Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1756-0500-3-260 http://hdl.handle.net/11449/71928 |
Resumo: | Background: The ability of biofilm formation seems to play an essential role in the virulence of coagulase-negative staphylococci (CNS). The most clearly characterized component of staphylococcal biofilms is the polysaccharide intercellular adhesin (PIA) encoded by the icaADBC operon. Biofilm production was studied in 80 coagulase-negative staphylococci (CNS) strains isolated from clinical specimens of newborns with infection hospitalized at the Neonatal Unit of the University Hospital, Faculty of Medicine of Botucatu, and in 20 isolates obtained from the nares of healthy individuals without signs of infection. The objective was to compare three phenotypic methods with the detection of the icaA, icaD and icaC genes by PCR. Findings: Among the 100 CNS isolates studied, 82% tested positive by PCR, 82% by the tube test, 81% by the TCP assay, and 73% by the CRA method. Using PCR as a reference, the tube test showed the best correlation with detection of the ica genes, presenting high sensitivity and specificity. Conclusions: The tube adherence test can be indicated for the routine detection of biofilm production in CNS because of its easy application and low cost and because it guarantees reliable results with excellent sensitivity and specificity. © 2010 Cunha et al; licensee BioMed Central Ltd. |
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Repositório Institucional da UNESP |
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Comparison of methods for the detection of biofilm production in coagulase-negative staphylococciBackground: The ability of biofilm formation seems to play an essential role in the virulence of coagulase-negative staphylococci (CNS). The most clearly characterized component of staphylococcal biofilms is the polysaccharide intercellular adhesin (PIA) encoded by the icaADBC operon. Biofilm production was studied in 80 coagulase-negative staphylococci (CNS) strains isolated from clinical specimens of newborns with infection hospitalized at the Neonatal Unit of the University Hospital, Faculty of Medicine of Botucatu, and in 20 isolates obtained from the nares of healthy individuals without signs of infection. The objective was to compare three phenotypic methods with the detection of the icaA, icaD and icaC genes by PCR. Findings: Among the 100 CNS isolates studied, 82% tested positive by PCR, 82% by the tube test, 81% by the TCP assay, and 73% by the CRA method. Using PCR as a reference, the tube test showed the best correlation with detection of the ica genes, presenting high sensitivity and specificity. Conclusions: The tube adherence test can be indicated for the routine detection of biofilm production in CNS because of its easy application and low cost and because it guarantees reliable results with excellent sensitivity and specificity. © 2010 Cunha et al; licensee BioMed Central Ltd.UNESP-Univ Estadual Paulista Department of Microbiology and Immunology Biosciences Institute Bacteriology Laboratory, Botucatu, SPUNESP-Univ Estadual Paulista Department of Microbiology and Immunology Biosciences Institute Bacteriology Laboratory, Botucatu, SPUniversidade Estadual Paulista (Unesp)Oliveira, Adilson [UNESP]Cunha, Maria de Lourdes R. S. [UNESP]2014-05-27T11:24:49Z2014-05-27T11:24:49Z2010-10-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/1756-0500-3-260BMC Research Notes, v. 3.1756-0500http://hdl.handle.net/11449/7192810.1186/1756-0500-3-2602-s2.0-779578087042-s2.0-77957808704.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Research Notes0,691info:eu-repo/semantics/openAccess2023-10-23T06:04:15Zoai:repositorio.unesp.br:11449/71928Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-23T06:04:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci |
title |
Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci |
spellingShingle |
Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci Oliveira, Adilson [UNESP] |
title_short |
Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci |
title_full |
Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci |
title_fullStr |
Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci |
title_full_unstemmed |
Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci |
title_sort |
Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci |
author |
Oliveira, Adilson [UNESP] |
author_facet |
Oliveira, Adilson [UNESP] Cunha, Maria de Lourdes R. S. [UNESP] |
author_role |
author |
author2 |
Cunha, Maria de Lourdes R. S. [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Oliveira, Adilson [UNESP] Cunha, Maria de Lourdes R. S. [UNESP] |
description |
Background: The ability of biofilm formation seems to play an essential role in the virulence of coagulase-negative staphylococci (CNS). The most clearly characterized component of staphylococcal biofilms is the polysaccharide intercellular adhesin (PIA) encoded by the icaADBC operon. Biofilm production was studied in 80 coagulase-negative staphylococci (CNS) strains isolated from clinical specimens of newborns with infection hospitalized at the Neonatal Unit of the University Hospital, Faculty of Medicine of Botucatu, and in 20 isolates obtained from the nares of healthy individuals without signs of infection. The objective was to compare three phenotypic methods with the detection of the icaA, icaD and icaC genes by PCR. Findings: Among the 100 CNS isolates studied, 82% tested positive by PCR, 82% by the tube test, 81% by the TCP assay, and 73% by the CRA method. Using PCR as a reference, the tube test showed the best correlation with detection of the ica genes, presenting high sensitivity and specificity. Conclusions: The tube adherence test can be indicated for the routine detection of biofilm production in CNS because of its easy application and low cost and because it guarantees reliable results with excellent sensitivity and specificity. © 2010 Cunha et al; licensee BioMed Central Ltd. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10-18 2014-05-27T11:24:49Z 2014-05-27T11:24:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1756-0500-3-260 BMC Research Notes, v. 3. 1756-0500 http://hdl.handle.net/11449/71928 10.1186/1756-0500-3-260 2-s2.0-77957808704 2-s2.0-77957808704.pdf |
url |
http://dx.doi.org/10.1186/1756-0500-3-260 http://hdl.handle.net/11449/71928 |
identifier_str_mv |
BMC Research Notes, v. 3. 1756-0500 10.1186/1756-0500-3-260 2-s2.0-77957808704 2-s2.0-77957808704.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Research Notes 0,691 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964662978052096 |