Congenital toxoplasmosis in chronically infected and subsequently challenged ewes

Detalhes bibliográficos
Autor(a) principal: Dos Santos, Thaís Rabelo
Data de Publicação: 2016
Outros Autores: Faria, Gabriela Da Silva Magalhães [UNESP], Guerreiro, Bruna Martins [UNESP], Dal Pietro, Nathalia Helena Pereira Da Silva [UNESP], Lopes, Welber Daniel Zanetti [UNESP], Da Silva, Helenara Machado [UNESP], Garcia, João Luis, Luvizotto, Maria Cecília Rui [UNESP], Bresciani, Katia Denise Saraiva [UNESP], Da Costa, Alvimar José [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0165124
http://hdl.handle.net/11449/173699
Resumo: This experiment studied congenital transmission in sheep experimentally infected with oocysts of Toxoplasma gondii and reinfected at one of three stages of pregnancy. Twenty ewes were experimentally infected with T. gondii strain ME49 (day 0). After the T. gondii infection became chronic (IFAT≤512), the ewes were allocated with rams for coverage. After the diagnosis of pregnancy, these ewes were allocated into four experimental groups (n = 5): I-reinfected with T. gondii on the 40th day of gestation (DG); II-reinfected on DG 80; III-reinfected on DG 120; and IV-saline solution on DG 120 (not reinfected). Five ewes (IFAT<64) were kept as negative controls (uninfected, group V), therefore in groups I-III were infected prior to pregnancy and re-infected during pregnancy, group IV was only infected prior to pregnancy, and group V was not infected. Parasitism by T. gondii was investigated (histopathology, immunohistochemistry, mouse bioassay and PCR) in mothers and lambs tissue. All ewes produced lambs serologically positive for T. gondii. The results of the mouse bioassay, immunohistochemistry and PCR assays revealed the presence of T. gondii in all 20 sheep and their lambs. The congenital transmission of T. gondii was associated with fetal loss and abnormalities in persistently infected sheep and in ewes infected and subsequently reinfected by this protozoan. Therefore, congenital T. gondii infection was common when ewes were chronically infected prior to pregnancy, with or without reinfection during at various stages of gestation.
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spelling Congenital toxoplasmosis in chronically infected and subsequently challenged ewesThis experiment studied congenital transmission in sheep experimentally infected with oocysts of Toxoplasma gondii and reinfected at one of three stages of pregnancy. Twenty ewes were experimentally infected with T. gondii strain ME49 (day 0). After the T. gondii infection became chronic (IFAT≤512), the ewes were allocated with rams for coverage. After the diagnosis of pregnancy, these ewes were allocated into four experimental groups (n = 5): I-reinfected with T. gondii on the 40th day of gestation (DG); II-reinfected on DG 80; III-reinfected on DG 120; and IV-saline solution on DG 120 (not reinfected). Five ewes (IFAT<64) were kept as negative controls (uninfected, group V), therefore in groups I-III were infected prior to pregnancy and re-infected during pregnancy, group IV was only infected prior to pregnancy, and group V was not infected. Parasitism by T. gondii was investigated (histopathology, immunohistochemistry, mouse bioassay and PCR) in mothers and lambs tissue. All ewes produced lambs serologically positive for T. gondii. The results of the mouse bioassay, immunohistochemistry and PCR assays revealed the presence of T. gondii in all 20 sheep and their lambs. The congenital transmission of T. gondii was associated with fetal loss and abnormalities in persistently infected sheep and in ewes infected and subsequently reinfected by this protozoan. Therefore, congenital T. gondii infection was common when ewes were chronically infected prior to pregnancy, with or without reinfection during at various stages of gestation.Department of Veterinary Medicine Rondônia Federal University (UNIR) Rolim de Moura, Av. Norte-Sul, 7300Department of Animal Pathology CPPAR FCAV UNESP - Jaboticabal, Access road, Prof. Paul Donato Castellane s/nDepartment of Preventive Veterinary Medicine UELDepartment of Clinic Surgery and Animal Reproduction FMVA UNESPDepartment of Production and Animal Health FMVA UNESPDepartment of Animal Pathology CPPAR FCAV UNESP - Jaboticabal, Access road, Prof. Paul Donato Castellane s/nDepartment of Clinic Surgery and Animal Reproduction FMVA UNESPDepartment of Production and Animal Health FMVA UNESPRolim de MouraUniversidade Estadual Paulista (Unesp)UELDos Santos, Thaís RabeloFaria, Gabriela Da Silva Magalhães [UNESP]Guerreiro, Bruna Martins [UNESP]Dal Pietro, Nathalia Helena Pereira Da Silva [UNESP]Lopes, Welber Daniel Zanetti [UNESP]Da Silva, Helenara Machado [UNESP]Garcia, João LuisLuvizotto, Maria Cecília Rui [UNESP]Bresciani, Katia Denise Saraiva [UNESP]Da Costa, Alvimar José [UNESP]2018-12-11T17:07:21Z2018-12-11T17:07:21Z2016-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0165124PLoS ONE, v. 11, n. 10, 2016.1932-6203http://hdl.handle.net/11449/17369910.1371/journal.pone.01651242-s2.0-849927092312-s2.0-84992709231.pdf59505943668296474584674909952477Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONE1,164info:eu-repo/semantics/openAccess2024-09-04T18:03:58Zoai:repositorio.unesp.br:11449/173699Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:03:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Congenital toxoplasmosis in chronically infected and subsequently challenged ewes
title Congenital toxoplasmosis in chronically infected and subsequently challenged ewes
spellingShingle Congenital toxoplasmosis in chronically infected and subsequently challenged ewes
Dos Santos, Thaís Rabelo
title_short Congenital toxoplasmosis in chronically infected and subsequently challenged ewes
title_full Congenital toxoplasmosis in chronically infected and subsequently challenged ewes
title_fullStr Congenital toxoplasmosis in chronically infected and subsequently challenged ewes
title_full_unstemmed Congenital toxoplasmosis in chronically infected and subsequently challenged ewes
title_sort Congenital toxoplasmosis in chronically infected and subsequently challenged ewes
author Dos Santos, Thaís Rabelo
author_facet Dos Santos, Thaís Rabelo
Faria, Gabriela Da Silva Magalhães [UNESP]
Guerreiro, Bruna Martins [UNESP]
Dal Pietro, Nathalia Helena Pereira Da Silva [UNESP]
Lopes, Welber Daniel Zanetti [UNESP]
Da Silva, Helenara Machado [UNESP]
Garcia, João Luis
Luvizotto, Maria Cecília Rui [UNESP]
Bresciani, Katia Denise Saraiva [UNESP]
Da Costa, Alvimar José [UNESP]
author_role author
author2 Faria, Gabriela Da Silva Magalhães [UNESP]
Guerreiro, Bruna Martins [UNESP]
Dal Pietro, Nathalia Helena Pereira Da Silva [UNESP]
Lopes, Welber Daniel Zanetti [UNESP]
Da Silva, Helenara Machado [UNESP]
Garcia, João Luis
Luvizotto, Maria Cecília Rui [UNESP]
Bresciani, Katia Denise Saraiva [UNESP]
Da Costa, Alvimar José [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Rolim de Moura
Universidade Estadual Paulista (Unesp)
UEL
dc.contributor.author.fl_str_mv Dos Santos, Thaís Rabelo
Faria, Gabriela Da Silva Magalhães [UNESP]
Guerreiro, Bruna Martins [UNESP]
Dal Pietro, Nathalia Helena Pereira Da Silva [UNESP]
Lopes, Welber Daniel Zanetti [UNESP]
Da Silva, Helenara Machado [UNESP]
Garcia, João Luis
Luvizotto, Maria Cecília Rui [UNESP]
Bresciani, Katia Denise Saraiva [UNESP]
Da Costa, Alvimar José [UNESP]
description This experiment studied congenital transmission in sheep experimentally infected with oocysts of Toxoplasma gondii and reinfected at one of three stages of pregnancy. Twenty ewes were experimentally infected with T. gondii strain ME49 (day 0). After the T. gondii infection became chronic (IFAT≤512), the ewes were allocated with rams for coverage. After the diagnosis of pregnancy, these ewes were allocated into four experimental groups (n = 5): I-reinfected with T. gondii on the 40th day of gestation (DG); II-reinfected on DG 80; III-reinfected on DG 120; and IV-saline solution on DG 120 (not reinfected). Five ewes (IFAT<64) were kept as negative controls (uninfected, group V), therefore in groups I-III were infected prior to pregnancy and re-infected during pregnancy, group IV was only infected prior to pregnancy, and group V was not infected. Parasitism by T. gondii was investigated (histopathology, immunohistochemistry, mouse bioassay and PCR) in mothers and lambs tissue. All ewes produced lambs serologically positive for T. gondii. The results of the mouse bioassay, immunohistochemistry and PCR assays revealed the presence of T. gondii in all 20 sheep and their lambs. The congenital transmission of T. gondii was associated with fetal loss and abnormalities in persistently infected sheep and in ewes infected and subsequently reinfected by this protozoan. Therefore, congenital T. gondii infection was common when ewes were chronically infected prior to pregnancy, with or without reinfection during at various stages of gestation.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-01
2018-12-11T17:07:21Z
2018-12-11T17:07:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0165124
PLoS ONE, v. 11, n. 10, 2016.
1932-6203
http://hdl.handle.net/11449/173699
10.1371/journal.pone.0165124
2-s2.0-84992709231
2-s2.0-84992709231.pdf
5950594366829647
4584674909952477
url http://dx.doi.org/10.1371/journal.pone.0165124
http://hdl.handle.net/11449/173699
identifier_str_mv PLoS ONE, v. 11, n. 10, 2016.
1932-6203
10.1371/journal.pone.0165124
2-s2.0-84992709231
2-s2.0-84992709231.pdf
5950594366829647
4584674909952477
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS ONE
1,164
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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