Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.micpath.2018.11.009 http://hdl.handle.net/11449/185423 |
Resumo: | The search for new antimicrobial drugs has been necessary due to the increased bacterial resistance to antibiotics currently in use, and natural products play an important role in this field. The aim of this study was to evaluate the in vitro effect of cinnamaldehyde on S. epidermidis strains, biofilm set-up prevention, as well as its effect on pre-established biofilms. The minimum inhibitory concentration (MIC) ranged from 300 to 500 mu g/mL, and the minimum bactericidal concentration (MBC) from 400 to 600 mu g/mL. The biofilm inhibitory concentration and biofilm eradication concentration values were four-fold (clinical isolate) and eight-fold (ATCC strain) greater than the concentration required to inhibit planktonic growth. Sub-inhibitory concentrations of cinnamaldehyde attenuated biofilm formation of S. epidermidis strains on polystyrene microtiter plates. The combination of cinnamaldehyde and linezolid was able to inhibit S. epidermidis with a bactericidal effect. Further investigation of the mechanism of action of cinnamaldehyde revealed its effect on the cell membrane permeability, and confocal laser scanning microscopy (CLSM) images illustrated the impact of cinnamaldehyde in the detachment and killing of existing biofilms. Thereby, our data confirmed the ability of cinnamaldehyde to reduce bacterial planktonic growth of S. epidermidis, inhibiting biofilm formation and eradicating pre-formed biofilm. |
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Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidisBacterial resistanceConfocal laser scanning microscopyNatural productsSynergismBiofilmStaphylococcus epidermidisThe search for new antimicrobial drugs has been necessary due to the increased bacterial resistance to antibiotics currently in use, and natural products play an important role in this field. The aim of this study was to evaluate the in vitro effect of cinnamaldehyde on S. epidermidis strains, biofilm set-up prevention, as well as its effect on pre-established biofilms. The minimum inhibitory concentration (MIC) ranged from 300 to 500 mu g/mL, and the minimum bactericidal concentration (MBC) from 400 to 600 mu g/mL. The biofilm inhibitory concentration and biofilm eradication concentration values were four-fold (clinical isolate) and eight-fold (ATCC strain) greater than the concentration required to inhibit planktonic growth. Sub-inhibitory concentrations of cinnamaldehyde attenuated biofilm formation of S. epidermidis strains on polystyrene microtiter plates. The combination of cinnamaldehyde and linezolid was able to inhibit S. epidermidis with a bactericidal effect. Further investigation of the mechanism of action of cinnamaldehyde revealed its effect on the cell membrane permeability, and confocal laser scanning microscopy (CLSM) images illustrated the impact of cinnamaldehyde in the detachment and killing of existing biofilms. Thereby, our data confirmed the ability of cinnamaldehyde to reduce bacterial planktonic growth of S. epidermidis, inhibiting biofilm formation and eradicating pre-formed biofilm.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Dept Microbiol & Immunol, BR-18618691 Botucatu, SP, BrazilUniv Estadual Paulista, Dept Chem & Biochem, BR-18618693 Botucatu, SP, BrazilUniv Paranaense, Anim Sci Postgrad Program, BR-87502210 Curitiba, Parana, BrazilUniv Estadual Paulista, Dept Microbiol & Immunol, BR-18618691 Botucatu, SP, BrazilUniv Estadual Paulista, Dept Chem & Biochem, BR-18618693 Botucatu, SP, BrazilFAPESP: 2015/14278-6CNPq: 150763/2017-4Elsevier B.V.Universidade Estadual Paulista (Unesp)Univ ParanaenseAlbano, Mariana [UNESP]Crulhas, Bruno Pereira [UNESP]Bergamo Alves, Fernanda Cristina [UNESP]Marques Pereira, Ana Flavia [UNESP]Murbach Teles Andrade, Bruna Fernanda [UNESP]Barbosa, Lidiane NunesFurlanetto, Alessandra [UNESP]Silveira Lyra, Luciana Pupo da [UNESP]Mores Rall, Vera Lucia [UNESP]Fernandes Junior, Ary [UNESP]2019-10-04T12:35:18Z2019-10-04T12:35:18Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article231-238http://dx.doi.org/10.1016/j.micpath.2018.11.009Microbial Pathogenesis. London: Academic Press Ltd- Elsevier Science Ltd, v. 126, p. 231-238, 2019.0882-4010http://hdl.handle.net/11449/18542310.1016/j.micpath.2018.11.009WOS:000458468100031Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Pathogenesisinfo:eu-repo/semantics/openAccess2021-10-23T19:28:29Zoai:repositorio.unesp.br:11449/185423Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:28:47.671389Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis |
title |
Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis |
spellingShingle |
Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis Albano, Mariana [UNESP] Bacterial resistance Confocal laser scanning microscopy Natural products Synergism Biofilm Staphylococcus epidermidis |
title_short |
Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis |
title_full |
Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis |
title_fullStr |
Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis |
title_full_unstemmed |
Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis |
title_sort |
Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis |
author |
Albano, Mariana [UNESP] |
author_facet |
Albano, Mariana [UNESP] Crulhas, Bruno Pereira [UNESP] Bergamo Alves, Fernanda Cristina [UNESP] Marques Pereira, Ana Flavia [UNESP] Murbach Teles Andrade, Bruna Fernanda [UNESP] Barbosa, Lidiane Nunes Furlanetto, Alessandra [UNESP] Silveira Lyra, Luciana Pupo da [UNESP] Mores Rall, Vera Lucia [UNESP] Fernandes Junior, Ary [UNESP] |
author_role |
author |
author2 |
Crulhas, Bruno Pereira [UNESP] Bergamo Alves, Fernanda Cristina [UNESP] Marques Pereira, Ana Flavia [UNESP] Murbach Teles Andrade, Bruna Fernanda [UNESP] Barbosa, Lidiane Nunes Furlanetto, Alessandra [UNESP] Silveira Lyra, Luciana Pupo da [UNESP] Mores Rall, Vera Lucia [UNESP] Fernandes Junior, Ary [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Paranaense |
dc.contributor.author.fl_str_mv |
Albano, Mariana [UNESP] Crulhas, Bruno Pereira [UNESP] Bergamo Alves, Fernanda Cristina [UNESP] Marques Pereira, Ana Flavia [UNESP] Murbach Teles Andrade, Bruna Fernanda [UNESP] Barbosa, Lidiane Nunes Furlanetto, Alessandra [UNESP] Silveira Lyra, Luciana Pupo da [UNESP] Mores Rall, Vera Lucia [UNESP] Fernandes Junior, Ary [UNESP] |
dc.subject.por.fl_str_mv |
Bacterial resistance Confocal laser scanning microscopy Natural products Synergism Biofilm Staphylococcus epidermidis |
topic |
Bacterial resistance Confocal laser scanning microscopy Natural products Synergism Biofilm Staphylococcus epidermidis |
description |
The search for new antimicrobial drugs has been necessary due to the increased bacterial resistance to antibiotics currently in use, and natural products play an important role in this field. The aim of this study was to evaluate the in vitro effect of cinnamaldehyde on S. epidermidis strains, biofilm set-up prevention, as well as its effect on pre-established biofilms. The minimum inhibitory concentration (MIC) ranged from 300 to 500 mu g/mL, and the minimum bactericidal concentration (MBC) from 400 to 600 mu g/mL. The biofilm inhibitory concentration and biofilm eradication concentration values were four-fold (clinical isolate) and eight-fold (ATCC strain) greater than the concentration required to inhibit planktonic growth. Sub-inhibitory concentrations of cinnamaldehyde attenuated biofilm formation of S. epidermidis strains on polystyrene microtiter plates. The combination of cinnamaldehyde and linezolid was able to inhibit S. epidermidis with a bactericidal effect. Further investigation of the mechanism of action of cinnamaldehyde revealed its effect on the cell membrane permeability, and confocal laser scanning microscopy (CLSM) images illustrated the impact of cinnamaldehyde in the detachment and killing of existing biofilms. Thereby, our data confirmed the ability of cinnamaldehyde to reduce bacterial planktonic growth of S. epidermidis, inhibiting biofilm formation and eradicating pre-formed biofilm. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:35:18Z 2019-10-04T12:35:18Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.micpath.2018.11.009 Microbial Pathogenesis. London: Academic Press Ltd- Elsevier Science Ltd, v. 126, p. 231-238, 2019. 0882-4010 http://hdl.handle.net/11449/185423 10.1016/j.micpath.2018.11.009 WOS:000458468100031 |
url |
http://dx.doi.org/10.1016/j.micpath.2018.11.009 http://hdl.handle.net/11449/185423 |
identifier_str_mv |
Microbial Pathogenesis. London: Academic Press Ltd- Elsevier Science Ltd, v. 126, p. 231-238, 2019. 0882-4010 10.1016/j.micpath.2018.11.009 WOS:000458468100031 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microbial Pathogenesis |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
231-238 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128816406593536 |