Antimicrobial potential, phytochemical profile, cytotoxic and genotoxic screening of sedum praealtum a. DC. (balsam)
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12906-020-02915-6 http://hdl.handle.net/11449/200580 |
Resumo: | Background: Sedum praealtum has been used for a long time in traditional medicine as an analgesic and antiinflammatory agent. Its beneficial effects have been known since ancient times, when Latinos used it to treat sore and swollen eyes. This research evaluated the antimicrobial potential, the cytotoxic and genotoxic effects, and some chromatographic profiles of the hydroethanolic extract of leaves, stems and roots of S. praealtum. Methods: The antimicrobial activities were carried out by broth microdilution and agar diffusion. In vitro cytotoxicity was evaluated by cell cultures of Aedes albopictus and the selectivity index (SI) was estimated: SI=CI50/MIC. Genotoxic and systemic toxic effects of S. praealtum leaves were analyzed by micronucleus assay in mice bone marrow. Chromatographic profiles and mass spectra were investigated by GC-MS. Results: Gram-positive (B. subtilis, B. cereus, M. luteus, E. faecalis and S. aureus) and gram-negative (E. coli, E. aerogenes, S. marcescens, P. aeruginosa, P. mirabilis and S. typhimurium) bacteria exhibited MICs ranging from 12.5–50 and 0–50 mg/ ml, respectively. Sedum praealtum showed no efficacy against M. tuberculosis and M. bovis. Cytotoxicity (CI50) of S. praealtum was 4.22 and 5.96 mg/ml for leaves and stems, respectively, while its roots showed no cytotoxicity. Micronucleated polychromatic erythrocytes (MNPCEs) analyzes showed no differences between treatment doses (0.5–2 g/kg) and negative control (NaCl), but the PCE/NCE ratio (polychromatic erythrocyte/normochromatic erythrocyte) showed significant differences. Phytochemical screening identified thirteen compounds in the leaves, stems and roots of S. praealtum potentially associated with their biological activities. Conclusions: This research comprises a first scientific study on genotoxicity, cytotoxicity and antimicrobial effects of S. praealtum (Balsam), and it provides an initial theoretical foundation for its comprehensive use. Results showed antibacterial action of S. praealtum against gram-positive bacteria and some gram-negative species (depending on the plant anatomical part), but ineffective antimycobacterial action. However, S. praealtum leaves and stems display potential cytotoxicity, contributing to the SI < 1 values. In addition, S. praealtum leaves exhibit no clastogenic and/or aneugenic effects, but it has systemic toxicity dose-independent. |
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Antimicrobial potential, phytochemical profile, cytotoxic and genotoxic screening of sedum praealtum a. DC. (balsam)Antimicrobial susceptibilityCytotoxicityGenotoxicityPhytochemical screeningSedum praealtum a. DCBackground: Sedum praealtum has been used for a long time in traditional medicine as an analgesic and antiinflammatory agent. Its beneficial effects have been known since ancient times, when Latinos used it to treat sore and swollen eyes. This research evaluated the antimicrobial potential, the cytotoxic and genotoxic effects, and some chromatographic profiles of the hydroethanolic extract of leaves, stems and roots of S. praealtum. Methods: The antimicrobial activities were carried out by broth microdilution and agar diffusion. In vitro cytotoxicity was evaluated by cell cultures of Aedes albopictus and the selectivity index (SI) was estimated: SI=CI50/MIC. Genotoxic and systemic toxic effects of S. praealtum leaves were analyzed by micronucleus assay in mice bone marrow. Chromatographic profiles and mass spectra were investigated by GC-MS. Results: Gram-positive (B. subtilis, B. cereus, M. luteus, E. faecalis and S. aureus) and gram-negative (E. coli, E. aerogenes, S. marcescens, P. aeruginosa, P. mirabilis and S. typhimurium) bacteria exhibited MICs ranging from 12.5–50 and 0–50 mg/ ml, respectively. Sedum praealtum showed no efficacy against M. tuberculosis and M. bovis. Cytotoxicity (CI50) of S. praealtum was 4.22 and 5.96 mg/ml for leaves and stems, respectively, while its roots showed no cytotoxicity. Micronucleated polychromatic erythrocytes (MNPCEs) analyzes showed no differences between treatment doses (0.5–2 g/kg) and negative control (NaCl), but the PCE/NCE ratio (polychromatic erythrocyte/normochromatic erythrocyte) showed significant differences. Phytochemical screening identified thirteen compounds in the leaves, stems and roots of S. praealtum potentially associated with their biological activities. Conclusions: This research comprises a first scientific study on genotoxicity, cytotoxicity and antimicrobial effects of S. praealtum (Balsam), and it provides an initial theoretical foundation for its comprehensive use. Results showed antibacterial action of S. praealtum against gram-positive bacteria and some gram-negative species (depending on the plant anatomical part), but ineffective antimycobacterial action. However, S. praealtum leaves and stems display potential cytotoxicity, contributing to the SI < 1 values. In addition, S. praealtum leaves exhibit no clastogenic and/or aneugenic effects, but it has systemic toxicity dose-independent.Laboratory of Microbiology and Immunology Department of Oral Diagnosis Dental School of Piracicaba State University of Campinas (FOP/UNICAMP)Center for Research and Postgraduate Studies in Animal Science Pathology and Animal Pharmacology Area University of Alfenas (UNIFENAS)Laboratory of Pharmacogenetics and Molecular Biology Faculty of Medical Sciences University of Alfenas (UNIFENAS)Department of Exact Sciences College of Agriculture University of São Paulo (ESALQ/USP)Laboratory of Oral Microbiology and Immunology Department of Physiology and Oral Pathology Araraquara School of Dentistry São Paulo State University (FOAr/UNESP)Reproductive Biology Center Federal University of Juiz de Fora (UFJF)Laboratory of Oral Microbiology and Immunology Department of Physiology and Oral Pathology Araraquara School of Dentistry São Paulo State University (FOAr/UNESP)Universidade Estadual de Campinas (UNICAMP)University of Alfenas (UNIFENAS)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Federal University of Juiz de Fora (UFJF)Gomes Boriollo, Marcelo FabianoMarques, Milene BuenoDa Silva, Thaísla AndrielleDa Silva, Jeferson JúniorDias, Reigson AlvesSilva Filho, Thyago Henrique NevesRibeiro Melo, Isadora LetíciaDos Santos Dias, Carlos TadeuDe Carvalho Bernardo, Wagner Luís [UNESP]De Mello Silva Oliveira, NelmaPeters, Vera MariaHöfling, José FranciscoPalomari Spolidorio, Denise Madalena [UNESP]2020-12-12T02:10:22Z2020-12-12T02:10:22Z2020-04-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s12906-020-02915-6BMC Complementary Medicine and Therapies, v. 20, n. 1, 2020.2662-7671http://hdl.handle.net/11449/20058010.1186/s12906-020-02915-62-s2.0-85086083329Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Complementary Medicine and Therapiesinfo:eu-repo/semantics/openAccess2021-10-23T14:48:13Zoai:repositorio.unesp.br:11449/200580Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:20:57.492051Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antimicrobial potential, phytochemical profile, cytotoxic and genotoxic screening of sedum praealtum a. DC. (balsam) |
title |
Antimicrobial potential, phytochemical profile, cytotoxic and genotoxic screening of sedum praealtum a. DC. (balsam) |
spellingShingle |
Antimicrobial potential, phytochemical profile, cytotoxic and genotoxic screening of sedum praealtum a. DC. (balsam) Gomes Boriollo, Marcelo Fabiano Antimicrobial susceptibility Cytotoxicity Genotoxicity Phytochemical screening Sedum praealtum a. DC |
title_short |
Antimicrobial potential, phytochemical profile, cytotoxic and genotoxic screening of sedum praealtum a. DC. (balsam) |
title_full |
Antimicrobial potential, phytochemical profile, cytotoxic and genotoxic screening of sedum praealtum a. DC. (balsam) |
title_fullStr |
Antimicrobial potential, phytochemical profile, cytotoxic and genotoxic screening of sedum praealtum a. DC. (balsam) |
title_full_unstemmed |
Antimicrobial potential, phytochemical profile, cytotoxic and genotoxic screening of sedum praealtum a. DC. (balsam) |
title_sort |
Antimicrobial potential, phytochemical profile, cytotoxic and genotoxic screening of sedum praealtum a. DC. (balsam) |
author |
Gomes Boriollo, Marcelo Fabiano |
author_facet |
Gomes Boriollo, Marcelo Fabiano Marques, Milene Bueno Da Silva, Thaísla Andrielle Da Silva, Jeferson Júnior Dias, Reigson Alves Silva Filho, Thyago Henrique Neves Ribeiro Melo, Isadora Letícia Dos Santos Dias, Carlos Tadeu De Carvalho Bernardo, Wagner Luís [UNESP] De Mello Silva Oliveira, Nelma Peters, Vera Maria Höfling, José Francisco Palomari Spolidorio, Denise Madalena [UNESP] |
author_role |
author |
author2 |
Marques, Milene Bueno Da Silva, Thaísla Andrielle Da Silva, Jeferson Júnior Dias, Reigson Alves Silva Filho, Thyago Henrique Neves Ribeiro Melo, Isadora Letícia Dos Santos Dias, Carlos Tadeu De Carvalho Bernardo, Wagner Luís [UNESP] De Mello Silva Oliveira, Nelma Peters, Vera Maria Höfling, José Francisco Palomari Spolidorio, Denise Madalena [UNESP] |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) University of Alfenas (UNIFENAS) Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Federal University of Juiz de Fora (UFJF) |
dc.contributor.author.fl_str_mv |
Gomes Boriollo, Marcelo Fabiano Marques, Milene Bueno Da Silva, Thaísla Andrielle Da Silva, Jeferson Júnior Dias, Reigson Alves Silva Filho, Thyago Henrique Neves Ribeiro Melo, Isadora Letícia Dos Santos Dias, Carlos Tadeu De Carvalho Bernardo, Wagner Luís [UNESP] De Mello Silva Oliveira, Nelma Peters, Vera Maria Höfling, José Francisco Palomari Spolidorio, Denise Madalena [UNESP] |
dc.subject.por.fl_str_mv |
Antimicrobial susceptibility Cytotoxicity Genotoxicity Phytochemical screening Sedum praealtum a. DC |
topic |
Antimicrobial susceptibility Cytotoxicity Genotoxicity Phytochemical screening Sedum praealtum a. DC |
description |
Background: Sedum praealtum has been used for a long time in traditional medicine as an analgesic and antiinflammatory agent. Its beneficial effects have been known since ancient times, when Latinos used it to treat sore and swollen eyes. This research evaluated the antimicrobial potential, the cytotoxic and genotoxic effects, and some chromatographic profiles of the hydroethanolic extract of leaves, stems and roots of S. praealtum. Methods: The antimicrobial activities were carried out by broth microdilution and agar diffusion. In vitro cytotoxicity was evaluated by cell cultures of Aedes albopictus and the selectivity index (SI) was estimated: SI=CI50/MIC. Genotoxic and systemic toxic effects of S. praealtum leaves were analyzed by micronucleus assay in mice bone marrow. Chromatographic profiles and mass spectra were investigated by GC-MS. Results: Gram-positive (B. subtilis, B. cereus, M. luteus, E. faecalis and S. aureus) and gram-negative (E. coli, E. aerogenes, S. marcescens, P. aeruginosa, P. mirabilis and S. typhimurium) bacteria exhibited MICs ranging from 12.5–50 and 0–50 mg/ ml, respectively. Sedum praealtum showed no efficacy against M. tuberculosis and M. bovis. Cytotoxicity (CI50) of S. praealtum was 4.22 and 5.96 mg/ml for leaves and stems, respectively, while its roots showed no cytotoxicity. Micronucleated polychromatic erythrocytes (MNPCEs) analyzes showed no differences between treatment doses (0.5–2 g/kg) and negative control (NaCl), but the PCE/NCE ratio (polychromatic erythrocyte/normochromatic erythrocyte) showed significant differences. Phytochemical screening identified thirteen compounds in the leaves, stems and roots of S. praealtum potentially associated with their biological activities. Conclusions: This research comprises a first scientific study on genotoxicity, cytotoxicity and antimicrobial effects of S. praealtum (Balsam), and it provides an initial theoretical foundation for its comprehensive use. Results showed antibacterial action of S. praealtum against gram-positive bacteria and some gram-negative species (depending on the plant anatomical part), but ineffective antimycobacterial action. However, S. praealtum leaves and stems display potential cytotoxicity, contributing to the SI < 1 values. In addition, S. praealtum leaves exhibit no clastogenic and/or aneugenic effects, but it has systemic toxicity dose-independent. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:10:22Z 2020-12-12T02:10:22Z 2020-04-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12906-020-02915-6 BMC Complementary Medicine and Therapies, v. 20, n. 1, 2020. 2662-7671 http://hdl.handle.net/11449/200580 10.1186/s12906-020-02915-6 2-s2.0-85086083329 |
url |
http://dx.doi.org/10.1186/s12906-020-02915-6 http://hdl.handle.net/11449/200580 |
identifier_str_mv |
BMC Complementary Medicine and Therapies, v. 20, n. 1, 2020. 2662-7671 10.1186/s12906-020-02915-6 2-s2.0-85086083329 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Complementary Medicine and Therapies |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128500523073536 |