Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.20945/2359-3997000000044 http://hdl.handle.net/11449/176653 |
Resumo: | Objective: Graves’ ophthalmopathy (GO) is an autoimmune disease that leads to ocular proptosis caused by fat accumulation and inflammation, and the main treatment is corticosteroid therapy. Retinoid acid receptor-alpha (RARα) seems to be associated with inflammation and adipocyte differentiation. This study aimed to assess the effect of glucocorticoid treatment on orbital fibroblasts of GO patient treated or not with different glucocorticoid doses. Materials and methods: Orbital fibroblasts collected during orbital decompression of a female patient with moderately severe/severe GO were cultivated and treated with 10 nM and 100 nM dexamethasone (Dex). RARα gene expression in the treated and untreated cells was then compared. Results: Fibroblast RARα expression was not affected by 100 nM Dex. On the other hand, RARα expression was 24% lower in cells treated with 10 nM Dex (p < 0.05). Conclusions: Orbital fibroblasts from a GO patient expressed the RARα gene, which was unaffected by higher, but decreased with lower doses of glucocorticoid. |
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Repositório Institucional da UNESP |
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spelling |
Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblastsGene expressionGraves ophthalmopathyReceptorsRetinoic acidObjective: Graves’ ophthalmopathy (GO) is an autoimmune disease that leads to ocular proptosis caused by fat accumulation and inflammation, and the main treatment is corticosteroid therapy. Retinoid acid receptor-alpha (RARα) seems to be associated with inflammation and adipocyte differentiation. This study aimed to assess the effect of glucocorticoid treatment on orbital fibroblasts of GO patient treated or not with different glucocorticoid doses. Materials and methods: Orbital fibroblasts collected during orbital decompression of a female patient with moderately severe/severe GO were cultivated and treated with 10 nM and 100 nM dexamethasone (Dex). RARα gene expression in the treated and untreated cells was then compared. Results: Fibroblast RARα expression was not affected by 100 nM Dex. On the other hand, RARα expression was 24% lower in cells treated with 10 nM Dex (p < 0.05). Conclusions: Orbital fibroblasts from a GO patient expressed the RARα gene, which was unaffected by higher, but decreased with lower doses of glucocorticoid.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Divisão de Endocrinologia Departamento de Medicina Interna Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp)Instituto Federal de Educação Ciência e Tecnologia do Estado de São Paulo (IFSP)Departamento de Oftalmologia Otorrinolaringologia e Cirurgia de Cabeça e Pescoço Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp)Divisão de Endocrinologia Departamento de Medicina Interna Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp)Departamento de Oftalmologia Otorrinolaringologia e Cirurgia de Cabeça e Pescoço Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp)FAPESP: #:03/03651-0FAPESP: 2011/18464-8Universidade Estadual Paulista (Unesp)Ciência e Tecnologia do Estado de São Paulo (IFSP)Cury, Sarah Santiloni [UNESP]Oliveira, Miriane [UNESP]Síbio, Maria Teresa [UNESP]Clara, Sueli [UNESP]Luvizotto, Renata De Azevedo Melo [UNESP]Conde, SandroJorge, Edson Nacib [UNESP]Nunes, Vania Dos Santos [UNESP]Nogueira, Célia Regina [UNESP]Mazeto, Gláucia Maria Ferreira da Silva [UNESP]2018-12-11T17:21:56Z2018-12-11T17:21:56Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article366-369application/pdfhttp://dx.doi.org/10.20945/2359-3997000000044Archives of Endocrinology and Metabolism, v. 62, n. 3, p. 366-369, 2018.2359-42922359-3997http://hdl.handle.net/11449/17665310.20945/2359-3997000000044S2359-399720180003003662-s2.0-85050760688S2359-39972018000300366.pdf66922279033003800000-0003-3121-440XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Endocrinology and Metabolism0,5050,505info:eu-repo/semantics/openAccess2024-08-16T18:43:35Zoai:repositorio.unesp.br:11449/176653Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T18:43:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts |
title |
Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts |
spellingShingle |
Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts Cury, Sarah Santiloni [UNESP] Gene expression Graves ophthalmopathy Receptors Retinoic acid |
title_short |
Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts |
title_full |
Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts |
title_fullStr |
Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts |
title_full_unstemmed |
Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts |
title_sort |
Graves’ ophthalmopathy: Low-dose dexamethasone reduces retinoic acid receptor-alpha gene expression in orbital fibroblasts |
author |
Cury, Sarah Santiloni [UNESP] |
author_facet |
Cury, Sarah Santiloni [UNESP] Oliveira, Miriane [UNESP] Síbio, Maria Teresa [UNESP] Clara, Sueli [UNESP] Luvizotto, Renata De Azevedo Melo [UNESP] Conde, Sandro Jorge, Edson Nacib [UNESP] Nunes, Vania Dos Santos [UNESP] Nogueira, Célia Regina [UNESP] Mazeto, Gláucia Maria Ferreira da Silva [UNESP] |
author_role |
author |
author2 |
Oliveira, Miriane [UNESP] Síbio, Maria Teresa [UNESP] Clara, Sueli [UNESP] Luvizotto, Renata De Azevedo Melo [UNESP] Conde, Sandro Jorge, Edson Nacib [UNESP] Nunes, Vania Dos Santos [UNESP] Nogueira, Célia Regina [UNESP] Mazeto, Gláucia Maria Ferreira da Silva [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Ciência e Tecnologia do Estado de São Paulo (IFSP) |
dc.contributor.author.fl_str_mv |
Cury, Sarah Santiloni [UNESP] Oliveira, Miriane [UNESP] Síbio, Maria Teresa [UNESP] Clara, Sueli [UNESP] Luvizotto, Renata De Azevedo Melo [UNESP] Conde, Sandro Jorge, Edson Nacib [UNESP] Nunes, Vania Dos Santos [UNESP] Nogueira, Célia Regina [UNESP] Mazeto, Gláucia Maria Ferreira da Silva [UNESP] |
dc.subject.por.fl_str_mv |
Gene expression Graves ophthalmopathy Receptors Retinoic acid |
topic |
Gene expression Graves ophthalmopathy Receptors Retinoic acid |
description |
Objective: Graves’ ophthalmopathy (GO) is an autoimmune disease that leads to ocular proptosis caused by fat accumulation and inflammation, and the main treatment is corticosteroid therapy. Retinoid acid receptor-alpha (RARα) seems to be associated with inflammation and adipocyte differentiation. This study aimed to assess the effect of glucocorticoid treatment on orbital fibroblasts of GO patient treated or not with different glucocorticoid doses. Materials and methods: Orbital fibroblasts collected during orbital decompression of a female patient with moderately severe/severe GO were cultivated and treated with 10 nM and 100 nM dexamethasone (Dex). RARα gene expression in the treated and untreated cells was then compared. Results: Fibroblast RARα expression was not affected by 100 nM Dex. On the other hand, RARα expression was 24% lower in cells treated with 10 nM Dex (p < 0.05). Conclusions: Orbital fibroblasts from a GO patient expressed the RARα gene, which was unaffected by higher, but decreased with lower doses of glucocorticoid. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:21:56Z 2018-12-11T17:21:56Z 2018-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.20945/2359-3997000000044 Archives of Endocrinology and Metabolism, v. 62, n. 3, p. 366-369, 2018. 2359-4292 2359-3997 http://hdl.handle.net/11449/176653 10.20945/2359-3997000000044 S2359-39972018000300366 2-s2.0-85050760688 S2359-39972018000300366.pdf 6692227903300380 0000-0003-3121-440X |
url |
http://dx.doi.org/10.20945/2359-3997000000044 http://hdl.handle.net/11449/176653 |
identifier_str_mv |
Archives of Endocrinology and Metabolism, v. 62, n. 3, p. 366-369, 2018. 2359-4292 2359-3997 10.20945/2359-3997000000044 S2359-39972018000300366 2-s2.0-85050760688 S2359-39972018000300366.pdf 6692227903300380 0000-0003-3121-440X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Endocrinology and Metabolism 0,505 0,505 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
366-369 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128114479333376 |