Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors

Detalhes bibliográficos
Autor(a) principal: Martinez, Paula F. [UNESP]
Data de Publicação: 2010
Outros Autores: Okoshi, Katashi [UNESP], Zornoff, Leonardo Antonio Mamede [UNESP], Carvalho, Robson F. [UNESP], Oliveira Junior, Silvio A. [UNESP], Lima, Aline R. R. [UNESP], Campos, Dijon H. S. [UNESP], Damatto, Ricardo L. [UNESP], Padovani, Carlos Roberto [UNESP], Nogueira, Célia Regina [UNESP], Pai-Silva, Maeli Dai [UNESP], Okoshi, Marina Politi [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.medscimonit.com/abstract/index/idArt/881289
http://hdl.handle.net/11449/17086
Resumo: Background: Although intrinsic skeletal muscle abnormalities can influence exercise intolerance during heart failure (HF), the factors responsible for muscle changes have not been elucidated. In this study we evaluated the expression of myogenic regulatory factors (MRF), myosin heavy chain (MyHC) isoforms, and fiber trophism in the soleus muscle of rats with myocardial infarction-induced heart failure.Method/Results: Six months after surgery, 2 groups of rats were studied: sham, and infarcted rats with HF (MI/HF+, MI size: 41.1 +/- 6.3% of total left ventricular area). In the infarcted group, microscopic evaluation revealed scattered foci of fiber necrosis in combination with inflammatory cells, phagocytosis, and increased fibrous tissue. The frequency of necrotic fibers was significantly higher in the MI/HF+ group than in the sham. The MI/HF+ group had atrophy of type I, IC/IIC, and HA fibers compared to the sham group (P<0.05). MyoD gene expression was higher in the MI/HF+ group (sham: 1.00 +/- 0.49; MI/HF+: 2.53 +/- 0.71 arbitrary units; P<0.001). Myogenin and MRF4 gene expression was similar in both groups. Myogenin protein levels were reduced in the MI/HF+ group (sham: 1.00 +/- 0.21; MI/HF+: 0.74 +/- 0.21 arbitrary units; P=0.026). MyoD and MRF4 protein levels, as well as the MyHC distribution, were not different between groups. The MI/HF+ group had higher TNF-alpha and IL-6 serum concentrations than the sham group.Conclusions: Heart failure-induced skeletal muscle atrophy is combined with fiber necrosis, increased MyoD gene expression and decreased myogenin protein levels.
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spelling Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factorsmyocardial infarctionmyogenic regulatory factorsmyosin heavy chaincytokinesBackground: Although intrinsic skeletal muscle abnormalities can influence exercise intolerance during heart failure (HF), the factors responsible for muscle changes have not been elucidated. In this study we evaluated the expression of myogenic regulatory factors (MRF), myosin heavy chain (MyHC) isoforms, and fiber trophism in the soleus muscle of rats with myocardial infarction-induced heart failure.Method/Results: Six months after surgery, 2 groups of rats were studied: sham, and infarcted rats with HF (MI/HF+, MI size: 41.1 +/- 6.3% of total left ventricular area). In the infarcted group, microscopic evaluation revealed scattered foci of fiber necrosis in combination with inflammatory cells, phagocytosis, and increased fibrous tissue. The frequency of necrotic fibers was significantly higher in the MI/HF+ group than in the sham. The MI/HF+ group had atrophy of type I, IC/IIC, and HA fibers compared to the sham group (P<0.05). MyoD gene expression was higher in the MI/HF+ group (sham: 1.00 +/- 0.49; MI/HF+: 2.53 +/- 0.71 arbitrary units; P<0.001). Myogenin and MRF4 gene expression was similar in both groups. Myogenin protein levels were reduced in the MI/HF+ group (sham: 1.00 +/- 0.21; MI/HF+: 0.74 +/- 0.21 arbitrary units; P=0.026). MyoD and MRF4 protein levels, as well as the MyHC distribution, were not different between groups. The MI/HF+ group had higher TNF-alpha and IL-6 serum concentrations than the sham group.Conclusions: Heart failure-induced skeletal muscle atrophy is combined with fiber necrosis, increased MyoD gene expression and decreased myogenin protein levels.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Univ Estadual Paulista, Dept Internal Med, Botucatu Med Sch, São Paulo, BrazilUniv Estadual Paulista, Dept Morphol, Botucatu Biosci Inst, São Paulo, BrazilUniversidade Federal de Mato Grosso do Sul (UFMS), Sch Phys Therapy, Campo Grande, MS, BrazilUniv Estadual Paulista, Dept Biostat, Botucatu Biosci Inst, BR-18618000 São Paulo, BrazilUniv Estadual Paulista, Dept Internal Med, Botucatu Med Sch, São Paulo, BrazilUniv Estadual Paulista, Dept Morphol, Botucatu Biosci Inst, São Paulo, BrazilUniv Estadual Paulista, Dept Biostat, Botucatu Biosci Inst, BR-18618000 São Paulo, BrazilFAPESP: 05/58294-3FAPESP: 05/60767-7CNPq: 310547/2006-7FUNDUNESP: 00108/06-DFPInt Scientific Literature, IncUniversidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso do Sul (UFMS)Martinez, Paula F. [UNESP]Okoshi, Katashi [UNESP]Zornoff, Leonardo Antonio Mamede [UNESP]Carvalho, Robson F. [UNESP]Oliveira Junior, Silvio A. [UNESP]Lima, Aline R. R. [UNESP]Campos, Dijon H. S. [UNESP]Damatto, Ricardo L. [UNESP]Padovani, Carlos Roberto [UNESP]Nogueira, Célia Regina [UNESP]Pai-Silva, Maeli Dai [UNESP]Okoshi, Marina Politi [UNESP]2014-05-20T13:47:57Z2014-05-20T13:47:57Z2010-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBR374-BR383http://www.medscimonit.com/abstract/index/idArt/881289Medical Science Monitor. Albertson: Int Scientific Literature, Inc, v. 16, n. 12, p. BR374-BR383, 2010.1234-1010http://hdl.handle.net/11449/17086WOS:0002863617000042-s2.0-7995156236687278970805222894463138671998432159097157630942050168390153945470000-0002-4901-77140000-0001-8980-8839Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMedical Science Monitor0,619info:eu-repo/semantics/openAccess2024-08-14T17:22:59Zoai:repositorio.unesp.br:11449/17086Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors
title Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors
spellingShingle Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors
Martinez, Paula F. [UNESP]
myocardial infarction
myogenic regulatory factors
myosin heavy chain
cytokines
title_short Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors
title_full Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors
title_fullStr Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors
title_full_unstemmed Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors
title_sort Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors
author Martinez, Paula F. [UNESP]
author_facet Martinez, Paula F. [UNESP]
Okoshi, Katashi [UNESP]
Zornoff, Leonardo Antonio Mamede [UNESP]
Carvalho, Robson F. [UNESP]
Oliveira Junior, Silvio A. [UNESP]
Lima, Aline R. R. [UNESP]
Campos, Dijon H. S. [UNESP]
Damatto, Ricardo L. [UNESP]
Padovani, Carlos Roberto [UNESP]
Nogueira, Célia Regina [UNESP]
Pai-Silva, Maeli Dai [UNESP]
Okoshi, Marina Politi [UNESP]
author_role author
author2 Okoshi, Katashi [UNESP]
Zornoff, Leonardo Antonio Mamede [UNESP]
Carvalho, Robson F. [UNESP]
Oliveira Junior, Silvio A. [UNESP]
Lima, Aline R. R. [UNESP]
Campos, Dijon H. S. [UNESP]
Damatto, Ricardo L. [UNESP]
Padovani, Carlos Roberto [UNESP]
Nogueira, Célia Regina [UNESP]
Pai-Silva, Maeli Dai [UNESP]
Okoshi, Marina Politi [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.author.fl_str_mv Martinez, Paula F. [UNESP]
Okoshi, Katashi [UNESP]
Zornoff, Leonardo Antonio Mamede [UNESP]
Carvalho, Robson F. [UNESP]
Oliveira Junior, Silvio A. [UNESP]
Lima, Aline R. R. [UNESP]
Campos, Dijon H. S. [UNESP]
Damatto, Ricardo L. [UNESP]
Padovani, Carlos Roberto [UNESP]
Nogueira, Célia Regina [UNESP]
Pai-Silva, Maeli Dai [UNESP]
Okoshi, Marina Politi [UNESP]
dc.subject.por.fl_str_mv myocardial infarction
myogenic regulatory factors
myosin heavy chain
cytokines
topic myocardial infarction
myogenic regulatory factors
myosin heavy chain
cytokines
description Background: Although intrinsic skeletal muscle abnormalities can influence exercise intolerance during heart failure (HF), the factors responsible for muscle changes have not been elucidated. In this study we evaluated the expression of myogenic regulatory factors (MRF), myosin heavy chain (MyHC) isoforms, and fiber trophism in the soleus muscle of rats with myocardial infarction-induced heart failure.Method/Results: Six months after surgery, 2 groups of rats were studied: sham, and infarcted rats with HF (MI/HF+, MI size: 41.1 +/- 6.3% of total left ventricular area). In the infarcted group, microscopic evaluation revealed scattered foci of fiber necrosis in combination with inflammatory cells, phagocytosis, and increased fibrous tissue. The frequency of necrotic fibers was significantly higher in the MI/HF+ group than in the sham. The MI/HF+ group had atrophy of type I, IC/IIC, and HA fibers compared to the sham group (P<0.05). MyoD gene expression was higher in the MI/HF+ group (sham: 1.00 +/- 0.49; MI/HF+: 2.53 +/- 0.71 arbitrary units; P<0.001). Myogenin and MRF4 gene expression was similar in both groups. Myogenin protein levels were reduced in the MI/HF+ group (sham: 1.00 +/- 0.21; MI/HF+: 0.74 +/- 0.21 arbitrary units; P=0.026). MyoD and MRF4 protein levels, as well as the MyHC distribution, were not different between groups. The MI/HF+ group had higher TNF-alpha and IL-6 serum concentrations than the sham group.Conclusions: Heart failure-induced skeletal muscle atrophy is combined with fiber necrosis, increased MyoD gene expression and decreased myogenin protein levels.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-01
2014-05-20T13:47:57Z
2014-05-20T13:47:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.medscimonit.com/abstract/index/idArt/881289
Medical Science Monitor. Albertson: Int Scientific Literature, Inc, v. 16, n. 12, p. BR374-BR383, 2010.
1234-1010
http://hdl.handle.net/11449/17086
WOS:000286361700004
2-s2.0-79951562366
8727897080522289
4463138671998432
1590971576309420
5016839015394547
0000-0002-4901-7714
0000-0001-8980-8839
url http://www.medscimonit.com/abstract/index/idArt/881289
http://hdl.handle.net/11449/17086
identifier_str_mv Medical Science Monitor. Albertson: Int Scientific Literature, Inc, v. 16, n. 12, p. BR374-BR383, 2010.
1234-1010
WOS:000286361700004
2-s2.0-79951562366
8727897080522289
4463138671998432
1590971576309420
5016839015394547
0000-0002-4901-7714
0000-0001-8980-8839
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Medical Science Monitor
0,619
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv BR374-BR383
dc.publisher.none.fl_str_mv Int Scientific Literature, Inc
publisher.none.fl_str_mv Int Scientific Literature, Inc
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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