Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill.

Detalhes bibliográficos
Autor(a) principal: Zago, Priscila Megda João Job
Data de Publicação: 2021
Outros Autores: Silva, Gustavo Ratti Da, Amaral, Eduarda Carolina, Barboza, Lorena Neris, Braga, Fernanda De Abreu, Lorençone, Bethânia Rosa [UNESP], Marques, Aline Aparecida Macedo, Moreno, Karyne Garcia Tafarelo, Leite, Patrícia Regina Terço, Veiga, Alan De Almeida, Souza, Lauro Mera De, Souza, Roosevelt Isaias Carvalho, Santos, Ariany Carvalho Dos, Ribeiro-Paes, João Tadeu [UNESP], Gasparotto Junior, Arquimedes, Lívero, Francislaine Aparecida Dos Reis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1155/2021/6580458
http://hdl.handle.net/11449/222962
Resumo: Croton urucurana Baill. is a native Brazilian tree, popularly known as sangra-d'águaor sangue-de-dragão,based on the red resinous sap of the trunk. Its use has been transmitted through generations based on popular tradition that attributes analgesic, anti-inflammatory, and cardioprotective properties to the tree. However, its cardioprotective effects have not yet been scientifically investigated. Thus, the present study investigated the pharmacological response to an ethanol-soluble fraction from the leaves of C. urucurana in Wistar rats exposed to smoking and dyslipidemia, two important cardiovascular risk factors. The extract was evaluated by high-performance liquid chromatography. Wistar rats received a 0.5% cholesterol-enriched diet and were exposed to cigarette smoke (9 cigarettes/day for 10 weeks). During the last 5 weeks, the animals were orally treated with vehicle (negative control group), C. urucurana extract (30, 100, and 300 mg/kg), or simvastatin (2.5 mg/kg) + enalapril (15 mg/kg). One group of rats that was not exposed to these risk factors was also evaluated (basal group). Electrocardiograms and systolic, diastolic, and mean blood pressure were measured. Blood was collected to measure total cholesterol, triglycerides, urea, and creatinine. The heart and kidneys were collected and processed for oxidative status and histopathological evaluation. The phytochemical analysis revealed different classes of flavonoids and condensed tannins. The model induced dyslipidemia and cardiac and renal oxidative stress and increased levels of urea and creatinine in the negative control group. Treatment with the C. urucurana extract (300 mg/kg) and simvastatin + enalapril decreased cholesterol and triglyceride levels. In contrast to simvastatin + enalapril treatment, the C. urucurana extract exerted cardiac and renal antioxidant effects. No alterations of electrocardiograms, blood pressure, or histopathology were observed between groups. These findings indicate that C. urucurana exerts lipid-lowering, renal, and cardioprotective effects against oxidative stress in a preclinical model of multiple risk factors for heart disease.
id UNSP_a03bd0416de61020c69042744ec6f2d7
oai_identifier_str oai:repositorio.unesp.br:11449/222962
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill.Croton urucurana Baill. is a native Brazilian tree, popularly known as sangra-d'águaor sangue-de-dragão,based on the red resinous sap of the trunk. Its use has been transmitted through generations based on popular tradition that attributes analgesic, anti-inflammatory, and cardioprotective properties to the tree. However, its cardioprotective effects have not yet been scientifically investigated. Thus, the present study investigated the pharmacological response to an ethanol-soluble fraction from the leaves of C. urucurana in Wistar rats exposed to smoking and dyslipidemia, two important cardiovascular risk factors. The extract was evaluated by high-performance liquid chromatography. Wistar rats received a 0.5% cholesterol-enriched diet and were exposed to cigarette smoke (9 cigarettes/day for 10 weeks). During the last 5 weeks, the animals were orally treated with vehicle (negative control group), C. urucurana extract (30, 100, and 300 mg/kg), or simvastatin (2.5 mg/kg) + enalapril (15 mg/kg). One group of rats that was not exposed to these risk factors was also evaluated (basal group). Electrocardiograms and systolic, diastolic, and mean blood pressure were measured. Blood was collected to measure total cholesterol, triglycerides, urea, and creatinine. The heart and kidneys were collected and processed for oxidative status and histopathological evaluation. The phytochemical analysis revealed different classes of flavonoids and condensed tannins. The model induced dyslipidemia and cardiac and renal oxidative stress and increased levels of urea and creatinine in the negative control group. Treatment with the C. urucurana extract (300 mg/kg) and simvastatin + enalapril decreased cholesterol and triglyceride levels. In contrast to simvastatin + enalapril treatment, the C. urucurana extract exerted cardiac and renal antioxidant effects. No alterations of electrocardiograms, blood pressure, or histopathology were observed between groups. These findings indicate that C. urucurana exerts lipid-lowering, renal, and cardioprotective effects against oxidative stress in a preclinical model of multiple risk factors for heart disease.Laboratory of Preclinical Research of Natural Products Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention Paranaense UniversityLaboratory of Preclinical Research of Natural Products Post-Graduate Program in Animal Science with Emphasis on Bioactive Products Paranaense UniversityLaboratory of Cardiovascular Pharmacology Faculty of Health Sciences Federal University of Grande DouradosInstitute of Research Pelé Pequeno Príncipe Pequeno Príncipe FacultyLaboratory of Histology Faculty of Health Sciences Federal University of Grande DouradosLaboratory of Genetics and Cell Therapy São Paulo State UniversityLaboratory of Preclinical Research of Natural Products Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention Post-Graduate Program in Animal Science with Emphasis on Bioactive ProductsLaboratory of Genetics and Cell Therapy São Paulo State UniversityParanaense UniversityFederal University of Grande DouradosPequeno Príncipe FacultyUniversidade Estadual Paulista (UNESP)Post-Graduate Program in Animal Science with Emphasis on Bioactive ProductsZago, Priscila Megda João JobSilva, Gustavo Ratti DaAmaral, Eduarda CarolinaBarboza, Lorena NerisBraga, Fernanda De AbreuLorençone, Bethânia Rosa [UNESP]Marques, Aline Aparecida MacedoMoreno, Karyne Garcia TafareloLeite, Patrícia Regina TerçoVeiga, Alan De AlmeidaSouza, Lauro Mera DeSouza, Roosevelt Isaias CarvalhoSantos, Ariany Carvalho DosRibeiro-Paes, João Tadeu [UNESP]Gasparotto Junior, ArquimedesLívero, Francislaine Aparecida Dos Reis2022-04-28T19:47:47Z2022-04-28T19:47:47Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1155/2021/6580458Evidence-based Complementary and Alternative Medicine, v. 2021.1741-42881741-427Xhttp://hdl.handle.net/11449/22296210.1155/2021/65804582-s2.0-85120406157Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEvidence-based Complementary and Alternative Medicineinfo:eu-repo/semantics/openAccess2022-04-28T19:47:47Zoai:repositorio.unesp.br:11449/222962Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:31:15.498714Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill.
title Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill.
spellingShingle Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill.
Zago, Priscila Megda João Job
title_short Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill.
title_full Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill.
title_fullStr Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill.
title_full_unstemmed Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill.
title_sort Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill.
author Zago, Priscila Megda João Job
author_facet Zago, Priscila Megda João Job
Silva, Gustavo Ratti Da
Amaral, Eduarda Carolina
Barboza, Lorena Neris
Braga, Fernanda De Abreu
Lorençone, Bethânia Rosa [UNESP]
Marques, Aline Aparecida Macedo
Moreno, Karyne Garcia Tafarelo
Leite, Patrícia Regina Terço
Veiga, Alan De Almeida
Souza, Lauro Mera De
Souza, Roosevelt Isaias Carvalho
Santos, Ariany Carvalho Dos
Ribeiro-Paes, João Tadeu [UNESP]
Gasparotto Junior, Arquimedes
Lívero, Francislaine Aparecida Dos Reis
author_role author
author2 Silva, Gustavo Ratti Da
Amaral, Eduarda Carolina
Barboza, Lorena Neris
Braga, Fernanda De Abreu
Lorençone, Bethânia Rosa [UNESP]
Marques, Aline Aparecida Macedo
Moreno, Karyne Garcia Tafarelo
Leite, Patrícia Regina Terço
Veiga, Alan De Almeida
Souza, Lauro Mera De
Souza, Roosevelt Isaias Carvalho
Santos, Ariany Carvalho Dos
Ribeiro-Paes, João Tadeu [UNESP]
Gasparotto Junior, Arquimedes
Lívero, Francislaine Aparecida Dos Reis
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Paranaense University
Federal University of Grande Dourados
Pequeno Príncipe Faculty
Universidade Estadual Paulista (UNESP)
Post-Graduate Program in Animal Science with Emphasis on Bioactive Products
dc.contributor.author.fl_str_mv Zago, Priscila Megda João Job
Silva, Gustavo Ratti Da
Amaral, Eduarda Carolina
Barboza, Lorena Neris
Braga, Fernanda De Abreu
Lorençone, Bethânia Rosa [UNESP]
Marques, Aline Aparecida Macedo
Moreno, Karyne Garcia Tafarelo
Leite, Patrícia Regina Terço
Veiga, Alan De Almeida
Souza, Lauro Mera De
Souza, Roosevelt Isaias Carvalho
Santos, Ariany Carvalho Dos
Ribeiro-Paes, João Tadeu [UNESP]
Gasparotto Junior, Arquimedes
Lívero, Francislaine Aparecida Dos Reis
description Croton urucurana Baill. is a native Brazilian tree, popularly known as sangra-d'águaor sangue-de-dragão,based on the red resinous sap of the trunk. Its use has been transmitted through generations based on popular tradition that attributes analgesic, anti-inflammatory, and cardioprotective properties to the tree. However, its cardioprotective effects have not yet been scientifically investigated. Thus, the present study investigated the pharmacological response to an ethanol-soluble fraction from the leaves of C. urucurana in Wistar rats exposed to smoking and dyslipidemia, two important cardiovascular risk factors. The extract was evaluated by high-performance liquid chromatography. Wistar rats received a 0.5% cholesterol-enriched diet and were exposed to cigarette smoke (9 cigarettes/day for 10 weeks). During the last 5 weeks, the animals were orally treated with vehicle (negative control group), C. urucurana extract (30, 100, and 300 mg/kg), or simvastatin (2.5 mg/kg) + enalapril (15 mg/kg). One group of rats that was not exposed to these risk factors was also evaluated (basal group). Electrocardiograms and systolic, diastolic, and mean blood pressure were measured. Blood was collected to measure total cholesterol, triglycerides, urea, and creatinine. The heart and kidneys were collected and processed for oxidative status and histopathological evaluation. The phytochemical analysis revealed different classes of flavonoids and condensed tannins. The model induced dyslipidemia and cardiac and renal oxidative stress and increased levels of urea and creatinine in the negative control group. Treatment with the C. urucurana extract (300 mg/kg) and simvastatin + enalapril decreased cholesterol and triglyceride levels. In contrast to simvastatin + enalapril treatment, the C. urucurana extract exerted cardiac and renal antioxidant effects. No alterations of electrocardiograms, blood pressure, or histopathology were observed between groups. These findings indicate that C. urucurana exerts lipid-lowering, renal, and cardioprotective effects against oxidative stress in a preclinical model of multiple risk factors for heart disease.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
2022-04-28T19:47:47Z
2022-04-28T19:47:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1155/2021/6580458
Evidence-based Complementary and Alternative Medicine, v. 2021.
1741-4288
1741-427X
http://hdl.handle.net/11449/222962
10.1155/2021/6580458
2-s2.0-85120406157
url http://dx.doi.org/10.1155/2021/6580458
http://hdl.handle.net/11449/222962
identifier_str_mv Evidence-based Complementary and Alternative Medicine, v. 2021.
1741-4288
1741-427X
10.1155/2021/6580458
2-s2.0-85120406157
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Evidence-based Complementary and Alternative Medicine
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129081208733696