Treadmill Training Increases SIRT-1 and PGC-1 alpha Protein Levels and AMPK Phosphorylation in Quadriceps of Middle-Aged Rats in an Intensity-Dependent Manner

• Autor(a) principal: Oliveira, Nara R. C.
• Data de Publicação: 2014
• Outros Autores: Marques, Scherolin O., Luciano, Thais F., Pauli, Jose R. [UNESP], Moura, Leandro P. [UNESP], Caperuto, Erico, Pieri, Bruno L. S., Engelmann, Julia, Scaini, Gisele, Streck, Emilio L., Lira, Fabio S. [UNESP], Pinho, Ricardo A., Ropelle, Eduardo R., Silva, Adelino S. R., De Souza, Claudio T.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1155/2014/987017; http://hdl.handle.net/11449/113380

The present study investigated the effects of running at 0.8 or 1.2 km/h on inflammatory proteins (i.e., protein levels of TNF-alpha, IL-1 beta, and NF-kappa B) and metabolic proteins (i.e., protein levels of SIRT-1 and PGC-1 alpha, and AMPK phosphorylation) in quadriceps of rats. Male Wistar rats at 3 (young) and 18 months (middle-aged rats) of age were divided into nonexercised (NE) and exercised at 0.8 or 1.2 km/h. The rats were trained on treadmill, 50 min per day, 5 days per week, during 8 weeks. Forty-eight hours after the last training session, muscles were removed, homogenized, and analyzed using biochemical and western blot techniques. Our results showed that: (a) running at 0.8 km/h decreased the inflammatory proteins and increased the metabolic proteins compared with NE rats; (b) these responses were lower for the inflammatory proteins and higher for the metabolic proteins in young rats compared with middle-aged rats; (c) running at 1.2 km/h decreased the inflammatory proteins and increased the metabolic proteins compared with 0.8 km/h; (d) these responses were similar between young and middle-aged rats when trained at 1.2 km. In summary, the age-related increases in inflammatory proteins, and the age-related declines in metabolic proteins can be reversed and largely improved by treadmill training.