Development, characterization and clinical use of a biodegradable composite scaffold for bone engineering in oro-maxillo-facial surgery
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4161/org.6.3.12392 http://hdl.handle.net/11449/42566 |
Resumo: | We have developed a biodegradable composite scaffold for bone tissue engineering applications with a pore size and interconnecting macroporosity similar to those of human trabecular bone. The scaffold is fabricated by a process of particle leaching and phase inversion from poly(lactide-co-glycolide) (PLGA) and two calcium phosphate (CaP) phases both of which are resorbable by osteoclasts; the first a particulate within the polymer structure and the second a thin ubiquitous coating. The 3-5 mu m thick osteoconductive surface CaP abrogates the putative foreign body giant cell response to the underlying polymer, while the internal CaP phase provides dimensional stability in an otherwise highly compliant structure. The scaffold may be used as a biomaterial alone, as a carrier for cells or a three-phase drug delivery device. Due to the highly interconnected macroporosity ranging from 81% to 91%, with macropores of 0.8 similar to 1.8 mm, and an ability to wick up blood, the scaffold acts as both a clot-retention device and an osteoconductive support for host bone growth. As a cell delivery vehicle, the scaffold can be first seeded with human mesenchymal cells which can then contribute to bone formation in orthotopic implantation sites, as we show in immune-compromised animal hosts. We have also employed this scaffold in both lithomorph and particulate forms in human patients to maintain alveolar bone height following tooth extraction, and augment alveolar bone height through standard sinus lift approaches. We provide a clinical case report of both of these applications; and we show that the scaffold served to regenerate sufficient bone tissue in the wound site to provide a sound foundation for dental implant placement. At the time of writing, such implants have been in occlusal function for periods of up to 3 years in sites regenerated through the use of the scaffold. |
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Development, characterization and clinical use of a biodegradable composite scaffold for bone engineering in oro-maxillo-facial surgerybone regenerationscaffoldcompositebiodegradableclot retentionosteoconductioncell deliveryextraction socketsinus liftclinicalWe have developed a biodegradable composite scaffold for bone tissue engineering applications with a pore size and interconnecting macroporosity similar to those of human trabecular bone. The scaffold is fabricated by a process of particle leaching and phase inversion from poly(lactide-co-glycolide) (PLGA) and two calcium phosphate (CaP) phases both of which are resorbable by osteoclasts; the first a particulate within the polymer structure and the second a thin ubiquitous coating. The 3-5 mu m thick osteoconductive surface CaP abrogates the putative foreign body giant cell response to the underlying polymer, while the internal CaP phase provides dimensional stability in an otherwise highly compliant structure. The scaffold may be used as a biomaterial alone, as a carrier for cells or a three-phase drug delivery device. Due to the highly interconnected macroporosity ranging from 81% to 91%, with macropores of 0.8 similar to 1.8 mm, and an ability to wick up blood, the scaffold acts as both a clot-retention device and an osteoconductive support for host bone growth. As a cell delivery vehicle, the scaffold can be first seeded with human mesenchymal cells which can then contribute to bone formation in orthotopic implantation sites, as we show in immune-compromised animal hosts. We have also employed this scaffold in both lithomorph and particulate forms in human patients to maintain alveolar bone height following tooth extraction, and augment alveolar bone height through standard sinus lift approaches. We provide a clinical case report of both of these applications; and we show that the scaffold served to regenerate sufficient bone tissue in the wound site to provide a sound foundation for dental implant placement. At the time of writing, such implants have been in occlusal function for periods of up to 3 years in sites regenerated through the use of the scaffold.BoneTec CorporationOntario Research and Development Challenge Fund (ORDCF)Canadian Institutes of Health Research (CIHR)Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON, CanadaUniv Toronto, Fac Dent, Toronto, ON, CanadaUniv Estadual Paulista, Fac Dent, São Paulo, BrazilUniv São Paulo, São Paulo, BrazilUniv Estadual Paulista, Fac Dent, São Paulo, BrazilLandes BioscienceUniv TorontoUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Davies, John E.Matta, RanoMendes, Vanessa C.Perri de Carvalho, Paulo S. [UNESP]2014-05-20T15:34:32Z2014-05-20T15:34:32Z2010-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article161-166application/pdfhttp://dx.doi.org/10.4161/org.6.3.12392Organogenesis. Austin: Landes Bioscience, v. 6, n. 3, p. 161-166, 2010.1547-6278http://hdl.handle.net/11449/4256610.4161/org.6.3.12392WOS:000290266200005WOS000290266200005.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOrganogenesis2.5671,320info:eu-repo/semantics/openAccess2023-11-19T06:09:53Zoai:repositorio.unesp.br:11449/42566Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:07:05.728840Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development, characterization and clinical use of a biodegradable composite scaffold for bone engineering in oro-maxillo-facial surgery |
title |
Development, characterization and clinical use of a biodegradable composite scaffold for bone engineering in oro-maxillo-facial surgery |
spellingShingle |
Development, characterization and clinical use of a biodegradable composite scaffold for bone engineering in oro-maxillo-facial surgery Davies, John E. bone regeneration scaffold composite biodegradable clot retention osteoconduction cell delivery extraction socket sinus lift clinical |
title_short |
Development, characterization and clinical use of a biodegradable composite scaffold for bone engineering in oro-maxillo-facial surgery |
title_full |
Development, characterization and clinical use of a biodegradable composite scaffold for bone engineering in oro-maxillo-facial surgery |
title_fullStr |
Development, characterization and clinical use of a biodegradable composite scaffold for bone engineering in oro-maxillo-facial surgery |
title_full_unstemmed |
Development, characterization and clinical use of a biodegradable composite scaffold for bone engineering in oro-maxillo-facial surgery |
title_sort |
Development, characterization and clinical use of a biodegradable composite scaffold for bone engineering in oro-maxillo-facial surgery |
author |
Davies, John E. |
author_facet |
Davies, John E. Matta, Rano Mendes, Vanessa C. Perri de Carvalho, Paulo S. [UNESP] |
author_role |
author |
author2 |
Matta, Rano Mendes, Vanessa C. Perri de Carvalho, Paulo S. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Univ Toronto Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Davies, John E. Matta, Rano Mendes, Vanessa C. Perri de Carvalho, Paulo S. [UNESP] |
dc.subject.por.fl_str_mv |
bone regeneration scaffold composite biodegradable clot retention osteoconduction cell delivery extraction socket sinus lift clinical |
topic |
bone regeneration scaffold composite biodegradable clot retention osteoconduction cell delivery extraction socket sinus lift clinical |
description |
We have developed a biodegradable composite scaffold for bone tissue engineering applications with a pore size and interconnecting macroporosity similar to those of human trabecular bone. The scaffold is fabricated by a process of particle leaching and phase inversion from poly(lactide-co-glycolide) (PLGA) and two calcium phosphate (CaP) phases both of which are resorbable by osteoclasts; the first a particulate within the polymer structure and the second a thin ubiquitous coating. The 3-5 mu m thick osteoconductive surface CaP abrogates the putative foreign body giant cell response to the underlying polymer, while the internal CaP phase provides dimensional stability in an otherwise highly compliant structure. The scaffold may be used as a biomaterial alone, as a carrier for cells or a three-phase drug delivery device. Due to the highly interconnected macroporosity ranging from 81% to 91%, with macropores of 0.8 similar to 1.8 mm, and an ability to wick up blood, the scaffold acts as both a clot-retention device and an osteoconductive support for host bone growth. As a cell delivery vehicle, the scaffold can be first seeded with human mesenchymal cells which can then contribute to bone formation in orthotopic implantation sites, as we show in immune-compromised animal hosts. We have also employed this scaffold in both lithomorph and particulate forms in human patients to maintain alveolar bone height following tooth extraction, and augment alveolar bone height through standard sinus lift approaches. We provide a clinical case report of both of these applications; and we show that the scaffold served to regenerate sufficient bone tissue in the wound site to provide a sound foundation for dental implant placement. At the time of writing, such implants have been in occlusal function for periods of up to 3 years in sites regenerated through the use of the scaffold. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-07-01 2014-05-20T15:34:32Z 2014-05-20T15:34:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4161/org.6.3.12392 Organogenesis. Austin: Landes Bioscience, v. 6, n. 3, p. 161-166, 2010. 1547-6278 http://hdl.handle.net/11449/42566 10.4161/org.6.3.12392 WOS:000290266200005 WOS000290266200005.pdf |
url |
http://dx.doi.org/10.4161/org.6.3.12392 http://hdl.handle.net/11449/42566 |
identifier_str_mv |
Organogenesis. Austin: Landes Bioscience, v. 6, n. 3, p. 161-166, 2010. 1547-6278 10.4161/org.6.3.12392 WOS:000290266200005 WOS000290266200005.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Organogenesis 2.567 1,320 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
161-166 application/pdf |
dc.publisher.none.fl_str_mv |
Landes Bioscience |
publisher.none.fl_str_mv |
Landes Bioscience |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128897426915328 |