Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.parint.2020.102216 http://hdl.handle.net/11449/208112 |
Resumo: | This research had as objective to evaluate the occurrence and to characterize genetically the infections by Cryptosporidium in Mazama gouazoubira. By a non-invasive harvest methodology using trained sniffer dogs to locate fecal samples of cervids, 642 fecal samples were obtained from six Brazilian localities. The cervids species responsible for the excretion of each fecal sample were identified by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), using the mitochondrial cytochrome b target gene (cyst b) and the restriction enzymes Sspl, AflIII and BstN. From this identification, 437 fecal samples of M. gouazoubira were selected for research of Cryptosporidium spp. performed through negative staining with malachite green and polymerase chain reaction (nPCR), with the subunit of 18S rRNA gene, followed by sequencing the amplified products. In the samples that were diagnosed the presence of parasite species with zoonotic potential, genotyping was also performed using nPCR with the subunit of GP60 gene. Statistical analysis consisted of the Fisher exact test to verify the association of the presence of the enteroparasite in relation to the presence of cattle in each locality, and the McNemar tests and Kappa correlation coefficient used to compare the results obtained between the two diagnostic techniques. In the fecal samples of M. gouazoubira the occurrences of Cryptosporidium were diagnosed in 1.6% (7/437) and 1.1% (5/437), respectively, through nPCR and microscopy. Cryptosporidium. parvum was diagnosed in 100% (7/7) of the samples submitted to sequencing (18S gene). The IIaA16G3R1 subtype was diagnosed in five of the C. parvum samples submitted to genotyping (GP60 gene). This is the first world report of C. parvum in M. gouazoubira and subtype IIaA16G3R1 in cervids. |
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Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervidsBrazilCryptosporidium parvumGenotypingMazama gouazoubiraSubtype IIaA16G3R1This research had as objective to evaluate the occurrence and to characterize genetically the infections by Cryptosporidium in Mazama gouazoubira. By a non-invasive harvest methodology using trained sniffer dogs to locate fecal samples of cervids, 642 fecal samples were obtained from six Brazilian localities. The cervids species responsible for the excretion of each fecal sample were identified by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), using the mitochondrial cytochrome b target gene (cyst b) and the restriction enzymes Sspl, AflIII and BstN. From this identification, 437 fecal samples of M. gouazoubira were selected for research of Cryptosporidium spp. performed through negative staining with malachite green and polymerase chain reaction (nPCR), with the subunit of 18S rRNA gene, followed by sequencing the amplified products. In the samples that were diagnosed the presence of parasite species with zoonotic potential, genotyping was also performed using nPCR with the subunit of GP60 gene. Statistical analysis consisted of the Fisher exact test to verify the association of the presence of the enteroparasite in relation to the presence of cattle in each locality, and the McNemar tests and Kappa correlation coefficient used to compare the results obtained between the two diagnostic techniques. In the fecal samples of M. gouazoubira the occurrences of Cryptosporidium were diagnosed in 1.6% (7/437) and 1.1% (5/437), respectively, through nPCR and microscopy. Cryptosporidium. parvum was diagnosed in 100% (7/7) of the samples submitted to sequencing (18S gene). The IIaA16G3R1 subtype was diagnosed in five of the C. parvum samples submitted to genotyping (GP60 gene). This is the first world report of C. parvum in M. gouazoubira and subtype IIaA16G3R1 in cervids.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Federal University of Goiás (UFG), GoiâniaSão Paulo State University (UNESP) School of Veterinary Medicine, Araçatuba. Clóvis Pestana, number 793, CEPSão Paulo State University (UNESP) School of Agricultural and Veterinarian SciencesSão Paulo State University (UNESP) School of Veterinary Medicine, Araçatuba. Clóvis Pestana, number 793, CEPSão Paulo State University (UNESP) School of Agricultural and Veterinarian SciencesFAPESP: 10/50748-3FAPESP: 12/50206-1FAPESP: 15/10086-5FAPESP: 15/25742-5Universidade Federal de Goiás (UFG)Universidade Estadual Paulista (Unesp)Teixeira, Weslen Fabricio Pires [UNESP]De Oliveira, Márcio Leite [UNESP]de Faria Peres, Pedro Henrique [UNESP]Nagata, Walter Bertequini [UNESP]Santana, Bruna Nicoleti [UNESP]Oliveira, Bruno César Miranda [UNESP]Duarte, José Maurício Barbanti [UNESP]Cardoso, Tereza Cristina [UNESP]Lopes, Welber Daniel ZanettiBresciani, Katia Denise Saraiva [UNESP]2021-06-25T11:06:32Z2021-06-25T11:06:32Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.parint.2020.102216Parasitology International, v. 80.1873-03291383-5769http://hdl.handle.net/11449/20811210.1016/j.parint.2020.1022162-s2.0-85095408227Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology Internationalinfo:eu-repo/semantics/openAccess2024-09-04T19:14:50Zoai:repositorio.unesp.br:11449/208112Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:14:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids |
title |
Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids |
spellingShingle |
Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids Teixeira, Weslen Fabricio Pires [UNESP] Brazil Cryptosporidium parvum Genotyping Mazama gouazoubira Subtype IIaA16G3R1 |
title_short |
Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids |
title_full |
Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids |
title_fullStr |
Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids |
title_full_unstemmed |
Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids |
title_sort |
Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids |
author |
Teixeira, Weslen Fabricio Pires [UNESP] |
author_facet |
Teixeira, Weslen Fabricio Pires [UNESP] De Oliveira, Márcio Leite [UNESP] de Faria Peres, Pedro Henrique [UNESP] Nagata, Walter Bertequini [UNESP] Santana, Bruna Nicoleti [UNESP] Oliveira, Bruno César Miranda [UNESP] Duarte, José Maurício Barbanti [UNESP] Cardoso, Tereza Cristina [UNESP] Lopes, Welber Daniel Zanetti Bresciani, Katia Denise Saraiva [UNESP] |
author_role |
author |
author2 |
De Oliveira, Márcio Leite [UNESP] de Faria Peres, Pedro Henrique [UNESP] Nagata, Walter Bertequini [UNESP] Santana, Bruna Nicoleti [UNESP] Oliveira, Bruno César Miranda [UNESP] Duarte, José Maurício Barbanti [UNESP] Cardoso, Tereza Cristina [UNESP] Lopes, Welber Daniel Zanetti Bresciani, Katia Denise Saraiva [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Goiás (UFG) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Teixeira, Weslen Fabricio Pires [UNESP] De Oliveira, Márcio Leite [UNESP] de Faria Peres, Pedro Henrique [UNESP] Nagata, Walter Bertequini [UNESP] Santana, Bruna Nicoleti [UNESP] Oliveira, Bruno César Miranda [UNESP] Duarte, José Maurício Barbanti [UNESP] Cardoso, Tereza Cristina [UNESP] Lopes, Welber Daniel Zanetti Bresciani, Katia Denise Saraiva [UNESP] |
dc.subject.por.fl_str_mv |
Brazil Cryptosporidium parvum Genotyping Mazama gouazoubira Subtype IIaA16G3R1 |
topic |
Brazil Cryptosporidium parvum Genotyping Mazama gouazoubira Subtype IIaA16G3R1 |
description |
This research had as objective to evaluate the occurrence and to characterize genetically the infections by Cryptosporidium in Mazama gouazoubira. By a non-invasive harvest methodology using trained sniffer dogs to locate fecal samples of cervids, 642 fecal samples were obtained from six Brazilian localities. The cervids species responsible for the excretion of each fecal sample were identified by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), using the mitochondrial cytochrome b target gene (cyst b) and the restriction enzymes Sspl, AflIII and BstN. From this identification, 437 fecal samples of M. gouazoubira were selected for research of Cryptosporidium spp. performed through negative staining with malachite green and polymerase chain reaction (nPCR), with the subunit of 18S rRNA gene, followed by sequencing the amplified products. In the samples that were diagnosed the presence of parasite species with zoonotic potential, genotyping was also performed using nPCR with the subunit of GP60 gene. Statistical analysis consisted of the Fisher exact test to verify the association of the presence of the enteroparasite in relation to the presence of cattle in each locality, and the McNemar tests and Kappa correlation coefficient used to compare the results obtained between the two diagnostic techniques. In the fecal samples of M. gouazoubira the occurrences of Cryptosporidium were diagnosed in 1.6% (7/437) and 1.1% (5/437), respectively, through nPCR and microscopy. Cryptosporidium. parvum was diagnosed in 100% (7/7) of the samples submitted to sequencing (18S gene). The IIaA16G3R1 subtype was diagnosed in five of the C. parvum samples submitted to genotyping (GP60 gene). This is the first world report of C. parvum in M. gouazoubira and subtype IIaA16G3R1 in cervids. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:06:32Z 2021-06-25T11:06:32Z 2021-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.parint.2020.102216 Parasitology International, v. 80. 1873-0329 1383-5769 http://hdl.handle.net/11449/208112 10.1016/j.parint.2020.102216 2-s2.0-85095408227 |
url |
http://dx.doi.org/10.1016/j.parint.2020.102216 http://hdl.handle.net/11449/208112 |
identifier_str_mv |
Parasitology International, v. 80. 1873-0329 1383-5769 10.1016/j.parint.2020.102216 2-s2.0-85095408227 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Parasitology International |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021357470089216 |