Morphological and molecular characterization of stigonema jureiensis sp. Nov. (nostocales, cyanobacteria) from the atlantic rainforest, são paulo, brazil

Detalhes bibliográficos
Autor(a) principal: Hentschke, Guilherme S.
Data de Publicação: 2019
Outros Autores: Rigonato, Janaína [UNESP], Genuário, Diego B., Laughinghouse, Haywood D., Sant‘anna, Célia L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.5507/fot.2019.009
http://hdl.handle.net/11449/199596
Resumo: Tropical regions are considered hotspots of biodiversity, and the genus Stigonema has been underexplored in all world, mainly due to the difficulties of culturing. In this study, we sequenced the 16S rRNA gene of a natural Stigonema population from the tropical Atlantic Rainforest (São Paulo, Brazil) and describe S. jureiensis sp. nov. based on phylogenetic and morphological data. For the molecular analysis, the genomic DNA was isolated from aliquots of the population, which were previously manually cleaned under a microscope, using a point–tapered Pasteur pipette to remove other organisms and debris. For morphological analysis, 30 individuals were analyzed and photographed using microscope. Both maximum likelihood and Bayesian phylogenies support S. jureiensis as a new species, and morphological analysis revealed the presence of a special type of mosaic ornate sheaths.
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spelling Morphological and molecular characterization of stigonema jureiensis sp. Nov. (nostocales, cyanobacteria) from the atlantic rainforest, são paulo, brazilBiodiversityMicroorganismPolyphasic approachTaxonomyTerrestrial habitatsTropical environmentsTropical regions are considered hotspots of biodiversity, and the genus Stigonema has been underexplored in all world, mainly due to the difficulties of culturing. In this study, we sequenced the 16S rRNA gene of a natural Stigonema population from the tropical Atlantic Rainforest (São Paulo, Brazil) and describe S. jureiensis sp. nov. based on phylogenetic and morphological data. For the molecular analysis, the genomic DNA was isolated from aliquots of the population, which were previously manually cleaned under a microscope, using a point–tapered Pasteur pipette to remove other organisms and debris. For morphological analysis, 30 individuals were analyzed and photographed using microscope. Both maximum likelihood and Bayesian phylogenies support S. jureiensis as a new species, and morphological analysis revealed the presence of a special type of mosaic ornate sheaths.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação para a Ciência e a TecnologiaInterdisciplinary Centre of Marine and Environmental Research – CIIMAR Universidade do Porto, Av. General Norton de Matos S/NDepartment of Zoology and Botany Instituto de Biociências Letras e Ciências Exatas (UNESP), R. Cristóvão Colombo, 2265, Jardim NazarethCEA Centre de Sequençage Genoscope Institut de Biologie François JacobEMBRAPA Environment Laboratory of Environmental MicrobiologyAgronomy Department Fort Lauderdale Research and Education Center University of Florida / IFAS, 3205 College AvenueInstitute of Botany Laboratory of Phycology, Av. Miguel Stéfano, 3687, Água FundaDepartment of Zoology and Botany Instituto de Biociências Letras e Ciências Exatas (UNESP), R. Cristóvão Colombo, 2265, Jardim NazarethFAPESP: 2014/26131–7CNPq: Multi/04423/2019Fundação para a Ciência e a Tecnologia: Multi/04423/2019Universidade do PortoUniversidade Estadual Paulista (Unesp)Institut de Biologie François JacobEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)University of Florida / IFASLaboratory of PhycologyHentschke, Guilherme S.Rigonato, Janaína [UNESP]Genuário, Diego B.Laughinghouse, Haywood D.Sant‘anna, Célia L.2020-12-12T01:44:11Z2020-12-12T01:44:11Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article185-191http://dx.doi.org/10.5507/fot.2019.009Fottea, v. 19, n. 2, p. 185-191, 2019.1805-49271802-5439http://hdl.handle.net/11449/19959610.5507/fot.2019.0092-s2.0-85074417770Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFotteainfo:eu-repo/semantics/openAccess2021-10-23T08:32:02Zoai:repositorio.unesp.br:11449/199596Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:53:24.473127Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Morphological and molecular characterization of stigonema jureiensis sp. Nov. (nostocales, cyanobacteria) from the atlantic rainforest, são paulo, brazil
title Morphological and molecular characterization of stigonema jureiensis sp. Nov. (nostocales, cyanobacteria) from the atlantic rainforest, são paulo, brazil
spellingShingle Morphological and molecular characterization of stigonema jureiensis sp. Nov. (nostocales, cyanobacteria) from the atlantic rainforest, são paulo, brazil
Hentschke, Guilherme S.
Biodiversity
Microorganism
Polyphasic approach
Taxonomy
Terrestrial habitats
Tropical environments
title_short Morphological and molecular characterization of stigonema jureiensis sp. Nov. (nostocales, cyanobacteria) from the atlantic rainforest, são paulo, brazil
title_full Morphological and molecular characterization of stigonema jureiensis sp. Nov. (nostocales, cyanobacteria) from the atlantic rainforest, são paulo, brazil
title_fullStr Morphological and molecular characterization of stigonema jureiensis sp. Nov. (nostocales, cyanobacteria) from the atlantic rainforest, são paulo, brazil
title_full_unstemmed Morphological and molecular characterization of stigonema jureiensis sp. Nov. (nostocales, cyanobacteria) from the atlantic rainforest, são paulo, brazil
title_sort Morphological and molecular characterization of stigonema jureiensis sp. Nov. (nostocales, cyanobacteria) from the atlantic rainforest, são paulo, brazil
author Hentschke, Guilherme S.
author_facet Hentschke, Guilherme S.
Rigonato, Janaína [UNESP]
Genuário, Diego B.
Laughinghouse, Haywood D.
Sant‘anna, Célia L.
author_role author
author2 Rigonato, Janaína [UNESP]
Genuário, Diego B.
Laughinghouse, Haywood D.
Sant‘anna, Célia L.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Porto
Universidade Estadual Paulista (Unesp)
Institut de Biologie François Jacob
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
University of Florida / IFAS
Laboratory of Phycology
dc.contributor.author.fl_str_mv Hentschke, Guilherme S.
Rigonato, Janaína [UNESP]
Genuário, Diego B.
Laughinghouse, Haywood D.
Sant‘anna, Célia L.
dc.subject.por.fl_str_mv Biodiversity
Microorganism
Polyphasic approach
Taxonomy
Terrestrial habitats
Tropical environments
topic Biodiversity
Microorganism
Polyphasic approach
Taxonomy
Terrestrial habitats
Tropical environments
description Tropical regions are considered hotspots of biodiversity, and the genus Stigonema has been underexplored in all world, mainly due to the difficulties of culturing. In this study, we sequenced the 16S rRNA gene of a natural Stigonema population from the tropical Atlantic Rainforest (São Paulo, Brazil) and describe S. jureiensis sp. nov. based on phylogenetic and morphological data. For the molecular analysis, the genomic DNA was isolated from aliquots of the population, which were previously manually cleaned under a microscope, using a point–tapered Pasteur pipette to remove other organisms and debris. For morphological analysis, 30 individuals were analyzed and photographed using microscope. Both maximum likelihood and Bayesian phylogenies support S. jureiensis as a new species, and morphological analysis revealed the presence of a special type of mosaic ornate sheaths.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
2020-12-12T01:44:11Z
2020-12-12T01:44:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5507/fot.2019.009
Fottea, v. 19, n. 2, p. 185-191, 2019.
1805-4927
1802-5439
http://hdl.handle.net/11449/199596
10.5507/fot.2019.009
2-s2.0-85074417770
url http://dx.doi.org/10.5507/fot.2019.009
http://hdl.handle.net/11449/199596
identifier_str_mv Fottea, v. 19, n. 2, p. 185-191, 2019.
1805-4927
1802-5439
10.5507/fot.2019.009
2-s2.0-85074417770
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fottea
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 185-191
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129562132873216

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