Is hypoxia a stressor to American bullfrog tadpoles?

Detalhes bibliográficos
Autor(a) principal: Teixeira, Patricia C. [UNESP]
Data de Publicação: 2014
Outros Autores: França, Fernanda M., Rocha, Guilherme C. Da, Antonucci, Antônio M., Ferreira, Claudia M., Ranzani-paiva, Maria José T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-736X2014000400012
http://hdl.handle.net/11449/110089
Resumo: The aim of this study was to evaluate alterations to the physiological profile of cortisol in pro-metamorphose phase tadpoles of Lithobates catesbeianus exposed to hypoxia stressor in a capture experiment and in a crowding experiment. The capture study was performed by the treatments: stress due to individual capture with a hand net, stress due to batch capture with a hand net and stress due to capture by emptying. Three simultaneous replicates was done witch 12 animals were sampled (6 normoxia - immediately blood collection) and 6 hypoxia - blood collection after 15 min of air exposition) in two collection times with 5 days by intervals. The crowding study was performed by the treatments 1 tadpole L-1, 5 tadpoles L-1 and 10 tadpoles L-1. Three simultaneous replicates was done witch 8 animals (4 normoxia and 4 hypoxia) were sampled in the zero moment (ZM) - blood collection before the experiment, 6 animals/treatment (3 normoxia and 3 hypoxia) to 4 and 8 days and 18 animals/treatment (9 normoxia and 9 hypoxia) to 12 days. The average values to plasmatic cortisol varying from 1.7 to 5.1ng mL-1 (capture study) and 1.0 to 4.2ng mL-1 (crowding study). It concludes that the biomarker tested (cortisol) showed no alterations front of the stressor used. Alternatively, a larger response pattern to these stimuli may have been expressed in another level of an unmeasured hormone (corticosterone). And the bullfrog has great ability to adapt to different management compared to other aquatic organisms, which demonstrates the plasticity of these animals.
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spelling Is hypoxia a stressor to American bullfrog tadpoles?A hipóxia é um estressor para girinos de rã-touro?RaniculturaAmerican bullfrogLithobates catesbeianustadpoleestressecortisolFrogcultureAmerican bullfrogLithobates catesbeianustadpolestresscortisolThe aim of this study was to evaluate alterations to the physiological profile of cortisol in pro-metamorphose phase tadpoles of Lithobates catesbeianus exposed to hypoxia stressor in a capture experiment and in a crowding experiment. The capture study was performed by the treatments: stress due to individual capture with a hand net, stress due to batch capture with a hand net and stress due to capture by emptying. Three simultaneous replicates was done witch 12 animals were sampled (6 normoxia - immediately blood collection) and 6 hypoxia - blood collection after 15 min of air exposition) in two collection times with 5 days by intervals. The crowding study was performed by the treatments 1 tadpole L-1, 5 tadpoles L-1 and 10 tadpoles L-1. Three simultaneous replicates was done witch 8 animals (4 normoxia and 4 hypoxia) were sampled in the zero moment (ZM) - blood collection before the experiment, 6 animals/treatment (3 normoxia and 3 hypoxia) to 4 and 8 days and 18 animals/treatment (9 normoxia and 9 hypoxia) to 12 days. The average values to plasmatic cortisol varying from 1.7 to 5.1ng mL-1 (capture study) and 1.0 to 4.2ng mL-1 (crowding study). It concludes that the biomarker tested (cortisol) showed no alterations front of the stressor used. Alternatively, a larger response pattern to these stimuli may have been expressed in another level of an unmeasured hormone (corticosterone). And the bullfrog has great ability to adapt to different management compared to other aquatic organisms, which demonstrates the plasticity of these animals.Avaliou-se o cortisol em girinos de rã-touro (Lithobates catesbeianus), no estágio de pró-metamorfose ao mecanismo estressor de hipóxia juntamente com captura (1º experimento) e adensamento (2º experimento). O experimento de captura foi composto pelos tratamentos: Captura individual com puçá, Captura em massa com puçá e Captura por escoamento, com 3 réplicas simultâneas onde 12 organismos foram amostrados (6 normoxia - coleta imediata de sangue e 6 hipoxia - coleta de sangue após 15 min de exposição ao ar) em 2 tempos de coleta com intervalo de 5 dias. O experimento de densidade foi composto pelos tratamentos: 1 girino L-1, 5 girinos L-1 e 10 girinos L-1, com 3 réplicas simultâneas onde 8 animais (4 normoxia e 4 hipoxia) foram amostrados no momento zero (MZ) - coleta de sangue anterior ao experimento, 6 animais/tratamento (3 normoxia e 3 hipoxia) para 4 e 8 dias e 18 animais/tratamento (9 normoxia e 9 hipoxia) para 12 dias. Os valores médios para o cortisol plasmático foram de 1,7 a 5,1ng mL-1 (Experimento de Captura); e 1,0 a 4,2ng mL-1 (Experimento de Densidade). Conclui-se que o marcador biologico de estresse utilizado (cortisol) não foi alterado pelo agente extressor. Alternativamente a resposta a este estímulo pode ser expressa em outro nível hormonal (corticosterona). E a rã-touro apresenta ótima capacidade de se adaptar aos diferentes manejos se comparados a outros organismos aquáticos, o que demonstra a plasticidade destes animais.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Centro de AquiculturaInstituto de PescaUniversidade Estadual Paulista Centro de AquiculturaFAPESP: 05/53070-0Colégio Brasileiro de Patologia Animal - CBPAUniversidade Estadual Paulista (Unesp)Instituto de PescaTeixeira, Patricia C. [UNESP]França, Fernanda M.Rocha, Guilherme C. DaAntonucci, Antônio M.Ferreira, Claudia M.Ranzani-paiva, Maria José T.2014-10-01T13:08:48Z2014-10-01T13:08:48Z2014-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article369-373application/pdfhttp://dx.doi.org/10.1590/S0100-736X2014000400012Pesquisa Veterinária Brasileira. Colégio Brasileiro de Patologia Animal - CBPA, v. 34, n. 4, p. 369-373, 2014.0100-736Xhttp://hdl.handle.net/11449/11008910.1590/S0100-736X2014000400012S0100-736X2014000400012WOS:000338435000012S0100-736X2014000400012.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinária Brasileira0.385info:eu-repo/semantics/openAccess2024-04-09T15:43:25Zoai:repositorio.unesp.br:11449/110089Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:33:03.087104Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Is hypoxia a stressor to American bullfrog tadpoles?
A hipóxia é um estressor para girinos de rã-touro?
title Is hypoxia a stressor to American bullfrog tadpoles?
spellingShingle Is hypoxia a stressor to American bullfrog tadpoles?
Teixeira, Patricia C. [UNESP]
Ranicultura
American bullfrog
Lithobates catesbeianus
tadpole
estresse
cortisol
Frogculture
American bullfrog
Lithobates catesbeianus
tadpole
stress
cortisol
title_short Is hypoxia a stressor to American bullfrog tadpoles?
title_full Is hypoxia a stressor to American bullfrog tadpoles?
title_fullStr Is hypoxia a stressor to American bullfrog tadpoles?
title_full_unstemmed Is hypoxia a stressor to American bullfrog tadpoles?
title_sort Is hypoxia a stressor to American bullfrog tadpoles?
author Teixeira, Patricia C. [UNESP]
author_facet Teixeira, Patricia C. [UNESP]
França, Fernanda M.
Rocha, Guilherme C. Da
Antonucci, Antônio M.
Ferreira, Claudia M.
Ranzani-paiva, Maria José T.
author_role author
author2 França, Fernanda M.
Rocha, Guilherme C. Da
Antonucci, Antônio M.
Ferreira, Claudia M.
Ranzani-paiva, Maria José T.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto de Pesca
dc.contributor.author.fl_str_mv Teixeira, Patricia C. [UNESP]
França, Fernanda M.
Rocha, Guilherme C. Da
Antonucci, Antônio M.
Ferreira, Claudia M.
Ranzani-paiva, Maria José T.
dc.subject.por.fl_str_mv Ranicultura
American bullfrog
Lithobates catesbeianus
tadpole
estresse
cortisol
Frogculture
American bullfrog
Lithobates catesbeianus
tadpole
stress
cortisol
topic Ranicultura
American bullfrog
Lithobates catesbeianus
tadpole
estresse
cortisol
Frogculture
American bullfrog
Lithobates catesbeianus
tadpole
stress
cortisol
description The aim of this study was to evaluate alterations to the physiological profile of cortisol in pro-metamorphose phase tadpoles of Lithobates catesbeianus exposed to hypoxia stressor in a capture experiment and in a crowding experiment. The capture study was performed by the treatments: stress due to individual capture with a hand net, stress due to batch capture with a hand net and stress due to capture by emptying. Three simultaneous replicates was done witch 12 animals were sampled (6 normoxia - immediately blood collection) and 6 hypoxia - blood collection after 15 min of air exposition) in two collection times with 5 days by intervals. The crowding study was performed by the treatments 1 tadpole L-1, 5 tadpoles L-1 and 10 tadpoles L-1. Three simultaneous replicates was done witch 8 animals (4 normoxia and 4 hypoxia) were sampled in the zero moment (ZM) - blood collection before the experiment, 6 animals/treatment (3 normoxia and 3 hypoxia) to 4 and 8 days and 18 animals/treatment (9 normoxia and 9 hypoxia) to 12 days. The average values to plasmatic cortisol varying from 1.7 to 5.1ng mL-1 (capture study) and 1.0 to 4.2ng mL-1 (crowding study). It concludes that the biomarker tested (cortisol) showed no alterations front of the stressor used. Alternatively, a larger response pattern to these stimuli may have been expressed in another level of an unmeasured hormone (corticosterone). And the bullfrog has great ability to adapt to different management compared to other aquatic organisms, which demonstrates the plasticity of these animals.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-01T13:08:48Z
2014-10-01T13:08:48Z
2014-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-736X2014000400012
Pesquisa Veterinária Brasileira. Colégio Brasileiro de Patologia Animal - CBPA, v. 34, n. 4, p. 369-373, 2014.
0100-736X
http://hdl.handle.net/11449/110089
10.1590/S0100-736X2014000400012
S0100-736X2014000400012
WOS:000338435000012
S0100-736X2014000400012.pdf
url http://dx.doi.org/10.1590/S0100-736X2014000400012
http://hdl.handle.net/11449/110089
identifier_str_mv Pesquisa Veterinária Brasileira. Colégio Brasileiro de Patologia Animal - CBPA, v. 34, n. 4, p. 369-373, 2014.
0100-736X
10.1590/S0100-736X2014000400012
S0100-736X2014000400012
WOS:000338435000012
S0100-736X2014000400012.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pesquisa Veterinária Brasileira
0.385
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 369-373
application/pdf
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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