The comet assay in Ceraeochrysa claveri (Neuroptera: Chrysopidae): A suitable approach for detecting somatic and germ cell genotoxicity induced by agrochemicals

Detalhes bibliográficos
Autor(a) principal: Gastelbondo-Pastrana, Bertha Irina [UNESP]
Data de Publicação: 2019
Outros Autores: Fernandes, Fábio Henrique [UNESP], Salvadori, Daisy Maria Fávero [UNESP], Santos, Daniela Carvalho dos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.chemosphere.2019.06.142
http://hdl.handle.net/11449/189318
Resumo: Some agrochemicals are genotoxic to several organisms. Nevertheless, few protocols are currently available for measuring the toxicogenetic effects of these compounds in target and non-target field-collected species of insects important to agriculture. Herein, we used the species Ceraeochrysa claveri (Neuroptera: Chrysopidae), a non-target predator insect, to investigate the ability of an azadirachtin-based biopesticide (Azamax™) to induce DNA damage. The alkaline version of the comet assay was standardized to evaluate genetic instability caused by the toxicant in somatic (gut) and germ (nurse cells and oocytes) cells of C. claveri. For this, C. claveri larvae were distributed into three groups (10/each) and treated with Azamax™ at 0, 0.3% or 0.5% throughout the larval stage. DNA damage (tail intensity) was measured in adult insects, four days after emerged. The data showed that both doses of Azamax™ (0.3% and 0.5%) were able to significantly (p < 0.05) increase DNA damage in somatic and germ cells of C. claveri. In conclusion, C. claveri (intestinal and ovarian cells) was a sensitive bioindicator for identifying Azamax™ genotoxic potential, whereas the comet assay was a useful tool for detecting the genotoxic hazard of the pesticide in the field-collected insect species. Given that estimation of adverse effects of pollutants on ecosystems is an essential component of environmental risk assessment, the approach used can be recommended to estimate the ecotoxicity of agricultural chemicals.
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spelling The comet assay in Ceraeochrysa claveri (Neuroptera: Chrysopidae): A suitable approach for detecting somatic and germ cell genotoxicity induced by agrochemicalsAzadirachtinBiopesticideDNA damageEcotoxicityGutOvarySome agrochemicals are genotoxic to several organisms. Nevertheless, few protocols are currently available for measuring the toxicogenetic effects of these compounds in target and non-target field-collected species of insects important to agriculture. Herein, we used the species Ceraeochrysa claveri (Neuroptera: Chrysopidae), a non-target predator insect, to investigate the ability of an azadirachtin-based biopesticide (Azamax™) to induce DNA damage. The alkaline version of the comet assay was standardized to evaluate genetic instability caused by the toxicant in somatic (gut) and germ (nurse cells and oocytes) cells of C. claveri. For this, C. claveri larvae were distributed into three groups (10/each) and treated with Azamax™ at 0, 0.3% or 0.5% throughout the larval stage. DNA damage (tail intensity) was measured in adult insects, four days after emerged. The data showed that both doses of Azamax™ (0.3% and 0.5%) were able to significantly (p < 0.05) increase DNA damage in somatic and germ cells of C. claveri. In conclusion, C. claveri (intestinal and ovarian cells) was a sensitive bioindicator for identifying Azamax™ genotoxic potential, whereas the comet assay was a useful tool for detecting the genotoxic hazard of the pesticide in the field-collected insect species. Given that estimation of adverse effects of pollutants on ecosystems is an essential component of environmental risk assessment, the approach used can be recommended to estimate the ecotoxicity of agricultural chemicals.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Laboratory of Insects Department of Morphology Institute of Biosciences of Botucatu UNESP - São Paulo State UniversityLaboratory of Toxicogenomic and Nutrigenomic Department of Pathology Medical School UNESP - São Paulo State UniversityElectron Microscopy Center Institute of Biosciences of Botucatu UNESP - São Paulo State UniversityLaboratory of Insects Department of Morphology Institute of Biosciences of Botucatu UNESP - São Paulo State UniversityLaboratory of Toxicogenomic and Nutrigenomic Department of Pathology Medical School UNESP - São Paulo State UniversityElectron Microscopy Center Institute of Biosciences of Botucatu UNESP - São Paulo State UniversityCNPq: 406334/2018-8Universidade Estadual Paulista (Unesp)Gastelbondo-Pastrana, Bertha Irina [UNESP]Fernandes, Fábio Henrique [UNESP]Salvadori, Daisy Maria Fávero [UNESP]Santos, Daniela Carvalho dos [UNESP]2019-10-06T16:36:52Z2019-10-06T16:36:52Z2019-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article70-75http://dx.doi.org/10.1016/j.chemosphere.2019.06.142Chemosphere, v. 235, p. 70-75.1879-12980045-6535http://hdl.handle.net/11449/18931810.1016/j.chemosphere.2019.06.1422-s2.0-85067898803Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosphereinfo:eu-repo/semantics/openAccess2024-09-03T13:15:38Zoai:repositorio.unesp.br:11449/189318Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:15:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The comet assay in Ceraeochrysa claveri (Neuroptera: Chrysopidae): A suitable approach for detecting somatic and germ cell genotoxicity induced by agrochemicals
title The comet assay in Ceraeochrysa claveri (Neuroptera: Chrysopidae): A suitable approach for detecting somatic and germ cell genotoxicity induced by agrochemicals
spellingShingle The comet assay in Ceraeochrysa claveri (Neuroptera: Chrysopidae): A suitable approach for detecting somatic and germ cell genotoxicity induced by agrochemicals
Gastelbondo-Pastrana, Bertha Irina [UNESP]
Azadirachtin
Biopesticide
DNA damage
Ecotoxicity
Gut
Ovary
title_short The comet assay in Ceraeochrysa claveri (Neuroptera: Chrysopidae): A suitable approach for detecting somatic and germ cell genotoxicity induced by agrochemicals
title_full The comet assay in Ceraeochrysa claveri (Neuroptera: Chrysopidae): A suitable approach for detecting somatic and germ cell genotoxicity induced by agrochemicals
title_fullStr The comet assay in Ceraeochrysa claveri (Neuroptera: Chrysopidae): A suitable approach for detecting somatic and germ cell genotoxicity induced by agrochemicals
title_full_unstemmed The comet assay in Ceraeochrysa claveri (Neuroptera: Chrysopidae): A suitable approach for detecting somatic and germ cell genotoxicity induced by agrochemicals
title_sort The comet assay in Ceraeochrysa claveri (Neuroptera: Chrysopidae): A suitable approach for detecting somatic and germ cell genotoxicity induced by agrochemicals
author Gastelbondo-Pastrana, Bertha Irina [UNESP]
author_facet Gastelbondo-Pastrana, Bertha Irina [UNESP]
Fernandes, Fábio Henrique [UNESP]
Salvadori, Daisy Maria Fávero [UNESP]
Santos, Daniela Carvalho dos [UNESP]
author_role author
author2 Fernandes, Fábio Henrique [UNESP]
Salvadori, Daisy Maria Fávero [UNESP]
Santos, Daniela Carvalho dos [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Gastelbondo-Pastrana, Bertha Irina [UNESP]
Fernandes, Fábio Henrique [UNESP]
Salvadori, Daisy Maria Fávero [UNESP]
Santos, Daniela Carvalho dos [UNESP]
dc.subject.por.fl_str_mv Azadirachtin
Biopesticide
DNA damage
Ecotoxicity
Gut
Ovary
topic Azadirachtin
Biopesticide
DNA damage
Ecotoxicity
Gut
Ovary
description Some agrochemicals are genotoxic to several organisms. Nevertheless, few protocols are currently available for measuring the toxicogenetic effects of these compounds in target and non-target field-collected species of insects important to agriculture. Herein, we used the species Ceraeochrysa claveri (Neuroptera: Chrysopidae), a non-target predator insect, to investigate the ability of an azadirachtin-based biopesticide (Azamax™) to induce DNA damage. The alkaline version of the comet assay was standardized to evaluate genetic instability caused by the toxicant in somatic (gut) and germ (nurse cells and oocytes) cells of C. claveri. For this, C. claveri larvae were distributed into three groups (10/each) and treated with Azamax™ at 0, 0.3% or 0.5% throughout the larval stage. DNA damage (tail intensity) was measured in adult insects, four days after emerged. The data showed that both doses of Azamax™ (0.3% and 0.5%) were able to significantly (p < 0.05) increase DNA damage in somatic and germ cells of C. claveri. In conclusion, C. claveri (intestinal and ovarian cells) was a sensitive bioindicator for identifying Azamax™ genotoxic potential, whereas the comet assay was a useful tool for detecting the genotoxic hazard of the pesticide in the field-collected insect species. Given that estimation of adverse effects of pollutants on ecosystems is an essential component of environmental risk assessment, the approach used can be recommended to estimate the ecotoxicity of agricultural chemicals.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:36:52Z
2019-10-06T16:36:52Z
2019-11-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.chemosphere.2019.06.142
Chemosphere, v. 235, p. 70-75.
1879-1298
0045-6535
http://hdl.handle.net/11449/189318
10.1016/j.chemosphere.2019.06.142
2-s2.0-85067898803
url http://dx.doi.org/10.1016/j.chemosphere.2019.06.142
http://hdl.handle.net/11449/189318
identifier_str_mv Chemosphere, v. 235, p. 70-75.
1879-1298
0045-6535
10.1016/j.chemosphere.2019.06.142
2-s2.0-85067898803
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chemosphere
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 70-75
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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