Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus

Detalhes bibliográficos
Autor(a) principal: Aracati, Mayumi Fernanda [UNESP]
Data de Publicação: 2022
Outros Autores: Luporini de Oliveira, Susana [UNESP], Rodrigues, Leticia Franchin [UNESP], Carlino da Costa, Camila [UNESP], Cristina de Moraes, Alessandra, da Rosa Prado, Ed Johnny [UNESP], Fernandes, Dayanne Carla [UNESP], Vaz Farias, Thais Heloisa [UNESP], Eto, Silas Fernandes [UNESP], Charlie-Silva, Ives, de Andrade Belo, Marco Antonio [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.fsi.2022.09.030
http://hdl.handle.net/11449/247654
Resumo: To identify activation pathways and effector mechanisms of innate immunity in fish has become relevant for the sanitary management of intensive fish farming. However, little is known about the blocking of cysteinyl leukotrienes receptors (CysLTRs) and their effects in teleost fish. Our study evaluated the anti-inflammatory effect of 250 and 500 μg zafirlukast (antagonist of CysLTRs)/kg b.w., administered orally in the diet, during acute inflammatory reaction induced by Aeromonas hydrophila bacterins in Oreochromis niloticus. 80 tilapia were distributed in 10 aquariums (100L of water each, n = 8) to constitute three treatments: Control (inoculated with A. hydrophila bacterin and untreated); Treated with 250 μg or 500 μg of zafirlukast/kg b.w. and inoculated. To be evaluated in three periods: 6, 24 and 48 h post-inoculation (HPI), totaling nine aquariums. A tenth group was sampled without any stimulus to constitute reference values (Physiological standards). Tilapia treated with zafirlukast demonstrated dose-response effect in the decrease of accumulated inflammatory cells, strongly influenced by granulocytes and macrophages. Zafirlukast treated-tilapia showed decrease in blood leukocyte counts (mainly neutrophils, and monocytes) and reactive oxygen species production. Treatment with zafirlukast resulted in down-regulation of ceruloplasmin, complement 3, alpha2-macroglobulin, transferrin and apolipoprotein A1, as well as up-regulation of haptoglobin. Our study provided convincing results in the pathophysiology of tilapia inflammatory reaction, considering that treatment with zafirlukast, antagonist of cysteinyl leukotriene receptors, resulted in a dose-response effect by suppressing the dynamics between leukocytes in the bloodstream and cell accumulation in the inflamed focus, as well as modulated the leukocyte oxidative burst and the acute phase protein response.
id UNSP_9e77930e9947b91efe82d75e37b360cb
oai_identifier_str oai:repositorio.unesp.br:11449/247654
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticusCichlidsCysteine leukotrienesInnate immunityLipoxygenaseMacrophagesNeutrophilsTo identify activation pathways and effector mechanisms of innate immunity in fish has become relevant for the sanitary management of intensive fish farming. However, little is known about the blocking of cysteinyl leukotrienes receptors (CysLTRs) and their effects in teleost fish. Our study evaluated the anti-inflammatory effect of 250 and 500 μg zafirlukast (antagonist of CysLTRs)/kg b.w., administered orally in the diet, during acute inflammatory reaction induced by Aeromonas hydrophila bacterins in Oreochromis niloticus. 80 tilapia were distributed in 10 aquariums (100L of water each, n = 8) to constitute three treatments: Control (inoculated with A. hydrophila bacterin and untreated); Treated with 250 μg or 500 μg of zafirlukast/kg b.w. and inoculated. To be evaluated in three periods: 6, 24 and 48 h post-inoculation (HPI), totaling nine aquariums. A tenth group was sampled without any stimulus to constitute reference values (Physiological standards). Tilapia treated with zafirlukast demonstrated dose-response effect in the decrease of accumulated inflammatory cells, strongly influenced by granulocytes and macrophages. Zafirlukast treated-tilapia showed decrease in blood leukocyte counts (mainly neutrophils, and monocytes) and reactive oxygen species production. Treatment with zafirlukast resulted in down-regulation of ceruloplasmin, complement 3, alpha2-macroglobulin, transferrin and apolipoprotein A1, as well as up-regulation of haptoglobin. Our study provided convincing results in the pathophysiology of tilapia inflammatory reaction, considering that treatment with zafirlukast, antagonist of cysteinyl leukotriene receptors, resulted in a dose-response effect by suppressing the dynamics between leukocytes in the bloodstream and cell accumulation in the inflamed focus, as well as modulated the leukocyte oxidative burst and the acute phase protein response.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Preventive Veterinary Medicine São Paulo State University (UNESP), SPDepartment of Veterinary Medicine Federal University of Rondônia (UNIR), ROAquaculture Center São Paulo State University (CAUNESP), SPDeparment of Pharmacology University of São Paulo-ICB/USP, SPLaboratory of Animal Pharmacology and Toxicology Brazil University – (UB), SPDepartment of Preventive Veterinary Medicine São Paulo State University (UNESP), SPAquaculture Center São Paulo State University (CAUNESP), SPFAPESP: 2017/03094-7Universidade Estadual Paulista (UNESP)Federal University of Rondônia (UNIR)Universidade de São Paulo (USP)Brazil University – (UB)Aracati, Mayumi Fernanda [UNESP]Luporini de Oliveira, Susana [UNESP]Rodrigues, Leticia Franchin [UNESP]Carlino da Costa, Camila [UNESP]Cristina de Moraes, Alessandrada Rosa Prado, Ed Johnny [UNESP]Fernandes, Dayanne Carla [UNESP]Vaz Farias, Thais Heloisa [UNESP]Eto, Silas Fernandes [UNESP]Charlie-Silva, Ivesde Andrade Belo, Marco Antonio [UNESP]2023-07-29T13:22:11Z2023-07-29T13:22:11Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article323-331http://dx.doi.org/10.1016/j.fsi.2022.09.030Fish and Shellfish Immunology, v. 130, p. 323-331.1095-99471050-4648http://hdl.handle.net/11449/24765410.1016/j.fsi.2022.09.0302-s2.0-85138558035Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish and Shellfish Immunologyinfo:eu-repo/semantics/openAccess2024-04-09T15:36:43Zoai:repositorio.unesp.br:11449/247654Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:36:10.999315Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus
title Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus
spellingShingle Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus
Aracati, Mayumi Fernanda [UNESP]
Cichlids
Cysteine leukotrienes
Innate immunity
Lipoxygenase
Macrophages
Neutrophils
title_short Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus
title_full Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus
title_fullStr Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus
title_full_unstemmed Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus
title_sort Antagonism of cysteinyl leukotriene receptors by zafirlukast modulated acute inflammatory reaction in tilapia, Oreochromis niloticus
author Aracati, Mayumi Fernanda [UNESP]
author_facet Aracati, Mayumi Fernanda [UNESP]
Luporini de Oliveira, Susana [UNESP]
Rodrigues, Leticia Franchin [UNESP]
Carlino da Costa, Camila [UNESP]
Cristina de Moraes, Alessandra
da Rosa Prado, Ed Johnny [UNESP]
Fernandes, Dayanne Carla [UNESP]
Vaz Farias, Thais Heloisa [UNESP]
Eto, Silas Fernandes [UNESP]
Charlie-Silva, Ives
de Andrade Belo, Marco Antonio [UNESP]
author_role author
author2 Luporini de Oliveira, Susana [UNESP]
Rodrigues, Leticia Franchin [UNESP]
Carlino da Costa, Camila [UNESP]
Cristina de Moraes, Alessandra
da Rosa Prado, Ed Johnny [UNESP]
Fernandes, Dayanne Carla [UNESP]
Vaz Farias, Thais Heloisa [UNESP]
Eto, Silas Fernandes [UNESP]
Charlie-Silva, Ives
de Andrade Belo, Marco Antonio [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Federal University of Rondônia (UNIR)
Universidade de São Paulo (USP)
Brazil University – (UB)
dc.contributor.author.fl_str_mv Aracati, Mayumi Fernanda [UNESP]
Luporini de Oliveira, Susana [UNESP]
Rodrigues, Leticia Franchin [UNESP]
Carlino da Costa, Camila [UNESP]
Cristina de Moraes, Alessandra
da Rosa Prado, Ed Johnny [UNESP]
Fernandes, Dayanne Carla [UNESP]
Vaz Farias, Thais Heloisa [UNESP]
Eto, Silas Fernandes [UNESP]
Charlie-Silva, Ives
de Andrade Belo, Marco Antonio [UNESP]
dc.subject.por.fl_str_mv Cichlids
Cysteine leukotrienes
Innate immunity
Lipoxygenase
Macrophages
Neutrophils
topic Cichlids
Cysteine leukotrienes
Innate immunity
Lipoxygenase
Macrophages
Neutrophils
description To identify activation pathways and effector mechanisms of innate immunity in fish has become relevant for the sanitary management of intensive fish farming. However, little is known about the blocking of cysteinyl leukotrienes receptors (CysLTRs) and their effects in teleost fish. Our study evaluated the anti-inflammatory effect of 250 and 500 μg zafirlukast (antagonist of CysLTRs)/kg b.w., administered orally in the diet, during acute inflammatory reaction induced by Aeromonas hydrophila bacterins in Oreochromis niloticus. 80 tilapia were distributed in 10 aquariums (100L of water each, n = 8) to constitute three treatments: Control (inoculated with A. hydrophila bacterin and untreated); Treated with 250 μg or 500 μg of zafirlukast/kg b.w. and inoculated. To be evaluated in three periods: 6, 24 and 48 h post-inoculation (HPI), totaling nine aquariums. A tenth group was sampled without any stimulus to constitute reference values (Physiological standards). Tilapia treated with zafirlukast demonstrated dose-response effect in the decrease of accumulated inflammatory cells, strongly influenced by granulocytes and macrophages. Zafirlukast treated-tilapia showed decrease in blood leukocyte counts (mainly neutrophils, and monocytes) and reactive oxygen species production. Treatment with zafirlukast resulted in down-regulation of ceruloplasmin, complement 3, alpha2-macroglobulin, transferrin and apolipoprotein A1, as well as up-regulation of haptoglobin. Our study provided convincing results in the pathophysiology of tilapia inflammatory reaction, considering that treatment with zafirlukast, antagonist of cysteinyl leukotriene receptors, resulted in a dose-response effect by suppressing the dynamics between leukocytes in the bloodstream and cell accumulation in the inflamed focus, as well as modulated the leukocyte oxidative burst and the acute phase protein response.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-01
2023-07-29T13:22:11Z
2023-07-29T13:22:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.fsi.2022.09.030
Fish and Shellfish Immunology, v. 130, p. 323-331.
1095-9947
1050-4648
http://hdl.handle.net/11449/247654
10.1016/j.fsi.2022.09.030
2-s2.0-85138558035
url http://dx.doi.org/10.1016/j.fsi.2022.09.030
http://hdl.handle.net/11449/247654
identifier_str_mv Fish and Shellfish Immunology, v. 130, p. 323-331.
1095-9947
1050-4648
10.1016/j.fsi.2022.09.030
2-s2.0-85138558035
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fish and Shellfish Immunology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 323-331
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129095179960320