Cyclodextrin glycosyltransferase from Bacillus licheniformis: Optimization of production and its properties
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1517-83822006000300022 http://hdl.handle.net/11449/7567 |
Resumo: | Cyclodextrin glycosyltransferase (EC 2.4.1.19) is an enzyme that produces cyclodextrins from starch via an intramolecular transglycosylation reaction. An alkalophilic Bacillus strain, isolated from cassava peels, was identified as Bacillus licheniformis. CGTase production by this strain was better when potato starch was used as carbon source, followed by cassava starch and amylopectin. Glucose and amylose, on the other hand, acted as synthesis repressors. When the cultivation was supplemented with sodium ions and had the pH adjusted between 6.0 and 9.0, the microorganism maintained the growth and enzyme production capacity. This data is interesting because it contradicts the concept that alkalophilic microorganisms do not grow in this pH range. After ultrafiltration-centrifugation, one protein of 85.2 kDa with CGTase activity was isolated. This protein was identified in plates with starch and phenolphthalein. Determination of the optimum temperature showed higher activities at 25 degrees C and 55 degrees C, indicating the possible presence of more than one CGTase in the culture filtrate. Km and Vmax values were 1.77 mg/mL and 0.0263 U/mg protein, respectively, using potato starch as substrate. |
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Cyclodextrin glycosyltransferase from Bacillus licheniformis: Optimization of production and its propertiescyclodextrin glycosyltransferaseCGTase productionBacillus licheniformisBacillus beta-CDCyclodextrin glycosyltransferase (EC 2.4.1.19) is an enzyme that produces cyclodextrins from starch via an intramolecular transglycosylation reaction. An alkalophilic Bacillus strain, isolated from cassava peels, was identified as Bacillus licheniformis. CGTase production by this strain was better when potato starch was used as carbon source, followed by cassava starch and amylopectin. Glucose and amylose, on the other hand, acted as synthesis repressors. When the cultivation was supplemented with sodium ions and had the pH adjusted between 6.0 and 9.0, the microorganism maintained the growth and enzyme production capacity. This data is interesting because it contradicts the concept that alkalophilic microorganisms do not grow in this pH range. After ultrafiltration-centrifugation, one protein of 85.2 kDa with CGTase activity was isolated. This protein was identified in plates with starch and phenolphthalein. Determination of the optimum temperature showed higher activities at 25 degrees C and 55 degrees C, indicating the possible presence of more than one CGTase in the culture filtrate. Km and Vmax values were 1.77 mg/mL and 0.0263 U/mg protein, respectively, using potato starch as substrate.Univ Estadual Paulista, Inst Quim, Dept Bioquim & Quim Tecnol, Araraquara, SP, BrazilUniv Estadual Paulista, Fac Ciências Farmaceut, Dept Alimentos & Nutr, Araraquara, SP, BrazilUniv Estadual Paulista, Inst Quim, Dept Bioquim & Quim Tecnol, Araraquara, SP, BrazilUniv Estadual Paulista, Fac Ciências Farmaceut, Dept Alimentos & Nutr, Araraquara, SP, BrazilSoc Brasileira MicrobiologiaUniversidade Estadual Paulista (Unesp)Martins Bonilha, Paulo RobertoMenocci, VivianGoulart, Antonio JoseTeixeira de Moraes Polizeli, Maria de LourdesMonti, Rubens [UNESP]2014-05-20T13:24:25Z2014-05-20T13:24:25Z2006-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article317-323application/pdfhttp://dx.doi.org/10.1590/S1517-83822006000300022Brazilian Journal of Microbiology. São Paulo: Soc Brasileira Microbiologia, v. 37, n. 3, p. 317-323, 2006.1517-8382http://hdl.handle.net/11449/756710.1590/S1517-83822006000300022S1517-83822006000300022WOS:000243335200022WOS000243335200022.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiology1.8100,630info:eu-repo/semantics/openAccess2024-06-21T12:47:23Zoai:repositorio.unesp.br:11449/7567Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:38:08.701329Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: Optimization of production and its properties |
title |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: Optimization of production and its properties |
spellingShingle |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: Optimization of production and its properties Martins Bonilha, Paulo Roberto cyclodextrin glycosyltransferase CGTase production Bacillus licheniformis Bacillus beta-CD |
title_short |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: Optimization of production and its properties |
title_full |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: Optimization of production and its properties |
title_fullStr |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: Optimization of production and its properties |
title_full_unstemmed |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: Optimization of production and its properties |
title_sort |
Cyclodextrin glycosyltransferase from Bacillus licheniformis: Optimization of production and its properties |
author |
Martins Bonilha, Paulo Roberto |
author_facet |
Martins Bonilha, Paulo Roberto Menocci, Vivian Goulart, Antonio Jose Teixeira de Moraes Polizeli, Maria de Lourdes Monti, Rubens [UNESP] |
author_role |
author |
author2 |
Menocci, Vivian Goulart, Antonio Jose Teixeira de Moraes Polizeli, Maria de Lourdes Monti, Rubens [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Martins Bonilha, Paulo Roberto Menocci, Vivian Goulart, Antonio Jose Teixeira de Moraes Polizeli, Maria de Lourdes Monti, Rubens [UNESP] |
dc.subject.por.fl_str_mv |
cyclodextrin glycosyltransferase CGTase production Bacillus licheniformis Bacillus beta-CD |
topic |
cyclodextrin glycosyltransferase CGTase production Bacillus licheniformis Bacillus beta-CD |
description |
Cyclodextrin glycosyltransferase (EC 2.4.1.19) is an enzyme that produces cyclodextrins from starch via an intramolecular transglycosylation reaction. An alkalophilic Bacillus strain, isolated from cassava peels, was identified as Bacillus licheniformis. CGTase production by this strain was better when potato starch was used as carbon source, followed by cassava starch and amylopectin. Glucose and amylose, on the other hand, acted as synthesis repressors. When the cultivation was supplemented with sodium ions and had the pH adjusted between 6.0 and 9.0, the microorganism maintained the growth and enzyme production capacity. This data is interesting because it contradicts the concept that alkalophilic microorganisms do not grow in this pH range. After ultrafiltration-centrifugation, one protein of 85.2 kDa with CGTase activity was isolated. This protein was identified in plates with starch and phenolphthalein. Determination of the optimum temperature showed higher activities at 25 degrees C and 55 degrees C, indicating the possible presence of more than one CGTase in the culture filtrate. Km and Vmax values were 1.77 mg/mL and 0.0263 U/mg protein, respectively, using potato starch as substrate. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-07-01 2014-05-20T13:24:25Z 2014-05-20T13:24:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1517-83822006000300022 Brazilian Journal of Microbiology. São Paulo: Soc Brasileira Microbiologia, v. 37, n. 3, p. 317-323, 2006. 1517-8382 http://hdl.handle.net/11449/7567 10.1590/S1517-83822006000300022 S1517-83822006000300022 WOS:000243335200022 WOS000243335200022.pdf |
url |
http://dx.doi.org/10.1590/S1517-83822006000300022 http://hdl.handle.net/11449/7567 |
identifier_str_mv |
Brazilian Journal of Microbiology. São Paulo: Soc Brasileira Microbiologia, v. 37, n. 3, p. 317-323, 2006. 1517-8382 10.1590/S1517-83822006000300022 S1517-83822006000300022 WOS:000243335200022 WOS000243335200022.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Microbiology 1.810 0,630 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
317-323 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasileira Microbiologia |
publisher.none.fl_str_mv |
Soc Brasileira Microbiologia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129538802057216 |