Congenital toxoplasmosis in chronically infected and subsequently challenged ewes
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0165124 http://hdl.handle.net/11449/173699 |
Resumo: | This experiment studied congenital transmission in sheep experimentally infected with oocysts of Toxoplasma gondii and reinfected at one of three stages of pregnancy. Twenty ewes were experimentally infected with T. gondii strain ME49 (day 0). After the T. gondii infection became chronic (IFAT≤512), the ewes were allocated with rams for coverage. After the diagnosis of pregnancy, these ewes were allocated into four experimental groups (n = 5): I-reinfected with T. gondii on the 40th day of gestation (DG); II-reinfected on DG 80; III-reinfected on DG 120; and IV-saline solution on DG 120 (not reinfected). Five ewes (IFAT<64) were kept as negative controls (uninfected, group V), therefore in groups I-III were infected prior to pregnancy and re-infected during pregnancy, group IV was only infected prior to pregnancy, and group V was not infected. Parasitism by T. gondii was investigated (histopathology, immunohistochemistry, mouse bioassay and PCR) in mothers and lambs tissue. All ewes produced lambs serologically positive for T. gondii. The results of the mouse bioassay, immunohistochemistry and PCR assays revealed the presence of T. gondii in all 20 sheep and their lambs. The congenital transmission of T. gondii was associated with fetal loss and abnormalities in persistently infected sheep and in ewes infected and subsequently reinfected by this protozoan. Therefore, congenital T. gondii infection was common when ewes were chronically infected prior to pregnancy, with or without reinfection during at various stages of gestation. |
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Congenital toxoplasmosis in chronically infected and subsequently challenged ewesThis experiment studied congenital transmission in sheep experimentally infected with oocysts of Toxoplasma gondii and reinfected at one of three stages of pregnancy. Twenty ewes were experimentally infected with T. gondii strain ME49 (day 0). After the T. gondii infection became chronic (IFAT≤512), the ewes were allocated with rams for coverage. After the diagnosis of pregnancy, these ewes were allocated into four experimental groups (n = 5): I-reinfected with T. gondii on the 40th day of gestation (DG); II-reinfected on DG 80; III-reinfected on DG 120; and IV-saline solution on DG 120 (not reinfected). Five ewes (IFAT<64) were kept as negative controls (uninfected, group V), therefore in groups I-III were infected prior to pregnancy and re-infected during pregnancy, group IV was only infected prior to pregnancy, and group V was not infected. Parasitism by T. gondii was investigated (histopathology, immunohistochemistry, mouse bioassay and PCR) in mothers and lambs tissue. All ewes produced lambs serologically positive for T. gondii. The results of the mouse bioassay, immunohistochemistry and PCR assays revealed the presence of T. gondii in all 20 sheep and their lambs. The congenital transmission of T. gondii was associated with fetal loss and abnormalities in persistently infected sheep and in ewes infected and subsequently reinfected by this protozoan. Therefore, congenital T. gondii infection was common when ewes were chronically infected prior to pregnancy, with or without reinfection during at various stages of gestation.Department of Veterinary Medicine Rondônia Federal University (UNIR) Rolim de Moura, Av. Norte-Sul, 7300Department of Animal Pathology CPPAR FCAV UNESP - Jaboticabal, Access road, Prof. Paul Donato Castellane s/nDepartment of Preventive Veterinary Medicine UELDepartment of Clinic Surgery and Animal Reproduction FMVA UNESPDepartment of Production and Animal Health FMVA UNESPDepartment of Animal Pathology CPPAR FCAV UNESP - Jaboticabal, Access road, Prof. Paul Donato Castellane s/nDepartment of Clinic Surgery and Animal Reproduction FMVA UNESPDepartment of Production and Animal Health FMVA UNESPRolim de MouraUniversidade Estadual Paulista (Unesp)UELDos Santos, Thaís RabeloFaria, Gabriela Da Silva Magalhães [UNESP]Guerreiro, Bruna Martins [UNESP]Dal Pietro, Nathalia Helena Pereira Da Silva [UNESP]Lopes, Welber Daniel Zanetti [UNESP]Da Silva, Helenara Machado [UNESP]Garcia, João LuisLuvizotto, Maria Cecília Rui [UNESP]Bresciani, Katia Denise Saraiva [UNESP]Da Costa, Alvimar José [UNESP]2018-12-11T17:07:21Z2018-12-11T17:07:21Z2016-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0165124PLoS ONE, v. 11, n. 10, 2016.1932-6203http://hdl.handle.net/11449/17369910.1371/journal.pone.01651242-s2.0-849927092312-s2.0-84992709231.pdf59505943668296474584674909952477Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONE1,164info:eu-repo/semantics/openAccess2024-09-04T18:03:58Zoai:repositorio.unesp.br:11449/173699Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:03:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Congenital toxoplasmosis in chronically infected and subsequently challenged ewes |
title |
Congenital toxoplasmosis in chronically infected and subsequently challenged ewes |
spellingShingle |
Congenital toxoplasmosis in chronically infected and subsequently challenged ewes Dos Santos, Thaís Rabelo |
title_short |
Congenital toxoplasmosis in chronically infected and subsequently challenged ewes |
title_full |
Congenital toxoplasmosis in chronically infected and subsequently challenged ewes |
title_fullStr |
Congenital toxoplasmosis in chronically infected and subsequently challenged ewes |
title_full_unstemmed |
Congenital toxoplasmosis in chronically infected and subsequently challenged ewes |
title_sort |
Congenital toxoplasmosis in chronically infected and subsequently challenged ewes |
author |
Dos Santos, Thaís Rabelo |
author_facet |
Dos Santos, Thaís Rabelo Faria, Gabriela Da Silva Magalhães [UNESP] Guerreiro, Bruna Martins [UNESP] Dal Pietro, Nathalia Helena Pereira Da Silva [UNESP] Lopes, Welber Daniel Zanetti [UNESP] Da Silva, Helenara Machado [UNESP] Garcia, João Luis Luvizotto, Maria Cecília Rui [UNESP] Bresciani, Katia Denise Saraiva [UNESP] Da Costa, Alvimar José [UNESP] |
author_role |
author |
author2 |
Faria, Gabriela Da Silva Magalhães [UNESP] Guerreiro, Bruna Martins [UNESP] Dal Pietro, Nathalia Helena Pereira Da Silva [UNESP] Lopes, Welber Daniel Zanetti [UNESP] Da Silva, Helenara Machado [UNESP] Garcia, João Luis Luvizotto, Maria Cecília Rui [UNESP] Bresciani, Katia Denise Saraiva [UNESP] Da Costa, Alvimar José [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Rolim de Moura Universidade Estadual Paulista (Unesp) UEL |
dc.contributor.author.fl_str_mv |
Dos Santos, Thaís Rabelo Faria, Gabriela Da Silva Magalhães [UNESP] Guerreiro, Bruna Martins [UNESP] Dal Pietro, Nathalia Helena Pereira Da Silva [UNESP] Lopes, Welber Daniel Zanetti [UNESP] Da Silva, Helenara Machado [UNESP] Garcia, João Luis Luvizotto, Maria Cecília Rui [UNESP] Bresciani, Katia Denise Saraiva [UNESP] Da Costa, Alvimar José [UNESP] |
description |
This experiment studied congenital transmission in sheep experimentally infected with oocysts of Toxoplasma gondii and reinfected at one of three stages of pregnancy. Twenty ewes were experimentally infected with T. gondii strain ME49 (day 0). After the T. gondii infection became chronic (IFAT≤512), the ewes were allocated with rams for coverage. After the diagnosis of pregnancy, these ewes were allocated into four experimental groups (n = 5): I-reinfected with T. gondii on the 40th day of gestation (DG); II-reinfected on DG 80; III-reinfected on DG 120; and IV-saline solution on DG 120 (not reinfected). Five ewes (IFAT<64) were kept as negative controls (uninfected, group V), therefore in groups I-III were infected prior to pregnancy and re-infected during pregnancy, group IV was only infected prior to pregnancy, and group V was not infected. Parasitism by T. gondii was investigated (histopathology, immunohistochemistry, mouse bioassay and PCR) in mothers and lambs tissue. All ewes produced lambs serologically positive for T. gondii. The results of the mouse bioassay, immunohistochemistry and PCR assays revealed the presence of T. gondii in all 20 sheep and their lambs. The congenital transmission of T. gondii was associated with fetal loss and abnormalities in persistently infected sheep and in ewes infected and subsequently reinfected by this protozoan. Therefore, congenital T. gondii infection was common when ewes were chronically infected prior to pregnancy, with or without reinfection during at various stages of gestation. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-01 2018-12-11T17:07:21Z 2018-12-11T17:07:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0165124 PLoS ONE, v. 11, n. 10, 2016. 1932-6203 http://hdl.handle.net/11449/173699 10.1371/journal.pone.0165124 2-s2.0-84992709231 2-s2.0-84992709231.pdf 5950594366829647 4584674909952477 |
url |
http://dx.doi.org/10.1371/journal.pone.0165124 http://hdl.handle.net/11449/173699 |
identifier_str_mv |
PLoS ONE, v. 11, n. 10, 2016. 1932-6203 10.1371/journal.pone.0165124 2-s2.0-84992709231 2-s2.0-84992709231.pdf 5950594366829647 4584674909952477 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021396255866880 |