Hepatitis C Virus NS2 Protein Inhibits DNA Damage Pathway by Sequestering p53 to the Cytoplasm
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0062581 http://hdl.handle.net/11449/75191 |
Resumo: | Chronic hepatitis C virus (HCV) infection is an important cause of morbidity and mortality globally, and often leads to end-stage liver disease. The DNA damage checkpoint pathway induces cell cycle arrest for repairing DNA in response to DNA damage. HCV infection has been involved in this pathway. In this study, we assess the effects of HCV NS2 on DNA damage checkpoint pathway. We have observed that HCV NS2 induces ataxia-telangiectasia mutated checkpoint pathway by inducing Chk2, however, fails to activate the subsequent downstream pathway. Further study suggested that p53 is retained in the cytoplasm of HCV NS2 expressing cells, and p21 expression is not enhanced. We further observed that HCV NS2 expressing cells induce cyclin E expression and promote cell growth. Together these results suggested that HCV NS2 inhibits DNA damage response by altering the localization of p53, and may play a role in the pathogenesis of HCV infection. © 2013 Bitter et al. |
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Hepatitis C Virus NS2 Protein Inhibits DNA Damage Pathway by Sequestering p53 to the Cytoplasmcheckpoint kinase 2cyclin Enonstructural protein 2protein p21protein p53cell growthcell proliferationcontrolled studycytoplasmDNA damageHepatitis C virusnonhumanprotein expressionprotein functionprotein localizationprotein transportChronic hepatitis C virus (HCV) infection is an important cause of morbidity and mortality globally, and often leads to end-stage liver disease. The DNA damage checkpoint pathway induces cell cycle arrest for repairing DNA in response to DNA damage. HCV infection has been involved in this pathway. In this study, we assess the effects of HCV NS2 on DNA damage checkpoint pathway. We have observed that HCV NS2 induces ataxia-telangiectasia mutated checkpoint pathway by inducing Chk2, however, fails to activate the subsequent downstream pathway. Further study suggested that p53 is retained in the cytoplasm of HCV NS2 expressing cells, and p21 expression is not enhanced. We further observed that HCV NS2 expressing cells induce cyclin E expression and promote cell growth. Together these results suggested that HCV NS2 inhibits DNA damage response by altering the localization of p53, and may play a role in the pathogenesis of HCV infection. © 2013 Bitter et al.Department of Pathology Saint Louis University, St. Louis, MODeaprtment of Biology UNESP - São Paulo State University, São José do Rio Preto, São PauloDeaprtment of Biology UNESP - São Paulo State University, São José do Rio Preto, São PauloSaint Louis UniversityUniversidade Estadual Paulista (Unesp)Bittar, Cintia [UNESP]Shrivastava, ShubhamBhanja Chowdhury, JoydipRahal, Paula [UNESP]Ray, Ratna B.2014-05-27T11:29:00Z2014-05-27T11:29:00Z2013-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0062581PLoS ONE, v. 8, n. 4, 2013.1932-6203http://hdl.handle.net/11449/7519110.1371/journal.pone.0062581WOS:0003190773000772-s2.0-848769644112-s2.0-84876964411.pdf79910823626712120000-0001-5693-6148Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2023-10-10T06:04:51Zoai:repositorio.unesp.br:11449/75191Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:31:09.283230Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hepatitis C Virus NS2 Protein Inhibits DNA Damage Pathway by Sequestering p53 to the Cytoplasm |
title |
Hepatitis C Virus NS2 Protein Inhibits DNA Damage Pathway by Sequestering p53 to the Cytoplasm |
spellingShingle |
Hepatitis C Virus NS2 Protein Inhibits DNA Damage Pathway by Sequestering p53 to the Cytoplasm Bittar, Cintia [UNESP] checkpoint kinase 2 cyclin E nonstructural protein 2 protein p21 protein p53 cell growth cell proliferation controlled study cytoplasm DNA damage Hepatitis C virus nonhuman protein expression protein function protein localization protein transport |
title_short |
Hepatitis C Virus NS2 Protein Inhibits DNA Damage Pathway by Sequestering p53 to the Cytoplasm |
title_full |
Hepatitis C Virus NS2 Protein Inhibits DNA Damage Pathway by Sequestering p53 to the Cytoplasm |
title_fullStr |
Hepatitis C Virus NS2 Protein Inhibits DNA Damage Pathway by Sequestering p53 to the Cytoplasm |
title_full_unstemmed |
Hepatitis C Virus NS2 Protein Inhibits DNA Damage Pathway by Sequestering p53 to the Cytoplasm |
title_sort |
Hepatitis C Virus NS2 Protein Inhibits DNA Damage Pathway by Sequestering p53 to the Cytoplasm |
author |
Bittar, Cintia [UNESP] |
author_facet |
Bittar, Cintia [UNESP] Shrivastava, Shubham Bhanja Chowdhury, Joydip Rahal, Paula [UNESP] Ray, Ratna B. |
author_role |
author |
author2 |
Shrivastava, Shubham Bhanja Chowdhury, Joydip Rahal, Paula [UNESP] Ray, Ratna B. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Saint Louis University Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bittar, Cintia [UNESP] Shrivastava, Shubham Bhanja Chowdhury, Joydip Rahal, Paula [UNESP] Ray, Ratna B. |
dc.subject.por.fl_str_mv |
checkpoint kinase 2 cyclin E nonstructural protein 2 protein p21 protein p53 cell growth cell proliferation controlled study cytoplasm DNA damage Hepatitis C virus nonhuman protein expression protein function protein localization protein transport |
topic |
checkpoint kinase 2 cyclin E nonstructural protein 2 protein p21 protein p53 cell growth cell proliferation controlled study cytoplasm DNA damage Hepatitis C virus nonhuman protein expression protein function protein localization protein transport |
description |
Chronic hepatitis C virus (HCV) infection is an important cause of morbidity and mortality globally, and often leads to end-stage liver disease. The DNA damage checkpoint pathway induces cell cycle arrest for repairing DNA in response to DNA damage. HCV infection has been involved in this pathway. In this study, we assess the effects of HCV NS2 on DNA damage checkpoint pathway. We have observed that HCV NS2 induces ataxia-telangiectasia mutated checkpoint pathway by inducing Chk2, however, fails to activate the subsequent downstream pathway. Further study suggested that p53 is retained in the cytoplasm of HCV NS2 expressing cells, and p21 expression is not enhanced. We further observed that HCV NS2 expressing cells induce cyclin E expression and promote cell growth. Together these results suggested that HCV NS2 inhibits DNA damage response by altering the localization of p53, and may play a role in the pathogenesis of HCV infection. © 2013 Bitter et al. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04-30 2014-05-27T11:29:00Z 2014-05-27T11:29:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0062581 PLoS ONE, v. 8, n. 4, 2013. 1932-6203 http://hdl.handle.net/11449/75191 10.1371/journal.pone.0062581 WOS:000319077300077 2-s2.0-84876964411 2-s2.0-84876964411.pdf 7991082362671212 0000-0001-5693-6148 |
url |
http://dx.doi.org/10.1371/journal.pone.0062581 http://hdl.handle.net/11449/75191 |
identifier_str_mv |
PLoS ONE, v. 8, n. 4, 2013. 1932-6203 10.1371/journal.pone.0062581 WOS:000319077300077 2-s2.0-84876964411 2-s2.0-84876964411.pdf 7991082362671212 0000-0001-5693-6148 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLOS ONE 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128372792885248 |