Preparation of an affinity cryogel column for lysozyme purification

• Autor(a) principal: Veríssimo, Lizzy Ayra Alcântara
• Data de Publicação: 2017
• Outros Autores: Paganoto, Fernanda Spagnol, Mol, Paula Chequer Gouveia [UNESP], Ilhéu Fontan, R. d. C., Minim, Valéria Paula Rodrigues, Minim, Luis Antonio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1080/01496395.2017.1318921; http://hdl.handle.net/11449/169760
• topic: Adsorption; affinity chromatography; cryogel; lysozyme; Tris

Affinity cryogels were synthesized using tris(hydroxymethyl)aminomethane (Tris) as ligand for specific interactions with the lysozyme (LYZ). The cryogel was produced by cryo-copolymerization at −12°C. A central composite rotational design 22 was used to optimize the immobilization procedure of the Tris on the cryogel. A maximum adsorption for LYZ (149.5 mg/g) was achieved when 376 mg/mL of Tris and 3.06 mol/L of sodium borohydride were used during the Tris immobilization. Chromatographic separation of LYZ from chicken egg white was done with a purity of 92.13%. Results showed that the affinity cryogel was a potential separation medium for LYZ purification.