Multi-locus Sequencing Typing of Bartonella henselae isolates reveals coinfection with different variants in domestic cats from Midwestern Brazil

Detalhes bibliográficos
Autor(a) principal: Dias, Clara Morato [UNESP]
Data de Publicação: 2023
Outros Autores: do Amaral, Renan Bressianini [UNESP], Perles, Lívia [UNESP], Muniz, Antônia Laila dos Santos, Rocha, Tarik Fernandes Gonçalves, Machado, Rosangela Zacarias [UNESP], André, Marcos Rogério [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.actatropica.2022.106742
http://hdl.handle.net/11449/247834
Resumo: Bartonella henselae is a zoonotic pathogen responsible for causing Cat Scratch Disease (CSD) and other clinical manifestations in humans. Domestic cats are the main reservoirs of this Bartonella species. Previous studies have suggested that certain genotypes of B. henselae seem to be more associated with human infections. The present study aimed to genotype B. henselae isolates from domestic cats’ blood samples in the state of Goiás, midwestern Brazil. The association of quantitative real-time PCR (qPCR) based on the nuoG gene from Bartonella spp. of blood samples, before and after incubation in pre-enrichment liquid medium (BAPGM) and isolation on chocolate agar, showed a positivity frequency of 42% (42/100) for Bartonella spp. Twelve B. henselae isolates obtained on agar chocolate from six cats’ blood samples (two isolates from each animal) were characterized by Multi-locus Sequencing Typing (MLST) and revealed to belong to Sequence Types ST1 and ST5. One of the cats (1/6) presented both STs, demonstrating that domestic cats can be coinfected with different variants of B. henselae. The STs detected in this study are distributed worldwide and have already been detected in humans with clinical manifestations of bartonellosis. This is the first report of the zoonotic variants ST1 and ST5 of B. henselae in domestic cats from Brazil.
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spelling Multi-locus Sequencing Typing of Bartonella henselae isolates reveals coinfection with different variants in domestic cats from Midwestern BrazilBartonellosisCAT Scratch DiseaseMLSTSequence TypeBartonella henselae is a zoonotic pathogen responsible for causing Cat Scratch Disease (CSD) and other clinical manifestations in humans. Domestic cats are the main reservoirs of this Bartonella species. Previous studies have suggested that certain genotypes of B. henselae seem to be more associated with human infections. The present study aimed to genotype B. henselae isolates from domestic cats’ blood samples in the state of Goiás, midwestern Brazil. The association of quantitative real-time PCR (qPCR) based on the nuoG gene from Bartonella spp. of blood samples, before and after incubation in pre-enrichment liquid medium (BAPGM) and isolation on chocolate agar, showed a positivity frequency of 42% (42/100) for Bartonella spp. Twelve B. henselae isolates obtained on agar chocolate from six cats’ blood samples (two isolates from each animal) were characterized by Multi-locus Sequencing Typing (MLST) and revealed to belong to Sequence Types ST1 and ST5. One of the cats (1/6) presented both STs, demonstrating that domestic cats can be coinfected with different variants of B. henselae. The STs detected in this study are distributed worldwide and have already been detected in humans with clinical manifestations of bartonellosis. This is the first report of the zoonotic variants ST1 and ST5 of B. henselae in domestic cats from Brazil.Departamento de Patologia Reprodução e Saúde Única Laboratório de Imunoparasitologia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual “Júlio de Mesquita Filho” (FCAV/UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona Rural, CEP:, São PauloEscola de Veterinária e Zootecnia Universidade Federal de Goiás (UFG), GoiásDepartamento de Patologia Reprodução e Saúde Única Laboratório de Imunoparasitologia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual “Júlio de Mesquita Filho” (FCAV/UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona Rural, CEP:, São PauloUniversidade Estadual Paulista (UNESP)Universidade Federal de Goiás (UFG)Dias, Clara Morato [UNESP]do Amaral, Renan Bressianini [UNESP]Perles, Lívia [UNESP]Muniz, Antônia Laila dos SantosRocha, Tarik Fernandes GonçalvesMachado, Rosangela Zacarias [UNESP]André, Marcos Rogério [UNESP]2023-07-29T13:27:11Z2023-07-29T13:27:11Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.actatropica.2022.106742Acta Tropica, v. 237.1873-62540001-706Xhttp://hdl.handle.net/11449/24783410.1016/j.actatropica.2022.1067422-s2.0-85141240255Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Tropicainfo:eu-repo/semantics/openAccess2024-06-07T13:02:48Zoai:repositorio.unesp.br:11449/247834Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:17:05.526430Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Multi-locus Sequencing Typing of Bartonella henselae isolates reveals coinfection with different variants in domestic cats from Midwestern Brazil
title Multi-locus Sequencing Typing of Bartonella henselae isolates reveals coinfection with different variants in domestic cats from Midwestern Brazil
spellingShingle Multi-locus Sequencing Typing of Bartonella henselae isolates reveals coinfection with different variants in domestic cats from Midwestern Brazil
Dias, Clara Morato [UNESP]
Bartonellosis
CAT Scratch Disease
MLST
Sequence Type
title_short Multi-locus Sequencing Typing of Bartonella henselae isolates reveals coinfection with different variants in domestic cats from Midwestern Brazil
title_full Multi-locus Sequencing Typing of Bartonella henselae isolates reveals coinfection with different variants in domestic cats from Midwestern Brazil
title_fullStr Multi-locus Sequencing Typing of Bartonella henselae isolates reveals coinfection with different variants in domestic cats from Midwestern Brazil
title_full_unstemmed Multi-locus Sequencing Typing of Bartonella henselae isolates reveals coinfection with different variants in domestic cats from Midwestern Brazil
title_sort Multi-locus Sequencing Typing of Bartonella henselae isolates reveals coinfection with different variants in domestic cats from Midwestern Brazil
author Dias, Clara Morato [UNESP]
author_facet Dias, Clara Morato [UNESP]
do Amaral, Renan Bressianini [UNESP]
Perles, Lívia [UNESP]
Muniz, Antônia Laila dos Santos
Rocha, Tarik Fernandes Gonçalves
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
author_role author
author2 do Amaral, Renan Bressianini [UNESP]
Perles, Lívia [UNESP]
Muniz, Antônia Laila dos Santos
Rocha, Tarik Fernandes Gonçalves
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de Goiás (UFG)
dc.contributor.author.fl_str_mv Dias, Clara Morato [UNESP]
do Amaral, Renan Bressianini [UNESP]
Perles, Lívia [UNESP]
Muniz, Antônia Laila dos Santos
Rocha, Tarik Fernandes Gonçalves
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
dc.subject.por.fl_str_mv Bartonellosis
CAT Scratch Disease
MLST
Sequence Type
topic Bartonellosis
CAT Scratch Disease
MLST
Sequence Type
description Bartonella henselae is a zoonotic pathogen responsible for causing Cat Scratch Disease (CSD) and other clinical manifestations in humans. Domestic cats are the main reservoirs of this Bartonella species. Previous studies have suggested that certain genotypes of B. henselae seem to be more associated with human infections. The present study aimed to genotype B. henselae isolates from domestic cats’ blood samples in the state of Goiás, midwestern Brazil. The association of quantitative real-time PCR (qPCR) based on the nuoG gene from Bartonella spp. of blood samples, before and after incubation in pre-enrichment liquid medium (BAPGM) and isolation on chocolate agar, showed a positivity frequency of 42% (42/100) for Bartonella spp. Twelve B. henselae isolates obtained on agar chocolate from six cats’ blood samples (two isolates from each animal) were characterized by Multi-locus Sequencing Typing (MLST) and revealed to belong to Sequence Types ST1 and ST5. One of the cats (1/6) presented both STs, demonstrating that domestic cats can be coinfected with different variants of B. henselae. The STs detected in this study are distributed worldwide and have already been detected in humans with clinical manifestations of bartonellosis. This is the first report of the zoonotic variants ST1 and ST5 of B. henselae in domestic cats from Brazil.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:27:11Z
2023-07-29T13:27:11Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.actatropica.2022.106742
Acta Tropica, v. 237.
1873-6254
0001-706X
http://hdl.handle.net/11449/247834
10.1016/j.actatropica.2022.106742
2-s2.0-85141240255
url http://dx.doi.org/10.1016/j.actatropica.2022.106742
http://hdl.handle.net/11449/247834
identifier_str_mv Acta Tropica, v. 237.
1873-6254
0001-706X
10.1016/j.actatropica.2022.106742
2-s2.0-85141240255
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Tropica
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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