Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts

Detalhes bibliográficos
Autor(a) principal: Cury, Sarah Santiloni [UNESP]
Data de Publicação: 2018
Outros Autores: Oliveira, Miriane [UNESP], Síbio, Maria Teresa [UNESP], Clara, Sueli [UNESP], Luvizotto, Renata De Azevedo Melo [UNESP], Conde, Sandro, Jorge, Edson Nacib [UNESP], Nunes, Vania Dos Santos [UNESP], Nogueira, Célia Regina [UNESP], Mazeto, Gláucia Maria Ferreira da Silva [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.20945/2359-3997000000044
http://hdl.handle.net/11449/176653
Resumo: Objective: Graves’ ophthalmopathy (GO) is an autoimmune disease that leads to ocular proptosis caused by fat accumulation and inflammation, and the main treatment is corticosteroid therapy. Retinoid acid receptor-alpha (RARα) seems to be associated with inflammation and adipocyte differentiation. This study aimed to assess the effect of glucocorticoid treatment on orbital fibroblasts of GO patient treated or not with different glucocorticoid doses. Materials and methods: Orbital fibroblasts collected during orbital decompression of a female patient with moderately severe/severe GO were cultivated and treated with 10 nM and 100 nM dexamethasone (Dex). RARα gene expression in the treated and untreated cells was then compared. Results: Fibroblast RARα expression was not affected by 100 nM Dex. On the other hand, RARα expression was 24% lower in cells treated with 10 nM Dex (p < 0.05). Conclusions: Orbital fibroblasts from a GO patient expressed the RARα gene, which was unaffected by higher, but decreased with lower doses of glucocorticoid.
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spelling Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblastsGene expressionGraves ophthalmopathyReceptorsRetinoic acidObjective: Graves’ ophthalmopathy (GO) is an autoimmune disease that leads to ocular proptosis caused by fat accumulation and inflammation, and the main treatment is corticosteroid therapy. Retinoid acid receptor-alpha (RARα) seems to be associated with inflammation and adipocyte differentiation. This study aimed to assess the effect of glucocorticoid treatment on orbital fibroblasts of GO patient treated or not with different glucocorticoid doses. Materials and methods: Orbital fibroblasts collected during orbital decompression of a female patient with moderately severe/severe GO were cultivated and treated with 10 nM and 100 nM dexamethasone (Dex). RARα gene expression in the treated and untreated cells was then compared. Results: Fibroblast RARα expression was not affected by 100 nM Dex. On the other hand, RARα expression was 24% lower in cells treated with 10 nM Dex (p < 0.05). Conclusions: Orbital fibroblasts from a GO patient expressed the RARα gene, which was unaffected by higher, but decreased with lower doses of glucocorticoid.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Divisão de Endocrinologia Departamento de Medicina Interna Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp)Instituto Federal de Educação Ciência e Tecnologia do Estado de São Paulo (IFSP)Departamento de Oftalmologia Otorrinolaringologia e Cirurgia de Cabeça e Pescoço Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp)Divisão de Endocrinologia Departamento de Medicina Interna Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp)Departamento de Oftalmologia Otorrinolaringologia e Cirurgia de Cabeça e Pescoço Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp)FAPESP: #:03/03651-0FAPESP: 2011/18464-8Universidade Estadual Paulista (Unesp)Ciência e Tecnologia do Estado de São Paulo (IFSP)Cury, Sarah Santiloni [UNESP]Oliveira, Miriane [UNESP]Síbio, Maria Teresa [UNESP]Clara, Sueli [UNESP]Luvizotto, Renata De Azevedo Melo [UNESP]Conde, SandroJorge, Edson Nacib [UNESP]Nunes, Vania Dos Santos [UNESP]Nogueira, Célia Regina [UNESP]Mazeto, Gláucia Maria Ferreira da Silva [UNESP]2018-12-11T17:21:56Z2018-12-11T17:21:56Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article366-369application/pdfhttp://dx.doi.org/10.20945/2359-3997000000044Archives of Endocrinology and Metabolism, v. 62, n. 3, p. 366-369, 2018.2359-42922359-3997http://hdl.handle.net/11449/17665310.20945/2359-3997000000044S2359-399720180003003662-s2.0-85050760688S2359-39972018000300366.pdf66922279033003800000-0003-3121-440XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Endocrinology and Metabolism0,5050,505info:eu-repo/semantics/openAccess2024-08-16T18:43:35Zoai:repositorio.unesp.br:11449/176653Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T18:43:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts
title Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts
spellingShingle Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts
Cury, Sarah Santiloni [UNESP]
Gene expression
Graves ophthalmopathy
Receptors
Retinoic acid
title_short Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts
title_full Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts
title_fullStr Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts
title_full_unstemmed Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts
title_sort Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts
author Cury, Sarah Santiloni [UNESP]
author_facet Cury, Sarah Santiloni [UNESP]
Oliveira, Miriane [UNESP]
Síbio, Maria Teresa [UNESP]
Clara, Sueli [UNESP]
Luvizotto, Renata De Azevedo Melo [UNESP]
Conde, Sandro
Jorge, Edson Nacib [UNESP]
Nunes, Vania Dos Santos [UNESP]
Nogueira, Célia Regina [UNESP]
Mazeto, Gláucia Maria Ferreira da Silva [UNESP]
author_role author
author2 Oliveira, Miriane [UNESP]
Síbio, Maria Teresa [UNESP]
Clara, Sueli [UNESP]
Luvizotto, Renata De Azevedo Melo [UNESP]
Conde, Sandro
Jorge, Edson Nacib [UNESP]
Nunes, Vania Dos Santos [UNESP]
Nogueira, Célia Regina [UNESP]
Mazeto, Gláucia Maria Ferreira da Silva [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Ciência e Tecnologia do Estado de São Paulo (IFSP)
dc.contributor.author.fl_str_mv Cury, Sarah Santiloni [UNESP]
Oliveira, Miriane [UNESP]
Síbio, Maria Teresa [UNESP]
Clara, Sueli [UNESP]
Luvizotto, Renata De Azevedo Melo [UNESP]
Conde, Sandro
Jorge, Edson Nacib [UNESP]
Nunes, Vania Dos Santos [UNESP]
Nogueira, Célia Regina [UNESP]
Mazeto, Gláucia Maria Ferreira da Silva [UNESP]
dc.subject.por.fl_str_mv Gene expression
Graves ophthalmopathy
Receptors
Retinoic acid
topic Gene expression
Graves ophthalmopathy
Receptors
Retinoic acid
description Objective: Graves’ ophthalmopathy (GO) is an autoimmune disease that leads to ocular proptosis caused by fat accumulation and inflammation, and the main treatment is corticosteroid therapy. Retinoid acid receptor-alpha (RARα) seems to be associated with inflammation and adipocyte differentiation. This study aimed to assess the effect of glucocorticoid treatment on orbital fibroblasts of GO patient treated or not with different glucocorticoid doses. Materials and methods: Orbital fibroblasts collected during orbital decompression of a female patient with moderately severe/severe GO were cultivated and treated with 10 nM and 100 nM dexamethasone (Dex). RARα gene expression in the treated and untreated cells was then compared. Results: Fibroblast RARα expression was not affected by 100 nM Dex. On the other hand, RARα expression was 24% lower in cells treated with 10 nM Dex (p < 0.05). Conclusions: Orbital fibroblasts from a GO patient expressed the RARα gene, which was unaffected by higher, but decreased with lower doses of glucocorticoid.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:21:56Z
2018-12-11T17:21:56Z
2018-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.20945/2359-3997000000044
Archives of Endocrinology and Metabolism, v. 62, n. 3, p. 366-369, 2018.
2359-4292
2359-3997
http://hdl.handle.net/11449/176653
10.20945/2359-3997000000044
S2359-39972018000300366
2-s2.0-85050760688
S2359-39972018000300366.pdf
6692227903300380
0000-0003-3121-440X
url http://dx.doi.org/10.20945/2359-3997000000044
http://hdl.handle.net/11449/176653
identifier_str_mv Archives of Endocrinology and Metabolism, v. 62, n. 3, p. 366-369, 2018.
2359-4292
2359-3997
10.20945/2359-3997000000044
S2359-39972018000300366
2-s2.0-85050760688
S2359-39972018000300366.pdf
6692227903300380
0000-0003-3121-440X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Endocrinology and Metabolism
0,505
0,505
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 366-369
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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