Síntese de ciclotrifosfato de cálcio e seu efeito contra a erosão inicial do esmalte: estudo in vitro

• Autor(a) principal: Jesus, Anna Julha Souza de [UNESP]
• Data de Publicação: 2024
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: https://hdl.handle.net/11449/256144
• topic: Esmalte dentário; Fosfatos; Erosão dentária; Tooth erosion

The objective of the present study was to synthesize and characterize cyclotriphosphate (TMP) with total replacement of sodium by calcium, and to verify its effect on the initial erosion of enamel. Cyclotriphosphate microcompounds with total replacement of sodium by calcium (CaTMP) were synthesized and morphologically characterized using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). The enamel blocks (n = 98) were randomly distributed according to the average surface hardness (SH) of the total blocks and the confidence interval between 7 groups of experimental dentifrices containing 1% of the compounds: 0% CaTMP and 100% NaTMP (NaTMP), 20% CaTMP and 80% NaTMP (20% CaTMP), 50% CaTMP and 50% NaTMP (50 % CaTMP), 80% CaTMP and 20% NaTMP (80% CaTMP), and 100% CaTMP and 0% NaTMP (CaTMP). A placebo dentifrice was used as a negative control and a 1100 ppm F dentifrice (NaF, Merck, CAS 7681-49-4, Germany) was used as a positive control. To analyze the protective effect, the sound enamel blocks were immersed in human saliva for 2 hours (enhanced), followed by a dentifrice solution with human saliva for 2 minutes. Therefore, 4 erosive challenges were performed (citric acid, 0.75%, pH 3.5, for 1 minute, under effective). The percentage of variation in surface hardness (%SH) was calculated after treatment and fatty acid challenges of 1, 2, 3 and 4 minutes. The variables were subjected to two-way repeated measures analysis of variance followed by the Student-Newman-Keuls test (p<0.05). Toothpaste containing Ca-TMP showed the greatest protective effect compared to other groups (p <0.001). The protective factor was 100% CaTMP > 80% CaTMP > 50% CaTMP > 20% CaTMP > 1100 ppm F > NaTMP > Placebo (p < 0.001). The higher the percentage of CaTMP in the solutions, the greater the adsorption of CaTMP. All groups produced precipitates with a thicker layer for the CaTMP groups. It is concluded that toothpaste solutions containing CaTMP have a superior effect on initial erosive lesions of the enamel.