DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the Squalus Genus (Squaliformes: Squalidae)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/d14070544 http://hdl.handle.net/11449/241366 |
Resumo: | The Squalus genus comprises a group of small demersal sharks occurring circumglobally, popularly known as dogfish sharks. This genus exhibits a conserved morphology, thus making correct morphological identification difficult. Considering these taxonomic problems and the scarcity of molecular data, the present study aimed to identify Squalus genus MOTUs, using DNA barcoding for species delimitation via ABGD (automatic barcode gap discovery), PTP (Poisson tree process), and GMYC (general mixed Yule coalescent) employing the mitochondrial COI gene. A total of 69 sequences were generated from samples obtained from the American coast in both the Atlantic and Pacific Oceans. The ABGD analysis was the most conservative among the three applied delimitations, indicating three taxonomic units, while the PTP analysis revealed nine MOTUs, with two conflicting units noted between S. clarkae + S. mitsukurii and S. albicaudus + S. cubensis. The GMYC analysis indicated an excessive division, with S. acanthias and S. mitsukurii subdivided into six MOTUs each and S. blainville, into four. These findings demonstrated that Squalus presents a complex of previously defined species, with misidentified samples deposited in databases leading to difficulties in analyzing the real distribution and diversity of species belonging to this genus. Thus, further efforts to highlight possible new species are recommended. |
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DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the Squalus Genus (Squaliformes: Squalidae)COIdogsharksElasmobranchiimitochondrial DNAspecies identificationThe Squalus genus comprises a group of small demersal sharks occurring circumglobally, popularly known as dogfish sharks. This genus exhibits a conserved morphology, thus making correct morphological identification difficult. Considering these taxonomic problems and the scarcity of molecular data, the present study aimed to identify Squalus genus MOTUs, using DNA barcoding for species delimitation via ABGD (automatic barcode gap discovery), PTP (Poisson tree process), and GMYC (general mixed Yule coalescent) employing the mitochondrial COI gene. A total of 69 sequences were generated from samples obtained from the American coast in both the Atlantic and Pacific Oceans. The ABGD analysis was the most conservative among the three applied delimitations, indicating three taxonomic units, while the PTP analysis revealed nine MOTUs, with two conflicting units noted between S. clarkae + S. mitsukurii and S. albicaudus + S. cubensis. The GMYC analysis indicated an excessive division, with S. acanthias and S. mitsukurii subdivided into six MOTUs each and S. blainville, into four. These findings demonstrated that Squalus presents a complex of previously defined species, with misidentified samples deposited in databases leading to difficulties in analyzing the real distribution and diversity of species belonging to this genus. Thus, further efforts to highlight possible new species are recommended.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratório de Biologia e Genética de Peixes Instituto de Biociências de Botucatu Universidade Estadual PaulistaLaboratório de Oceanografia Pesqueira Universidade Federal Rural de PernambucoLaboratório de Biologia e Tecnologia Pesqueira Universidade Federal do Rio de JaneiroAcervo Zoológico da Universidade Santa Cecília Universidade Santa CecíliaGrupo de Biotaxonomía Morfológica y Molecular de Peces Departamento de Biología Facultad de Ciencias Exactas y Naturales Instituto de Investigaciones Marinas y Costeras, Funes 3250Consejo Nacional de Investigaciones Científicas y Técnicas CABA AAJLaboratório de Biologia e Genética de Peixes Instituto de Biociências de Botucatu Universidade Estadual PaulistaCNPq: 107761/2019-0 to A.A.A.FAPESP: 2014/26508-3FAPESP: 2016/09204-6FAPESP: 2018/20610-1CNPq: 306054/2006-0Universidade Estadual Paulista (UNESP)Universidade Federal Rural de PernambucoUniversidade Federal do Rio de Janeiro (UFRJ)Universidade Santa CecíliaInstituto de Investigaciones Marinas y CosterasCABA AAJAriza, Ailton A. [UNESP]Adachi, Aisni M. C. L. [UNESP]Roque, PollyanaHazin, Fabio H. V.Vianna, MarceloRotundo, Matheus M.Delpiani, Sergio M.de Astarloa, Juan M. DíazDelpiani, GabrielaOliveira, Claudio [UNESP]Foresti, Fausto [UNESP]Cruz, Vanessa P. [UNESP]2023-03-01T20:58:49Z2023-03-01T20:58:49Z2022-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/d14070544Diversity, v. 14, n. 7, 2022.1424-2818http://hdl.handle.net/11449/24136610.3390/d140705442-s2.0-85134490918Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDiversityinfo:eu-repo/semantics/openAccess2023-03-01T20:58:50Zoai:repositorio.unesp.br:11449/241366Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:12:24.097622Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the Squalus Genus (Squaliformes: Squalidae) |
title |
DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the Squalus Genus (Squaliformes: Squalidae) |
spellingShingle |
DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the Squalus Genus (Squaliformes: Squalidae) Ariza, Ailton A. [UNESP] COI dogsharks Elasmobranchii mitochondrial DNA species identification |
title_short |
DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the Squalus Genus (Squaliformes: Squalidae) |
title_full |
DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the Squalus Genus (Squaliformes: Squalidae) |
title_fullStr |
DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the Squalus Genus (Squaliformes: Squalidae) |
title_full_unstemmed |
DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the Squalus Genus (Squaliformes: Squalidae) |
title_sort |
DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the Squalus Genus (Squaliformes: Squalidae) |
author |
Ariza, Ailton A. [UNESP] |
author_facet |
Ariza, Ailton A. [UNESP] Adachi, Aisni M. C. L. [UNESP] Roque, Pollyana Hazin, Fabio H. V. Vianna, Marcelo Rotundo, Matheus M. Delpiani, Sergio M. de Astarloa, Juan M. Díaz Delpiani, Gabriela Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] Cruz, Vanessa P. [UNESP] |
author_role |
author |
author2 |
Adachi, Aisni M. C. L. [UNESP] Roque, Pollyana Hazin, Fabio H. V. Vianna, Marcelo Rotundo, Matheus M. Delpiani, Sergio M. de Astarloa, Juan M. Díaz Delpiani, Gabriela Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] Cruz, Vanessa P. [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal Rural de Pernambuco Universidade Federal do Rio de Janeiro (UFRJ) Universidade Santa Cecília Instituto de Investigaciones Marinas y Costeras CABA AAJ |
dc.contributor.author.fl_str_mv |
Ariza, Ailton A. [UNESP] Adachi, Aisni M. C. L. [UNESP] Roque, Pollyana Hazin, Fabio H. V. Vianna, Marcelo Rotundo, Matheus M. Delpiani, Sergio M. de Astarloa, Juan M. Díaz Delpiani, Gabriela Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] Cruz, Vanessa P. [UNESP] |
dc.subject.por.fl_str_mv |
COI dogsharks Elasmobranchii mitochondrial DNA species identification |
topic |
COI dogsharks Elasmobranchii mitochondrial DNA species identification |
description |
The Squalus genus comprises a group of small demersal sharks occurring circumglobally, popularly known as dogfish sharks. This genus exhibits a conserved morphology, thus making correct morphological identification difficult. Considering these taxonomic problems and the scarcity of molecular data, the present study aimed to identify Squalus genus MOTUs, using DNA barcoding for species delimitation via ABGD (automatic barcode gap discovery), PTP (Poisson tree process), and GMYC (general mixed Yule coalescent) employing the mitochondrial COI gene. A total of 69 sequences were generated from samples obtained from the American coast in both the Atlantic and Pacific Oceans. The ABGD analysis was the most conservative among the three applied delimitations, indicating three taxonomic units, while the PTP analysis revealed nine MOTUs, with two conflicting units noted between S. clarkae + S. mitsukurii and S. albicaudus + S. cubensis. The GMYC analysis indicated an excessive division, with S. acanthias and S. mitsukurii subdivided into six MOTUs each and S. blainville, into four. These findings demonstrated that Squalus presents a complex of previously defined species, with misidentified samples deposited in databases leading to difficulties in analyzing the real distribution and diversity of species belonging to this genus. Thus, further efforts to highlight possible new species are recommended. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-01 2023-03-01T20:58:49Z 2023-03-01T20:58:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/d14070544 Diversity, v. 14, n. 7, 2022. 1424-2818 http://hdl.handle.net/11449/241366 10.3390/d14070544 2-s2.0-85134490918 |
url |
http://dx.doi.org/10.3390/d14070544 http://hdl.handle.net/11449/241366 |
identifier_str_mv |
Diversity, v. 14, n. 7, 2022. 1424-2818 10.3390/d14070544 2-s2.0-85134490918 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Diversity |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129595459764224 |