Candida krusei and Candida glabrata reduce the filamentation of Candida albicans by downregulating expression of HWP1 gene
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s12223-017-0500-4 http://hdl.handle.net/11449/178617 |
Resumo: | Pathogenicity of Candida albicans is associated with its capacity switch from yeast-like to hyphal growth. The hyphal form is capable to penetrate the epithelial surfaces and to damage the host tissues. Therefore, many investigations have focused on mechanisms that control the morphological transitions of C. albicans. Recently, certain studies have showed that non-albicans Candida species can reduce the capacity of C. albicans to form biofilms and to develop candidiasis in animal models. Then, the objective of this study was to evaluate the effects of Candida krusei and Candida glabrata on the morphogenesis of C. albicans. Firstly, the capacity of reference and clinical strains of C. albicans in forming hyphae was tested in vitro. After that, the expression of HWP1 (hyphal wall protein 1) gene was determined by quantitative real-time PCR (polymerase chain reaction) assay. For both reference and clinical strains, a significant inhibition of the hyphae formation was observed when C. albicans was incubated in the presence of C. krusei or C. glabrata compared to the control group composed only by C. albicans. In addition, the culture mixed of C. albicans-C. krusei or C. albicans-C. glabrata reduced significantly the expression of HWP1 gene of C. albicans in relation to single cultures of this specie. In both filamentation and gene expression assays, C. krusei showed the higher inhibitory activity on the morphogenesis of C. albicans compared to C. glabrata. C. krusei and C. glabrata are capable to reduce the filamentation of C. albicans and consequently decrease the expression of the HWP1 gene. |
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spelling |
Candida krusei and Candida glabrata reduce the filamentation of Candida albicans by downregulating expression of HWP1 genePathogenicity of Candida albicans is associated with its capacity switch from yeast-like to hyphal growth. The hyphal form is capable to penetrate the epithelial surfaces and to damage the host tissues. Therefore, many investigations have focused on mechanisms that control the morphological transitions of C. albicans. Recently, certain studies have showed that non-albicans Candida species can reduce the capacity of C. albicans to form biofilms and to develop candidiasis in animal models. Then, the objective of this study was to evaluate the effects of Candida krusei and Candida glabrata on the morphogenesis of C. albicans. Firstly, the capacity of reference and clinical strains of C. albicans in forming hyphae was tested in vitro. After that, the expression of HWP1 (hyphal wall protein 1) gene was determined by quantitative real-time PCR (polymerase chain reaction) assay. For both reference and clinical strains, a significant inhibition of the hyphae formation was observed when C. albicans was incubated in the presence of C. krusei or C. glabrata compared to the control group composed only by C. albicans. In addition, the culture mixed of C. albicans-C. krusei or C. albicans-C. glabrata reduced significantly the expression of HWP1 gene of C. albicans in relation to single cultures of this specie. In both filamentation and gene expression assays, C. krusei showed the higher inhibitory activity on the morphogenesis of C. albicans compared to C. glabrata. C. krusei and C. glabrata are capable to reduce the filamentation of C. albicans and consequently decrease the expression of the HWP1 gene.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biosciences and Oral Diagnosis Institute of Science and Technology Univ Estadual Paulista – UNESP, Francisco José Longo 777, São DimasDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology Univ Estadual Paulista – UNESP, Francisco José Longo 777, São DimasFAPESP: 2011/15194-0Universidade Estadual Paulista (Unesp)de Barros, Patrícia Pimentel [UNESP]Freire, Fernanda [UNESP]Rossoni, Rodnei Dennis [UNESP]Junqueira, Juliana Campos [UNESP]Jorge, Antonio Olavo Cardoso [UNESP]2018-12-11T17:31:20Z2018-12-11T17:31:20Z2017-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article317-323application/pdfhttp://dx.doi.org/10.1007/s12223-017-0500-4Folia Microbiologica, v. 62, n. 4, p. 317-323, 2017.1874-93560015-5632http://hdl.handle.net/11449/17861710.1007/s12223-017-0500-42-s2.0-850115795632-s2.0-85011579563.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFolia Microbiologica0,502info:eu-repo/semantics/openAccess2023-12-10T06:19:57Zoai:repositorio.unesp.br:11449/178617Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:56:55.677929Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Candida krusei and Candida glabrata reduce the filamentation of Candida albicans by downregulating expression of HWP1 gene |
title |
Candida krusei and Candida glabrata reduce the filamentation of Candida albicans by downregulating expression of HWP1 gene |
spellingShingle |
Candida krusei and Candida glabrata reduce the filamentation of Candida albicans by downregulating expression of HWP1 gene de Barros, Patrícia Pimentel [UNESP] |
title_short |
Candida krusei and Candida glabrata reduce the filamentation of Candida albicans by downregulating expression of HWP1 gene |
title_full |
Candida krusei and Candida glabrata reduce the filamentation of Candida albicans by downregulating expression of HWP1 gene |
title_fullStr |
Candida krusei and Candida glabrata reduce the filamentation of Candida albicans by downregulating expression of HWP1 gene |
title_full_unstemmed |
Candida krusei and Candida glabrata reduce the filamentation of Candida albicans by downregulating expression of HWP1 gene |
title_sort |
Candida krusei and Candida glabrata reduce the filamentation of Candida albicans by downregulating expression of HWP1 gene |
author |
de Barros, Patrícia Pimentel [UNESP] |
author_facet |
de Barros, Patrícia Pimentel [UNESP] Freire, Fernanda [UNESP] Rossoni, Rodnei Dennis [UNESP] Junqueira, Juliana Campos [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] |
author_role |
author |
author2 |
Freire, Fernanda [UNESP] Rossoni, Rodnei Dennis [UNESP] Junqueira, Juliana Campos [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de Barros, Patrícia Pimentel [UNESP] Freire, Fernanda [UNESP] Rossoni, Rodnei Dennis [UNESP] Junqueira, Juliana Campos [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] |
description |
Pathogenicity of Candida albicans is associated with its capacity switch from yeast-like to hyphal growth. The hyphal form is capable to penetrate the epithelial surfaces and to damage the host tissues. Therefore, many investigations have focused on mechanisms that control the morphological transitions of C. albicans. Recently, certain studies have showed that non-albicans Candida species can reduce the capacity of C. albicans to form biofilms and to develop candidiasis in animal models. Then, the objective of this study was to evaluate the effects of Candida krusei and Candida glabrata on the morphogenesis of C. albicans. Firstly, the capacity of reference and clinical strains of C. albicans in forming hyphae was tested in vitro. After that, the expression of HWP1 (hyphal wall protein 1) gene was determined by quantitative real-time PCR (polymerase chain reaction) assay. For both reference and clinical strains, a significant inhibition of the hyphae formation was observed when C. albicans was incubated in the presence of C. krusei or C. glabrata compared to the control group composed only by C. albicans. In addition, the culture mixed of C. albicans-C. krusei or C. albicans-C. glabrata reduced significantly the expression of HWP1 gene of C. albicans in relation to single cultures of this specie. In both filamentation and gene expression assays, C. krusei showed the higher inhibitory activity on the morphogenesis of C. albicans compared to C. glabrata. C. krusei and C. glabrata are capable to reduce the filamentation of C. albicans and consequently decrease the expression of the HWP1 gene. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-01 2018-12-11T17:31:20Z 2018-12-11T17:31:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12223-017-0500-4 Folia Microbiologica, v. 62, n. 4, p. 317-323, 2017. 1874-9356 0015-5632 http://hdl.handle.net/11449/178617 10.1007/s12223-017-0500-4 2-s2.0-85011579563 2-s2.0-85011579563.pdf |
url |
http://dx.doi.org/10.1007/s12223-017-0500-4 http://hdl.handle.net/11449/178617 |
identifier_str_mv |
Folia Microbiologica, v. 62, n. 4, p. 317-323, 2017. 1874-9356 0015-5632 10.1007/s12223-017-0500-4 2-s2.0-85011579563 2-s2.0-85011579563.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Folia Microbiologica 0,502 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
317-323 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129142474932224 |