The influence of violet LED application time on the esthetic efficacy and cytotoxicity of a 35% H2O2 bleaching gel

• Autor(a) principal: Ribeiro, Rafael Antonio de Oliveira [UNESP]
• Data de Publicação: 2022
• Outros Autores: Peruchi, Victória [UNESP], Fernandes, Lídia de Oliveira [UNESP], Anselmi, Caroline [UNESP], Soares, Igor Paulino Mendes [UNESP], Hebling, Josimeri [UNESP], Costa, Carlos Alberto de Souza [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.pdpdt.2022.103069; http://hdl.handle.net/11449/247677
• topic: Cytotoxicity; Odontoblasts; Tooth bleaching; Violet LED

Objective: To assess the potential influence of violet LED (V-LED) application time on the esthetic efficacy and cytotoxicity of a 35% H2O2 bleaching gel. Methodology: Stained and standardized enamel/dentin discs were subjected to one in-office tooth bleaching session (45 min), and the gel was either irradiated or not with V-LED. Thus, the following groups were established (n = 8): G1: No treatment (negative control, NC); G2: 35% H2O2 (positive control, PC); G3: 35%H2O2 + V-LED/15 min; G4: 35%H2O2 + V-LED/30 min; G5: 35%H2O2 + V-LED/45 min. First, esthetic efficacy was assessed (ΔE00 and ΔWI). Discs assembled in artificial pulp chambers were subjected to the same bleaching treatments. Then, the extracts (culture medium + diffused bleaching gel components) were collected and applied to MDPC-23 pulp cells, which were analyzed for viability (Live/Dead, MTT) and oxidative stress (OxS). The amount of H2O2 in the extracts was also determined (leuco crystal-violet/peroxidase). The data were subjected to ANOVA/Tukey at a 5% significance level. Results: Although esthetic efficacy did not differ among the irradiated groups (G3, G4, and G5) (p > 0.05), their results were higher than in G2 (PC; p < 0.05). In the irradiated groups, the cell viability and OxS as well as the amount of H2O2 in the extracts were statistically similar to G2 (PC), regardless of irradiation time (p > 0.05). Conclusion: Although V-LED improves the esthetic outcome of in-office tooth bleaching, increasing irradiation time does not effect the color changes and cytotoxicity of this professional therapy.