Cytogenetic and molecular data demonstrate that the bryconinae (ostariophysi, bryconidae) species from southeastern Brazil form a phylogenetic and phylogeographic unit

Detalhes bibliográficos
Autor(a) principal: Travenzoli, Natália Martins
Data de Publicação: 2015
Outros Autores: Silva, Priscilla Caroline, Santos, Udson, Zanuncio, José Cola, Oliveira, Claudio [UNESP], Dergam, Jorge Abdala
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0137843
http://hdl.handle.net/11449/131238
Resumo: Brycon spp. occur in Neotropical watersheds to the west and east of the Andes, and as they are sensitive to anthropogenic changes, many these species are endangered in southeastern Brazil. Coastal rivers in southeastern Brazil are characterized by the presence of relatively few freshwater fish species and high endemism of this fauna. The objective of this study was to examine whether Brycon spp. occurring in the coastal basins of southeastern Brazil are monophyletic, using cytogenetic data, mitochondrial, and nuclear molecular markers. All the species showed a diploid number of 50 chromosomes, a conserved number within the subfamily Bryconinae. However, the karyotypic formulas were unique to most species, including Brycon devillei (26m+22sm+2st), Brycon ferox (26m+12sm+12st), Brycon insignis (22m+20sm+8st), Brycon opalinus, and Brycon vermelha (24m+20sm+6st), indicating the prevalence of pericentric and paracentric inversions in the chromosomal evolution of these species. All of them had nucleolar organizer regions in the first pair of subtelocentric chromosomes and no equilocal distribution of heterochromatin in the first pair of chromosomes of the karyotype. These two features, not seen in any other Brycon spp. examined to date, indicate that Bryconinae species from the Brazilian southeastern coastal basins, including the monotypic genus Henochilus, are monophyletic. Also, this is the first study that reports NOR location and C-banding patterns as synapomorphies for a Neotropical fish species group. The monophyly was also supported by a phylogenetic analysis of 16S rDNA (16S), cytochrome oxidase subunit I (COI), alpha-myosin (MYH6) genes and S72 intron molecular data. Our results partially corroborate the Brycon acuminatus group proposed by Howes in 1982: our proposed clade keeps B. devillei, B. ferox, and B. insignis; but it also includes B. opalinus, B. vermelha, and H. weatlandii whereas it excludes B. nattereri. The phylogeographic unit formed by Bryconinae species in southeastern Brazil reflects the long and isolated paleohydrological history of these coastal basins relative to the continental watersheds.
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spelling Cytogenetic and molecular data demonstrate that the bryconinae (ostariophysi, bryconidae) species from southeastern Brazil form a phylogenetic and phylogeographic unitBrycon spp. occur in Neotropical watersheds to the west and east of the Andes, and as they are sensitive to anthropogenic changes, many these species are endangered in southeastern Brazil. Coastal rivers in southeastern Brazil are characterized by the presence of relatively few freshwater fish species and high endemism of this fauna. The objective of this study was to examine whether Brycon spp. occurring in the coastal basins of southeastern Brazil are monophyletic, using cytogenetic data, mitochondrial, and nuclear molecular markers. All the species showed a diploid number of 50 chromosomes, a conserved number within the subfamily Bryconinae. However, the karyotypic formulas were unique to most species, including Brycon devillei (26m+22sm+2st), Brycon ferox (26m+12sm+12st), Brycon insignis (22m+20sm+8st), Brycon opalinus, and Brycon vermelha (24m+20sm+6st), indicating the prevalence of pericentric and paracentric inversions in the chromosomal evolution of these species. All of them had nucleolar organizer regions in the first pair of subtelocentric chromosomes and no equilocal distribution of heterochromatin in the first pair of chromosomes of the karyotype. These two features, not seen in any other Brycon spp. examined to date, indicate that Bryconinae species from the Brazilian southeastern coastal basins, including the monotypic genus Henochilus, are monophyletic. Also, this is the first study that reports NOR location and C-banding patterns as synapomorphies for a Neotropical fish species group. The monophyly was also supported by a phylogenetic analysis of 16S rDNA (16S), cytochrome oxidase subunit I (COI), alpha-myosin (MYH6) genes and S72 intron molecular data. Our results partially corroborate the Brycon acuminatus group proposed by Howes in 1982: our proposed clade keeps B. devillei, B. ferox, and B. insignis; but it also includes B. opalinus, B. vermelha, and H. weatlandii whereas it excludes B. nattereri. The phylogeographic unit formed by Bryconinae species in southeastern Brazil reflects the long and isolated paleohydrological history of these coastal basins relative to the continental watersheds.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Laboratório de Sistemática Molecular-Beagle, Departamento de Biologia Animal, Universidade Federal de Viçosa, CEP 36570-000, Viçosa, Minas Gerais, Brazil.Instituto de Biociências, Departamento de Morfologia, Universidade Estadual Paulista (UNESP), CEP 18618-970, Botucatu, São Paulo, Brazil.Instituto de Biociências, Departamento de Morfologia, Universidade Estadual Paulista (UNESP), CEP 18618-970, Botucatu, São Paulo, Brazil.Public Library ScienceUniversidade Federal de Viçosa (UFV)Universidade Estadual Paulista (Unesp)Travenzoli, Natália MartinsSilva, Priscilla CarolineSantos, UdsonZanuncio, José ColaOliveira, Claudio [UNESP]Dergam, Jorge Abdala2015-12-07T15:32:56Z2015-12-07T15:32:56Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0137843Plos One, v. 10, n. 9, 2015.1932-6203http://hdl.handle.net/11449/13123810.1371/journal.pone.0137843PMC4570709.pdf26372558PMC4570709PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One2.7661,164info:eu-repo/semantics/openAccess2023-12-05T06:20:17Zoai:repositorio.unesp.br:11449/131238Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:34:11.020756Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cytogenetic and molecular data demonstrate that the bryconinae (ostariophysi, bryconidae) species from southeastern Brazil form a phylogenetic and phylogeographic unit
title Cytogenetic and molecular data demonstrate that the bryconinae (ostariophysi, bryconidae) species from southeastern Brazil form a phylogenetic and phylogeographic unit
spellingShingle Cytogenetic and molecular data demonstrate that the bryconinae (ostariophysi, bryconidae) species from southeastern Brazil form a phylogenetic and phylogeographic unit
Travenzoli, Natália Martins
title_short Cytogenetic and molecular data demonstrate that the bryconinae (ostariophysi, bryconidae) species from southeastern Brazil form a phylogenetic and phylogeographic unit
title_full Cytogenetic and molecular data demonstrate that the bryconinae (ostariophysi, bryconidae) species from southeastern Brazil form a phylogenetic and phylogeographic unit
title_fullStr Cytogenetic and molecular data demonstrate that the bryconinae (ostariophysi, bryconidae) species from southeastern Brazil form a phylogenetic and phylogeographic unit
title_full_unstemmed Cytogenetic and molecular data demonstrate that the bryconinae (ostariophysi, bryconidae) species from southeastern Brazil form a phylogenetic and phylogeographic unit
title_sort Cytogenetic and molecular data demonstrate that the bryconinae (ostariophysi, bryconidae) species from southeastern Brazil form a phylogenetic and phylogeographic unit
author Travenzoli, Natália Martins
author_facet Travenzoli, Natália Martins
Silva, Priscilla Caroline
Santos, Udson
Zanuncio, José Cola
Oliveira, Claudio [UNESP]
Dergam, Jorge Abdala
author_role author
author2 Silva, Priscilla Caroline
Santos, Udson
Zanuncio, José Cola
Oliveira, Claudio [UNESP]
Dergam, Jorge Abdala
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Viçosa (UFV)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Travenzoli, Natália Martins
Silva, Priscilla Caroline
Santos, Udson
Zanuncio, José Cola
Oliveira, Claudio [UNESP]
Dergam, Jorge Abdala
description Brycon spp. occur in Neotropical watersheds to the west and east of the Andes, and as they are sensitive to anthropogenic changes, many these species are endangered in southeastern Brazil. Coastal rivers in southeastern Brazil are characterized by the presence of relatively few freshwater fish species and high endemism of this fauna. The objective of this study was to examine whether Brycon spp. occurring in the coastal basins of southeastern Brazil are monophyletic, using cytogenetic data, mitochondrial, and nuclear molecular markers. All the species showed a diploid number of 50 chromosomes, a conserved number within the subfamily Bryconinae. However, the karyotypic formulas were unique to most species, including Brycon devillei (26m+22sm+2st), Brycon ferox (26m+12sm+12st), Brycon insignis (22m+20sm+8st), Brycon opalinus, and Brycon vermelha (24m+20sm+6st), indicating the prevalence of pericentric and paracentric inversions in the chromosomal evolution of these species. All of them had nucleolar organizer regions in the first pair of subtelocentric chromosomes and no equilocal distribution of heterochromatin in the first pair of chromosomes of the karyotype. These two features, not seen in any other Brycon spp. examined to date, indicate that Bryconinae species from the Brazilian southeastern coastal basins, including the monotypic genus Henochilus, are monophyletic. Also, this is the first study that reports NOR location and C-banding patterns as synapomorphies for a Neotropical fish species group. The monophyly was also supported by a phylogenetic analysis of 16S rDNA (16S), cytochrome oxidase subunit I (COI), alpha-myosin (MYH6) genes and S72 intron molecular data. Our results partially corroborate the Brycon acuminatus group proposed by Howes in 1982: our proposed clade keeps B. devillei, B. ferox, and B. insignis; but it also includes B. opalinus, B. vermelha, and H. weatlandii whereas it excludes B. nattereri. The phylogeographic unit formed by Bryconinae species in southeastern Brazil reflects the long and isolated paleohydrological history of these coastal basins relative to the continental watersheds.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-07T15:32:56Z
2015-12-07T15:32:56Z
2015
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dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0137843
Plos One, v. 10, n. 9, 2015.
1932-6203
http://hdl.handle.net/11449/131238
10.1371/journal.pone.0137843
PMC4570709.pdf
26372558
PMC4570709
url http://dx.doi.org/10.1371/journal.pone.0137843
http://hdl.handle.net/11449/131238
identifier_str_mv Plos One, v. 10, n. 9, 2015.
1932-6203
10.1371/journal.pone.0137843
PMC4570709.pdf
26372558
PMC4570709
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publisher.none.fl_str_mv Public Library Science
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