Cross species amplification and characterization of microsatellite markers in leporinus friderici (Characiformes, anostomidae)

Detalhes bibliográficos
Autor(a) principal: Utsunomia, Ricardo
Data de Publicação: 2021
Outros Autores: Foresti, Fausto [UNESP], Porto-Foresti, Fábio [UNESP], da Silva, Caio Felipe [UNESP], Martins, Diego Galetti [UNESP], Prado, Fernanda Dotti Do [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0103-8478cr20200072
http://hdl.handle.net/11449/208263
Resumo: Leporinus friderici is a migratory neotropical fish with elevated ecological and economic importance in Brazil. Microsatellite markers are highly important in population genetic studies, management, and conservation programs; however, no markers are available for this species. In this study, seven microsatellite loci, previously developed for Megaleporinus obtusidens, were successfully cross-amplified in L. friderici. Among these loci, five presented moderate to high genetic variability levels, with four to seven alleles per loci and expected heterozygosities varying from ≥ 0.574 to 1.000. These markers represent a valuable tool for the future management and ecological studies involving this species and group of neotropical fishes.
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spelling Cross species amplification and characterization of microsatellite markers in leporinus friderici (Characiformes, anostomidae)Amplificação cruzada e caracterização de marcadores microssatélites em leporinus friderici (Characiformes, anostomidae)Fish geneticsGenetic conservationMolecular markersNeotropical fishLeporinus friderici is a migratory neotropical fish with elevated ecological and economic importance in Brazil. Microsatellite markers are highly important in population genetic studies, management, and conservation programs; however, no markers are available for this species. In this study, seven microsatellite loci, previously developed for Megaleporinus obtusidens, were successfully cross-amplified in L. friderici. Among these loci, five presented moderate to high genetic variability levels, with four to seven alleles per loci and expected heterozygosities varying from ≥ 0.574 to 1.000. These markers represent a valuable tool for the future management and ecological studies involving this species and group of neotropical fishes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)China Three Gorges CorporationFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Faculdade de Ciências Universidade Estadual Paulista (UNESP)Instituto de Biociências Universidade Estadual Paulista (UNESP)Departamento de Genética Instituto de Ciências Biológicas e Saúde (ICBS) Universidade Federal Rural do Rio de Janeiro (UFRRJ)Faculdade de Ciências Universidade Estadual Paulista (UNESP)Instituto de Biociências Universidade Estadual Paulista (UNESP)Universidade Estadual Paulista (Unesp)Universidade Federal Rural do Rio de Janeiro (UFRRJ)Utsunomia, RicardoForesti, Fausto [UNESP]Porto-Foresti, Fábio [UNESP]da Silva, Caio Felipe [UNESP]Martins, Diego Galetti [UNESP]Prado, Fernanda Dotti Do [UNESP]2021-06-25T11:09:18Z2021-06-25T11:09:18Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-4application/pdfhttp://dx.doi.org/10.1590/0103-8478cr20200072Ciencia Rural, v. 51, n. 1, p. 1-4, 2021.1678-45960103-8478http://hdl.handle.net/11449/20826310.1590/0103-8478cr20200072S0103-847820210001004032-s2.0-85097909964S0103-84782021000100403.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCiencia Ruralinfo:eu-repo/semantics/openAccess2024-04-23T15:23:50Zoai:repositorio.unesp.br:11449/208263Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:21:02.751232Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cross species amplification and characterization of microsatellite markers in leporinus friderici (Characiformes, anostomidae)
Amplificação cruzada e caracterização de marcadores microssatélites em leporinus friderici (Characiformes, anostomidae)
title Cross species amplification and characterization of microsatellite markers in leporinus friderici (Characiformes, anostomidae)
spellingShingle Cross species amplification and characterization of microsatellite markers in leporinus friderici (Characiformes, anostomidae)
Utsunomia, Ricardo
Fish genetics
Genetic conservation
Molecular markers
Neotropical fish
title_short Cross species amplification and characterization of microsatellite markers in leporinus friderici (Characiformes, anostomidae)
title_full Cross species amplification and characterization of microsatellite markers in leporinus friderici (Characiformes, anostomidae)
title_fullStr Cross species amplification and characterization of microsatellite markers in leporinus friderici (Characiformes, anostomidae)
title_full_unstemmed Cross species amplification and characterization of microsatellite markers in leporinus friderici (Characiformes, anostomidae)
title_sort Cross species amplification and characterization of microsatellite markers in leporinus friderici (Characiformes, anostomidae)
author Utsunomia, Ricardo
author_facet Utsunomia, Ricardo
Foresti, Fausto [UNESP]
Porto-Foresti, Fábio [UNESP]
da Silva, Caio Felipe [UNESP]
Martins, Diego Galetti [UNESP]
Prado, Fernanda Dotti Do [UNESP]
author_role author
author2 Foresti, Fausto [UNESP]
Porto-Foresti, Fábio [UNESP]
da Silva, Caio Felipe [UNESP]
Martins, Diego Galetti [UNESP]
Prado, Fernanda Dotti Do [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal Rural do Rio de Janeiro (UFRRJ)
dc.contributor.author.fl_str_mv Utsunomia, Ricardo
Foresti, Fausto [UNESP]
Porto-Foresti, Fábio [UNESP]
da Silva, Caio Felipe [UNESP]
Martins, Diego Galetti [UNESP]
Prado, Fernanda Dotti Do [UNESP]
dc.subject.por.fl_str_mv Fish genetics
Genetic conservation
Molecular markers
Neotropical fish
topic Fish genetics
Genetic conservation
Molecular markers
Neotropical fish
description Leporinus friderici is a migratory neotropical fish with elevated ecological and economic importance in Brazil. Microsatellite markers are highly important in population genetic studies, management, and conservation programs; however, no markers are available for this species. In this study, seven microsatellite loci, previously developed for Megaleporinus obtusidens, were successfully cross-amplified in L. friderici. Among these loci, five presented moderate to high genetic variability levels, with four to seven alleles per loci and expected heterozygosities varying from ≥ 0.574 to 1.000. These markers represent a valuable tool for the future management and ecological studies involving this species and group of neotropical fishes.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T11:09:18Z
2021-06-25T11:09:18Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0103-8478cr20200072
Ciencia Rural, v. 51, n. 1, p. 1-4, 2021.
1678-4596
0103-8478
http://hdl.handle.net/11449/208263
10.1590/0103-8478cr20200072
S0103-84782021000100403
2-s2.0-85097909964
S0103-84782021000100403.pdf
url http://dx.doi.org/10.1590/0103-8478cr20200072
http://hdl.handle.net/11449/208263
identifier_str_mv Ciencia Rural, v. 51, n. 1, p. 1-4, 2021.
1678-4596
0103-8478
10.1590/0103-8478cr20200072
S0103-84782021000100403
2-s2.0-85097909964
S0103-84782021000100403.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ciencia Rural
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-4
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129418964500480

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