Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats

Detalhes bibliográficos
Autor(a) principal: Pinotti, Matheus Fécchio
Data de Publicação: 2018
Outros Autores: Matias, Amanda Martins, Sugizaki, Mário Mateus, Nascimento, André Ferreira Do, Pai, Maeli Dal, Leopoldo, Ana Paula Lima, Cicogna, Antônio Carlos, Leopoldo, André Soares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.5935/abc.20180152
http://hdl.handle.net/11449/158097
Resumo: AbstractBackground: Caloric restriction is known to impair the cardiac function and morphology in hypertrophied hearts of spontaneously hypertensive rats (SHR); however, the influence of fasting/refeeding (RF) is unknown.Objective: To investigate the fasting/refeeding approach on myocardial remodeling and function. In addition, the current study was designed to bring information regarding the mechanisms underlying the participation of Ca2+ handling and b-adrenergic system.Methods: Sixty-day-old male SHR rats were submitted to food ad libitum (C), 50% food restriction (R50) or RF cycles for 90 days. Cardiac remodeling was assessed by ultrastructure analysis and isolated papillary muscle function. The level of significance considered was 5% (a = 0.05).Results: The RF rats presented lower cardiac atrophy than R50 in relation to C rats. The C rats increased weight gain, R50 maintained their initial body weight and RF rats increased and decreased weight during RF. The RF did not cause functional impairment because the isotonic and isometric parameters showed similar behavior to those of C. The isotonic and isometric cardiac parameters were significantly elevated in RF rats compared to R50 rats. In addition, the R50 rats had cardiac damage in relation to C for isotonic and isometric variables. While the R50 rats showed focal changes in many muscle fibers, the RF rats displayed mild alterations, such as loss or disorganization of myofibrils.Conclusion: Fasting/refeeding promotes cardiac beneficial effects and attenuates myocardial injury caused by caloric restriction in SHR rats, contributing to reduce the cardiovascular risk profile and morphological injuries. Furthermore, RF promotes mild improvement in Ca2+ handling and b-adrenergic system.
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spelling Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive RatsCiclos de Jejum/Realimentação Previnem Disfunção Miocárdica e Danos Morfológicos em Ratos Espontaneamente HipertensosRatsHypertensionMyocardial/dysfunctionChronic DiseaseFasting, ReffedingCaloric RestrictionRatosHipertensãoMiocárdio/disfunçãoDoença CrônicaRealimentaçãoRestrição CalóricaAbstractBackground: Caloric restriction is known to impair the cardiac function and morphology in hypertrophied hearts of spontaneously hypertensive rats (SHR); however, the influence of fasting/refeeding (RF) is unknown.Objective: To investigate the fasting/refeeding approach on myocardial remodeling and function. In addition, the current study was designed to bring information regarding the mechanisms underlying the participation of Ca2+ handling and b-adrenergic system.Methods: Sixty-day-old male SHR rats were submitted to food ad libitum (C), 50% food restriction (R50) or RF cycles for 90 days. Cardiac remodeling was assessed by ultrastructure analysis and isolated papillary muscle function. The level of significance considered was 5% (a = 0.05).Results: The RF rats presented lower cardiac atrophy than R50 in relation to C rats. The C rats increased weight gain, R50 maintained their initial body weight and RF rats increased and decreased weight during RF. The RF did not cause functional impairment because the isotonic and isometric parameters showed similar behavior to those of C. The isotonic and isometric cardiac parameters were significantly elevated in RF rats compared to R50 rats. In addition, the R50 rats had cardiac damage in relation to C for isotonic and isometric variables. While the R50 rats showed focal changes in many muscle fibers, the RF rats displayed mild alterations, such as loss or disorganization of myofibrils.Conclusion: Fasting/refeeding promotes cardiac beneficial effects and attenuates myocardial injury caused by caloric restriction in SHR rats, contributing to reduce the cardiovascular risk profile and morphological injuries. Furthermore, RF promotes mild improvement in Ca2+ handling and b-adrenergic system.ResumoFundamento: A restrição calórica compromete a função e a morfologia cardíacas em corações hipertrofiados de ratos espontaneamente hipertensos (SHR). No entanto, a influência de ciclo de jejum/Realimentação é desconhecida.Objetivo: Investigar o efeito de ciclos de jejum/realimentação sobre a remodelação e função miocárdica. Além disso, o presente estudo foi desenhado para avaliar os mecanismos subjacentes à participação do trânsito de cálcio (Ca+2) e sistema beta-adrenérgico.Métodos: Neste estudo, SHR machos de 60 dias de idade foram submetidos a alimento ad libitum (grupo C), 50% de restrição alimentar (grupo R50) ou ciclos de RF (grupo RF) por 90 dias. A remodelação cardíaca foi avaliada por meio da análise ultraestrutural e função do músculo papilar isolado. Adotou-se o nível de significância de 5% (a = 0,05).Resultados: Os ratos do grupo RF apresentaram menor atrofia cardíaca do que os do grupo R50 em relação aos do grupo C. Os ratos do grupo C aumentaram peso corporal, os ratos do grupo R50 mantiveram seu peso corporal inicial e os ratos do grupo RF aumentaram e reduziram seu peso durante o ciclo RF. O ciclo RF não causou comprometimento funcional, pois os parâmetros isotônicos e isométricos apresentaram comportamento similar aos dos ratos do grupo C. Os parâmetros cardíacos isotônicos e isométricos mostraram-se significativamente elevados nos ratos do grupo RF em comparação aos dos ratos do grupo R50. Além disso, os ratos do grupo R50 apresentaram dano cardíaco em comparação aos ratos do grupo C quanto às variáveis isotônicas e isométricas. Os ratos do grupo R50 apresentaram alterações focais em muitas fibras musculares, enquanto os ratos do grupo RF apresentaram leves alterações, como perda ou desorganização de miofibrilas.Conclusão: Ciclos de Jejum/Realimentação promovem efeitos benéficos cardíacos e atenuam o dano miocárdico causado por restrição calórica em SHR, contribuindo para reduzir o risco cardiovascular e os danos morfológicos. Além disso, o ciclo de jejum/realimentação promove leve melhora do trânsito do Ca2+ e do sistema beta-adrenérgico.Universidade Estadual Paulista Faculdade de Medicina Departamento de Clínica MédicaUniversidade Federal do Espírito Santo Centro de Educação Física e Desportos Departamento de DesportosUniversidade Federal de Mato GrossoUniversidade Estadual Paulista Instituto de Biosciências Departamento de MorfologiaUniversidade Estadual Paulista Faculdade de Medicina Departamento de Clínica MédicaUniversidade Estadual Paulista Instituto de Biosciências Departamento de MorfologiaSociedade Brasileira de Cardiologia - SBCUniversidade Estadual Paulista (Unesp)Universidade Federal do Espírito Santo Centro de Educação Física e Desportos Departamento de DesportosUniversidade Federal de Mato GrossoPinotti, Matheus FécchioMatias, Amanda MartinsSugizaki, Mário MateusNascimento, André Ferreira DoPai, Maeli DalLeopoldo, Ana Paula LimaCicogna, Antônio CarlosLeopoldo, André Soares2018-11-12T17:28:18Z2018-11-12T17:28:18Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.5935/abc.20180152Arquivos Brasileiros de Cardiologia. Sociedade Brasileira de Cardiologia - SBC, n. ahead, p. -, 2018.0066-782Xhttp://hdl.handle.net/11449/15809710.5935/abc.20180152S0066-782X2018005011103S0066-782X2018005011103.pdf6171323341671821SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivos Brasileiros de Cardiologiainfo:eu-repo/semantics/openAccess2024-08-14T17:22:36Zoai:repositorio.unesp.br:11449/158097Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats
Ciclos de Jejum/Realimentação Previnem Disfunção Miocárdica e Danos Morfológicos em Ratos Espontaneamente Hipertensos
title Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats
spellingShingle Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats
Pinotti, Matheus Fécchio
Rats
Hypertension
Myocardial/dysfunction
Chronic Disease
Fasting, Reffeding
Caloric Restriction
Ratos
Hipertensão
Miocárdio/disfunção
Doença Crônica
Realimentação
Restrição Calórica
title_short Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats
title_full Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats
title_fullStr Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats
title_full_unstemmed Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats
title_sort Fasting/Refeeding Cycles Prevent Myocardial Dysfunction and Morphology Damage in the Spontaneously Hypertensive Rats
author Pinotti, Matheus Fécchio
author_facet Pinotti, Matheus Fécchio
Matias, Amanda Martins
Sugizaki, Mário Mateus
Nascimento, André Ferreira Do
Pai, Maeli Dal
Leopoldo, Ana Paula Lima
Cicogna, Antônio Carlos
Leopoldo, André Soares
author_role author
author2 Matias, Amanda Martins
Sugizaki, Mário Mateus
Nascimento, André Ferreira Do
Pai, Maeli Dal
Leopoldo, Ana Paula Lima
Cicogna, Antônio Carlos
Leopoldo, André Soares
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal do Espírito Santo Centro de Educação Física e Desportos Departamento de Desportos
Universidade Federal de Mato Grosso
dc.contributor.author.fl_str_mv Pinotti, Matheus Fécchio
Matias, Amanda Martins
Sugizaki, Mário Mateus
Nascimento, André Ferreira Do
Pai, Maeli Dal
Leopoldo, Ana Paula Lima
Cicogna, Antônio Carlos
Leopoldo, André Soares
dc.subject.por.fl_str_mv Rats
Hypertension
Myocardial/dysfunction
Chronic Disease
Fasting, Reffeding
Caloric Restriction
Ratos
Hipertensão
Miocárdio/disfunção
Doença Crônica
Realimentação
Restrição Calórica
topic Rats
Hypertension
Myocardial/dysfunction
Chronic Disease
Fasting, Reffeding
Caloric Restriction
Ratos
Hipertensão
Miocárdio/disfunção
Doença Crônica
Realimentação
Restrição Calórica
description AbstractBackground: Caloric restriction is known to impair the cardiac function and morphology in hypertrophied hearts of spontaneously hypertensive rats (SHR); however, the influence of fasting/refeeding (RF) is unknown.Objective: To investigate the fasting/refeeding approach on myocardial remodeling and function. In addition, the current study was designed to bring information regarding the mechanisms underlying the participation of Ca2+ handling and b-adrenergic system.Methods: Sixty-day-old male SHR rats were submitted to food ad libitum (C), 50% food restriction (R50) or RF cycles for 90 days. Cardiac remodeling was assessed by ultrastructure analysis and isolated papillary muscle function. The level of significance considered was 5% (a = 0.05).Results: The RF rats presented lower cardiac atrophy than R50 in relation to C rats. The C rats increased weight gain, R50 maintained their initial body weight and RF rats increased and decreased weight during RF. The RF did not cause functional impairment because the isotonic and isometric parameters showed similar behavior to those of C. The isotonic and isometric cardiac parameters were significantly elevated in RF rats compared to R50 rats. In addition, the R50 rats had cardiac damage in relation to C for isotonic and isometric variables. While the R50 rats showed focal changes in many muscle fibers, the RF rats displayed mild alterations, such as loss or disorganization of myofibrils.Conclusion: Fasting/refeeding promotes cardiac beneficial effects and attenuates myocardial injury caused by caloric restriction in SHR rats, contributing to reduce the cardiovascular risk profile and morphological injuries. Furthermore, RF promotes mild improvement in Ca2+ handling and b-adrenergic system.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-12T17:28:18Z
2018-11-12T17:28:18Z
2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5935/abc.20180152
Arquivos Brasileiros de Cardiologia. Sociedade Brasileira de Cardiologia - SBC, n. ahead, p. -, 2018.
0066-782X
http://hdl.handle.net/11449/158097
10.5935/abc.20180152
S0066-782X2018005011103
S0066-782X2018005011103.pdf
6171323341671821
url http://dx.doi.org/10.5935/abc.20180152
http://hdl.handle.net/11449/158097
identifier_str_mv Arquivos Brasileiros de Cardiologia. Sociedade Brasileira de Cardiologia - SBC, n. ahead, p. -, 2018.
0066-782X
10.5935/abc.20180152
S0066-782X2018005011103
S0066-782X2018005011103.pdf
6171323341671821
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos Brasileiros de Cardiologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Cardiologia - SBC
publisher.none.fl_str_mv Sociedade Brasileira de Cardiologia - SBC
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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