Transmission Electron Microscopy of the Presbylarynx in the Process of Voice Aging
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jvoice.2016.11.013 http://hdl.handle.net/11449/175317 |
Resumo: | Introduction: Microscopy studies of presbylarynx have identified epithelial atrophy, as well as vocal muscle atrophy, increased collagen, and decreased nonfibrous proteins of the extracellular matrix. Studies on the ultrastructure of presbylarynx are rare and can help us understand the pathophysiology of presbyphonia. Objective: To describe details on the ultrastructure of presbylarynx by transmission electron microscopy (TEM). Subjects and Methods: Sixteen human larynges were removed and distributed in two groups: control (n = 8; 30–50 years; six females, two males) and seniors (n = 8; 75–92 years; six females, two males). After preparation for TEM, the specimens, including epithelium and lamina propria, were examined and photographed at increasing magnification. Results: Control: the epithelium was intact, with overlapped cells, and with desmosomes between the intercellular junctions. The basement membrane was continuous and uniform, and the lamina propria contained collagen and elastic fibers in a regularly distributed loose arrangement, as well as some fibroblasts of different shapes. Senior: the epithelial cells were separated by enlarged intercellular junctions. The basement membrane was delicate and continuous. In the lamina propria there was predominance of elongated fibroblasts. Under the basement membrane a dense network of collagen fibers could be identified. Conclusions: In this study with TEM some structural changes were identified, particularly in the larynx of the elderly, both in the epithelium and lamina propria, some of them with likely participation of fibroblasts, which reinforces the importance of additional ultrastructure as well as molecular studies targeting those cells, as they are the main precursors of the components of the extracellular matrix. |
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Transmission Electron Microscopy of the Presbylarynx in the Process of Voice AgingAging voiceElderlyPresbylarynxTransmission electron microscopyUltrastructureIntroduction: Microscopy studies of presbylarynx have identified epithelial atrophy, as well as vocal muscle atrophy, increased collagen, and decreased nonfibrous proteins of the extracellular matrix. Studies on the ultrastructure of presbylarynx are rare and can help us understand the pathophysiology of presbyphonia. Objective: To describe details on the ultrastructure of presbylarynx by transmission electron microscopy (TEM). Subjects and Methods: Sixteen human larynges were removed and distributed in two groups: control (n = 8; 30–50 years; six females, two males) and seniors (n = 8; 75–92 years; six females, two males). After preparation for TEM, the specimens, including epithelium and lamina propria, were examined and photographed at increasing magnification. Results: Control: the epithelium was intact, with overlapped cells, and with desmosomes between the intercellular junctions. The basement membrane was continuous and uniform, and the lamina propria contained collagen and elastic fibers in a regularly distributed loose arrangement, as well as some fibroblasts of different shapes. Senior: the epithelial cells were separated by enlarged intercellular junctions. The basement membrane was delicate and continuous. In the lamina propria there was predominance of elongated fibroblasts. Under the basement membrane a dense network of collagen fibers could be identified. Conclusions: In this study with TEM some structural changes were identified, particularly in the larynx of the elderly, both in the epithelium and lamina propria, some of them with likely participation of fibroblasts, which reinforces the importance of additional ultrastructure as well as molecular studies targeting those cells, as they are the main precursors of the components of the extracellular matrix.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Ophthalmology Otorhinolaryngology and Head Neck Surgery Botucatu Medical School Universidade Estadual Paulista (UNESP)Department of Ophthalmology Otorhinolaryngology and Head Neck Surgery Botucatu Medical School Universidade Estadual Paulista (UNESP)FAPESP: 2014/13741-1Universidade Estadual Paulista (Unesp)Gonçalves, Tatiana Maria [UNESP]Martins, Regina Helena Garcia [UNESP]Adriana, Bueno Benito Pessin [UNESP]2018-12-11T17:15:16Z2018-12-11T17:15:16Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3-7application/pdfhttp://dx.doi.org/10.1016/j.jvoice.2016.11.013Journal of Voice, v. 32, n. 1, p. 3-7, 2018.1873-45880892-1997http://hdl.handle.net/11449/17531710.1016/j.jvoice.2016.11.0132-s2.0-850308444622-s2.0-85030844462.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Voice0,735info:eu-repo/semantics/openAccess2024-08-16T18:44:04Zoai:repositorio.unesp.br:11449/175317Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T18:44:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Transmission Electron Microscopy of the Presbylarynx in the Process of Voice Aging |
title |
Transmission Electron Microscopy of the Presbylarynx in the Process of Voice Aging |
spellingShingle |
Transmission Electron Microscopy of the Presbylarynx in the Process of Voice Aging Gonçalves, Tatiana Maria [UNESP] Aging voice Elderly Presbylarynx Transmission electron microscopy Ultrastructure |
title_short |
Transmission Electron Microscopy of the Presbylarynx in the Process of Voice Aging |
title_full |
Transmission Electron Microscopy of the Presbylarynx in the Process of Voice Aging |
title_fullStr |
Transmission Electron Microscopy of the Presbylarynx in the Process of Voice Aging |
title_full_unstemmed |
Transmission Electron Microscopy of the Presbylarynx in the Process of Voice Aging |
title_sort |
Transmission Electron Microscopy of the Presbylarynx in the Process of Voice Aging |
author |
Gonçalves, Tatiana Maria [UNESP] |
author_facet |
Gonçalves, Tatiana Maria [UNESP] Martins, Regina Helena Garcia [UNESP] Adriana, Bueno Benito Pessin [UNESP] |
author_role |
author |
author2 |
Martins, Regina Helena Garcia [UNESP] Adriana, Bueno Benito Pessin [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Gonçalves, Tatiana Maria [UNESP] Martins, Regina Helena Garcia [UNESP] Adriana, Bueno Benito Pessin [UNESP] |
dc.subject.por.fl_str_mv |
Aging voice Elderly Presbylarynx Transmission electron microscopy Ultrastructure |
topic |
Aging voice Elderly Presbylarynx Transmission electron microscopy Ultrastructure |
description |
Introduction: Microscopy studies of presbylarynx have identified epithelial atrophy, as well as vocal muscle atrophy, increased collagen, and decreased nonfibrous proteins of the extracellular matrix. Studies on the ultrastructure of presbylarynx are rare and can help us understand the pathophysiology of presbyphonia. Objective: To describe details on the ultrastructure of presbylarynx by transmission electron microscopy (TEM). Subjects and Methods: Sixteen human larynges were removed and distributed in two groups: control (n = 8; 30–50 years; six females, two males) and seniors (n = 8; 75–92 years; six females, two males). After preparation for TEM, the specimens, including epithelium and lamina propria, were examined and photographed at increasing magnification. Results: Control: the epithelium was intact, with overlapped cells, and with desmosomes between the intercellular junctions. The basement membrane was continuous and uniform, and the lamina propria contained collagen and elastic fibers in a regularly distributed loose arrangement, as well as some fibroblasts of different shapes. Senior: the epithelial cells were separated by enlarged intercellular junctions. The basement membrane was delicate and continuous. In the lamina propria there was predominance of elongated fibroblasts. Under the basement membrane a dense network of collagen fibers could be identified. Conclusions: In this study with TEM some structural changes were identified, particularly in the larynx of the elderly, both in the epithelium and lamina propria, some of them with likely participation of fibroblasts, which reinforces the importance of additional ultrastructure as well as molecular studies targeting those cells, as they are the main precursors of the components of the extracellular matrix. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:15:16Z 2018-12-11T17:15:16Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jvoice.2016.11.013 Journal of Voice, v. 32, n. 1, p. 3-7, 2018. 1873-4588 0892-1997 http://hdl.handle.net/11449/175317 10.1016/j.jvoice.2016.11.013 2-s2.0-85030844462 2-s2.0-85030844462.pdf |
url |
http://dx.doi.org/10.1016/j.jvoice.2016.11.013 http://hdl.handle.net/11449/175317 |
identifier_str_mv |
Journal of Voice, v. 32, n. 1, p. 3-7, 2018. 1873-4588 0892-1997 10.1016/j.jvoice.2016.11.013 2-s2.0-85030844462 2-s2.0-85030844462.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Voice 0,735 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3-7 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128145163812864 |