Hypotensive effect of Eugenia dysenterica leaf extract is primarily related to its vascular action: The possible underlying mechanisms
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jep.2019.112520 http://hdl.handle.net/11449/198323 |
Resumo: | Ethnopharmacological relevance: Eugenia dysenterica (ED) leaves are used in Brazil to treat cardiac diseases; however, there are no scientific data describing the effects of this species on cardiac activity. Aim of the study: To investigate the effect of ED aqueous leaf extract (EDLE) on hear rate (HR) and mean arterial pressure (MAP) of anaesthetised rats and its underlying mechanism of action. Material and methods: EDLE was analysed, and its proanthocyanidin composition was determined. After performing dose-effect curves for EDLE on HR and MAP, EDLE-induced hypotension was evaluated before and after atropine (AT), L-N(ω)-nitro-L-arginine methyl ester (L-NAME), hexamethonium (HXT), indomethacin (IND), carbenoxolone (CBX), or nifedipine (NFD) administration. The effect of proanthocyanidin-depleted extract (EDLE/P-) was also determined and compared to that of the EDLE with proanthocyanidins. Results: EDLE decreased the MAP in a dose-dependent manner; HR was decreased only with the highest and most toxic dose. Only CBX and NFD decreased EDLE-induced hypotension. Five polymeric series of proanthocyanidins were identified, which were mainly constituted by procyanidin and prodelphinidin units with B-type linkage and up to 12 flavan-3-ol units. EDLE/P- induced hypotension did not differ from that induced by EDLE. Conclusions: The cardiovascular effects of EDLE were primarily related to its vascular action. EDLE-induced hypotensive effect appeared to involve L-type calcium channel blockage as well as myoendothelial gap junction signalling. The higher molecular weight proanthocyanidins from EDLE are unlikely to contribute to its cardiovascular effect. |
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Hypotensive effect of Eugenia dysenterica leaf extract is primarily related to its vascular action: The possible underlying mechanismsEugenia dysentericaHeartHypotensionLeavesMALDI-TOFProanthocyanidinsEthnopharmacological relevance: Eugenia dysenterica (ED) leaves are used in Brazil to treat cardiac diseases; however, there are no scientific data describing the effects of this species on cardiac activity. Aim of the study: To investigate the effect of ED aqueous leaf extract (EDLE) on hear rate (HR) and mean arterial pressure (MAP) of anaesthetised rats and its underlying mechanism of action. Material and methods: EDLE was analysed, and its proanthocyanidin composition was determined. After performing dose-effect curves for EDLE on HR and MAP, EDLE-induced hypotension was evaluated before and after atropine (AT), L-N(ω)-nitro-L-arginine methyl ester (L-NAME), hexamethonium (HXT), indomethacin (IND), carbenoxolone (CBX), or nifedipine (NFD) administration. The effect of proanthocyanidin-depleted extract (EDLE/P-) was also determined and compared to that of the EDLE with proanthocyanidins. Results: EDLE decreased the MAP in a dose-dependent manner; HR was decreased only with the highest and most toxic dose. Only CBX and NFD decreased EDLE-induced hypotension. Five polymeric series of proanthocyanidins were identified, which were mainly constituted by procyanidin and prodelphinidin units with B-type linkage and up to 12 flavan-3-ol units. EDLE/P- induced hypotension did not differ from that induced by EDLE. Conclusions: The cardiovascular effects of EDLE were primarily related to its vascular action. EDLE-induced hypotensive effect appeared to involve L-type calcium channel blockage as well as myoendothelial gap junction signalling. The higher molecular weight proanthocyanidins from EDLE are unlikely to contribute to its cardiovascular effect.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University/UNESP Department of Physiology Institute of BiosciencesFederal University of Mato Grosso do Sul/UFMS Laboratório de Produtos Naturais e Espectrometria de Massas (LAPNEM)Federal University of Uberlândia/UFU Department of Pharmacology Institute of Biomedical Sciences Campus UmuaramaSão Paulo State University/UNESP Department of Physiology Institute of BiosciencesFAPESP: 2013/18382-7Universidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso do Sul (UFMS)Universidade Federal de Uberlândia (UFU)Fidelis-de-Oliveira, Patrícia [UNESP]Aparecida-Castro, Samanta [UNESP]Silva, Denise BrentanMorais, Ingrid Beatriz de MeloMiranda, Victor Hugo Martins de [UNESP]de Gobbi, Juliana Irani [UNESP]Canabrava, Hudson Armando NunesBispo-da-Silva, Luiz Borges2020-12-12T01:09:38Z2020-12-12T01:09:38Z2020-04-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jep.2019.112520Journal of Ethnopharmacology, v. 251.1872-75730378-8741http://hdl.handle.net/11449/19832310.1016/j.jep.2019.1125202-s2.0-85077092443Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Ethnopharmacologyinfo:eu-repo/semantics/openAccess2021-10-23T09:49:05Zoai:repositorio.unesp.br:11449/198323Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:53:58.772977Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hypotensive effect of Eugenia dysenterica leaf extract is primarily related to its vascular action: The possible underlying mechanisms |
title |
Hypotensive effect of Eugenia dysenterica leaf extract is primarily related to its vascular action: The possible underlying mechanisms |
spellingShingle |
Hypotensive effect of Eugenia dysenterica leaf extract is primarily related to its vascular action: The possible underlying mechanisms Fidelis-de-Oliveira, Patrícia [UNESP] Eugenia dysenterica Heart Hypotension Leaves MALDI-TOF Proanthocyanidins |
title_short |
Hypotensive effect of Eugenia dysenterica leaf extract is primarily related to its vascular action: The possible underlying mechanisms |
title_full |
Hypotensive effect of Eugenia dysenterica leaf extract is primarily related to its vascular action: The possible underlying mechanisms |
title_fullStr |
Hypotensive effect of Eugenia dysenterica leaf extract is primarily related to its vascular action: The possible underlying mechanisms |
title_full_unstemmed |
Hypotensive effect of Eugenia dysenterica leaf extract is primarily related to its vascular action: The possible underlying mechanisms |
title_sort |
Hypotensive effect of Eugenia dysenterica leaf extract is primarily related to its vascular action: The possible underlying mechanisms |
author |
Fidelis-de-Oliveira, Patrícia [UNESP] |
author_facet |
Fidelis-de-Oliveira, Patrícia [UNESP] Aparecida-Castro, Samanta [UNESP] Silva, Denise Brentan Morais, Ingrid Beatriz de Melo Miranda, Victor Hugo Martins de [UNESP] de Gobbi, Juliana Irani [UNESP] Canabrava, Hudson Armando Nunes Bispo-da-Silva, Luiz Borges |
author_role |
author |
author2 |
Aparecida-Castro, Samanta [UNESP] Silva, Denise Brentan Morais, Ingrid Beatriz de Melo Miranda, Victor Hugo Martins de [UNESP] de Gobbi, Juliana Irani [UNESP] Canabrava, Hudson Armando Nunes Bispo-da-Silva, Luiz Borges |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Mato Grosso do Sul (UFMS) Universidade Federal de Uberlândia (UFU) |
dc.contributor.author.fl_str_mv |
Fidelis-de-Oliveira, Patrícia [UNESP] Aparecida-Castro, Samanta [UNESP] Silva, Denise Brentan Morais, Ingrid Beatriz de Melo Miranda, Victor Hugo Martins de [UNESP] de Gobbi, Juliana Irani [UNESP] Canabrava, Hudson Armando Nunes Bispo-da-Silva, Luiz Borges |
dc.subject.por.fl_str_mv |
Eugenia dysenterica Heart Hypotension Leaves MALDI-TOF Proanthocyanidins |
topic |
Eugenia dysenterica Heart Hypotension Leaves MALDI-TOF Proanthocyanidins |
description |
Ethnopharmacological relevance: Eugenia dysenterica (ED) leaves are used in Brazil to treat cardiac diseases; however, there are no scientific data describing the effects of this species on cardiac activity. Aim of the study: To investigate the effect of ED aqueous leaf extract (EDLE) on hear rate (HR) and mean arterial pressure (MAP) of anaesthetised rats and its underlying mechanism of action. Material and methods: EDLE was analysed, and its proanthocyanidin composition was determined. After performing dose-effect curves for EDLE on HR and MAP, EDLE-induced hypotension was evaluated before and after atropine (AT), L-N(ω)-nitro-L-arginine methyl ester (L-NAME), hexamethonium (HXT), indomethacin (IND), carbenoxolone (CBX), or nifedipine (NFD) administration. The effect of proanthocyanidin-depleted extract (EDLE/P-) was also determined and compared to that of the EDLE with proanthocyanidins. Results: EDLE decreased the MAP in a dose-dependent manner; HR was decreased only with the highest and most toxic dose. Only CBX and NFD decreased EDLE-induced hypotension. Five polymeric series of proanthocyanidins were identified, which were mainly constituted by procyanidin and prodelphinidin units with B-type linkage and up to 12 flavan-3-ol units. EDLE/P- induced hypotension did not differ from that induced by EDLE. Conclusions: The cardiovascular effects of EDLE were primarily related to its vascular action. EDLE-induced hypotensive effect appeared to involve L-type calcium channel blockage as well as myoendothelial gap junction signalling. The higher molecular weight proanthocyanidins from EDLE are unlikely to contribute to its cardiovascular effect. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:09:38Z 2020-12-12T01:09:38Z 2020-04-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jep.2019.112520 Journal of Ethnopharmacology, v. 251. 1872-7573 0378-8741 http://hdl.handle.net/11449/198323 10.1016/j.jep.2019.112520 2-s2.0-85077092443 |
url |
http://dx.doi.org/10.1016/j.jep.2019.112520 http://hdl.handle.net/11449/198323 |
identifier_str_mv |
Journal of Ethnopharmacology, v. 251. 1872-7573 0378-8741 10.1016/j.jep.2019.112520 2-s2.0-85077092443 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Ethnopharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128227234807808 |