosa de Oliveira Ferreira Júnior, Rui Seabra [UNESP] Barraviera, Benedito [UNESP] Andreo, Jesus Carlos Buchaim, Rogério Leone |
author_role |
author |
author2 |
Rosa Júnior, Geraldo Marco Buchaim, Daniela Vieira German, Iris Jasmin Santos Pomini, Karina Torres de Souza, Rafael Gomes Pereira, Mizael Favaretto Júnior, Idvaldo Aparecido Bueno, Cleuber Rodrigo de Souza Gonçalves, Jéssica Barbosa de Oliveira Ferreira Júnior, Rui Seabra [UNESP] Barraviera, Benedito [UNESP] Andreo, Jesus Carlos Buchaim, Rogério Leone |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) University of the Sacred Heart (USC) University of Marilia (UNIMAR) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rosso, Marcelie Priscila de Oliveira Rosa Júnior, Geraldo Marco Buchaim, Daniela Vieira German, Iris Jasmin Santos Pomini, Karina Torres de Souza, Rafael Gomes Pereira, Mizael Favaretto Júnior, Idvaldo Aparecido Bueno, Cleuber Rodrigo de Souza Gonçalves, Jéssica Barbosa de Oliveira Ferreira Júnior, Rui Seabra [UNESP] Barraviera, Benedito [UNESP] Andreo, Jesus Carlos Buchaim, Rogério Leone |
dc.subject.por.fl_str_mv |
Facial nerve Fibrin tissue adhesive Low-level laser therapy Nerve regeneration |
topic |
Facial nerve Fibrin tissue adhesive Low-level laser therapy Nerve regeneration |
description |
This research evaluated the influence of Photobiomodulation Therapy (PBMT) on lesions of the facial nerve repaired with the end-to-side technique or coaptation with a new heterologous fibrin sealant. Thirty-two Wistar rats were separated into 5 groups: Control group (CG), where the buccal branch of the facial nerve was collected; Experimental Suture Group (ESG) and Experimental Fibrin Group (EFG), in which the buccal branch was end-to-side sutured to the zygomatic branch on the right side of the face or coaptated with fibrin sealant on the left side; Experimental Suture Laser Group (ESLG) and Experimental Fibrin Laser Group (EFLG), in which the same procedures were performed as the ESG and EFG, associated with PBMT (wavelength of 830 nm, energy density 6.2 J/cm2, power output 30 mW, beam area of 0.116 cm2, power density 0.26 W/cm2, total energy per session 2.16 J, cumulative dose of 34.56 J). The laser was applied for 24 s/site at 3 points on the skin's surface, for a total application time of 72 s, performed immediately after surgery and 3 times a week for 5 weeks. A statistically significant difference was observed in the fiber nerve area between the EFG and EFLG (57.49 ± 3.13 and 62.52 ± 3.56 μm2, respectively). For the area of the axon, fiber diameter, axon diameter, myelin sheath area and myelin sheath thickness no statistically significant differences were found (p < 0.05). The functional recovery of whisker movement occurred faster in the ESLG and EFLG, which were associated with PBMT, with results closer to the CG. Therefore, PBMT accelerated morphological and functional nerve repair in both techniques. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-01 2018-12-11T17:14:16Z 2018-12-11T17:14:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jphotobiol.2017.08.023 Journal of Photochemistry and Photobiology B: Biology, v. 175, p. 20-28. 1873-2682 1011-1344 http://hdl.handle.net/11449/175079 10.1016/j.jphotobiol.2017.08.023 2-s2.0-85028054136 2-s2.0-85028054136.pdf |
url |
http://dx.doi.org/10.1016/j.jphotobiol.2017.08.023 http://hdl.handle.net/11449/175079 |
identifier_str_mv |
Journal of Photochemistry and Photobiology B: Biology, v. 175, p. 20-28. 1873-2682 1011-1344 10.1016/j.jphotobiol.2017.08.023 2-s2.0-85028054136 2-s2.0-85028054136.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Photochemistry and Photobiology B: Biology 0,698 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
20-28 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128881547280384 |