Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids

Detalhes bibliográficos
Autor(a) principal: Teixeira, Weslen Fabricio Pires [UNESP]
Data de Publicação: 2021
Outros Autores: De Oliveira, Márcio Leite [UNESP], de Faria Peres, Pedro Henrique [UNESP], Nagata, Walter Bertequini [UNESP], Santana, Bruna Nicoleti [UNESP], Oliveira, Bruno César Miranda [UNESP], Duarte, José Maurício Barbanti [UNESP], Cardoso, Tereza Cristina [UNESP], Lopes, Welber Daniel Zanetti, Bresciani, Katia Denise Saraiva [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.parint.2020.102216
http://hdl.handle.net/11449/208112
Resumo: This research had as objective to evaluate the occurrence and to characterize genetically the infections by Cryptosporidium in Mazama gouazoubira. By a non-invasive harvest methodology using trained sniffer dogs to locate fecal samples of cervids, 642 fecal samples were obtained from six Brazilian localities. The cervids species responsible for the excretion of each fecal sample were identified by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), using the mitochondrial cytochrome b target gene (cyst b) and the restriction enzymes Sspl, AflIII and BstN. From this identification, 437 fecal samples of M. gouazoubira were selected for research of Cryptosporidium spp. performed through negative staining with malachite green and polymerase chain reaction (nPCR), with the subunit of 18S rRNA gene, followed by sequencing the amplified products. In the samples that were diagnosed the presence of parasite species with zoonotic potential, genotyping was also performed using nPCR with the subunit of GP60 gene. Statistical analysis consisted of the Fisher exact test to verify the association of the presence of the enteroparasite in relation to the presence of cattle in each locality, and the McNemar tests and Kappa correlation coefficient used to compare the results obtained between the two diagnostic techniques. In the fecal samples of M. gouazoubira the occurrences of Cryptosporidium were diagnosed in 1.6% (7/437) and 1.1% (5/437), respectively, through nPCR and microscopy. Cryptosporidium. parvum was diagnosed in 100% (7/7) of the samples submitted to sequencing (18S gene). The IIaA16G3R1 subtype was diagnosed in five of the C. parvum samples submitted to genotyping (GP60 gene). This is the first world report of C. parvum in M. gouazoubira and subtype IIaA16G3R1 in cervids.
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spelling Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervidsBrazilCryptosporidium parvumGenotypingMazama gouazoubiraSubtype IIaA16G3R1This research had as objective to evaluate the occurrence and to characterize genetically the infections by Cryptosporidium in Mazama gouazoubira. By a non-invasive harvest methodology using trained sniffer dogs to locate fecal samples of cervids, 642 fecal samples were obtained from six Brazilian localities. The cervids species responsible for the excretion of each fecal sample were identified by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), using the mitochondrial cytochrome b target gene (cyst b) and the restriction enzymes Sspl, AflIII and BstN. From this identification, 437 fecal samples of M. gouazoubira were selected for research of Cryptosporidium spp. performed through negative staining with malachite green and polymerase chain reaction (nPCR), with the subunit of 18S rRNA gene, followed by sequencing the amplified products. In the samples that were diagnosed the presence of parasite species with zoonotic potential, genotyping was also performed using nPCR with the subunit of GP60 gene. Statistical analysis consisted of the Fisher exact test to verify the association of the presence of the enteroparasite in relation to the presence of cattle in each locality, and the McNemar tests and Kappa correlation coefficient used to compare the results obtained between the two diagnostic techniques. In the fecal samples of M. gouazoubira the occurrences of Cryptosporidium were diagnosed in 1.6% (7/437) and 1.1% (5/437), respectively, through nPCR and microscopy. Cryptosporidium. parvum was diagnosed in 100% (7/7) of the samples submitted to sequencing (18S gene). The IIaA16G3R1 subtype was diagnosed in five of the C. parvum samples submitted to genotyping (GP60 gene). This is the first world report of C. parvum in M. gouazoubira and subtype IIaA16G3R1 in cervids.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Federal University of Goiás (UFG), GoiâniaSão Paulo State University (UNESP) School of Veterinary Medicine, Araçatuba. Clóvis Pestana, number 793, CEPSão Paulo State University (UNESP) School of Agricultural and Veterinarian SciencesSão Paulo State University (UNESP) School of Veterinary Medicine, Araçatuba. Clóvis Pestana, number 793, CEPSão Paulo State University (UNESP) School of Agricultural and Veterinarian SciencesFAPESP: 10/50748-3FAPESP: 12/50206-1FAPESP: 15/10086-5FAPESP: 15/25742-5Universidade Federal de Goiás (UFG)Universidade Estadual Paulista (Unesp)Teixeira, Weslen Fabricio Pires [UNESP]De Oliveira, Márcio Leite [UNESP]de Faria Peres, Pedro Henrique [UNESP]Nagata, Walter Bertequini [UNESP]Santana, Bruna Nicoleti [UNESP]Oliveira, Bruno César Miranda [UNESP]Duarte, José Maurício Barbanti [UNESP]Cardoso, Tereza Cristina [UNESP]Lopes, Welber Daniel ZanettiBresciani, Katia Denise Saraiva [UNESP]2021-06-25T11:06:32Z2021-06-25T11:06:32Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.parint.2020.102216Parasitology International, v. 80.1873-03291383-5769http://hdl.handle.net/11449/20811210.1016/j.parint.2020.1022162-s2.0-85095408227Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology Internationalinfo:eu-repo/semantics/openAccess2024-09-04T19:14:50Zoai:repositorio.unesp.br:11449/208112Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:14:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids
title Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids
spellingShingle Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids
Teixeira, Weslen Fabricio Pires [UNESP]
Brazil
Cryptosporidium parvum
Genotyping
Mazama gouazoubira
Subtype IIaA16G3R1
title_short Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids
title_full Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids
title_fullStr Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids
title_full_unstemmed Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids
title_sort Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids
author Teixeira, Weslen Fabricio Pires [UNESP]
author_facet Teixeira, Weslen Fabricio Pires [UNESP]
De Oliveira, Márcio Leite [UNESP]
de Faria Peres, Pedro Henrique [UNESP]
Nagata, Walter Bertequini [UNESP]
Santana, Bruna Nicoleti [UNESP]
Oliveira, Bruno César Miranda [UNESP]
Duarte, José Maurício Barbanti [UNESP]
Cardoso, Tereza Cristina [UNESP]
Lopes, Welber Daniel Zanetti
Bresciani, Katia Denise Saraiva [UNESP]
author_role author
author2 De Oliveira, Márcio Leite [UNESP]
de Faria Peres, Pedro Henrique [UNESP]
Nagata, Walter Bertequini [UNESP]
Santana, Bruna Nicoleti [UNESP]
Oliveira, Bruno César Miranda [UNESP]
Duarte, José Maurício Barbanti [UNESP]
Cardoso, Tereza Cristina [UNESP]
Lopes, Welber Daniel Zanetti
Bresciani, Katia Denise Saraiva [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Goiás (UFG)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Teixeira, Weslen Fabricio Pires [UNESP]
De Oliveira, Márcio Leite [UNESP]
de Faria Peres, Pedro Henrique [UNESP]
Nagata, Walter Bertequini [UNESP]
Santana, Bruna Nicoleti [UNESP]
Oliveira, Bruno César Miranda [UNESP]
Duarte, José Maurício Barbanti [UNESP]
Cardoso, Tereza Cristina [UNESP]
Lopes, Welber Daniel Zanetti
Bresciani, Katia Denise Saraiva [UNESP]
dc.subject.por.fl_str_mv Brazil
Cryptosporidium parvum
Genotyping
Mazama gouazoubira
Subtype IIaA16G3R1
topic Brazil
Cryptosporidium parvum
Genotyping
Mazama gouazoubira
Subtype IIaA16G3R1
description This research had as objective to evaluate the occurrence and to characterize genetically the infections by Cryptosporidium in Mazama gouazoubira. By a non-invasive harvest methodology using trained sniffer dogs to locate fecal samples of cervids, 642 fecal samples were obtained from six Brazilian localities. The cervids species responsible for the excretion of each fecal sample were identified by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), using the mitochondrial cytochrome b target gene (cyst b) and the restriction enzymes Sspl, AflIII and BstN. From this identification, 437 fecal samples of M. gouazoubira were selected for research of Cryptosporidium spp. performed through negative staining with malachite green and polymerase chain reaction (nPCR), with the subunit of 18S rRNA gene, followed by sequencing the amplified products. In the samples that were diagnosed the presence of parasite species with zoonotic potential, genotyping was also performed using nPCR with the subunit of GP60 gene. Statistical analysis consisted of the Fisher exact test to verify the association of the presence of the enteroparasite in relation to the presence of cattle in each locality, and the McNemar tests and Kappa correlation coefficient used to compare the results obtained between the two diagnostic techniques. In the fecal samples of M. gouazoubira the occurrences of Cryptosporidium were diagnosed in 1.6% (7/437) and 1.1% (5/437), respectively, through nPCR and microscopy. Cryptosporidium. parvum was diagnosed in 100% (7/7) of the samples submitted to sequencing (18S gene). The IIaA16G3R1 subtype was diagnosed in five of the C. parvum samples submitted to genotyping (GP60 gene). This is the first world report of C. parvum in M. gouazoubira and subtype IIaA16G3R1 in cervids.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T11:06:32Z
2021-06-25T11:06:32Z
2021-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.parint.2020.102216
Parasitology International, v. 80.
1873-0329
1383-5769
http://hdl.handle.net/11449/208112
10.1016/j.parint.2020.102216
2-s2.0-85095408227
url http://dx.doi.org/10.1016/j.parint.2020.102216
http://hdl.handle.net/11449/208112
identifier_str_mv Parasitology International, v. 80.
1873-0329
1383-5769
10.1016/j.parint.2020.102216
2-s2.0-85095408227
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Parasitology International
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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