Analyses of biofilm on implant abutment surfaces coating with diamond-like carbon and biocompatibility

• Autor(a) principal: Huacho, Patricia Milagros Maquera [UNESP]
• Data de Publicação: 2017
• Outros Autores: Nogueira, Marianne N. Marques [UNESP], Basso, Fernanda G. [UNESP], Junior, Miguel Jafelicci [UNESP], Francisconi, Renata S. [UNESP], Spolidorio, Denise M. P. [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/0103-6440201601136; http://hdl.handle.net/11449/175007
• topic: Diamond-like carbon; Implantology; In vitro; Microbiology; Prosthodontics

The aim of this study was to evaluate the surface free energy (SFE), wetting and surface properties as well as antimicrobial, adhesion and biocompatibility properties of diamondlike carbon (DLC)-coated surfaces. In addition, the leakage of Escherichia coli through the abutment-dental implant interface was also calculated. SFE was calculated from contact angle values; Ra was measured before and after DLC coating. Antimicrobial and adhesion properties against E. coli and cytotoxicity of DLC with human keratinocytes (HaCaT) were evaluated. Further, the ability of DLC-coated surfaces to prevent the migration of E. coli into the external hexagonal implant interface was also evaluated. A sterile technique was used for the semi-quantitative polymerase chain reaction (semi-quantitative PCR). The surfaces showed slight decreases in cell viability (p<0.05), while the SFE, Ra, bacterial adhesion, antimicrobial, and bacterial infiltration tests showed no statistically significant differences (p>0.05). It was concluded that DLC was shown to be a biocompatible material with mild cytotoxicity that did not show changes in Ra, SFE, bacterial adhesion or antimicrobial properties and did not inhibit the infiltration of E. coli into the abutment-dental implant interface.