Modulação de proteínas regulatórias e juncionais da barreira hematotesticular em células de Sertoli humanas (HSec) expostas ao Monobutil ftalato (MBP) e ao Bisfenl A (BPA)

• Autor(a) principal: Freitas, André Teves Aquino Gonçalves de [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/128058; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/21-09-2015/000847999.pdf
• topic: Desreguladores endócrinos; Sertoli, Células de; Espermatogenese; Barreira hematotesticular; Blood-Testis Barrier; Sertoli cells

Sertoli cells, found in the seminiferous epithelium, form the blood-testis barrier via their gap and tight junctions, responsible for providing a protected environment and coordinate the spermatogenic process, essential for male fertility maintenance. The dynamics of the blood-testis barrier formation is directly related to the balance between synthesis and degradation of specialized molecules, and is influenced by androgen regulation and specific growth factors such as TGF-β2, being particularly sensitive to environmental factors. Exposure to endocrine disruptors (EDs), which interfere in the barrier modulation, can lead to infertility. Currently the male factor may be responsible for 30-50% of infertility cases, being the establishment of the relationship between exposure to EDs and the process of gametes formation of great importance. This study aimed to correlate the action of two EDs, monobutyl phthalate (MBP) and bisphenol A (BPA) in different periods of exposure, from a dose of low toxicity on the HSec lineage of human Sertoli cells (in vitro) and its possible action on proteins of blood-testis barrier and regulatory proteins involved in modulation of degradation and synthesis of this barrier. To this end, cells were cultured in HSec lineage specific medium and exposed to MBP and BPA in low doses for 6 and 48 hours. Then, cells were isolated and subjected to protein extraction. Proteins analyzed were: occludin, Ncadherin, β-catenin and Androgen Receptor (AR) by Western blot. The results were compared for the two agents in different exposure times to non-treated cells, showing that both agents were able to reduce the expression of important BTB proteins, presenting potential for disrupting the structure and maintenance of the barrier and, consequently, sperm production and male fertility