CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis

Detalhes bibliográficos
Autor(a) principal: de Faria Junior, Geraldo M.
Data de Publicação: 2018
Outros Autores: Ayo, Christiane M., de Oliveira, Amanda P., Lopes, Alessandro G. [UNESP], Frederico, Fábio B., Silveira-Carvalho, Aparecida P., Previato, Mariana, Barbosa, Amanda P., Murata, Fernando H.A., de Almeida Junior, Gildásio Castello, Siqueira, Rubens Camargo, de Mattos, Luiz C., Brandão de Mattos, Cinara C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.actatropica.2017.12.012
http://hdl.handle.net/11449/175611
Resumo: C–C chemokine receptor type 5 (CCR5) is a chemokine receptor that influences the immune response to infectious and parasitic diseases. This study aimed to determine whether the CCR5Δ32 and CCR5 59029 A/G polymorphisms are associated with the development of ocular toxoplasmosis in humans. Patients with positive serology for Toxoplasma gondii were analyzed and grouped as ‘with ocular toxoplasmosis’ (G1: n = 160) or ‘without ocular toxoplasmosis’ (G2: n = 160). A control group (G3) consisted of 160 individuals with negative serology. The characterization of the CCR5Δ32 and CCR5 59029 A/G polymorphisms was by PCR and by PCR-RFLP, respectively. The difference between the groups with respect to the mean age (G1: mean age: 47.3, SD ± 19.3, median: 46 [range: 18–95]; G2: mean age: 61.3, SD ± 13.7, median: 61 [range: 21–87]; G3: mean age: 38.8, SD ± 17.9, median: 34 [range: 18–80]) was statistically significant (G1 vs.G2: p-value <0.0001; t = 7.21; DF = 318; G1 vs.G3: p-value <0.0001; t = 4.32; DF = 318; G2 vs. G3: p-value <0.0001; t = 9.62; DF = 318). The Nagelkerke r2 value was 0.040. There were statistically significant differences for the CCR5/CCR5 (p-value = 0.008; OR = 0.261), AA (p-value = 0.007; OR = 2.974) and AG genotypes (p-value = 0.018; OR = 2.447) between G1 and G2. Individuals with the CCR5/CCR5 genotype and simultaneously the CCR5-59029 AA or AG genotypes have a greater risk of developing ocular toxoplasmosis (4% greater), which may be associated with a strong and persistent inflammatory response in ocular tissue.
id UNSP_a2ce7f67aece2e71c55a5047c1e48e26
oai_identifier_str oai:repositorio.unesp.br:11449/175611
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosisCCR5 receptorChemokinesGenetic polymorphismOcular toxoplasmosisToxoplasma gondiiC–C chemokine receptor type 5 (CCR5) is a chemokine receptor that influences the immune response to infectious and parasitic diseases. This study aimed to determine whether the CCR5Δ32 and CCR5 59029 A/G polymorphisms are associated with the development of ocular toxoplasmosis in humans. Patients with positive serology for Toxoplasma gondii were analyzed and grouped as ‘with ocular toxoplasmosis’ (G1: n = 160) or ‘without ocular toxoplasmosis’ (G2: n = 160). A control group (G3) consisted of 160 individuals with negative serology. The characterization of the CCR5Δ32 and CCR5 59029 A/G polymorphisms was by PCR and by PCR-RFLP, respectively. The difference between the groups with respect to the mean age (G1: mean age: 47.3, SD ± 19.3, median: 46 [range: 18–95]; G2: mean age: 61.3, SD ± 13.7, median: 61 [range: 21–87]; G3: mean age: 38.8, SD ± 17.9, median: 34 [range: 18–80]) was statistically significant (G1 vs.G2: p-value <0.0001; t = 7.21; DF = 318; G1 vs.G3: p-value <0.0001; t = 4.32; DF = 318; G2 vs. G3: p-value <0.0001; t = 9.62; DF = 318). The Nagelkerke r2 value was 0.040. There were statistically significant differences for the CCR5/CCR5 (p-value = 0.008; OR = 0.261), AA (p-value = 0.007; OR = 2.974) and AG genotypes (p-value = 0.018; OR = 2.447) between G1 and G2. Individuals with the CCR5/CCR5 genotype and simultaneously the CCR5-59029 AA or AG genotypes have a greater risk of developing ocular toxoplasmosis (4% greater), which may be associated with a strong and persistent inflammatory response in ocular tissue.Immunogenetics Laboratory Molecular Biology Department Faculdade de Medicina de São José do Rio Preto (FAMERP)Ophthalmology Outpatient Clinic of Hospital de Base da Fundação Faculdade Regional de Medicina de São José do Rio Preto (HB– FUNFARME)Toxoplasma Research Group Faculdade de Medicina de São José do Rio Preto (FAMERP)Biology Department Instituto de Biociências Letras e Ciências Exatas (IBILCE) Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)Medical Specialties Outpatient Clinic of Hospital Estadual “João Paulo II” (AME)Biology Department Instituto de Biociências Letras e Ciências Exatas (IBILCE) Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)Faculdade de Medicina de São José do Rio Preto (FAMERP)Ophthalmology Outpatient Clinic of Hospital de Base da Fundação Faculdade Regional de Medicina de São José do Rio Preto (HB– FUNFARME)Universidade Estadual Paulista (Unesp)Medical Specialties Outpatient Clinic of Hospital Estadual “João Paulo II” (AME)de Faria Junior, Geraldo M.Ayo, Christiane M.de Oliveira, Amanda P.Lopes, Alessandro G. [UNESP]Frederico, Fábio B.Silveira-Carvalho, Aparecida P.Previato, MarianaBarbosa, Amanda P.Murata, Fernando H.A.de Almeida Junior, Gildásio CastelloSiqueira, Rubens Camargode Mattos, Luiz C.Brandão de Mattos, Cinara C.2018-12-11T17:16:42Z2018-12-11T17:16:42Z2018-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article276-280application/pdfhttp://dx.doi.org/10.1016/j.actatropica.2017.12.012Acta Tropica, v. 178, p. 276-280.1873-62540001-706Xhttp://hdl.handle.net/11449/17561110.1016/j.actatropica.2017.12.0122-s2.0-850376458932-s2.0-85037645893.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Tropicainfo:eu-repo/semantics/openAccess2023-11-20T06:09:37Zoai:repositorio.unesp.br:11449/175611Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:10:46.819978Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis
title CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis
spellingShingle CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis
de Faria Junior, Geraldo M.
CCR5 receptor
Chemokines
Genetic polymorphism
Ocular toxoplasmosis
Toxoplasma gondii
title_short CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis
title_full CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis
title_fullStr CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis
title_full_unstemmed CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis
title_sort CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis
author de Faria Junior, Geraldo M.
author_facet de Faria Junior, Geraldo M.
Ayo, Christiane M.
de Oliveira, Amanda P.
Lopes, Alessandro G. [UNESP]
Frederico, Fábio B.
Silveira-Carvalho, Aparecida P.
Previato, Mariana
Barbosa, Amanda P.
Murata, Fernando H.A.
de Almeida Junior, Gildásio Castello
Siqueira, Rubens Camargo
de Mattos, Luiz C.
Brandão de Mattos, Cinara C.
author_role author
author2 Ayo, Christiane M.
de Oliveira, Amanda P.
Lopes, Alessandro G. [UNESP]
Frederico, Fábio B.
Silveira-Carvalho, Aparecida P.
Previato, Mariana
Barbosa, Amanda P.
Murata, Fernando H.A.
de Almeida Junior, Gildásio Castello
Siqueira, Rubens Camargo
de Mattos, Luiz C.
Brandão de Mattos, Cinara C.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Faculdade de Medicina de São José do Rio Preto (FAMERP)
Ophthalmology Outpatient Clinic of Hospital de Base da Fundação Faculdade Regional de Medicina de São José do Rio Preto (HB– FUNFARME)
Universidade Estadual Paulista (Unesp)
Medical Specialties Outpatient Clinic of Hospital Estadual “João Paulo II” (AME)
dc.contributor.author.fl_str_mv de Faria Junior, Geraldo M.
Ayo, Christiane M.
de Oliveira, Amanda P.
Lopes, Alessandro G. [UNESP]
Frederico, Fábio B.
Silveira-Carvalho, Aparecida P.
Previato, Mariana
Barbosa, Amanda P.
Murata, Fernando H.A.
de Almeida Junior, Gildásio Castello
Siqueira, Rubens Camargo
de Mattos, Luiz C.
Brandão de Mattos, Cinara C.
dc.subject.por.fl_str_mv CCR5 receptor
Chemokines
Genetic polymorphism
Ocular toxoplasmosis
Toxoplasma gondii
topic CCR5 receptor
Chemokines
Genetic polymorphism
Ocular toxoplasmosis
Toxoplasma gondii
description C–C chemokine receptor type 5 (CCR5) is a chemokine receptor that influences the immune response to infectious and parasitic diseases. This study aimed to determine whether the CCR5Δ32 and CCR5 59029 A/G polymorphisms are associated with the development of ocular toxoplasmosis in humans. Patients with positive serology for Toxoplasma gondii were analyzed and grouped as ‘with ocular toxoplasmosis’ (G1: n = 160) or ‘without ocular toxoplasmosis’ (G2: n = 160). A control group (G3) consisted of 160 individuals with negative serology. The characterization of the CCR5Δ32 and CCR5 59029 A/G polymorphisms was by PCR and by PCR-RFLP, respectively. The difference between the groups with respect to the mean age (G1: mean age: 47.3, SD ± 19.3, median: 46 [range: 18–95]; G2: mean age: 61.3, SD ± 13.7, median: 61 [range: 21–87]; G3: mean age: 38.8, SD ± 17.9, median: 34 [range: 18–80]) was statistically significant (G1 vs.G2: p-value <0.0001; t = 7.21; DF = 318; G1 vs.G3: p-value <0.0001; t = 4.32; DF = 318; G2 vs. G3: p-value <0.0001; t = 9.62; DF = 318). The Nagelkerke r2 value was 0.040. There were statistically significant differences for the CCR5/CCR5 (p-value = 0.008; OR = 0.261), AA (p-value = 0.007; OR = 2.974) and AG genotypes (p-value = 0.018; OR = 2.447) between G1 and G2. Individuals with the CCR5/CCR5 genotype and simultaneously the CCR5-59029 AA or AG genotypes have a greater risk of developing ocular toxoplasmosis (4% greater), which may be associated with a strong and persistent inflammatory response in ocular tissue.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:16:42Z
2018-12-11T17:16:42Z
2018-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.actatropica.2017.12.012
Acta Tropica, v. 178, p. 276-280.
1873-6254
0001-706X
http://hdl.handle.net/11449/175611
10.1016/j.actatropica.2017.12.012
2-s2.0-85037645893
2-s2.0-85037645893.pdf
url http://dx.doi.org/10.1016/j.actatropica.2017.12.012
http://hdl.handle.net/11449/175611
identifier_str_mv Acta Tropica, v. 178, p. 276-280.
1873-6254
0001-706X
10.1016/j.actatropica.2017.12.012
2-s2.0-85037645893
2-s2.0-85037645893.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Tropica
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 276-280
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128905848029184