CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.actatropica.2017.12.012 http://hdl.handle.net/11449/175611 |
Resumo: | C–C chemokine receptor type 5 (CCR5) is a chemokine receptor that influences the immune response to infectious and parasitic diseases. This study aimed to determine whether the CCR5Δ32 and CCR5 59029 A/G polymorphisms are associated with the development of ocular toxoplasmosis in humans. Patients with positive serology for Toxoplasma gondii were analyzed and grouped as ‘with ocular toxoplasmosis’ (G1: n = 160) or ‘without ocular toxoplasmosis’ (G2: n = 160). A control group (G3) consisted of 160 individuals with negative serology. The characterization of the CCR5Δ32 and CCR5 59029 A/G polymorphisms was by PCR and by PCR-RFLP, respectively. The difference between the groups with respect to the mean age (G1: mean age: 47.3, SD ± 19.3, median: 46 [range: 18–95]; G2: mean age: 61.3, SD ± 13.7, median: 61 [range: 21–87]; G3: mean age: 38.8, SD ± 17.9, median: 34 [range: 18–80]) was statistically significant (G1 vs.G2: p-value <0.0001; t = 7.21; DF = 318; G1 vs.G3: p-value <0.0001; t = 4.32; DF = 318; G2 vs. G3: p-value <0.0001; t = 9.62; DF = 318). The Nagelkerke r2 value was 0.040. There were statistically significant differences for the CCR5/CCR5 (p-value = 0.008; OR = 0.261), AA (p-value = 0.007; OR = 2.974) and AG genotypes (p-value = 0.018; OR = 2.447) between G1 and G2. Individuals with the CCR5/CCR5 genotype and simultaneously the CCR5-59029 AA or AG genotypes have a greater risk of developing ocular toxoplasmosis (4% greater), which may be associated with a strong and persistent inflammatory response in ocular tissue. |
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CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosisCCR5 receptorChemokinesGenetic polymorphismOcular toxoplasmosisToxoplasma gondiiC–C chemokine receptor type 5 (CCR5) is a chemokine receptor that influences the immune response to infectious and parasitic diseases. This study aimed to determine whether the CCR5Δ32 and CCR5 59029 A/G polymorphisms are associated with the development of ocular toxoplasmosis in humans. Patients with positive serology for Toxoplasma gondii were analyzed and grouped as ‘with ocular toxoplasmosis’ (G1: n = 160) or ‘without ocular toxoplasmosis’ (G2: n = 160). A control group (G3) consisted of 160 individuals with negative serology. The characterization of the CCR5Δ32 and CCR5 59029 A/G polymorphisms was by PCR and by PCR-RFLP, respectively. The difference between the groups with respect to the mean age (G1: mean age: 47.3, SD ± 19.3, median: 46 [range: 18–95]; G2: mean age: 61.3, SD ± 13.7, median: 61 [range: 21–87]; G3: mean age: 38.8, SD ± 17.9, median: 34 [range: 18–80]) was statistically significant (G1 vs.G2: p-value <0.0001; t = 7.21; DF = 318; G1 vs.G3: p-value <0.0001; t = 4.32; DF = 318; G2 vs. G3: p-value <0.0001; t = 9.62; DF = 318). The Nagelkerke r2 value was 0.040. There were statistically significant differences for the CCR5/CCR5 (p-value = 0.008; OR = 0.261), AA (p-value = 0.007; OR = 2.974) and AG genotypes (p-value = 0.018; OR = 2.447) between G1 and G2. Individuals with the CCR5/CCR5 genotype and simultaneously the CCR5-59029 AA or AG genotypes have a greater risk of developing ocular toxoplasmosis (4% greater), which may be associated with a strong and persistent inflammatory response in ocular tissue.Immunogenetics Laboratory Molecular Biology Department Faculdade de Medicina de São José do Rio Preto (FAMERP)Ophthalmology Outpatient Clinic of Hospital de Base da Fundação Faculdade Regional de Medicina de São José do Rio Preto (HB– FUNFARME)Toxoplasma Research Group Faculdade de Medicina de São José do Rio Preto (FAMERP)Biology Department Instituto de Biociências Letras e Ciências Exatas (IBILCE) Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)Medical Specialties Outpatient Clinic of Hospital Estadual “João Paulo II” (AME)Biology Department Instituto de Biociências Letras e Ciências Exatas (IBILCE) Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)Faculdade de Medicina de São José do Rio Preto (FAMERP)Ophthalmology Outpatient Clinic of Hospital de Base da Fundação Faculdade Regional de Medicina de São José do Rio Preto (HB– FUNFARME)Universidade Estadual Paulista (Unesp)Medical Specialties Outpatient Clinic of Hospital Estadual “João Paulo II” (AME)de Faria Junior, Geraldo M.Ayo, Christiane M.de Oliveira, Amanda P.Lopes, Alessandro G. [UNESP]Frederico, Fábio B.Silveira-Carvalho, Aparecida P.Previato, MarianaBarbosa, Amanda P.Murata, Fernando H.A.de Almeida Junior, Gildásio CastelloSiqueira, Rubens Camargode Mattos, Luiz C.Brandão de Mattos, Cinara C.2018-12-11T17:16:42Z2018-12-11T17:16:42Z2018-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article276-280application/pdfhttp://dx.doi.org/10.1016/j.actatropica.2017.12.012Acta Tropica, v. 178, p. 276-280.1873-62540001-706Xhttp://hdl.handle.net/11449/17561110.1016/j.actatropica.2017.12.0122-s2.0-850376458932-s2.0-85037645893.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Tropicainfo:eu-repo/semantics/openAccess2023-11-20T06:09:37Zoai:repositorio.unesp.br:11449/175611Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:10:46.819978Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis |
title |
CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis |
spellingShingle |
CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis de Faria Junior, Geraldo M. CCR5 receptor Chemokines Genetic polymorphism Ocular toxoplasmosis Toxoplasma gondii |
title_short |
CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis |
title_full |
CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis |
title_fullStr |
CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis |
title_full_unstemmed |
CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis |
title_sort |
CCR5 chemokine receptor gene polymorphisms in ocular toxoplasmosis |
author |
de Faria Junior, Geraldo M. |
author_facet |
de Faria Junior, Geraldo M. Ayo, Christiane M. de Oliveira, Amanda P. Lopes, Alessandro G. [UNESP] Frederico, Fábio B. Silveira-Carvalho, Aparecida P. Previato, Mariana Barbosa, Amanda P. Murata, Fernando H.A. de Almeida Junior, Gildásio Castello Siqueira, Rubens Camargo de Mattos, Luiz C. Brandão de Mattos, Cinara C. |
author_role |
author |
author2 |
Ayo, Christiane M. de Oliveira, Amanda P. Lopes, Alessandro G. [UNESP] Frederico, Fábio B. Silveira-Carvalho, Aparecida P. Previato, Mariana Barbosa, Amanda P. Murata, Fernando H.A. de Almeida Junior, Gildásio Castello Siqueira, Rubens Camargo de Mattos, Luiz C. Brandão de Mattos, Cinara C. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Faculdade de Medicina de São José do Rio Preto (FAMERP) Ophthalmology Outpatient Clinic of Hospital de Base da Fundação Faculdade Regional de Medicina de São José do Rio Preto (HB– FUNFARME) Universidade Estadual Paulista (Unesp) Medical Specialties Outpatient Clinic of Hospital Estadual “João Paulo II” (AME) |
dc.contributor.author.fl_str_mv |
de Faria Junior, Geraldo M. Ayo, Christiane M. de Oliveira, Amanda P. Lopes, Alessandro G. [UNESP] Frederico, Fábio B. Silveira-Carvalho, Aparecida P. Previato, Mariana Barbosa, Amanda P. Murata, Fernando H.A. de Almeida Junior, Gildásio Castello Siqueira, Rubens Camargo de Mattos, Luiz C. Brandão de Mattos, Cinara C. |
dc.subject.por.fl_str_mv |
CCR5 receptor Chemokines Genetic polymorphism Ocular toxoplasmosis Toxoplasma gondii |
topic |
CCR5 receptor Chemokines Genetic polymorphism Ocular toxoplasmosis Toxoplasma gondii |
description |
C–C chemokine receptor type 5 (CCR5) is a chemokine receptor that influences the immune response to infectious and parasitic diseases. This study aimed to determine whether the CCR5Δ32 and CCR5 59029 A/G polymorphisms are associated with the development of ocular toxoplasmosis in humans. Patients with positive serology for Toxoplasma gondii were analyzed and grouped as ‘with ocular toxoplasmosis’ (G1: n = 160) or ‘without ocular toxoplasmosis’ (G2: n = 160). A control group (G3) consisted of 160 individuals with negative serology. The characterization of the CCR5Δ32 and CCR5 59029 A/G polymorphisms was by PCR and by PCR-RFLP, respectively. The difference between the groups with respect to the mean age (G1: mean age: 47.3, SD ± 19.3, median: 46 [range: 18–95]; G2: mean age: 61.3, SD ± 13.7, median: 61 [range: 21–87]; G3: mean age: 38.8, SD ± 17.9, median: 34 [range: 18–80]) was statistically significant (G1 vs.G2: p-value <0.0001; t = 7.21; DF = 318; G1 vs.G3: p-value <0.0001; t = 4.32; DF = 318; G2 vs. G3: p-value <0.0001; t = 9.62; DF = 318). The Nagelkerke r2 value was 0.040. There were statistically significant differences for the CCR5/CCR5 (p-value = 0.008; OR = 0.261), AA (p-value = 0.007; OR = 2.974) and AG genotypes (p-value = 0.018; OR = 2.447) between G1 and G2. Individuals with the CCR5/CCR5 genotype and simultaneously the CCR5-59029 AA or AG genotypes have a greater risk of developing ocular toxoplasmosis (4% greater), which may be associated with a strong and persistent inflammatory response in ocular tissue. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:16:42Z 2018-12-11T17:16:42Z 2018-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.actatropica.2017.12.012 Acta Tropica, v. 178, p. 276-280. 1873-6254 0001-706X http://hdl.handle.net/11449/175611 10.1016/j.actatropica.2017.12.012 2-s2.0-85037645893 2-s2.0-85037645893.pdf |
url |
http://dx.doi.org/10.1016/j.actatropica.2017.12.012 http://hdl.handle.net/11449/175611 |
identifier_str_mv |
Acta Tropica, v. 178, p. 276-280. 1873-6254 0001-706X 10.1016/j.actatropica.2017.12.012 2-s2.0-85037645893 2-s2.0-85037645893.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Tropica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
276-280 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128905848029184 |