Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.medscimonit.com/abstract/index/idArt/881289 http://hdl.handle.net/11449/17086 |
Resumo: | Background: Although intrinsic skeletal muscle abnormalities can influence exercise intolerance during heart failure (HF), the factors responsible for muscle changes have not been elucidated. In this study we evaluated the expression of myogenic regulatory factors (MRF), myosin heavy chain (MyHC) isoforms, and fiber trophism in the soleus muscle of rats with myocardial infarction-induced heart failure.Method/Results: Six months after surgery, 2 groups of rats were studied: sham, and infarcted rats with HF (MI/HF+, MI size: 41.1 +/- 6.3% of total left ventricular area). In the infarcted group, microscopic evaluation revealed scattered foci of fiber necrosis in combination with inflammatory cells, phagocytosis, and increased fibrous tissue. The frequency of necrotic fibers was significantly higher in the MI/HF+ group than in the sham. The MI/HF+ group had atrophy of type I, IC/IIC, and HA fibers compared to the sham group (P<0.05). MyoD gene expression was higher in the MI/HF+ group (sham: 1.00 +/- 0.49; MI/HF+: 2.53 +/- 0.71 arbitrary units; P<0.001). Myogenin and MRF4 gene expression was similar in both groups. Myogenin protein levels were reduced in the MI/HF+ group (sham: 1.00 +/- 0.21; MI/HF+: 0.74 +/- 0.21 arbitrary units; P=0.026). MyoD and MRF4 protein levels, as well as the MyHC distribution, were not different between groups. The MI/HF+ group had higher TNF-alpha and IL-6 serum concentrations than the sham group.Conclusions: Heart failure-induced skeletal muscle atrophy is combined with fiber necrosis, increased MyoD gene expression and decreased myogenin protein levels. |
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Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factorsmyocardial infarctionmyogenic regulatory factorsmyosin heavy chaincytokinesBackground: Although intrinsic skeletal muscle abnormalities can influence exercise intolerance during heart failure (HF), the factors responsible for muscle changes have not been elucidated. In this study we evaluated the expression of myogenic regulatory factors (MRF), myosin heavy chain (MyHC) isoforms, and fiber trophism in the soleus muscle of rats with myocardial infarction-induced heart failure.Method/Results: Six months after surgery, 2 groups of rats were studied: sham, and infarcted rats with HF (MI/HF+, MI size: 41.1 +/- 6.3% of total left ventricular area). In the infarcted group, microscopic evaluation revealed scattered foci of fiber necrosis in combination with inflammatory cells, phagocytosis, and increased fibrous tissue. The frequency of necrotic fibers was significantly higher in the MI/HF+ group than in the sham. The MI/HF+ group had atrophy of type I, IC/IIC, and HA fibers compared to the sham group (P<0.05). MyoD gene expression was higher in the MI/HF+ group (sham: 1.00 +/- 0.49; MI/HF+: 2.53 +/- 0.71 arbitrary units; P<0.001). Myogenin and MRF4 gene expression was similar in both groups. Myogenin protein levels were reduced in the MI/HF+ group (sham: 1.00 +/- 0.21; MI/HF+: 0.74 +/- 0.21 arbitrary units; P=0.026). MyoD and MRF4 protein levels, as well as the MyHC distribution, were not different between groups. The MI/HF+ group had higher TNF-alpha and IL-6 serum concentrations than the sham group.Conclusions: Heart failure-induced skeletal muscle atrophy is combined with fiber necrosis, increased MyoD gene expression and decreased myogenin protein levels.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Univ Estadual Paulista, Dept Internal Med, Botucatu Med Sch, São Paulo, BrazilUniv Estadual Paulista, Dept Morphol, Botucatu Biosci Inst, São Paulo, BrazilUniversidade Federal de Mato Grosso do Sul (UFMS), Sch Phys Therapy, Campo Grande, MS, BrazilUniv Estadual Paulista, Dept Biostat, Botucatu Biosci Inst, BR-18618000 São Paulo, BrazilUniv Estadual Paulista, Dept Internal Med, Botucatu Med Sch, São Paulo, BrazilUniv Estadual Paulista, Dept Morphol, Botucatu Biosci Inst, São Paulo, BrazilUniv Estadual Paulista, Dept Biostat, Botucatu Biosci Inst, BR-18618000 São Paulo, BrazilFAPESP: 05/58294-3FAPESP: 05/60767-7CNPq: 310547/2006-7FUNDUNESP: 00108/06-DFPInt Scientific Literature, IncUniversidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso do Sul (UFMS)Martinez, Paula F. [UNESP]Okoshi, Katashi [UNESP]Zornoff, Leonardo Antonio Mamede [UNESP]Carvalho, Robson F. [UNESP]Oliveira Junior, Silvio A. [UNESP]Lima, Aline R. R. [UNESP]Campos, Dijon H. S. [UNESP]Damatto, Ricardo L. [UNESP]Padovani, Carlos Roberto [UNESP]Nogueira, Célia Regina [UNESP]Pai-Silva, Maeli Dai [UNESP]Okoshi, Marina Politi [UNESP]2014-05-20T13:47:57Z2014-05-20T13:47:57Z2010-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBR374-BR383http://www.medscimonit.com/abstract/index/idArt/881289Medical Science Monitor. Albertson: Int Scientific Literature, Inc, v. 16, n. 12, p. BR374-BR383, 2010.1234-1010http://hdl.handle.net/11449/17086WOS:0002863617000042-s2.0-7995156236687278970805222894463138671998432159097157630942050168390153945470000-0002-4901-77140000-0001-8980-8839Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMedical Science Monitor0,619info:eu-repo/semantics/openAccess2024-08-14T17:22:59Zoai:repositorio.unesp.br:11449/17086Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors |
title |
Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors |
spellingShingle |
Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors Martinez, Paula F. [UNESP] myocardial infarction myogenic regulatory factors myosin heavy chain cytokines |
title_short |
Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors |
title_full |
Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors |
title_fullStr |
Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors |
title_full_unstemmed |
Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors |
title_sort |
Chronic heart failure-induced skeletal muscle atrophy, necrosis, and changes in myogenic regulatiory factors |
author |
Martinez, Paula F. [UNESP] |
author_facet |
Martinez, Paula F. [UNESP] Okoshi, Katashi [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] Carvalho, Robson F. [UNESP] Oliveira Junior, Silvio A. [UNESP] Lima, Aline R. R. [UNESP] Campos, Dijon H. S. [UNESP] Damatto, Ricardo L. [UNESP] Padovani, Carlos Roberto [UNESP] Nogueira, Célia Regina [UNESP] Pai-Silva, Maeli Dai [UNESP] Okoshi, Marina Politi [UNESP] |
author_role |
author |
author2 |
Okoshi, Katashi [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] Carvalho, Robson F. [UNESP] Oliveira Junior, Silvio A. [UNESP] Lima, Aline R. R. [UNESP] Campos, Dijon H. S. [UNESP] Damatto, Ricardo L. [UNESP] Padovani, Carlos Roberto [UNESP] Nogueira, Célia Regina [UNESP] Pai-Silva, Maeli Dai [UNESP] Okoshi, Marina Politi [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Mato Grosso do Sul (UFMS) |
dc.contributor.author.fl_str_mv |
Martinez, Paula F. [UNESP] Okoshi, Katashi [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] Carvalho, Robson F. [UNESP] Oliveira Junior, Silvio A. [UNESP] Lima, Aline R. R. [UNESP] Campos, Dijon H. S. [UNESP] Damatto, Ricardo L. [UNESP] Padovani, Carlos Roberto [UNESP] Nogueira, Célia Regina [UNESP] Pai-Silva, Maeli Dai [UNESP] Okoshi, Marina Politi [UNESP] |
dc.subject.por.fl_str_mv |
myocardial infarction myogenic regulatory factors myosin heavy chain cytokines |
topic |
myocardial infarction myogenic regulatory factors myosin heavy chain cytokines |
description |
Background: Although intrinsic skeletal muscle abnormalities can influence exercise intolerance during heart failure (HF), the factors responsible for muscle changes have not been elucidated. In this study we evaluated the expression of myogenic regulatory factors (MRF), myosin heavy chain (MyHC) isoforms, and fiber trophism in the soleus muscle of rats with myocardial infarction-induced heart failure.Method/Results: Six months after surgery, 2 groups of rats were studied: sham, and infarcted rats with HF (MI/HF+, MI size: 41.1 +/- 6.3% of total left ventricular area). In the infarcted group, microscopic evaluation revealed scattered foci of fiber necrosis in combination with inflammatory cells, phagocytosis, and increased fibrous tissue. The frequency of necrotic fibers was significantly higher in the MI/HF+ group than in the sham. The MI/HF+ group had atrophy of type I, IC/IIC, and HA fibers compared to the sham group (P<0.05). MyoD gene expression was higher in the MI/HF+ group (sham: 1.00 +/- 0.49; MI/HF+: 2.53 +/- 0.71 arbitrary units; P<0.001). Myogenin and MRF4 gene expression was similar in both groups. Myogenin protein levels were reduced in the MI/HF+ group (sham: 1.00 +/- 0.21; MI/HF+: 0.74 +/- 0.21 arbitrary units; P=0.026). MyoD and MRF4 protein levels, as well as the MyHC distribution, were not different between groups. The MI/HF+ group had higher TNF-alpha and IL-6 serum concentrations than the sham group.Conclusions: Heart failure-induced skeletal muscle atrophy is combined with fiber necrosis, increased MyoD gene expression and decreased myogenin protein levels. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12-01 2014-05-20T13:47:57Z 2014-05-20T13:47:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.medscimonit.com/abstract/index/idArt/881289 Medical Science Monitor. Albertson: Int Scientific Literature, Inc, v. 16, n. 12, p. BR374-BR383, 2010. 1234-1010 http://hdl.handle.net/11449/17086 WOS:000286361700004 2-s2.0-79951562366 8727897080522289 4463138671998432 1590971576309420 5016839015394547 0000-0002-4901-7714 0000-0001-8980-8839 |
url |
http://www.medscimonit.com/abstract/index/idArt/881289 http://hdl.handle.net/11449/17086 |
identifier_str_mv |
Medical Science Monitor. Albertson: Int Scientific Literature, Inc, v. 16, n. 12, p. BR374-BR383, 2010. 1234-1010 WOS:000286361700004 2-s2.0-79951562366 8727897080522289 4463138671998432 1590971576309420 5016839015394547 0000-0002-4901-7714 0000-0001-8980-8839 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Medical Science Monitor 0,619 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
BR374-BR383 |
dc.publisher.none.fl_str_mv |
Int Scientific Literature, Inc |
publisher.none.fl_str_mv |
Int Scientific Literature, Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128142391377920 |