Triploidization in the streaked prochilod Prochilodus lineatus inferred by flow cytometry, blood smears and karyological approaches
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/jai.14025 http://hdl.handle.net/11449/200176 |
Resumo: | The streaked prochilod Prochilodus lineatus is an important fish species from the Neotropical region. In this study, a protocol of triploidization was established by temperature shock. Fertilized eggs were shocked at 2 min post-fertilization at 0, 38, 40 and 42°C. At 0°C, the embryos were maintained for 30 min, while the rest for 2 min. Heat shocked embryos and control (during all experiment) were incubated at 27°C. The ploidy status was confirmed by flow cytometry, erythrocyte diameter, conventional cytogenetics (Giemsa staining), chromosome banding and fluorescence in situ hybridization using 5S and 18S rDNA probes. Heat-shock at 40°C produced 96.7% of triploids and such a procedure did not reduce the fertilization, hatching rate and the percentage of normal embryos. The use of chromosome banding and FISH gave rise to effective procedure to identify triploids. The data obtained here is innovative for the species and bring new information for basic and applied studies. |
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Triploidization in the streaked prochilod Prochilodus lineatus inferred by flow cytometry, blood smears and karyological approachescharacinchromosomefishpolyploidThe streaked prochilod Prochilodus lineatus is an important fish species from the Neotropical region. In this study, a protocol of triploidization was established by temperature shock. Fertilized eggs were shocked at 2 min post-fertilization at 0, 38, 40 and 42°C. At 0°C, the embryos were maintained for 30 min, while the rest for 2 min. Heat shocked embryos and control (during all experiment) were incubated at 27°C. The ploidy status was confirmed by flow cytometry, erythrocyte diameter, conventional cytogenetics (Giemsa staining), chromosome banding and fluorescence in situ hybridization using 5S and 18S rDNA probes. Heat-shock at 40°C produced 96.7% of triploids and such a procedure did not reduce the fertilization, hatching rate and the percentage of normal embryos. The use of chromosome banding and FISH gave rise to effective procedure to identify triploids. The data obtained here is innovative for the species and bring new information for basic and applied studies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Fish Biotechnology National Center for Research and Conservation of Continental Fish Chico Mendes Institute of Biodiversity ConservationDepartment of Veterinary Medicine – FZEA University of São PauloAquaculture Center São Paulo State UniversityFederal Rural University of Rio de JaneiroFaculdade de Ciências Bauru UNESP – Univ. Estadual PaulistaAquaculture Center São Paulo State UniversityFaculdade de Ciências Bauru UNESP – Univ. Estadual PaulistaFAPESP: 2010/17429-1FAPESP: 2011/11664-1Chico Mendes Institute of Biodiversity ConservationUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Federal Rural University of Rio de JaneiroYasui, George ShiguekiNakaghi, Laura Satiko Okada [UNESP]Monzani, Paulo SérgioNascimento, Nivaldo Ferreira do [UNESP]Pereira dos Santos, MatheusGoes, Caio Augusto Gomes [UNESP]Porto-Foresti, Fábio [UNESP]Senhorini, José Augusto2020-12-12T01:59:40Z2020-12-12T01:59:40Z2020-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article339-344http://dx.doi.org/10.1111/jai.14025Journal of Applied Ichthyology, v. 36, n. 3, p. 339-344, 2020.1439-04260175-8659http://hdl.handle.net/11449/20017610.1111/jai.140252-s2.0-85081726801Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Ichthyologyinfo:eu-repo/semantics/openAccess2024-04-09T15:43:25Zoai:repositorio.unesp.br:11449/200176Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:21:30.131550Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Triploidization in the streaked prochilod Prochilodus lineatus inferred by flow cytometry, blood smears and karyological approaches |
title |
Triploidization in the streaked prochilod Prochilodus lineatus inferred by flow cytometry, blood smears and karyological approaches |
spellingShingle |
Triploidization in the streaked prochilod Prochilodus lineatus inferred by flow cytometry, blood smears and karyological approaches Yasui, George Shigueki characin chromosome fish polyploid |
title_short |
Triploidization in the streaked prochilod Prochilodus lineatus inferred by flow cytometry, blood smears and karyological approaches |
title_full |
Triploidization in the streaked prochilod Prochilodus lineatus inferred by flow cytometry, blood smears and karyological approaches |
title_fullStr |
Triploidization in the streaked prochilod Prochilodus lineatus inferred by flow cytometry, blood smears and karyological approaches |
title_full_unstemmed |
Triploidization in the streaked prochilod Prochilodus lineatus inferred by flow cytometry, blood smears and karyological approaches |
title_sort |
Triploidization in the streaked prochilod Prochilodus lineatus inferred by flow cytometry, blood smears and karyological approaches |
author |
Yasui, George Shigueki |
author_facet |
Yasui, George Shigueki Nakaghi, Laura Satiko Okada [UNESP] Monzani, Paulo Sérgio Nascimento, Nivaldo Ferreira do [UNESP] Pereira dos Santos, Matheus Goes, Caio Augusto Gomes [UNESP] Porto-Foresti, Fábio [UNESP] Senhorini, José Augusto |
author_role |
author |
author2 |
Nakaghi, Laura Satiko Okada [UNESP] Monzani, Paulo Sérgio Nascimento, Nivaldo Ferreira do [UNESP] Pereira dos Santos, Matheus Goes, Caio Augusto Gomes [UNESP] Porto-Foresti, Fábio [UNESP] Senhorini, José Augusto |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Chico Mendes Institute of Biodiversity Conservation Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Federal Rural University of Rio de Janeiro |
dc.contributor.author.fl_str_mv |
Yasui, George Shigueki Nakaghi, Laura Satiko Okada [UNESP] Monzani, Paulo Sérgio Nascimento, Nivaldo Ferreira do [UNESP] Pereira dos Santos, Matheus Goes, Caio Augusto Gomes [UNESP] Porto-Foresti, Fábio [UNESP] Senhorini, José Augusto |
dc.subject.por.fl_str_mv |
characin chromosome fish polyploid |
topic |
characin chromosome fish polyploid |
description |
The streaked prochilod Prochilodus lineatus is an important fish species from the Neotropical region. In this study, a protocol of triploidization was established by temperature shock. Fertilized eggs were shocked at 2 min post-fertilization at 0, 38, 40 and 42°C. At 0°C, the embryos were maintained for 30 min, while the rest for 2 min. Heat shocked embryos and control (during all experiment) were incubated at 27°C. The ploidy status was confirmed by flow cytometry, erythrocyte diameter, conventional cytogenetics (Giemsa staining), chromosome banding and fluorescence in situ hybridization using 5S and 18S rDNA probes. Heat-shock at 40°C produced 96.7% of triploids and such a procedure did not reduce the fertilization, hatching rate and the percentage of normal embryos. The use of chromosome banding and FISH gave rise to effective procedure to identify triploids. The data obtained here is innovative for the species and bring new information for basic and applied studies. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:59:40Z 2020-12-12T01:59:40Z 2020-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/jai.14025 Journal of Applied Ichthyology, v. 36, n. 3, p. 339-344, 2020. 1439-0426 0175-8659 http://hdl.handle.net/11449/200176 10.1111/jai.14025 2-s2.0-85081726801 |
url |
http://dx.doi.org/10.1111/jai.14025 http://hdl.handle.net/11449/200176 |
identifier_str_mv |
Journal of Applied Ichthyology, v. 36, n. 3, p. 339-344, 2020. 1439-0426 0175-8659 10.1111/jai.14025 2-s2.0-85081726801 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Ichthyology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
339-344 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129419683823616 |