Immobilization of a Plant Lipase from Pachira aquatica in Alginate and Alginate/PVA Beads

Detalhes bibliográficos
Autor(a) principal: Bonine, Barbara Martineli [UNESP]
Data de Publicação: 2014
Outros Autores: Polizelli, Patricia Peres, Bonilla-Rodriguez, Gustavo Orlando [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.hindawi.com/journals/er/2014/738739/
http://hdl.handle.net/11449/122576
Resumo: This study reports the immobilization of a new lipase isolated from oleaginous seeds of Pachira aquatica, using beads of calcium alginate (Alg) and poly(vinyl alcohol) (PVA). We evaluated the morphology, number of cycles of reuse, optimum temperature, and temperature stability of both immobilization methods compared to the free enzyme. The immobilized enzymes were more stable than the free enzyme, keeping 60% of the original activity after 4 h at 50°C. The immobilized lipase was reused several times, with activity decreasing to approximately 50% after 5 cycles. Both the free and immobilized enzymes were found to be optimally active between 30 and 40°C.
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spelling Immobilization of a Plant Lipase from Pachira aquatica in Alginate and Alginate/PVA BeadsThis study reports the immobilization of a new lipase isolated from oleaginous seeds of Pachira aquatica, using beads of calcium alginate (Alg) and poly(vinyl alcohol) (PVA). We evaluated the morphology, number of cycles of reuse, optimum temperature, and temperature stability of both immobilization methods compared to the free enzyme. The immobilized enzymes were more stable than the free enzyme, keeping 60% of the original activity after 4 h at 50°C. The immobilized lipase was reused several times, with activity decreasing to approximately 50% after 5 cycles. Both the free and immobilized enzymes were found to be optimally active between 30 and 40°C.Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Ciências Ambientais, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, São José do Rio Preto, Rua Cristovão Colombo 2265, Jd. Nazaré, CEP 15054000, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Ciências Ambientais, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, São José do Rio Preto, Rua Cristovão Colombo 2265, Jd. Nazaré, CEP 15054000, SP, BrasilDepartment of Pharmacy and Biochemistry, Centro Universitario de Rio Preto, UNIRP, Rua Ivete Gabriel Atique 45, 15025-400 Sao Jos ˜ e do Rio Preto, SP, BrazilUniversidade Estadual Paulista (Unesp)Bonine, Barbara Martineli [UNESP]Polizelli, Patricia PeresBonilla-Rodriguez, Gustavo Orlando [UNESP]2015-04-27T11:55:52Z2015-04-27T11:55:52Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-7application/pdfhttp://www.hindawi.com/journals/er/2014/738739/Enzyme Research, v. 2014, p. 1-7, 2014.2090-0406http://hdl.handle.net/11449/12257610.1155/2014/738739ISSN2090-0406-2014-2014-01-07.pdf6955258588672130Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnzyme Research0,653info:eu-repo/semantics/openAccess2023-11-02T06:08:24Zoai:repositorio.unesp.br:11449/122576Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:42:49.162376Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Immobilization of a Plant Lipase from Pachira aquatica in Alginate and Alginate/PVA Beads
title Immobilization of a Plant Lipase from Pachira aquatica in Alginate and Alginate/PVA Beads
spellingShingle Immobilization of a Plant Lipase from Pachira aquatica in Alginate and Alginate/PVA Beads
Bonine, Barbara Martineli [UNESP]
title_short Immobilization of a Plant Lipase from Pachira aquatica in Alginate and Alginate/PVA Beads
title_full Immobilization of a Plant Lipase from Pachira aquatica in Alginate and Alginate/PVA Beads
title_fullStr Immobilization of a Plant Lipase from Pachira aquatica in Alginate and Alginate/PVA Beads
title_full_unstemmed Immobilization of a Plant Lipase from Pachira aquatica in Alginate and Alginate/PVA Beads
title_sort Immobilization of a Plant Lipase from Pachira aquatica in Alginate and Alginate/PVA Beads
author Bonine, Barbara Martineli [UNESP]
author_facet Bonine, Barbara Martineli [UNESP]
Polizelli, Patricia Peres
Bonilla-Rodriguez, Gustavo Orlando [UNESP]
author_role author
author2 Polizelli, Patricia Peres
Bonilla-Rodriguez, Gustavo Orlando [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Bonine, Barbara Martineli [UNESP]
Polizelli, Patricia Peres
Bonilla-Rodriguez, Gustavo Orlando [UNESP]
description This study reports the immobilization of a new lipase isolated from oleaginous seeds of Pachira aquatica, using beads of calcium alginate (Alg) and poly(vinyl alcohol) (PVA). We evaluated the morphology, number of cycles of reuse, optimum temperature, and temperature stability of both immobilization methods compared to the free enzyme. The immobilized enzymes were more stable than the free enzyme, keeping 60% of the original activity after 4 h at 50°C. The immobilized lipase was reused several times, with activity decreasing to approximately 50% after 5 cycles. Both the free and immobilized enzymes were found to be optimally active between 30 and 40°C.
publishDate 2014
dc.date.none.fl_str_mv 2014
2015-04-27T11:55:52Z
2015-04-27T11:55:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.hindawi.com/journals/er/2014/738739/
Enzyme Research, v. 2014, p. 1-7, 2014.
2090-0406
http://hdl.handle.net/11449/122576
10.1155/2014/738739
ISSN2090-0406-2014-2014-01-07.pdf
6955258588672130
url http://www.hindawi.com/journals/er/2014/738739/
http://hdl.handle.net/11449/122576
identifier_str_mv Enzyme Research, v. 2014, p. 1-7, 2014.
2090-0406
10.1155/2014/738739
ISSN2090-0406-2014-2014-01-07.pdf
6955258588672130
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Enzyme Research
0,653
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-7
application/pdf
dc.source.none.fl_str_mv Currículo Lattes
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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