Antitumoral activity of micellar solutions containing allyl isothiocyanate: an in vitro study
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.30827/ars.v62i1.15845 http://hdl.handle.net/11449/209086 |
Resumo: | Introduction: Several natural products exhibit promising antineoplastic activity against bladder cancer cells, including allyl isothiocyanate (AITC). However, the AITC irritates the mucous membranes and induces eczematous or vesicular skin reactions. Thus, pharmaceutical formulations are necessary to overcome these problems. The aim was to develop micellar solutions containing AITC and investigate their antitumoral activity in bladder carcinoma cell lines. Method: The micellar solutions were prepared by cold dispersion method. Subsequently, we evaluated cytotoxicity, cell proliferation, cell cycle kinetics and long-term effects of micelles in bladder cancer cells. Results: Cytotoxicity and cell proliferation assays showed there was an increase in AITC activity when it was encapsulated in micelles. We also observed cell cycle arrest in the S phase after treatment with AITC-micelles. Furthermore, the formulation was able to maintain the long-term effects of free AITC. Conclusions: The micellar solutions developed can become an interesting approach for administration of AITC in the treatment of bladder cancer. |
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Repositório Institucional da UNESP |
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Antitumoral activity of micellar solutions containing allyl isothiocyanate: an in vitro studyallyl isothiocyanatebladder cancermicellespoloxamerIntroduction: Several natural products exhibit promising antineoplastic activity against bladder cancer cells, including allyl isothiocyanate (AITC). However, the AITC irritates the mucous membranes and induces eczematous or vesicular skin reactions. Thus, pharmaceutical formulations are necessary to overcome these problems. The aim was to develop micellar solutions containing AITC and investigate their antitumoral activity in bladder carcinoma cell lines. Method: The micellar solutions were prepared by cold dispersion method. Subsequently, we evaluated cytotoxicity, cell proliferation, cell cycle kinetics and long-term effects of micelles in bladder cancer cells. Results: Cytotoxicity and cell proliferation assays showed there was an increase in AITC activity when it was encapsulated in micelles. We also observed cell cycle arrest in the S phase after treatment with AITC-micelles. Furthermore, the formulation was able to maintain the long-term effects of free AITC. Conclusions: The micellar solutions developed can become an interesting approach for administration of AITC in the treatment of bladder cancer.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Univ Fed Ouro Preto, Lab Pesquisas Clin, Ouro Preto, BrazilUniv Fed Ouro Preto, Lab Bioquim & Biol Mol, Ouro Preto, BrazilUniv Estadual Paulista, Lab Toxicogen & Nutrigen, Botucatu, SP, BrazilUniv Fed Ouro Preto, Lab Fitotecnol, Ouro Preto, BrazilUniv Fed Pelotas, Lab Tecnol Farmaceut, Pelotas, RS, BrazilUniv Estadual Paulista, Lab Toxicogen & Nutrigen, Botucatu, SP, BrazilFAPEMIG: PPM-00282-11FAPEMIG: CBB-APQ-01497-14Univ Granada, EditorialUniv Fed Ouro PretoUniversidade Estadual Paulista (Unesp)Univ Fed PelotasAlmeida, Tamires CunhaOliveira, Daiane Teixeira deVentura Savio, Andre Luiz [UNESP]Perasoli, Fernanda BarcanteSilva, Glenda Nicioli daBarichello, Jose Mario2021-06-25T11:48:11Z2021-06-25T11:48:11Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article40-51http://dx.doi.org/10.30827/ars.v62i1.15845Ars Pharmaceutica. Granada: Univ Granada, Editorial, v. 62, n. 1, p. 40-51, 2021.0004-2927http://hdl.handle.net/11449/20908610.30827/ars.v62i1.15845WOS:000605905800003Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArs Pharmaceuticainfo:eu-repo/semantics/openAccess2021-10-23T19:23:31Zoai:repositorio.unesp.br:11449/209086Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:23:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antitumoral activity of micellar solutions containing allyl isothiocyanate: an in vitro study |
title |
Antitumoral activity of micellar solutions containing allyl isothiocyanate: an in vitro study |
spellingShingle |
Antitumoral activity of micellar solutions containing allyl isothiocyanate: an in vitro study Almeida, Tamires Cunha allyl isothiocyanate bladder cancer micelles poloxamer |
title_short |
Antitumoral activity of micellar solutions containing allyl isothiocyanate: an in vitro study |
title_full |
Antitumoral activity of micellar solutions containing allyl isothiocyanate: an in vitro study |
title_fullStr |
Antitumoral activity of micellar solutions containing allyl isothiocyanate: an in vitro study |
title_full_unstemmed |
Antitumoral activity of micellar solutions containing allyl isothiocyanate: an in vitro study |
title_sort |
Antitumoral activity of micellar solutions containing allyl isothiocyanate: an in vitro study |
author |
Almeida, Tamires Cunha |
author_facet |
Almeida, Tamires Cunha Oliveira, Daiane Teixeira de Ventura Savio, Andre Luiz [UNESP] Perasoli, Fernanda Barcante Silva, Glenda Nicioli da Barichello, Jose Mario |
author_role |
author |
author2 |
Oliveira, Daiane Teixeira de Ventura Savio, Andre Luiz [UNESP] Perasoli, Fernanda Barcante Silva, Glenda Nicioli da Barichello, Jose Mario |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Univ Fed Ouro Preto Universidade Estadual Paulista (Unesp) Univ Fed Pelotas |
dc.contributor.author.fl_str_mv |
Almeida, Tamires Cunha Oliveira, Daiane Teixeira de Ventura Savio, Andre Luiz [UNESP] Perasoli, Fernanda Barcante Silva, Glenda Nicioli da Barichello, Jose Mario |
dc.subject.por.fl_str_mv |
allyl isothiocyanate bladder cancer micelles poloxamer |
topic |
allyl isothiocyanate bladder cancer micelles poloxamer |
description |
Introduction: Several natural products exhibit promising antineoplastic activity against bladder cancer cells, including allyl isothiocyanate (AITC). However, the AITC irritates the mucous membranes and induces eczematous or vesicular skin reactions. Thus, pharmaceutical formulations are necessary to overcome these problems. The aim was to develop micellar solutions containing AITC and investigate their antitumoral activity in bladder carcinoma cell lines. Method: The micellar solutions were prepared by cold dispersion method. Subsequently, we evaluated cytotoxicity, cell proliferation, cell cycle kinetics and long-term effects of micelles in bladder cancer cells. Results: Cytotoxicity and cell proliferation assays showed there was an increase in AITC activity when it was encapsulated in micelles. We also observed cell cycle arrest in the S phase after treatment with AITC-micelles. Furthermore, the formulation was able to maintain the long-term effects of free AITC. Conclusions: The micellar solutions developed can become an interesting approach for administration of AITC in the treatment of bladder cancer. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:48:11Z 2021-06-25T11:48:11Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.30827/ars.v62i1.15845 Ars Pharmaceutica. Granada: Univ Granada, Editorial, v. 62, n. 1, p. 40-51, 2021. 0004-2927 http://hdl.handle.net/11449/209086 10.30827/ars.v62i1.15845 WOS:000605905800003 |
url |
http://dx.doi.org/10.30827/ars.v62i1.15845 http://hdl.handle.net/11449/209086 |
identifier_str_mv |
Ars Pharmaceutica. Granada: Univ Granada, Editorial, v. 62, n. 1, p. 40-51, 2021. 0004-2927 10.30827/ars.v62i1.15845 WOS:000605905800003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ars Pharmaceutica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
40-51 |
dc.publisher.none.fl_str_mv |
Univ Granada, Editorial |
publisher.none.fl_str_mv |
Univ Granada, Editorial |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965196726304768 |