Cytogenetic and molecular characteristics of potamotrygon motoro and potamotrygon sp. (chondrichthyes, myliobatiformes, potamotrygonidae) from the amazon basin: Implications for the taxonomy of the genus
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-4685-gmb-2020-0083 http://hdl.handle.net/11449/207619 |
Resumo: | The chromosomes of two freshwater stingrays, Potamotrygon motoro and Potamotrygon sp., from the Amazon River basin in Brazil were investigated using integrated molecular (cytochrome c oxidase subunit 1) and cytogenetic analyses. Potamotrygon motoro presented intraspecific variation in the diploid number, with 2n=66 in the females and 2n=65 in the males, while Potamotrygon sp. had a karyotype with 66 chromosomes, in both sexes. The C-banding revealed the presence of heterochromatic blocks accumulated in the centromeric region of all the chromosomes in both species. The FISH assays with 18S DNA probes highlighted the terminal region of three or four chromosome pairs in P. motoro and seven chromosomes in Potamotrygon sp. The rDNA 5S sequences were found in only one chromosomal pair in both species. The interspecific genetic distance based on the COI sequences, between P. motoro and Potamotrygon sp. from Amazon River was 10.8%, while that between the Amazonian P. motoro and Potamotrygon amandae from the Paraná River was 2.2%, and the genetic distance between Potamotrygon sp. and P. amandae was 11.8%. In addition to the new insights on the cytogenetics of the study species, the results of the present study confirmed the existence of heteromorphic sex-linked chromosomes in P. motoro. |
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Cytogenetic and molecular characteristics of potamotrygon motoro and potamotrygon sp. (chondrichthyes, myliobatiformes, potamotrygonidae) from the amazon basin: Implications for the taxonomy of the genusBiodiversitDNA barcodeFish chromosomesFreshwater stingraysPotamotrygonThe chromosomes of two freshwater stingrays, Potamotrygon motoro and Potamotrygon sp., from the Amazon River basin in Brazil were investigated using integrated molecular (cytochrome c oxidase subunit 1) and cytogenetic analyses. Potamotrygon motoro presented intraspecific variation in the diploid number, with 2n=66 in the females and 2n=65 in the males, while Potamotrygon sp. had a karyotype with 66 chromosomes, in both sexes. The C-banding revealed the presence of heterochromatic blocks accumulated in the centromeric region of all the chromosomes in both species. The FISH assays with 18S DNA probes highlighted the terminal region of three or four chromosome pairs in P. motoro and seven chromosomes in Potamotrygon sp. The rDNA 5S sequences were found in only one chromosomal pair in both species. The interspecific genetic distance based on the COI sequences, between P. motoro and Potamotrygon sp. from Amazon River was 10.8%, while that between the Amazonian P. motoro and Potamotrygon amandae from the Paraná River was 2.2%, and the genetic distance between Potamotrygon sp. and P. amandae was 11.8%. In addition to the new insights on the cytogenetics of the study species, the results of the present study confirmed the existence of heteromorphic sex-linked chromosomes in P. motoro.Universidade Estadual Paulista (UNESP) Instituto de Biociências de Botucatu Laboratório de Biologia e Genética de PeixesUniversidade Federal de Mato Grosso (UFMT) Instituto de Biociências Departamento de Biologia e ZoologiaUniversidade Federal do Paraná (UFPR) Departamento de Engenharia Ambiental Laboratório de Ecologia e ConservaçãoUniversidade Federal do Ceará (UFC) Departamento de Biologia Programa de Pós-Graduação em Sistemática Uso e Conservação da BiodiversidadeUniversidade Estadual Paulista (UNESP) Instituto de Biociências de Botucatu Laboratório de Biologia e Genética de PeixesUniversidade Estadual Paulista (Unesp)Instituto de BiociênciasUniversidade Federal do Paraná (UFPR)Uso e Conservação da Biodiversidadeda Cruz, Vanessa Paes [UNESP]Nobile, Maria Ligia Oliveira [UNESP]Paim, Fabilene Gomes [UNESP]Adachi, Aisni Mayumi Correia de Lima [UNESP]Ribeiro, Giovana da Silva [UNESP]Ferreira, Daniela CarvalhoPansonato-Alves, José Carlos [UNESP]Charvet, PatríciaOliveira, Claudio [UNESP]Foresti, Fausto [UNESP]2021-06-25T10:58:14Z2021-06-25T10:58:14Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/1678-4685-gmb-2020-0083Genetics and Molecular Biology, v. 44, n. 2, 2021.1678-46851415-4757http://hdl.handle.net/11449/20761910.1590/1678-4685-gmb-2020-0083S1415-475720210003002052-s2.0-85104388733S1415-47572021000300205.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biologyinfo:eu-repo/semantics/openAccess2023-10-07T06:04:09Zoai:repositorio.unesp.br:11449/207619Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:14:39.894834Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytogenetic and molecular characteristics of potamotrygon motoro and potamotrygon sp. (chondrichthyes, myliobatiformes, potamotrygonidae) from the amazon basin: Implications for the taxonomy of the genus |
title |
Cytogenetic and molecular characteristics of potamotrygon motoro and potamotrygon sp. (chondrichthyes, myliobatiformes, potamotrygonidae) from the amazon basin: Implications for the taxonomy of the genus |
spellingShingle |
Cytogenetic and molecular characteristics of potamotrygon motoro and potamotrygon sp. (chondrichthyes, myliobatiformes, potamotrygonidae) from the amazon basin: Implications for the taxonomy of the genus da Cruz, Vanessa Paes [UNESP] Biodiversit DNA barcode Fish chromosomes Freshwater stingrays Potamotrygon |
title_short |
Cytogenetic and molecular characteristics of potamotrygon motoro and potamotrygon sp. (chondrichthyes, myliobatiformes, potamotrygonidae) from the amazon basin: Implications for the taxonomy of the genus |
title_full |
Cytogenetic and molecular characteristics of potamotrygon motoro and potamotrygon sp. (chondrichthyes, myliobatiformes, potamotrygonidae) from the amazon basin: Implications for the taxonomy of the genus |
title_fullStr |
Cytogenetic and molecular characteristics of potamotrygon motoro and potamotrygon sp. (chondrichthyes, myliobatiformes, potamotrygonidae) from the amazon basin: Implications for the taxonomy of the genus |
title_full_unstemmed |
Cytogenetic and molecular characteristics of potamotrygon motoro and potamotrygon sp. (chondrichthyes, myliobatiformes, potamotrygonidae) from the amazon basin: Implications for the taxonomy of the genus |
title_sort |
Cytogenetic and molecular characteristics of potamotrygon motoro and potamotrygon sp. (chondrichthyes, myliobatiformes, potamotrygonidae) from the amazon basin: Implications for the taxonomy of the genus |
author |
da Cruz, Vanessa Paes [UNESP] |
author_facet |
da Cruz, Vanessa Paes [UNESP] Nobile, Maria Ligia Oliveira [UNESP] Paim, Fabilene Gomes [UNESP] Adachi, Aisni Mayumi Correia de Lima [UNESP] Ribeiro, Giovana da Silva [UNESP] Ferreira, Daniela Carvalho Pansonato-Alves, José Carlos [UNESP] Charvet, Patrícia Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
author_role |
author |
author2 |
Nobile, Maria Ligia Oliveira [UNESP] Paim, Fabilene Gomes [UNESP] Adachi, Aisni Mayumi Correia de Lima [UNESP] Ribeiro, Giovana da Silva [UNESP] Ferreira, Daniela Carvalho Pansonato-Alves, José Carlos [UNESP] Charvet, Patrícia Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Instituto de Biociências Universidade Federal do Paraná (UFPR) Uso e Conservação da Biodiversidade |
dc.contributor.author.fl_str_mv |
da Cruz, Vanessa Paes [UNESP] Nobile, Maria Ligia Oliveira [UNESP] Paim, Fabilene Gomes [UNESP] Adachi, Aisni Mayumi Correia de Lima [UNESP] Ribeiro, Giovana da Silva [UNESP] Ferreira, Daniela Carvalho Pansonato-Alves, José Carlos [UNESP] Charvet, Patrícia Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
dc.subject.por.fl_str_mv |
Biodiversit DNA barcode Fish chromosomes Freshwater stingrays Potamotrygon |
topic |
Biodiversit DNA barcode Fish chromosomes Freshwater stingrays Potamotrygon |
description |
The chromosomes of two freshwater stingrays, Potamotrygon motoro and Potamotrygon sp., from the Amazon River basin in Brazil were investigated using integrated molecular (cytochrome c oxidase subunit 1) and cytogenetic analyses. Potamotrygon motoro presented intraspecific variation in the diploid number, with 2n=66 in the females and 2n=65 in the males, while Potamotrygon sp. had a karyotype with 66 chromosomes, in both sexes. The C-banding revealed the presence of heterochromatic blocks accumulated in the centromeric region of all the chromosomes in both species. The FISH assays with 18S DNA probes highlighted the terminal region of three or four chromosome pairs in P. motoro and seven chromosomes in Potamotrygon sp. The rDNA 5S sequences were found in only one chromosomal pair in both species. The interspecific genetic distance based on the COI sequences, between P. motoro and Potamotrygon sp. from Amazon River was 10.8%, while that between the Amazonian P. motoro and Potamotrygon amandae from the Paraná River was 2.2%, and the genetic distance between Potamotrygon sp. and P. amandae was 11.8%. In addition to the new insights on the cytogenetics of the study species, the results of the present study confirmed the existence of heteromorphic sex-linked chromosomes in P. motoro. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:58:14Z 2021-06-25T10:58:14Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-4685-gmb-2020-0083 Genetics and Molecular Biology, v. 44, n. 2, 2021. 1678-4685 1415-4757 http://hdl.handle.net/11449/207619 10.1590/1678-4685-gmb-2020-0083 S1415-47572021000300205 2-s2.0-85104388733 S1415-47572021000300205.pdf |
url |
http://dx.doi.org/10.1590/1678-4685-gmb-2020-0083 http://hdl.handle.net/11449/207619 |
identifier_str_mv |
Genetics and Molecular Biology, v. 44, n. 2, 2021. 1678-4685 1415-4757 10.1590/1678-4685-gmb-2020-0083 S1415-47572021000300205 2-s2.0-85104388733 S1415-47572021000300205.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128334375157760 |