Comparative transcriptional analyzes of Xanthomonas citri subsp. citri reveal mechanisms of adaptation and bacterial virulence in the early stage of citrus canker disease

Detalhes bibliográficos
Autor(a) principal: Suárez-Acevedo, Sara
Data de Publicação: 2022
Outros Autores: Chaves-Bedoya, Giovanni, Guariz-Pinheiro, Daniel [UNESP], Cristina-Lopes, Aline [UNESP], Mari-Murata, Mayara [UNESP], Hirochi-Herai, Roberto, Aparecido-Ferro, Jesus [UNESP], Rodas-Mendoza, Elkin
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10658-022-02495-w
http://hdl.handle.net/11449/230684
Resumo: Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker, a disease that affects citrus crops of economic importance. Sweet orange cultivars “Pera Rio” (Citrus sinensis) are intermediate susceptible to the disease, while acid lime ‘Galego’ (Citrus aurantifolia) are highly susceptible. To understand the molecular mechanism underlying the differences in Xac response when infecting these two citrus genotypes, in vivo transcriptomic profiles of Xac infecting both genotypes were compared using high throughput sequencing of RNA. Bioinformatics analysis detected 839 and 982 differentially expressed genes (DEGs) in Xac during their interaction with sweet orange and acid lime compared to controls, respectively. Moreover, 607 DEG genes were found to be common in both genotypes. In both hosts, the annotation of DEGs showed that Xac induced genes involved with the Type III Secretion System and corresponding effector genes, cell wall degrading enzymes, and xanthan gum synthesis. In addition, higher expression values in Xac genes associated with oxidative stress resistance were found in their interaction with sweet orange, compared to acid lime. The results collectively allow the proposition of the main genetic modulation mechanism used by Xac to develop the disease in the early stages of host infection and reveal different transcriptional responses when the bacteria interact with C. sinensis and C. aurantifolia. Thus, our study provides information about the intercellular environment of Xac and the possible strategies it uses to promote the disease.
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spelling Comparative transcriptional analyzes of Xanthomonas citri subsp. citri reveal mechanisms of adaptation and bacterial virulence in the early stage of citrus canker diseaseDifferentially expressed genesRNA-SeqSweet orangeXanthomonas citriXanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker, a disease that affects citrus crops of economic importance. Sweet orange cultivars “Pera Rio” (Citrus sinensis) are intermediate susceptible to the disease, while acid lime ‘Galego’ (Citrus aurantifolia) are highly susceptible. To understand the molecular mechanism underlying the differences in Xac response when infecting these two citrus genotypes, in vivo transcriptomic profiles of Xac infecting both genotypes were compared using high throughput sequencing of RNA. Bioinformatics analysis detected 839 and 982 differentially expressed genes (DEGs) in Xac during their interaction with sweet orange and acid lime compared to controls, respectively. Moreover, 607 DEG genes were found to be common in both genotypes. In both hosts, the annotation of DEGs showed that Xac induced genes involved with the Type III Secretion System and corresponding effector genes, cell wall degrading enzymes, and xanthan gum synthesis. In addition, higher expression values in Xac genes associated with oxidative stress resistance were found in their interaction with sweet orange, compared to acid lime. The results collectively allow the proposition of the main genetic modulation mechanism used by Xac to develop the disease in the early stages of host infection and reveal different transcriptional responses when the bacteria interact with C. sinensis and C. aurantifolia. Thus, our study provides information about the intercellular environment of Xac and the possible strategies it uses to promote the disease.FITOBIOMOL Research Group School of Basic Sciences Francisco de Paula Santander University, Avenida Gran Colombia No 12E96, Barrio ColsagDepartment of Technology School of Agricultural and Veterinary Sciences São Paulo State University-UNESP, JaboticabalExperimental Multiuser Laboratory (LEM) Graduate Program in Health Sciences (PPGCS) School of Medicine Pontifícia Universidade Católica do Paraná, CuritibaDepartment of Technology School of Agricultural and Veterinary Sciences São Paulo State University-UNESP, JaboticabalFrancisco de Paula Santander UniversityUniversidade Estadual Paulista (UNESP)Pontifícia Universidade Católica do ParanáSuárez-Acevedo, SaraChaves-Bedoya, GiovanniGuariz-Pinheiro, Daniel [UNESP]Cristina-Lopes, Aline [UNESP]Mari-Murata, Mayara [UNESP]Hirochi-Herai, RobertoAparecido-Ferro, Jesus [UNESP]Rodas-Mendoza, Elkin2022-04-29T08:41:30Z2022-04-29T08:41:30Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10658-022-02495-wEuropean Journal of Plant Pathology.1573-84690929-1873http://hdl.handle.net/11449/23068410.1007/s10658-022-02495-w2-s2.0-85127691489Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEuropean Journal of Plant Pathologyinfo:eu-repo/semantics/openAccess2024-06-07T15:31:09Zoai:repositorio.unesp.br:11449/230684Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:41:28.788292Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Comparative transcriptional analyzes of Xanthomonas citri subsp. citri reveal mechanisms of adaptation and bacterial virulence in the early stage of citrus canker disease
title Comparative transcriptional analyzes of Xanthomonas citri subsp. citri reveal mechanisms of adaptation and bacterial virulence in the early stage of citrus canker disease
spellingShingle Comparative transcriptional analyzes of Xanthomonas citri subsp. citri reveal mechanisms of adaptation and bacterial virulence in the early stage of citrus canker disease
Suárez-Acevedo, Sara
Differentially expressed genes
RNA-Seq
Sweet orange
Xanthomonas citri
title_short Comparative transcriptional analyzes of Xanthomonas citri subsp. citri reveal mechanisms of adaptation and bacterial virulence in the early stage of citrus canker disease
title_full Comparative transcriptional analyzes of Xanthomonas citri subsp. citri reveal mechanisms of adaptation and bacterial virulence in the early stage of citrus canker disease
title_fullStr Comparative transcriptional analyzes of Xanthomonas citri subsp. citri reveal mechanisms of adaptation and bacterial virulence in the early stage of citrus canker disease
title_full_unstemmed Comparative transcriptional analyzes of Xanthomonas citri subsp. citri reveal mechanisms of adaptation and bacterial virulence in the early stage of citrus canker disease
title_sort Comparative transcriptional analyzes of Xanthomonas citri subsp. citri reveal mechanisms of adaptation and bacterial virulence in the early stage of citrus canker disease
author Suárez-Acevedo, Sara
author_facet Suárez-Acevedo, Sara
Chaves-Bedoya, Giovanni
Guariz-Pinheiro, Daniel [UNESP]
Cristina-Lopes, Aline [UNESP]
Mari-Murata, Mayara [UNESP]
Hirochi-Herai, Roberto
Aparecido-Ferro, Jesus [UNESP]
Rodas-Mendoza, Elkin
author_role author
author2 Chaves-Bedoya, Giovanni
Guariz-Pinheiro, Daniel [UNESP]
Cristina-Lopes, Aline [UNESP]
Mari-Murata, Mayara [UNESP]
Hirochi-Herai, Roberto
Aparecido-Ferro, Jesus [UNESP]
Rodas-Mendoza, Elkin
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Francisco de Paula Santander University
Universidade Estadual Paulista (UNESP)
Pontifícia Universidade Católica do Paraná
dc.contributor.author.fl_str_mv Suárez-Acevedo, Sara
Chaves-Bedoya, Giovanni
Guariz-Pinheiro, Daniel [UNESP]
Cristina-Lopes, Aline [UNESP]
Mari-Murata, Mayara [UNESP]
Hirochi-Herai, Roberto
Aparecido-Ferro, Jesus [UNESP]
Rodas-Mendoza, Elkin
dc.subject.por.fl_str_mv Differentially expressed genes
RNA-Seq
Sweet orange
Xanthomonas citri
topic Differentially expressed genes
RNA-Seq
Sweet orange
Xanthomonas citri
description Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker, a disease that affects citrus crops of economic importance. Sweet orange cultivars “Pera Rio” (Citrus sinensis) are intermediate susceptible to the disease, while acid lime ‘Galego’ (Citrus aurantifolia) are highly susceptible. To understand the molecular mechanism underlying the differences in Xac response when infecting these two citrus genotypes, in vivo transcriptomic profiles of Xac infecting both genotypes were compared using high throughput sequencing of RNA. Bioinformatics analysis detected 839 and 982 differentially expressed genes (DEGs) in Xac during their interaction with sweet orange and acid lime compared to controls, respectively. Moreover, 607 DEG genes were found to be common in both genotypes. In both hosts, the annotation of DEGs showed that Xac induced genes involved with the Type III Secretion System and corresponding effector genes, cell wall degrading enzymes, and xanthan gum synthesis. In addition, higher expression values in Xac genes associated with oxidative stress resistance were found in their interaction with sweet orange, compared to acid lime. The results collectively allow the proposition of the main genetic modulation mechanism used by Xac to develop the disease in the early stages of host infection and reveal different transcriptional responses when the bacteria interact with C. sinensis and C. aurantifolia. Thus, our study provides information about the intercellular environment of Xac and the possible strategies it uses to promote the disease.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:41:30Z
2022-04-29T08:41:30Z
2022-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10658-022-02495-w
European Journal of Plant Pathology.
1573-8469
0929-1873
http://hdl.handle.net/11449/230684
10.1007/s10658-022-02495-w
2-s2.0-85127691489
url http://dx.doi.org/10.1007/s10658-022-02495-w
http://hdl.handle.net/11449/230684
identifier_str_mv European Journal of Plant Pathology.
1573-8469
0929-1873
10.1007/s10658-022-02495-w
2-s2.0-85127691489
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv European Journal of Plant Pathology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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