Pro-inflammatory mediators expression by pulp cells following tooth whitening on restored enamel surface
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0103-6440202204688 http://hdl.handle.net/11449/240929 |
Resumo: | This paper aimed to assess the influence of adhesive restoration interface on the diffusion of hydrogen peroxide (H2O2), indirect toxicity, and pro- inflammatory mediators expression by odontoblast-like cells, after in-office tooth whitening. Dental cavities prepared in bovine enamel/dentin discs were adhesively restored and subjected or not to hydrolytic degradation (HD). A whitening gel with 35% ^CMWG) was applied for 45 min onto restored and non-restored specimens adapted to artificial pulp chambers giving rise to the groups: SD- intact discs (control); SD/HP- whitened intact discs; RT/HP- restored and whitened discs; and RT/HD/HP- restored and whitened discs subjected to HD. The extracts (culture medium + WG components diffused through enamel/dentin/restoration interface) were collected and applied to odontoblast-like MDPC-23 cells. The study evaluated the amount of H2O2 in the extracts, as well as the cell viability (CV), cell morphology (CM), and gene expression of inflammatory mediators (TNF-a and COX-2) by the pulp cells exposed to the extracts (ANOVA and Tukey tests; 5% significance). All whitened groups presented lower CV than SD (control; p<0.05). The highest CV reduction and gene expression of TNF-a and COX-2 was observed in the RT/HD/HP group in comparison with SD/HP and RT/HP (control; p<0.05). CM alterations occurred in all whitened groups. The intensity of these cell side effects was directly related with the amount of H2O2 in the extracts. We concluded that adhesive restoration of dental cavity increases the H2O2 diffusion after in-office whitening, enhancing the indirect toxicity of this therapy and trigger pro-inflammatory overexpression by MDPC-23 cells. |
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Pro-inflammatory mediators expression by pulp cells following tooth whitening on restored enamel surfaceAdhesionInflammationOdontoblastsTooth-bleachingToxicityThis paper aimed to assess the influence of adhesive restoration interface on the diffusion of hydrogen peroxide (H2O2), indirect toxicity, and pro- inflammatory mediators expression by odontoblast-like cells, after in-office tooth whitening. Dental cavities prepared in bovine enamel/dentin discs were adhesively restored and subjected or not to hydrolytic degradation (HD). A whitening gel with 35% ^CMWG) was applied for 45 min onto restored and non-restored specimens adapted to artificial pulp chambers giving rise to the groups: SD- intact discs (control); SD/HP- whitened intact discs; RT/HP- restored and whitened discs; and RT/HD/HP- restored and whitened discs subjected to HD. The extracts (culture medium + WG components diffused through enamel/dentin/restoration interface) were collected and applied to odontoblast-like MDPC-23 cells. The study evaluated the amount of H2O2 in the extracts, as well as the cell viability (CV), cell morphology (CM), and gene expression of inflammatory mediators (TNF-a and COX-2) by the pulp cells exposed to the extracts (ANOVA and Tukey tests; 5% significance). All whitened groups presented lower CV than SD (control; p<0.05). The highest CV reduction and gene expression of TNF-a and COX-2 was observed in the RT/HD/HP group in comparison with SD/HP and RT/HP (control; p<0.05). CM alterations occurred in all whitened groups. The intensity of these cell side effects was directly related with the amount of H2O2 in the extracts. We concluded that adhesive restoration of dental cavity increases the H2O2 diffusion after in-office whitening, enhancing the indirect toxicity of this therapy and trigger pro-inflammatory overexpression by MDPC-23 cells.Endodontics and Dental Materials Bauru School of Dentistry University of Sao Paulo, SPDepartment of Pediatric Dentistry Unip - Universidade Paulista, Br 153 Highway, Km 503, area 1-5, s/n - Fazenda BotafogoDepartment of Restorative Dental Sciences College of Dentistry University of Florida, 1395 Center DriveDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry Universidade Estadual PaulistaDepartment of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry Sao Paulo State UniversityDepartment of Physiology and Pathology Sao Paulo State University (UNESP) School of DentistryDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry Universidade Estadual PaulistaDepartment of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry Sao Paulo State UniversityDepartment of Physiology and Pathology Sao Paulo State University (UNESP) School of DentistryUniversidade de São Paulo (USP)Unip - Universidade PaulistaUniversity of FloridaUniversidade Estadual Paulista (UNESP)Soares, Diana GabrielaSacono, Nancy TomokoRibeiro, Ana Paula DiasLeite, Maria Luisa [UNESP]Duque, Carla Caroline de Oliveira [UNESP]Gallinari, Marjorie De OliveiraPacheco, Leandro EdgarHebling, Josimeri [UNESP]Costa, Carlos Alberto de Souza [UNESP]2023-03-01T20:39:07Z2023-03-01T20:39:07Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article83-90http://dx.doi.org/10.1590/0103-6440202204688Brazilian Dental Journal, v. 33, n. 2, p. 83-90, 2022.1806-47600103-6440http://hdl.handle.net/11449/24092910.1590/0103-64402022046882-s2.0-85129393366Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journalinfo:eu-repo/semantics/openAccess2023-03-01T20:39:07Zoai:repositorio.unesp.br:11449/240929Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T20:39:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Pro-inflammatory mediators expression by pulp cells following tooth whitening on restored enamel surface |
title |
Pro-inflammatory mediators expression by pulp cells following tooth whitening on restored enamel surface |
spellingShingle |
Pro-inflammatory mediators expression by pulp cells following tooth whitening on restored enamel surface Soares, Diana Gabriela Adhesion Inflammation Odontoblasts Tooth-bleaching Toxicity |
title_short |
Pro-inflammatory mediators expression by pulp cells following tooth whitening on restored enamel surface |
title_full |
Pro-inflammatory mediators expression by pulp cells following tooth whitening on restored enamel surface |
title_fullStr |
Pro-inflammatory mediators expression by pulp cells following tooth whitening on restored enamel surface |
title_full_unstemmed |
Pro-inflammatory mediators expression by pulp cells following tooth whitening on restored enamel surface |
title_sort |
Pro-inflammatory mediators expression by pulp cells following tooth whitening on restored enamel surface |
author |
Soares, Diana Gabriela |
author_facet |
Soares, Diana Gabriela Sacono, Nancy Tomoko Ribeiro, Ana Paula Dias Leite, Maria Luisa [UNESP] Duque, Carla Caroline de Oliveira [UNESP] Gallinari, Marjorie De Oliveira Pacheco, Leandro Edgar Hebling, Josimeri [UNESP] Costa, Carlos Alberto de Souza [UNESP] |
author_role |
author |
author2 |
Sacono, Nancy Tomoko Ribeiro, Ana Paula Dias Leite, Maria Luisa [UNESP] Duque, Carla Caroline de Oliveira [UNESP] Gallinari, Marjorie De Oliveira Pacheco, Leandro Edgar Hebling, Josimeri [UNESP] Costa, Carlos Alberto de Souza [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Unip - Universidade Paulista University of Florida Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Soares, Diana Gabriela Sacono, Nancy Tomoko Ribeiro, Ana Paula Dias Leite, Maria Luisa [UNESP] Duque, Carla Caroline de Oliveira [UNESP] Gallinari, Marjorie De Oliveira Pacheco, Leandro Edgar Hebling, Josimeri [UNESP] Costa, Carlos Alberto de Souza [UNESP] |
dc.subject.por.fl_str_mv |
Adhesion Inflammation Odontoblasts Tooth-bleaching Toxicity |
topic |
Adhesion Inflammation Odontoblasts Tooth-bleaching Toxicity |
description |
This paper aimed to assess the influence of adhesive restoration interface on the diffusion of hydrogen peroxide (H2O2), indirect toxicity, and pro- inflammatory mediators expression by odontoblast-like cells, after in-office tooth whitening. Dental cavities prepared in bovine enamel/dentin discs were adhesively restored and subjected or not to hydrolytic degradation (HD). A whitening gel with 35% ^CMWG) was applied for 45 min onto restored and non-restored specimens adapted to artificial pulp chambers giving rise to the groups: SD- intact discs (control); SD/HP- whitened intact discs; RT/HP- restored and whitened discs; and RT/HD/HP- restored and whitened discs subjected to HD. The extracts (culture medium + WG components diffused through enamel/dentin/restoration interface) were collected and applied to odontoblast-like MDPC-23 cells. The study evaluated the amount of H2O2 in the extracts, as well as the cell viability (CV), cell morphology (CM), and gene expression of inflammatory mediators (TNF-a and COX-2) by the pulp cells exposed to the extracts (ANOVA and Tukey tests; 5% significance). All whitened groups presented lower CV than SD (control; p<0.05). The highest CV reduction and gene expression of TNF-a and COX-2 was observed in the RT/HD/HP group in comparison with SD/HP and RT/HP (control; p<0.05). CM alterations occurred in all whitened groups. The intensity of these cell side effects was directly related with the amount of H2O2 in the extracts. We concluded that adhesive restoration of dental cavity increases the H2O2 diffusion after in-office whitening, enhancing the indirect toxicity of this therapy and trigger pro-inflammatory overexpression by MDPC-23 cells. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-01T20:39:07Z 2023-03-01T20:39:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0103-6440202204688 Brazilian Dental Journal, v. 33, n. 2, p. 83-90, 2022. 1806-4760 0103-6440 http://hdl.handle.net/11449/240929 10.1590/0103-6440202204688 2-s2.0-85129393366 |
url |
http://dx.doi.org/10.1590/0103-6440202204688 http://hdl.handle.net/11449/240929 |
identifier_str_mv |
Brazilian Dental Journal, v. 33, n. 2, p. 83-90, 2022. 1806-4760 0103-6440 10.1590/0103-6440202204688 2-s2.0-85129393366 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
83-90 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964591016378368 |