Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2021/6580458 http://hdl.handle.net/11449/222962 |
Resumo: | Croton urucurana Baill. is a native Brazilian tree, popularly known as sangra-d'águaor sangue-de-dragão,based on the red resinous sap of the trunk. Its use has been transmitted through generations based on popular tradition that attributes analgesic, anti-inflammatory, and cardioprotective properties to the tree. However, its cardioprotective effects have not yet been scientifically investigated. Thus, the present study investigated the pharmacological response to an ethanol-soluble fraction from the leaves of C. urucurana in Wistar rats exposed to smoking and dyslipidemia, two important cardiovascular risk factors. The extract was evaluated by high-performance liquid chromatography. Wistar rats received a 0.5% cholesterol-enriched diet and were exposed to cigarette smoke (9 cigarettes/day for 10 weeks). During the last 5 weeks, the animals were orally treated with vehicle (negative control group), C. urucurana extract (30, 100, and 300 mg/kg), or simvastatin (2.5 mg/kg) + enalapril (15 mg/kg). One group of rats that was not exposed to these risk factors was also evaluated (basal group). Electrocardiograms and systolic, diastolic, and mean blood pressure were measured. Blood was collected to measure total cholesterol, triglycerides, urea, and creatinine. The heart and kidneys were collected and processed for oxidative status and histopathological evaluation. The phytochemical analysis revealed different classes of flavonoids and condensed tannins. The model induced dyslipidemia and cardiac and renal oxidative stress and increased levels of urea and creatinine in the negative control group. Treatment with the C. urucurana extract (300 mg/kg) and simvastatin + enalapril decreased cholesterol and triglyceride levels. In contrast to simvastatin + enalapril treatment, the C. urucurana extract exerted cardiac and renal antioxidant effects. No alterations of electrocardiograms, blood pressure, or histopathology were observed between groups. These findings indicate that C. urucurana exerts lipid-lowering, renal, and cardioprotective effects against oxidative stress in a preclinical model of multiple risk factors for heart disease. |
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Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill.Croton urucurana Baill. is a native Brazilian tree, popularly known as sangra-d'águaor sangue-de-dragão,based on the red resinous sap of the trunk. Its use has been transmitted through generations based on popular tradition that attributes analgesic, anti-inflammatory, and cardioprotective properties to the tree. However, its cardioprotective effects have not yet been scientifically investigated. Thus, the present study investigated the pharmacological response to an ethanol-soluble fraction from the leaves of C. urucurana in Wistar rats exposed to smoking and dyslipidemia, two important cardiovascular risk factors. The extract was evaluated by high-performance liquid chromatography. Wistar rats received a 0.5% cholesterol-enriched diet and were exposed to cigarette smoke (9 cigarettes/day for 10 weeks). During the last 5 weeks, the animals were orally treated with vehicle (negative control group), C. urucurana extract (30, 100, and 300 mg/kg), or simvastatin (2.5 mg/kg) + enalapril (15 mg/kg). One group of rats that was not exposed to these risk factors was also evaluated (basal group). Electrocardiograms and systolic, diastolic, and mean blood pressure were measured. Blood was collected to measure total cholesterol, triglycerides, urea, and creatinine. The heart and kidneys were collected and processed for oxidative status and histopathological evaluation. The phytochemical analysis revealed different classes of flavonoids and condensed tannins. The model induced dyslipidemia and cardiac and renal oxidative stress and increased levels of urea and creatinine in the negative control group. Treatment with the C. urucurana extract (300 mg/kg) and simvastatin + enalapril decreased cholesterol and triglyceride levels. In contrast to simvastatin + enalapril treatment, the C. urucurana extract exerted cardiac and renal antioxidant effects. No alterations of electrocardiograms, blood pressure, or histopathology were observed between groups. These findings indicate that C. urucurana exerts lipid-lowering, renal, and cardioprotective effects against oxidative stress in a preclinical model of multiple risk factors for heart disease.Laboratory of Preclinical Research of Natural Products Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention Paranaense UniversityLaboratory of Preclinical Research of Natural Products Post-Graduate Program in Animal Science with Emphasis on Bioactive Products Paranaense UniversityLaboratory of Cardiovascular Pharmacology Faculty of Health Sciences Federal University of Grande DouradosInstitute of Research Pelé Pequeno Príncipe Pequeno Príncipe FacultyLaboratory of Histology Faculty of Health Sciences Federal University of Grande DouradosLaboratory of Genetics and Cell Therapy São Paulo State UniversityLaboratory of Preclinical Research of Natural Products Post-Graduate Program in Medicinal Plants and Phytotherapeutics in Basic Attention Post-Graduate Program in Animal Science with Emphasis on Bioactive ProductsLaboratory of Genetics and Cell Therapy São Paulo State UniversityParanaense UniversityFederal University of Grande DouradosPequeno Príncipe FacultyUniversidade Estadual Paulista (UNESP)Post-Graduate Program in Animal Science with Emphasis on Bioactive ProductsZago, Priscila Megda João JobSilva, Gustavo Ratti DaAmaral, Eduarda CarolinaBarboza, Lorena NerisBraga, Fernanda De AbreuLorençone, Bethânia Rosa [UNESP]Marques, Aline Aparecida MacedoMoreno, Karyne Garcia TafareloLeite, Patrícia Regina TerçoVeiga, Alan De AlmeidaSouza, Lauro Mera DeSouza, Roosevelt Isaias CarvalhoSantos, Ariany Carvalho DosRibeiro-Paes, João Tadeu [UNESP]Gasparotto Junior, ArquimedesLívero, Francislaine Aparecida Dos Reis2022-04-28T19:47:47Z2022-04-28T19:47:47Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1155/2021/6580458Evidence-based Complementary and Alternative Medicine, v. 2021.1741-42881741-427Xhttp://hdl.handle.net/11449/22296210.1155/2021/65804582-s2.0-85120406157Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEvidence-based Complementary and Alternative Medicineinfo:eu-repo/semantics/openAccess2022-04-28T19:47:47Zoai:repositorio.unesp.br:11449/222962Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:31:15.498714Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill. |
title |
Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill. |
spellingShingle |
Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill. Zago, Priscila Megda João Job |
title_short |
Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill. |
title_full |
Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill. |
title_fullStr |
Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill. |
title_full_unstemmed |
Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill. |
title_sort |
Multiple Risk Factors for Heart Disease: A Challenge to the Ethnopharmacological Use of Croton urucurana Baill. |
author |
Zago, Priscila Megda João Job |
author_facet |
Zago, Priscila Megda João Job Silva, Gustavo Ratti Da Amaral, Eduarda Carolina Barboza, Lorena Neris Braga, Fernanda De Abreu Lorençone, Bethânia Rosa [UNESP] Marques, Aline Aparecida Macedo Moreno, Karyne Garcia Tafarelo Leite, Patrícia Regina Terço Veiga, Alan De Almeida Souza, Lauro Mera De Souza, Roosevelt Isaias Carvalho Santos, Ariany Carvalho Dos Ribeiro-Paes, João Tadeu [UNESP] Gasparotto Junior, Arquimedes Lívero, Francislaine Aparecida Dos Reis |
author_role |
author |
author2 |
Silva, Gustavo Ratti Da Amaral, Eduarda Carolina Barboza, Lorena Neris Braga, Fernanda De Abreu Lorençone, Bethânia Rosa [UNESP] Marques, Aline Aparecida Macedo Moreno, Karyne Garcia Tafarelo Leite, Patrícia Regina Terço Veiga, Alan De Almeida Souza, Lauro Mera De Souza, Roosevelt Isaias Carvalho Santos, Ariany Carvalho Dos Ribeiro-Paes, João Tadeu [UNESP] Gasparotto Junior, Arquimedes Lívero, Francislaine Aparecida Dos Reis |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Paranaense University Federal University of Grande Dourados Pequeno Príncipe Faculty Universidade Estadual Paulista (UNESP) Post-Graduate Program in Animal Science with Emphasis on Bioactive Products |
dc.contributor.author.fl_str_mv |
Zago, Priscila Megda João Job Silva, Gustavo Ratti Da Amaral, Eduarda Carolina Barboza, Lorena Neris Braga, Fernanda De Abreu Lorençone, Bethânia Rosa [UNESP] Marques, Aline Aparecida Macedo Moreno, Karyne Garcia Tafarelo Leite, Patrícia Regina Terço Veiga, Alan De Almeida Souza, Lauro Mera De Souza, Roosevelt Isaias Carvalho Santos, Ariany Carvalho Dos Ribeiro-Paes, João Tadeu [UNESP] Gasparotto Junior, Arquimedes Lívero, Francislaine Aparecida Dos Reis |
description |
Croton urucurana Baill. is a native Brazilian tree, popularly known as sangra-d'águaor sangue-de-dragão,based on the red resinous sap of the trunk. Its use has been transmitted through generations based on popular tradition that attributes analgesic, anti-inflammatory, and cardioprotective properties to the tree. However, its cardioprotective effects have not yet been scientifically investigated. Thus, the present study investigated the pharmacological response to an ethanol-soluble fraction from the leaves of C. urucurana in Wistar rats exposed to smoking and dyslipidemia, two important cardiovascular risk factors. The extract was evaluated by high-performance liquid chromatography. Wistar rats received a 0.5% cholesterol-enriched diet and were exposed to cigarette smoke (9 cigarettes/day for 10 weeks). During the last 5 weeks, the animals were orally treated with vehicle (negative control group), C. urucurana extract (30, 100, and 300 mg/kg), or simvastatin (2.5 mg/kg) + enalapril (15 mg/kg). One group of rats that was not exposed to these risk factors was also evaluated (basal group). Electrocardiograms and systolic, diastolic, and mean blood pressure were measured. Blood was collected to measure total cholesterol, triglycerides, urea, and creatinine. The heart and kidneys were collected and processed for oxidative status and histopathological evaluation. The phytochemical analysis revealed different classes of flavonoids and condensed tannins. The model induced dyslipidemia and cardiac and renal oxidative stress and increased levels of urea and creatinine in the negative control group. Treatment with the C. urucurana extract (300 mg/kg) and simvastatin + enalapril decreased cholesterol and triglyceride levels. In contrast to simvastatin + enalapril treatment, the C. urucurana extract exerted cardiac and renal antioxidant effects. No alterations of electrocardiograms, blood pressure, or histopathology were observed between groups. These findings indicate that C. urucurana exerts lipid-lowering, renal, and cardioprotective effects against oxidative stress in a preclinical model of multiple risk factors for heart disease. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-28T19:47:47Z 2022-04-28T19:47:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2021/6580458 Evidence-based Complementary and Alternative Medicine, v. 2021. 1741-4288 1741-427X http://hdl.handle.net/11449/222962 10.1155/2021/6580458 2-s2.0-85120406157 |
url |
http://dx.doi.org/10.1155/2021/6580458 http://hdl.handle.net/11449/222962 |
identifier_str_mv |
Evidence-based Complementary and Alternative Medicine, v. 2021. 1741-4288 1741-427X 10.1155/2021/6580458 2-s2.0-85120406157 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Evidence-based Complementary and Alternative Medicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129081208733696 |