Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis

Detalhes bibliográficos
Autor(a) principal: Albano, Mariana [UNESP]
Data de Publicação: 2019
Outros Autores: Crulhas, Bruno Pereira [UNESP], Bergamo Alves, Fernanda Cristina [UNESP], Marques Pereira, Ana Flavia [UNESP], Murbach Teles Andrade, Bruna Fernanda [UNESP], Barbosa, Lidiane Nunes, Furlanetto, Alessandra [UNESP], Silveira Lyra, Luciana Pupo da [UNESP], Mores Rall, Vera Lucia [UNESP], Fernandes Junior, Ary [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.micpath.2018.11.009
http://hdl.handle.net/11449/185423
Resumo: The search for new antimicrobial drugs has been necessary due to the increased bacterial resistance to antibiotics currently in use, and natural products play an important role in this field. The aim of this study was to evaluate the in vitro effect of cinnamaldehyde on S. epidermidis strains, biofilm set-up prevention, as well as its effect on pre-established biofilms. The minimum inhibitory concentration (MIC) ranged from 300 to 500 mu g/mL, and the minimum bactericidal concentration (MBC) from 400 to 600 mu g/mL. The biofilm inhibitory concentration and biofilm eradication concentration values were four-fold (clinical isolate) and eight-fold (ATCC strain) greater than the concentration required to inhibit planktonic growth. Sub-inhibitory concentrations of cinnamaldehyde attenuated biofilm formation of S. epidermidis strains on polystyrene microtiter plates. The combination of cinnamaldehyde and linezolid was able to inhibit S. epidermidis with a bactericidal effect. Further investigation of the mechanism of action of cinnamaldehyde revealed its effect on the cell membrane permeability, and confocal laser scanning microscopy (CLSM) images illustrated the impact of cinnamaldehyde in the detachment and killing of existing biofilms. Thereby, our data confirmed the ability of cinnamaldehyde to reduce bacterial planktonic growth of S. epidermidis, inhibiting biofilm formation and eradicating pre-formed biofilm.
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spelling Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidisBacterial resistanceConfocal laser scanning microscopyNatural productsSynergismBiofilmStaphylococcus epidermidisThe search for new antimicrobial drugs has been necessary due to the increased bacterial resistance to antibiotics currently in use, and natural products play an important role in this field. The aim of this study was to evaluate the in vitro effect of cinnamaldehyde on S. epidermidis strains, biofilm set-up prevention, as well as its effect on pre-established biofilms. The minimum inhibitory concentration (MIC) ranged from 300 to 500 mu g/mL, and the minimum bactericidal concentration (MBC) from 400 to 600 mu g/mL. The biofilm inhibitory concentration and biofilm eradication concentration values were four-fold (clinical isolate) and eight-fold (ATCC strain) greater than the concentration required to inhibit planktonic growth. Sub-inhibitory concentrations of cinnamaldehyde attenuated biofilm formation of S. epidermidis strains on polystyrene microtiter plates. The combination of cinnamaldehyde and linezolid was able to inhibit S. epidermidis with a bactericidal effect. Further investigation of the mechanism of action of cinnamaldehyde revealed its effect on the cell membrane permeability, and confocal laser scanning microscopy (CLSM) images illustrated the impact of cinnamaldehyde in the detachment and killing of existing biofilms. Thereby, our data confirmed the ability of cinnamaldehyde to reduce bacterial planktonic growth of S. epidermidis, inhibiting biofilm formation and eradicating pre-formed biofilm.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Dept Microbiol & Immunol, BR-18618691 Botucatu, SP, BrazilUniv Estadual Paulista, Dept Chem & Biochem, BR-18618693 Botucatu, SP, BrazilUniv Paranaense, Anim Sci Postgrad Program, BR-87502210 Curitiba, Parana, BrazilUniv Estadual Paulista, Dept Microbiol & Immunol, BR-18618691 Botucatu, SP, BrazilUniv Estadual Paulista, Dept Chem & Biochem, BR-18618693 Botucatu, SP, BrazilFAPESP: 2015/14278-6CNPq: 150763/2017-4Elsevier B.V.Universidade Estadual Paulista (Unesp)Univ ParanaenseAlbano, Mariana [UNESP]Crulhas, Bruno Pereira [UNESP]Bergamo Alves, Fernanda Cristina [UNESP]Marques Pereira, Ana Flavia [UNESP]Murbach Teles Andrade, Bruna Fernanda [UNESP]Barbosa, Lidiane NunesFurlanetto, Alessandra [UNESP]Silveira Lyra, Luciana Pupo da [UNESP]Mores Rall, Vera Lucia [UNESP]Fernandes Junior, Ary [UNESP]2019-10-04T12:35:18Z2019-10-04T12:35:18Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article231-238http://dx.doi.org/10.1016/j.micpath.2018.11.009Microbial Pathogenesis. London: Academic Press Ltd- Elsevier Science Ltd, v. 126, p. 231-238, 2019.0882-4010http://hdl.handle.net/11449/18542310.1016/j.micpath.2018.11.009WOS:000458468100031Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Pathogenesisinfo:eu-repo/semantics/openAccess2021-10-23T19:28:29Zoai:repositorio.unesp.br:11449/185423Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:28:47.671389Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis
title Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis
spellingShingle Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis
Albano, Mariana [UNESP]
Bacterial resistance
Confocal laser scanning microscopy
Natural products
Synergism
Biofilm
Staphylococcus epidermidis
title_short Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis
title_full Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis
title_fullStr Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis
title_full_unstemmed Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis
title_sort Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis
author Albano, Mariana [UNESP]
author_facet Albano, Mariana [UNESP]
Crulhas, Bruno Pereira [UNESP]
Bergamo Alves, Fernanda Cristina [UNESP]
Marques Pereira, Ana Flavia [UNESP]
Murbach Teles Andrade, Bruna Fernanda [UNESP]
Barbosa, Lidiane Nunes
Furlanetto, Alessandra [UNESP]
Silveira Lyra, Luciana Pupo da [UNESP]
Mores Rall, Vera Lucia [UNESP]
Fernandes Junior, Ary [UNESP]
author_role author
author2 Crulhas, Bruno Pereira [UNESP]
Bergamo Alves, Fernanda Cristina [UNESP]
Marques Pereira, Ana Flavia [UNESP]
Murbach Teles Andrade, Bruna Fernanda [UNESP]
Barbosa, Lidiane Nunes
Furlanetto, Alessandra [UNESP]
Silveira Lyra, Luciana Pupo da [UNESP]
Mores Rall, Vera Lucia [UNESP]
Fernandes Junior, Ary [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ Paranaense
dc.contributor.author.fl_str_mv Albano, Mariana [UNESP]
Crulhas, Bruno Pereira [UNESP]
Bergamo Alves, Fernanda Cristina [UNESP]
Marques Pereira, Ana Flavia [UNESP]
Murbach Teles Andrade, Bruna Fernanda [UNESP]
Barbosa, Lidiane Nunes
Furlanetto, Alessandra [UNESP]
Silveira Lyra, Luciana Pupo da [UNESP]
Mores Rall, Vera Lucia [UNESP]
Fernandes Junior, Ary [UNESP]
dc.subject.por.fl_str_mv Bacterial resistance
Confocal laser scanning microscopy
Natural products
Synergism
Biofilm
Staphylococcus epidermidis
topic Bacterial resistance
Confocal laser scanning microscopy
Natural products
Synergism
Biofilm
Staphylococcus epidermidis
description The search for new antimicrobial drugs has been necessary due to the increased bacterial resistance to antibiotics currently in use, and natural products play an important role in this field. The aim of this study was to evaluate the in vitro effect of cinnamaldehyde on S. epidermidis strains, biofilm set-up prevention, as well as its effect on pre-established biofilms. The minimum inhibitory concentration (MIC) ranged from 300 to 500 mu g/mL, and the minimum bactericidal concentration (MBC) from 400 to 600 mu g/mL. The biofilm inhibitory concentration and biofilm eradication concentration values were four-fold (clinical isolate) and eight-fold (ATCC strain) greater than the concentration required to inhibit planktonic growth. Sub-inhibitory concentrations of cinnamaldehyde attenuated biofilm formation of S. epidermidis strains on polystyrene microtiter plates. The combination of cinnamaldehyde and linezolid was able to inhibit S. epidermidis with a bactericidal effect. Further investigation of the mechanism of action of cinnamaldehyde revealed its effect on the cell membrane permeability, and confocal laser scanning microscopy (CLSM) images illustrated the impact of cinnamaldehyde in the detachment and killing of existing biofilms. Thereby, our data confirmed the ability of cinnamaldehyde to reduce bacterial planktonic growth of S. epidermidis, inhibiting biofilm formation and eradicating pre-formed biofilm.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-04T12:35:18Z
2019-10-04T12:35:18Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.micpath.2018.11.009
Microbial Pathogenesis. London: Academic Press Ltd- Elsevier Science Ltd, v. 126, p. 231-238, 2019.
0882-4010
http://hdl.handle.net/11449/185423
10.1016/j.micpath.2018.11.009
WOS:000458468100031
url http://dx.doi.org/10.1016/j.micpath.2018.11.009
http://hdl.handle.net/11449/185423
identifier_str_mv Microbial Pathogenesis. London: Academic Press Ltd- Elsevier Science Ltd, v. 126, p. 231-238, 2019.
0882-4010
10.1016/j.micpath.2018.11.009
WOS:000458468100031
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microbial Pathogenesis
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 231-238
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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