Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1414-431X20122561 http://hdl.handle.net/11449/28086 |
Resumo: | This study investigated the consequences of intrauterine protein restriction on the gastrointestinal tract and particularly on the gene expression and activity of intestinal disaccharidases in the adult offspring. Wistar rat dams were fed isocaloric diets containing 6% protein (restricted, n = 8) or 17% protein (control, n = 8) throughout gestation. Male offspring (n = 5-8 in each group) were evaluated at 3 or 16 weeks of age. Maternal protein restriction during pregnancy produced offspring with growth restriction from birth (5.7 ± 0.1 vs 6.3 ± 0.1 g; mean ± SE) to weaning (42.4 ± 1.3 vs 49.1 ± 1.6 g), although at 16 weeks of age their body weight was similar to control (421.7 ± 8.9 and 428.5 ± 8.5 g). Maternal protein restriction also increased lactase activity in the proximal (0.23 ± 0.02 vs 0.15 ± 0.02), medial (0.30 ± 0.06 vs 0.14 ± 0.01) and distal (0.43 ± 0.07 vs 0.07 ± 0.02 U·g-1·min-1) small intestine, and mRNA lactase abundance in the proximal intestine (7.96 ± 1.11 vs 2.38 ± 0.47 relative units) of 3-week-old offspring rats. In addition, maternal protein restriction increased sucrase activity (1.20 ± 0.02 vs 0.91 ± 0.02 U·g-1·min-1) and sucrase mRNA abundance (4.48 ± 0.51 vs 1.95 ± 0.17 relative units) in the duodenum of 16-week-old rats. In conclusion, the present study shows for the first time that intrauterine protein restriction affects gene expression of intestinal enzymes in offspring. |
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Repositório Institucional da UNESP |
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Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspringmRNA enzyme abundanceMaternal protein restrictionDisaccharidase activityFetal programmingThis study investigated the consequences of intrauterine protein restriction on the gastrointestinal tract and particularly on the gene expression and activity of intestinal disaccharidases in the adult offspring. Wistar rat dams were fed isocaloric diets containing 6% protein (restricted, n = 8) or 17% protein (control, n = 8) throughout gestation. Male offspring (n = 5-8 in each group) were evaluated at 3 or 16 weeks of age. Maternal protein restriction during pregnancy produced offspring with growth restriction from birth (5.7 ± 0.1 vs 6.3 ± 0.1 g; mean ± SE) to weaning (42.4 ± 1.3 vs 49.1 ± 1.6 g), although at 16 weeks of age their body weight was similar to control (421.7 ± 8.9 and 428.5 ± 8.5 g). Maternal protein restriction also increased lactase activity in the proximal (0.23 ± 0.02 vs 0.15 ± 0.02), medial (0.30 ± 0.06 vs 0.14 ± 0.01) and distal (0.43 ± 0.07 vs 0.07 ± 0.02 U·g-1·min-1) small intestine, and mRNA lactase abundance in the proximal intestine (7.96 ± 1.11 vs 2.38 ± 0.47 relative units) of 3-week-old offspring rats. In addition, maternal protein restriction increased sucrase activity (1.20 ± 0.02 vs 0.91 ± 0.02 U·g-1·min-1) and sucrase mRNA abundance (4.48 ± 0.51 vs 1.95 ± 0.17 relative units) in the duodenum of 16-week-old rats. In conclusion, the present study shows for the first time that intrauterine protein restriction affects gene expression of intestinal enzymes in offspring.Universidade Estadual Paulista Instituto de Biociências Departamento de FisiologiaUniversidade Estadual Paulista Instituto de Biociências Departamento de FisiologiaAssociação Brasileira de Divulgação Científica (ABRADIC)Universidade Estadual Paulista (Unesp)Pinheiro, D.F.Pacheco, P.D.G.Alvarenga, P.V.Buratini Jr, J.Castilho, A.C.S.Lima, P.F.Sartori, D.R.S.Vicentini-Paulino, M.L.M.2014-05-20T15:11:34Z2014-05-20T15:11:34Z2013-03-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article287-292application/pdfhttp://dx.doi.org/10.1590/1414-431X20122561Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 46, n. 3, p. 287-292, 2013.0100-879Xhttp://hdl.handle.net/11449/2808610.1590/1414-431X20122561S0100-879X2013000300287WOS:000320004000011S0100-879X2013000300287.pdf5272388662903892SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Medical and Biological Research1.492info:eu-repo/semantics/openAccess2023-11-22T06:15:56Zoai:repositorio.unesp.br:11449/28086Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-22T06:15:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring |
title |
Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring |
spellingShingle |
Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring Pinheiro, D.F. mRNA enzyme abundance Maternal protein restriction Disaccharidase activity Fetal programming |
title_short |
Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring |
title_full |
Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring |
title_fullStr |
Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring |
title_full_unstemmed |
Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring |
title_sort |
Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring |
author |
Pinheiro, D.F. |
author_facet |
Pinheiro, D.F. Pacheco, P.D.G. Alvarenga, P.V. Buratini Jr, J. Castilho, A.C.S. Lima, P.F. Sartori, D.R.S. Vicentini-Paulino, M.L.M. |
author_role |
author |
author2 |
Pacheco, P.D.G. Alvarenga, P.V. Buratini Jr, J. Castilho, A.C.S. Lima, P.F. Sartori, D.R.S. Vicentini-Paulino, M.L.M. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pinheiro, D.F. Pacheco, P.D.G. Alvarenga, P.V. Buratini Jr, J. Castilho, A.C.S. Lima, P.F. Sartori, D.R.S. Vicentini-Paulino, M.L.M. |
dc.subject.por.fl_str_mv |
mRNA enzyme abundance Maternal protein restriction Disaccharidase activity Fetal programming |
topic |
mRNA enzyme abundance Maternal protein restriction Disaccharidase activity Fetal programming |
description |
This study investigated the consequences of intrauterine protein restriction on the gastrointestinal tract and particularly on the gene expression and activity of intestinal disaccharidases in the adult offspring. Wistar rat dams were fed isocaloric diets containing 6% protein (restricted, n = 8) or 17% protein (control, n = 8) throughout gestation. Male offspring (n = 5-8 in each group) were evaluated at 3 or 16 weeks of age. Maternal protein restriction during pregnancy produced offspring with growth restriction from birth (5.7 ± 0.1 vs 6.3 ± 0.1 g; mean ± SE) to weaning (42.4 ± 1.3 vs 49.1 ± 1.6 g), although at 16 weeks of age their body weight was similar to control (421.7 ± 8.9 and 428.5 ± 8.5 g). Maternal protein restriction also increased lactase activity in the proximal (0.23 ± 0.02 vs 0.15 ± 0.02), medial (0.30 ± 0.06 vs 0.14 ± 0.01) and distal (0.43 ± 0.07 vs 0.07 ± 0.02 U·g-1·min-1) small intestine, and mRNA lactase abundance in the proximal intestine (7.96 ± 1.11 vs 2.38 ± 0.47 relative units) of 3-week-old offspring rats. In addition, maternal protein restriction increased sucrase activity (1.20 ± 0.02 vs 0.91 ± 0.02 U·g-1·min-1) and sucrase mRNA abundance (4.48 ± 0.51 vs 1.95 ± 0.17 relative units) in the duodenum of 16-week-old rats. In conclusion, the present study shows for the first time that intrauterine protein restriction affects gene expression of intestinal enzymes in offspring. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-15 2014-05-20T15:11:34Z 2014-05-20T15:11:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1414-431X20122561 Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 46, n. 3, p. 287-292, 2013. 0100-879X http://hdl.handle.net/11449/28086 10.1590/1414-431X20122561 S0100-879X2013000300287 WOS:000320004000011 S0100-879X2013000300287.pdf 5272388662903892 |
url |
http://dx.doi.org/10.1590/1414-431X20122561 http://hdl.handle.net/11449/28086 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 46, n. 3, p. 287-292, 2013. 0100-879X 10.1590/1414-431X20122561 S0100-879X2013000300287 WOS:000320004000011 S0100-879X2013000300287.pdf 5272388662903892 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research 1.492 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
287-292 application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica (ABRADIC) |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica (ABRADIC) |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046607786082304 |