Serjania marginata Casar. Hydroalcoholic Extract Reduced Cytokine and Inflammatory Parameters in Experimental Models of Inflammation and Infection in Mice

• Autor(a) principal: Leitao, Maicon Matos
• Data de Publicação: 2022
• Outros Autores: Radai, Joyce Alencar Santos, Macorini, Luis Fernando Benitez, Fraga, Thiago Leite, Heredia-Vieira, Silvia Cristina, Cardoso, Claudia Andrea Lima, Arena, Arielle Cristina [UNESP], Kassuya, Candida Aparecida Leite
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.5530/pres.14.2.18; http://hdl.handle.net/11449/237772
• topic: Mycobacterium tuberculosis; Bacillus Calmette-Guerin (BCG); Cipo-timbo; Inflammation; Tuberculosis

Objectives: This study investigated the antimycobacterial, anti-inflammatory and antihyperalgesic effects of hydroalcoholic extract from leaves of S. marginata (EESM) in in vitro and in vivo models. Methods and Results: EESM (0.98-1000 mu g/ml) was evaluated in in vitro against Mycobacterium tuberculosis, M. bovis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus epidermidis. The EESM oral administration (p.o.) (30, 100 and 300 mg/kg) and dexamethasone subcutaneous injection (s.c.) (1 mg/kg) were tested against the carrageenan-induced inflammatory paw edema and pleurisy in Swiss mice. The EESM (30 and 100 mg/kg, p.o.) and dexamethasone (1 mg/kg, s.c.) were tested against the CFA-induced paw inflammation and M. bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice. The minimum inhibitory concentration (MIC) of EESM in the presence of M. tuberculosis was 62.4 mu g/ml. The values of MIC of EESM in the presence S. epidermidis, K. pneumoniae were 1000 mu g/mL while EESM did not interfere against P. aeruginosa growth. EESM significantly inhibited paw edema/mechanical hyperalgesia in carrageenan induced paw inflammation and leukocytes migration/proteins exudation in carrageenan-induced pleurisy model. In the BCGinduced pleurisy model, the daily treatment for 7 days, with EESM inhibited the levels of IL-1 beta in blood and in pleural exudate. The EESM did not alter the mycobacterial growth in the cell culture from pleural lavage, spleen and liver samples collected from BCG-treated animals. The EESM significantly inhibited the persistent edema and mechanical hyperalgesia induced by CFA. Conclusion: This study confirms the EESM anti-inflammatory property and showed that EESM has high potency in inducing inhibition of mycobaterial growth and low potency or no effects in relation to other microorganisms.