Toxicological impact of SARS-CoV-2 on the health of the neotropical fish, Poecilia reticulata

Detalhes bibliográficos
Autor(a) principal: Malafaia, Guilherme
Data de Publicação: 2022
Outros Autores: Ahmed, Mohamed Ahmed Ibrahim, Souza, Sindoval Silva de, Rezende, Fernanda Neves Estrela, Freitas, Ítalo Nascimento, da Luz, Thiarlen Marinho, da Silva, Abner Marcelino, Charlie-Silva, Ives, Braz, Helyson Lucas Bezerra, Jorge, Roberta Jeane Bezerra, Sanches, Paulo R.S. [UNESP], Mendonça-Gomes, Juliana Moreira, Cilli, Eduardo M. [UNESP], Araújo, Amanda Pereira da Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.aquatox.2022.106104
http://hdl.handle.net/11449/231616
Resumo: There have been significant impacts of the current COVID-19 pandemic on society including high health and economic costs. However, little is known about the potential ecological risks of this virus despite its presence in freshwater systems. In this study, we aimed to evaluate the exposure of Poecilia reticulata juveniles to two peptides derived from Spike protein of SARS-CoV-2, which was synthesized in the laboratory (named PSPD-2002 and PSPD-2003). For this, the animals were exposed for 35 days to the peptides at a concentration of 40 µg/L and different toxicity biomarkers were assessed. Our data indicated that the peptides were able to induce anxiety-like behavior in the open field test and increased acetylcholinesterase (AChE) activity. The biometric evaluation also revealed that the animals exposed to the peptides displayed alterations in the pattern of growth/development. Furthermore, the increased activity of superoxide dismutase (SOD) and catalase (CAT) enzymes were accompanied by increased levels of malondialdehyde (MDA), reactive oxygen species (ROS) and hydrogen peroxide (H2O2), which suggests a redox imbalance induced by SARS-CoV-2 spike protein peptides. Moreover, molecular docking analysis suggested a strong interaction of the peptides with the enzymes AChE, SOD and CAT, allowing us to infer that the observed effects are related to the direct action of the peptides on the functionality of these enzymes. Consequently, our study provided evidence that the presence of SARS-CoV-2 viral particles in the freshwater ecosystems offer a health risk to fish and other aquatic organisms.
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spelling Toxicological impact of SARS-CoV-2 on the health of the neotropical fish, Poecilia reticulataBehaviorCOVID-19FishREDOX unbalanceViral particlesWater pollutionThere have been significant impacts of the current COVID-19 pandemic on society including high health and economic costs. However, little is known about the potential ecological risks of this virus despite its presence in freshwater systems. In this study, we aimed to evaluate the exposure of Poecilia reticulata juveniles to two peptides derived from Spike protein of SARS-CoV-2, which was synthesized in the laboratory (named PSPD-2002 and PSPD-2003). For this, the animals were exposed for 35 days to the peptides at a concentration of 40 µg/L and different toxicity biomarkers were assessed. Our data indicated that the peptides were able to induce anxiety-like behavior in the open field test and increased acetylcholinesterase (AChE) activity. The biometric evaluation also revealed that the animals exposed to the peptides displayed alterations in the pattern of growth/development. Furthermore, the increased activity of superoxide dismutase (SOD) and catalase (CAT) enzymes were accompanied by increased levels of malondialdehyde (MDA), reactive oxygen species (ROS) and hydrogen peroxide (H2O2), which suggests a redox imbalance induced by SARS-CoV-2 spike protein peptides. Moreover, molecular docking analysis suggested a strong interaction of the peptides with the enzymes AChE, SOD and CAT, allowing us to infer that the observed effects are related to the direct action of the peptides on the functionality of these enzymes. Consequently, our study provided evidence that the presence of SARS-CoV-2 viral particles in the freshwater ecosystems offer a health risk to fish and other aquatic organisms.Biological Research Laboratory Post-Graduation Program in Conservation of Cerrado Natural Resources Goiano Federal Institute, Urutaí Campus, Rodovia Geraldo Silva Nascimento, 2,5km, Zona Rural CEP, GOPost-Graduation Program in Biotechnology and Biodiversity Goiano Federal Institution and Federal University of Goiás, GOPost-Graduation Program in Ecology and Conservation of Natural Resources Federal University of Uberlândia, MGPlant Protection Department Faculty of Agriculture Assiut UniversityPost-Graduation Programa in Environmental Sciences Federal University of Goiás, GODepartment of Pharmacology Institute of Biomedical Sciences University of Sao Paulo, SPDrug Research and Development Center Federal University of Ceará, CEDepartment of Physiology and Pharmacology School of Medicine Federal University of Ceará, CEInstituto de Química Universidade Estadual Paulista, SPDepartamento de Imunologia Instituto de Ciências Biomédicas Universidade de São Paulo, SPInstituto de Química Universidade Estadual Paulista, SPGoiano Federal InstituteUniversidade Federal de Goiás (UFG)Universidade Federal de Uberlândia (UFU)Assiut UniversityUniversidade de São Paulo (USP)Federal University of CearáUniversidade Estadual Paulista (UNESP)Malafaia, GuilhermeAhmed, Mohamed Ahmed IbrahimSouza, Sindoval Silva deRezende, Fernanda Neves EstrelaFreitas, Ítalo Nascimentoda Luz, Thiarlen Marinhoda Silva, Abner MarcelinoCharlie-Silva, IvesBraz, Helyson Lucas BezerraJorge, Roberta Jeane BezerraSanches, Paulo R.S. [UNESP]Mendonça-Gomes, Juliana MoreiraCilli, Eduardo M. [UNESP]Araújo, Amanda Pereira da Costa2022-04-29T08:46:36Z2022-04-29T08:46:36Z2022-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.aquatox.2022.106104Aquatic Toxicology, v. 245.1879-15140166-445Xhttp://hdl.handle.net/11449/23161610.1016/j.aquatox.2022.1061042-s2.0-85124461420Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquatic Toxicologyinfo:eu-repo/semantics/openAccess2022-04-29T08:46:36Zoai:repositorio.unesp.br:11449/231616Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:55:05.557405Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Toxicological impact of SARS-CoV-2 on the health of the neotropical fish, Poecilia reticulata
title Toxicological impact of SARS-CoV-2 on the health of the neotropical fish, Poecilia reticulata
spellingShingle Toxicological impact of SARS-CoV-2 on the health of the neotropical fish, Poecilia reticulata
Malafaia, Guilherme
Behavior
COVID-19
Fish
REDOX unbalance
Viral particles
Water pollution
title_short Toxicological impact of SARS-CoV-2 on the health of the neotropical fish, Poecilia reticulata
title_full Toxicological impact of SARS-CoV-2 on the health of the neotropical fish, Poecilia reticulata
title_fullStr Toxicological impact of SARS-CoV-2 on the health of the neotropical fish, Poecilia reticulata
title_full_unstemmed Toxicological impact of SARS-CoV-2 on the health of the neotropical fish, Poecilia reticulata
title_sort Toxicological impact of SARS-CoV-2 on the health of the neotropical fish, Poecilia reticulata
author Malafaia, Guilherme
author_facet Malafaia, Guilherme
Ahmed, Mohamed Ahmed Ibrahim
Souza, Sindoval Silva de
Rezende, Fernanda Neves Estrela
Freitas, Ítalo Nascimento
da Luz, Thiarlen Marinho
da Silva, Abner Marcelino
Charlie-Silva, Ives
Braz, Helyson Lucas Bezerra
Jorge, Roberta Jeane Bezerra
Sanches, Paulo R.S. [UNESP]
Mendonça-Gomes, Juliana Moreira
Cilli, Eduardo M. [UNESP]
Araújo, Amanda Pereira da Costa
author_role author
author2 Ahmed, Mohamed Ahmed Ibrahim
Souza, Sindoval Silva de
Rezende, Fernanda Neves Estrela
Freitas, Ítalo Nascimento
da Luz, Thiarlen Marinho
da Silva, Abner Marcelino
Charlie-Silva, Ives
Braz, Helyson Lucas Bezerra
Jorge, Roberta Jeane Bezerra
Sanches, Paulo R.S. [UNESP]
Mendonça-Gomes, Juliana Moreira
Cilli, Eduardo M. [UNESP]
Araújo, Amanda Pereira da Costa
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Goiano Federal Institute
Universidade Federal de Goiás (UFG)
Universidade Federal de Uberlândia (UFU)
Assiut University
Universidade de São Paulo (USP)
Federal University of Ceará
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Malafaia, Guilherme
Ahmed, Mohamed Ahmed Ibrahim
Souza, Sindoval Silva de
Rezende, Fernanda Neves Estrela
Freitas, Ítalo Nascimento
da Luz, Thiarlen Marinho
da Silva, Abner Marcelino
Charlie-Silva, Ives
Braz, Helyson Lucas Bezerra
Jorge, Roberta Jeane Bezerra
Sanches, Paulo R.S. [UNESP]
Mendonça-Gomes, Juliana Moreira
Cilli, Eduardo M. [UNESP]
Araújo, Amanda Pereira da Costa
dc.subject.por.fl_str_mv Behavior
COVID-19
Fish
REDOX unbalance
Viral particles
Water pollution
topic Behavior
COVID-19
Fish
REDOX unbalance
Viral particles
Water pollution
description There have been significant impacts of the current COVID-19 pandemic on society including high health and economic costs. However, little is known about the potential ecological risks of this virus despite its presence in freshwater systems. In this study, we aimed to evaluate the exposure of Poecilia reticulata juveniles to two peptides derived from Spike protein of SARS-CoV-2, which was synthesized in the laboratory (named PSPD-2002 and PSPD-2003). For this, the animals were exposed for 35 days to the peptides at a concentration of 40 µg/L and different toxicity biomarkers were assessed. Our data indicated that the peptides were able to induce anxiety-like behavior in the open field test and increased acetylcholinesterase (AChE) activity. The biometric evaluation also revealed that the animals exposed to the peptides displayed alterations in the pattern of growth/development. Furthermore, the increased activity of superoxide dismutase (SOD) and catalase (CAT) enzymes were accompanied by increased levels of malondialdehyde (MDA), reactive oxygen species (ROS) and hydrogen peroxide (H2O2), which suggests a redox imbalance induced by SARS-CoV-2 spike protein peptides. Moreover, molecular docking analysis suggested a strong interaction of the peptides with the enzymes AChE, SOD and CAT, allowing us to infer that the observed effects are related to the direct action of the peptides on the functionality of these enzymes. Consequently, our study provided evidence that the presence of SARS-CoV-2 viral particles in the freshwater ecosystems offer a health risk to fish and other aquatic organisms.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:46:36Z
2022-04-29T08:46:36Z
2022-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.aquatox.2022.106104
Aquatic Toxicology, v. 245.
1879-1514
0166-445X
http://hdl.handle.net/11449/231616
10.1016/j.aquatox.2022.106104
2-s2.0-85124461420
url http://dx.doi.org/10.1016/j.aquatox.2022.106104
http://hdl.handle.net/11449/231616
identifier_str_mv Aquatic Toxicology, v. 245.
1879-1514
0166-445X
10.1016/j.aquatox.2022.106104
2-s2.0-85124461420
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Aquatic Toxicology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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