Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.fsi.2022.09.030 http://hdl.handle.net/11449/247654 |
Resumo: | To identify activation pathways and effector mechanisms of innate immunity in fish has become relevant for the sanitary management of intensive fish farming. However, little is known about the blocking of cysteinyl leukotrienes receptors (CysLTRs) and their effects in teleost fish. Our study evaluated the anti-inflammatory effect of 250 and 500 μg zafirlukast (antagonist of CysLTRs)/kg b.w., administered orally in the diet, during acute inflammatory reaction induced by Aeromonas hydrophila bacterins in Oreochromis niloticus. 80 tilapia were distributed in 10 aquariums (100L of water each, n = 8) to constitute three treatments: Control (inoculated with A. hydrophila bacterin and untreated); Treated with 250 μg or 500 μg of zafirlukast/kg b.w. and inoculated. To be evaluated in three periods: 6, 24 and 48 h post-inoculation (HPI), totaling nine aquariums. A tenth group was sampled without any stimulus to constitute reference values (Physiological standards). Tilapia treated with zafirlukast demonstrated dose-response effect in the decrease of accumulated inflammatory cells, strongly influenced by granulocytes and macrophages. Zafirlukast treated-tilapia showed decrease in blood leukocyte counts (mainly neutrophils, and monocytes) and reactive oxygen species production. Treatment with zafirlukast resulted in down-regulation of ceruloplasmin, complement 3, alpha2-macroglobulin, transferrin and apolipoprotein A1, as well as up-regulation of haptoglobin. Our study provided convincing results in the pathophysiology of tilapia inflammatory reaction, considering that treatment with zafirlukast, antagonist of cysteinyl leukotriene receptors, resulted in a dose-response effect by suppressing the dynamics between leukocytes in the bloodstream and cell accumulation in the inflamed focus, as well as modulated the leukocyte oxidative burst and the acute phase protein response. |
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Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticusCichlidsCysteine leukotrienesInnate immunityLipoxygenaseMacrophagesNeutrophilsTo identify activation pathways and effector mechanisms of innate immunity in fish has become relevant for the sanitary management of intensive fish farming. However, little is known about the blocking of cysteinyl leukotrienes receptors (CysLTRs) and their effects in teleost fish. Our study evaluated the anti-inflammatory effect of 250 and 500 μg zafirlukast (antagonist of CysLTRs)/kg b.w., administered orally in the diet, during acute inflammatory reaction induced by Aeromonas hydrophila bacterins in Oreochromis niloticus. 80 tilapia were distributed in 10 aquariums (100L of water each, n = 8) to constitute three treatments: Control (inoculated with A. hydrophila bacterin and untreated); Treated with 250 μg or 500 μg of zafirlukast/kg b.w. and inoculated. To be evaluated in three periods: 6, 24 and 48 h post-inoculation (HPI), totaling nine aquariums. A tenth group was sampled without any stimulus to constitute reference values (Physiological standards). Tilapia treated with zafirlukast demonstrated dose-response effect in the decrease of accumulated inflammatory cells, strongly influenced by granulocytes and macrophages. Zafirlukast treated-tilapia showed decrease in blood leukocyte counts (mainly neutrophils, and monocytes) and reactive oxygen species production. Treatment with zafirlukast resulted in down-regulation of ceruloplasmin, complement 3, alpha2-macroglobulin, transferrin and apolipoprotein A1, as well as up-regulation of haptoglobin. Our study provided convincing results in the pathophysiology of tilapia inflammatory reaction, considering that treatment with zafirlukast, antagonist of cysteinyl leukotriene receptors, resulted in a dose-response effect by suppressing the dynamics between leukocytes in the bloodstream and cell accumulation in the inflamed focus, as well as modulated the leukocyte oxidative burst and the acute phase protein response.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Preventive Veterinary Medicine São Paulo State University (UNESP), SPDepartment of Veterinary Medicine Federal University of Rondônia (UNIR), ROAquaculture Center São Paulo State University (CAUNESP), SPDeparment of Pharmacology University of São Paulo-ICB/USP, SPLaboratory of Animal Pharmacology and Toxicology Brazil University – (UB), SPDepartment of Preventive Veterinary Medicine São Paulo State University (UNESP), SPAquaculture Center São Paulo State University (CAUNESP), SPFAPESP: 2017/03094-7Universidade Estadual Paulista (UNESP)Federal University of Rondônia (UNIR)Universidade de São Paulo (USP)Brazil University – (UB)Aracati, Mayumi Fernanda [UNESP]Luporini de Oliveira, Susana [UNESP]Rodrigues, Leticia Franchin [UNESP]Carlino da Costa, Camila [UNESP]Cristina de Moraes, Alessandrada Rosa Prado, Ed Johnny [UNESP]Fernandes, Dayanne Carla [UNESP]Vaz Farias, Thais Heloisa [UNESP]Eto, Silas Fernandes [UNESP]Charlie-Silva, Ivesde Andrade Belo, Marco Antonio [UNESP]2023-07-29T13:22:11Z2023-07-29T13:22:11Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article323-331http://dx.doi.org/10.1016/j.fsi.2022.09.030Fish and Shellfish Immunology, v. 130, p. 323-331.1095-99471050-4648http://hdl.handle.net/11449/24765410.1016/j.fsi.2022.09.0302-s2.0-85138558035Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish and Shellfish Immunologyinfo:eu-repo/semantics/openAccess2024-04-09T15:36:43Zoai:repositorio.unesp.br:11449/247654Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:36:10.999315Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus |
title |
Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus |
spellingShingle |
Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus Aracati, Mayumi Fernanda [UNESP] Cichlids Cysteine leukotrienes Innate immunity Lipoxygenase Macrophages Neutrophils |
title_short |
Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus |
title_full |
Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus |
title_fullStr |
Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus |
title_full_unstemmed |
Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus |
title_sort |
Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus |
author |
Aracati, Mayumi Fernanda [UNESP] |
author_facet |
Aracati, Mayumi Fernanda [UNESP] Luporini de Oliveira, Susana [UNESP] Rodrigues, Leticia Franchin [UNESP] Carlino da Costa, Camila [UNESP] Cristina de Moraes, Alessandra da Rosa Prado, Ed Johnny [UNESP] Fernandes, Dayanne Carla [UNESP] Vaz Farias, Thais Heloisa [UNESP] Eto, Silas Fernandes [UNESP] Charlie-Silva, Ives de Andrade Belo, Marco Antonio [UNESP] |
author_role |
author |
author2 |
Luporini de Oliveira, Susana [UNESP] Rodrigues, Leticia Franchin [UNESP] Carlino da Costa, Camila [UNESP] Cristina de Moraes, Alessandra da Rosa Prado, Ed Johnny [UNESP] Fernandes, Dayanne Carla [UNESP] Vaz Farias, Thais Heloisa [UNESP] Eto, Silas Fernandes [UNESP] Charlie-Silva, Ives de Andrade Belo, Marco Antonio [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Federal University of Rondônia (UNIR) Universidade de São Paulo (USP) Brazil University – (UB) |
dc.contributor.author.fl_str_mv |
Aracati, Mayumi Fernanda [UNESP] Luporini de Oliveira, Susana [UNESP] Rodrigues, Leticia Franchin [UNESP] Carlino da Costa, Camila [UNESP] Cristina de Moraes, Alessandra da Rosa Prado, Ed Johnny [UNESP] Fernandes, Dayanne Carla [UNESP] Vaz Farias, Thais Heloisa [UNESP] Eto, Silas Fernandes [UNESP] Charlie-Silva, Ives de Andrade Belo, Marco Antonio [UNESP] |
dc.subject.por.fl_str_mv |
Cichlids Cysteine leukotrienes Innate immunity Lipoxygenase Macrophages Neutrophils |
topic |
Cichlids Cysteine leukotrienes Innate immunity Lipoxygenase Macrophages Neutrophils |
description |
To identify activation pathways and effector mechanisms of innate immunity in fish has become relevant for the sanitary management of intensive fish farming. However, little is known about the blocking of cysteinyl leukotrienes receptors (CysLTRs) and their effects in teleost fish. Our study evaluated the anti-inflammatory effect of 250 and 500 μg zafirlukast (antagonist of CysLTRs)/kg b.w., administered orally in the diet, during acute inflammatory reaction induced by Aeromonas hydrophila bacterins in Oreochromis niloticus. 80 tilapia were distributed in 10 aquariums (100L of water each, n = 8) to constitute three treatments: Control (inoculated with A. hydrophila bacterin and untreated); Treated with 250 μg or 500 μg of zafirlukast/kg b.w. and inoculated. To be evaluated in three periods: 6, 24 and 48 h post-inoculation (HPI), totaling nine aquariums. A tenth group was sampled without any stimulus to constitute reference values (Physiological standards). Tilapia treated with zafirlukast demonstrated dose-response effect in the decrease of accumulated inflammatory cells, strongly influenced by granulocytes and macrophages. Zafirlukast treated-tilapia showed decrease in blood leukocyte counts (mainly neutrophils, and monocytes) and reactive oxygen species production. Treatment with zafirlukast resulted in down-regulation of ceruloplasmin, complement 3, alpha2-macroglobulin, transferrin and apolipoprotein A1, as well as up-regulation of haptoglobin. Our study provided convincing results in the pathophysiology of tilapia inflammatory reaction, considering that treatment with zafirlukast, antagonist of cysteinyl leukotriene receptors, resulted in a dose-response effect by suppressing the dynamics between leukocytes in the bloodstream and cell accumulation in the inflamed focus, as well as modulated the leukocyte oxidative burst and the acute phase protein response. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-01 2023-07-29T13:22:11Z 2023-07-29T13:22:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.fsi.2022.09.030 Fish and Shellfish Immunology, v. 130, p. 323-331. 1095-9947 1050-4648 http://hdl.handle.net/11449/247654 10.1016/j.fsi.2022.09.030 2-s2.0-85138558035 |
url |
http://dx.doi.org/10.1016/j.fsi.2022.09.030 http://hdl.handle.net/11449/247654 |
identifier_str_mv |
Fish and Shellfish Immunology, v. 130, p. 323-331. 1095-9947 1050-4648 10.1016/j.fsi.2022.09.030 2-s2.0-85138558035 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fish and Shellfish Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
323-331 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129095179960320 |